SEP 30, 2020 12:00 PM PDT

Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA

Presented at: CRISPR 2020
Sponsored by: MilliporeSigma
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Head of Business for EMEA, Merck KGaA
    Biography

      Dr. Gurpreet Balrey is the Head of Business for Europe, Middle East and Africa for the Genome Engineering and Modulation franchise at Merck. He attended the University of Nottingham for a Biotechnology degree followed by a PhD in Molecular Biology and Plant Genetic Engineering at the same University. Gurpreet has held various positions within the technical sales team during his 11 years with the company. He has particularly been involved in working with the Welcome Trust Sanger Institute to bring the arrayed whole genome libraries to market and more currently on our SygRNA® synthetic two part and single guide CRISPR RNA offering.

    • Managing Director of the Genome Editing Core Facility; Rutgers Cancer Institute of New Jersey
      Biography

        Dr. Peter Romanienko obtained his PhD training from Cornell Graduate School of Medical Sciences/Sloan Kettering Institute working on DNA repair in Dr. Maria Jasin's laboratory and then continued working on mechanisms of DNA damage response in vivo in Dan Camerini-Otero's laboratory at NIH. After the NIH, he joined Memorial Sloan Kettering Cancer Center (MSKCC) and instituted a comprehensive mouse colony management service and supported the generation of new GEMMs. While at MSKCC, he introduced the use of gene editing to make mouse models, first with TALENS and then CRISPR-Cas9. After gaining extensive experience applying these novel methods that have revolutionized modern research, he moved to Rutgers Cancer Institute to setup and run the Genome Editing Shared Resource (GESR). While at Rutgers, the GESR has generated well over 120 novel mouse lines using CRISPR-Cas9 and also works to develop and introduce new methodologies.


      Abstract

      The CRISPR/Cas genome editing system has revolutionized nearly every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA, or lentivirus. Synthetic single modified guide RNA (sgRNA) delivered pre-complexed with Cas nucleases reduces the limiting factors of the above formats such as off-target effects, low efficiency, and undesired cellular responses to foreign DNA.  Discover how the Genome Editing Shared Resource at Rutgers Cancer Institute vetted and validated the transition from in vitro transcribed CRISPR sgRNA/Cas9 mRNA to synthetic two-part CRISPR RNA:trans-activating CRISPR RNA (crRNA:tracrRNA) and Cas9 protein and then to full-length synthetic sgRNA ribonucleoprotein (RNP) complexes. Applications of sgRNA in animal model generation and cell line applications will be discussed.

      Learning Objectives:

      1. Best practices for using synthetic sgRNA for mouse model generation

      2. How to use sgRNA RNPs for a variety of cell-based applications


      Show Resources
      You May Also Like
      MAY 11, 2021 10:00 AM PDT
      C.E. CREDITS
      MAY 11, 2021 10:00 AM PDT
      Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
      NOV 16, 2020 8:00 AM PST
      C.E. CREDITS
      NOV 16, 2020 8:00 AM PST
      Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
      JUN 09, 2021 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      JUN 09, 2021 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
      NOV 18, 2020 8:00 AM PST
      C.E. CREDITS
      NOV 18, 2020 8:00 AM PST
      DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
      MAR 16, 2021 10:00 AM PDT
      C.E. CREDITS
      MAR 16, 2021 10:00 AM PDT
      Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
      DEC 02, 2020 8:00 AM PST
      C.E. CREDITS
      DEC 02, 2020 8:00 AM PST
      DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
      Loading Comments...
      Show Resources
      Attendees
      • See more