MAR 27, 2018 9:00 AM PDT

How to use CRISPR-Cas9 for knockout, knock-in and gene activation

Sponsored by: Horizon Discovery
Speaker
  • Product Manager, Horizon Discovery
    Biography
      Amanda Haas is a Product Manager at Horizon Discovery. Amanda joined Dharmacon (now a Horizon Discovery group company) in 2005 and has worked in multiple departments including Manufacturing, Chemistry R&D, and Biology R&D. She is responsible for custom synthetic manufacturing and Cas9 nuclease products, she has contributed to research projects and new product development in multiple areas across RNA interference and CRISPR-Cas9 genome engineering. She received her degree in Chemistry and Religion at The University of Miami in 2003.

    Abstract

    DATE: March 27, 2018
    TIME: 09:00am PDT, 12:00pm EDT

    The rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, and transcriptional regulation. This allows researchers to more quickly define the role of their gene in various biological contexts to progress the understanding of human disease. The wide availability of genome engineering tools makes these applications highly accessible, but still requires careful consideration for reagent selection, design, and experimental optimization. In this webinar, we will demonstrate applications of the CRISPR-Cas9 system for creation of a knockout cell line, precise knock-in, and activation of gene transcription.

    Learning Objectives:

    • Learn how to successfully generate and characterize knockout cell lines
    • Recognize the key factors for successful fluorescent knock-in, as demonstrated in K562 and iPS cell lines
    • Understand how to use adapted CRISPR-Cas9 reagents for robust activation of one or more genes for gain-of-function studies
    • Discover how our design tools, reagents, and services can help with your knockout, knock-in, or gene activation experiments

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