Expression, Purification and Characterization of Difficult to Express Membrane Proteins in the Expi293 and ExpiCHO Expression Systems: Case study of Potassium chloride co-transporter KCC2

Sponsored by: Thermo Fisher Scientific/Gibco

Speaker

Event Date & Time

DATE: September 25, 2019

TIME: 8:00am PDT, 11:00am EDT

Abstract

This talk will focus on strategies for optimizing expression levels of membrane proteins using the Gibco™ Expi293™ and ExpiCHO™ Expression Systems. Additionally, there will be an introduction to newly developed Expi293 GNTI- and inducible cell lines, which can be combined with CALIXAR's patented technology, to improve quality and quantity of challenging membrane proteins. A case study of potassium chloride co-transporter (KCC2) production and characterization will be further described

Learning Objectives:

Understand techniques to optimize expression levels of membrane proteins
Understand how to improve quality and quantity of challenging membrane proteins using Expi293 GNTI- and inducible cell lines

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