SEP 27, 2018 10:30 AM PDT

Holotomography Techniques for 3D Label-Free Imaging of Live Cells and Tissues

Presented at: Cell Biology 2018
Speaker
  • Associate Professor, Department of Physics, KAIST, Director, Time-Reversal Mirror Creative Research Center, Co-Founder and CTO, Tomocube, Inc.
    Biography
      Paul is Associate Professor of Physics at KAIST. He earned a Ph.D. from Harvard-MIT Health Science and Technology. Dr. Park's area of research is digital holography and its applications for biology and medicine. He has published +120 peer-reviewed papers including 3 Nat Photon, 2 Nat Comm, 4 PRL, 4 PNAS papers. Also, he is a fellow in OSA, and an editor of Optics Express, Scientific Reports, Experimental Biology and Medicine, and Journal of Current Optics and Photonics. Two start-up companies with +40 employees have been created from his research (Tomocube, The.Wave.Talk)

    Abstract

    Holotomography (HT) uses laser interferometry to measure 3-D refractive index (RI) distribution. HT serves as a powerful tool for imaging small transparent objects, such as biological cells and tissues. HT is an optical analogous to X-ray computed tomography (CT); HT measured multiple 2-D holograms of a sample with various illumination angles, from which a 3-D RI distribution of the sample is reconstructed by inversely solving the wave equation. Unlike conventional fluorescence-based imaging techniques, HT provides label-free 3-D imaging capability. Without any fixation or labeling, 3-D images of live cells can be obtained with high spatial resolution (down to 110 nm). Furthermore, HT provides quantitative imaging capability: RI maps of a cell are precisely and quantitative measured, from which various cellular analysis can be followed. 

    We present the principle and applications of HT in various fields, including cell biology, microbiology, and hematology. Also, we also present the recently developed multimodal system which simultaneously measures the 3-D RI tomogram and 3-D fluorescence image of a sample, and enables unprecedented correlative and quantitative bioimaging capability. 


    Learning Objectives: 

    1. To understand the working principle of holotomography 
    2. To learn direct applications of holotomography in the field of biology and medicine

     

     


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