MAY 17, 2018 09:00 AM PDT

Efficient purification of recombinant Strep-tag® fusion proteins from Expi supernatants using Strep-Tactin®XT

Speakers
  • Scientist, IBA Life Sciences
    Biography
      Dennis Niermeier received his master's degree in biotechnology from the University of Applied Sciences in Bremerhaven. During his master's study at the department of Pharmaceutical Biotechnology at the Fraunhofer Institute for Toxicology and Experimental Medicine, he worked on the development of a protein purification platform and in cell line development. In 2012, Dennis Niermeier joined IBA GmbH. He is responsible for the mammalian custom protein expression & purification service and the product development in this field.

    Abstract:

    Mammalian transient expression has become an effective method to provide recombinant proteins in a short time. Therefore, mammalian transient expression systems are applied to a great extend in R&D departments of pharmaceutical companies and academia to screen drug candidates and proteins. The Expi Expression System enables high transient protein titers and reduces the expression scale as well as the expression time. Affinity tags simplify protein purification and support the high throughput process by using the same purification strategy for many different proteins. However, many affinity tags have remarkable drawbacks like inconsistent purity, low binding capacity or large sizes. IBA´s Strep-tag® purification system (Strep-Tactin®XT:Twin-Strep-tag®) enables protein purification at high yields and purity under physiological conditions from several hosts following a simple one-step purification protocol. Providing the highest binding affinity among all affinity tag systems on the market, it is also well suited for many downstream applications like e.g. SPR. Due to its performance it complies well with the demands of a high throughput process.

    The webinar introduces the Strep-Tactin®XT technology and demonstrates the efficient and easy purification of Strep-tag® proteins from Expi supernatants. Additionally, it delivers an insight into the use of Strep-Tactin®XT for SPR analysis.


    Show Resources
    You May Also Like
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    SEP 05, 2019 04:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 04:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    JUN 19, 2019 10:00 AM PDT
    JUN 19, 2019 10:00 AM PDT
    DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    NOV 18, 2019 07:00 AM PST
    C.E. CREDITS
    NOV 18, 2019 07:00 AM PST
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    AUG 13, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 13, 2019 09:00 AM PDT
    DATE: August 13, 2019TIME: 9:00am PT, 12:00pm ET, 5:00pm BST Molecular complexes are major constituents of cells, hence unraveling their mechanisms is key to fuller comprehension of c...
    Loading Comments...
    Show Resources