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MAY 17, 2018 9:00 AM PDT

Efficient purification of recombinant Strep-tag® fusion proteins from Expi supernatants using Strep-Tactin®XT

Speaker
  • Scientist, IBA Life Sciences
    Biography
      Dennis Niermeier received his master's degree in biotechnology from the University of Applied Sciences in Bremerhaven. During his master's study at the department of Pharmaceutical Biotechnology at the Fraunhofer Institute for Toxicology and Experimental Medicine, he worked on the development of a protein purification platform and in cell line development. In 2012, Dennis Niermeier joined IBA GmbH. He is responsible for the mammalian custom protein expression & purification service and the product development in this field.

    Abstract

    Mammalian transient expression has become an effective method to provide recombinant proteins in a short time. Therefore, mammalian transient expression systems are applied to a great extend in R&D departments of pharmaceutical companies and academia to screen drug candidates and proteins. The Expi Expression System enables high transient protein titers and reduces the expression scale as well as the expression time. Affinity tags simplify protein purification and support the high throughput process by using the same purification strategy for many different proteins. However, many affinity tags have remarkable drawbacks like inconsistent purity, low binding capacity or large sizes. IBA´s Strep-tag® purification system (Strep-Tactin®XT:Twin-Strep-tag®) enables protein purification at high yields and purity under physiological conditions from several hosts following a simple one-step purification protocol. Providing the highest binding affinity among all affinity tag systems on the market, it is also well suited for many downstream applications like e.g. SPR. Due to its performance it complies well with the demands of a high throughput process.

    The webinar introduces the Strep-Tactin®XT technology and demonstrates the efficient and easy purification of Strep-tag® proteins from Expi supernatants. Additionally, it delivers an insight into the use of Strep-Tactin®XT for SPR analysis.


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