MAY 16, 2019 12:00 PM PDT

Isolation and expansion of mesenchymal stromal or stem cells (MSCs) for translational and clinical research

SPONSORED BY: Miltenyi Biotec
C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Project Manager R&D, Miltenyi Biotec
    Biography
      Kathrin Godthardt joined Miltenyi Biotec's R&D department in 2001. She received her Bachelor's Degree from University of Mainz and finished her thesis on characterization of endothelia progenitor cells at Miltenyi Biotec. She has a long-term experience on separation technologies, development of cell culture media and adaptation of separation and cultivation processes into clinical-scale closed systems for mesenchymal stem cells. Currently she is a Team Coordinator and Project Manager of the R&D Stem cell and Regenerative Medicine Group.

    Abstract:
    DATE:  May 16, 2019
    TIME:   12:00 PM PDT
    Human mesenchymal stem cells (MSCs) hold great promise for clinical use and cell therapy applications. To ensure highest quality and safety of the resulting cellular products, MSCs have to be maintained using standardized cultivation conditions and procedures. To this end, we have developed our xeno-free MSC-Brew GMP Medium following the recommendations of USP <1043> on ancillary materials, thus enabling isolation and expansion of MSCs from various tissue sources (e.g. human bone marrow, adipose tissue, and umbilical cord). To increase the level of process standardization and product safety we developed a procedure using the integrated cell processing platform CliniMACS Prodigy® for i) density gradient centrifugation (DGC) of human bone marrow, ii) cultivation of MSCs starting from bone marrow mononuclear cells (BM-MNCs), and iii) passaging of MSCs. MSCs from human bone marrow were isolated by plastic adherence using MSC-Brew GMP Medium and automated feeding and harvesting procedures in a closed, single-use tubing set. Subsequently, these cells could be replated and expanded within the closed system, illustrating the feasibility of an automated cell production.
     
    Learning objectives:
    • Efficient MSCs expansion under serum-free and xeno-free conditions
    • Review Adherent Cell Culture (ACC) System using CliniMACS Prodigy® platform to acquire GMP-compliant and clinical scale MSCs
    • Fast and easy standardized characterization of MSCs via flow cytometry 
     
     
     
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