SEP 24, 2019 8:00 AM PDT

Interrogating 3D spheroid versus 2D monolayer cell models in drug discovery using cell health microplate assays

Sponsored by: Thermo Fisher Scientific
Speakers

Abstract

DATE: September 24, 2019
TIME: 8:00am PDT

High throughput screening (HTS) is an effective method for identifying putative active compounds for therapeutics. Assays that evaluate changes in cellular functions are essential for characterizing test compounds and their potential role as pharmaceuticals. In most cases, screening is facilitated by the readout of optical signals using microplate readers. Researchers in drug discovery and cancer research rely on reproducible biological assays to guide medicinal chemistry programs and lead optimization efforts. A current goal in cancer research is to expand the arsenal of quantitative assays that can be used to characterize and interrogate cellular functions in 3D models, which is currently very limited.

In this webinar, we will demonstrate the suitability of four different cell health assays (targeting 3 different cellular functions) to explore the cytotoxic effects of gambogic acid, used here as model compound given its known pleiotropic effects. We will demonstrate the suitability of these cell health microplate assays to examine differential responses of investigational compounds in 2D (monolayers) versus 3D (spheroids) models. These quantitative measurements are fast and simple to perform with the aid of a multimode microplate reader, such as the Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader. We will discuss in the webinar the principles of top versus bottom fluorescence measurements and their applicability in 3D versus 2D cellular models.

In summary, we will demonstrate that using existing microplate tools (that have been established for 2D cell models) in 3D cell models provides a time and cost efficient basis for understanding therapeutic effects on 3D cellular models. This allows for rapid therapeutic characterization in drug discovery with multimode microplate readers (such as Varioskan LUX multimode microplate reader) and permits side-by-side comparisons of 2D versus 3D cellular models without a need for additional assay optimization.

 

Learning Objectives:

  • Learn basic concepts related to optical detection of cellular functions using microplate readers
  • Become familiar with four different cell health assays and their benefits for performing screening as well as functional studies
  • Understand the applicability of cell health assays for the interrogation of cellular functions in 3D versus 2D cellular models

 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
JUL 15, 2021 8:00 AM PDT
C.E. CREDITS
JUL 15, 2021 8:00 AM PDT
Date: July 15, 2021 Time: 8:00am (PDT), 11:00am (EDT) High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Flu...
NOV 17, 2021 8:00 AM PST
C.E. CREDITS
NOV 17, 2021 8:00 AM PST
Date: November 17, 2021 Time: 8:00am (PDT), 11:00am (EDT) From waste disposal to promising biomarkers and therapeutic agents, exosomes and other extracellular vesicles are valuable in resear...
SEP 24, 2019 8:00 AM PDT

Interrogating 3D spheroid versus 2D monolayer cell models in drug discovery using cell health microplate assays

Sponsored by: Thermo Fisher Scientific

Specialty

Cell Biology

Molecular Biology

Cancer Research

Cell Culture

Biotechnology

Immunology

Biomarkers

Cancer Therapeutics

Genetics

Drug Discovery

Gene Expression

Biochemistry

Cell Signaling / Transduction

Molecular Diagnostics

Clinical Research

Geography

North America46%

Europe33%

Asia14%

South America5%

Oceania1%

Africa1%

Registration Source

Email Promotions48%

Website Visitors34%

WAVE Newsletter Promotion8%

Client/Sponsor Promotions6%

Social Media Promotions2%

Trending Newsletter Promotion1%

Other1%

Job Title

Research Scientist21%

Student19%

Educator/Faculty11%

Scientist10%

Executive8%

Facility/Department Manager7%

Medical Laboratory Technician5%

Post Doc5%

Medical Doctor/Specialist4%

Lab Management3%

Marketing/Sales3%

Clinical Laboratory Scientist2%

Biologist1%

Organization

Academic Institution41%

Biotech Company10%

Research Institute9%

Life Science Company7%

Hospital5%

Pharmaceutical Company4%

Medical School3%

Clinical Laboratory2%

Medical Center2%

Government2%

Non-Profit Organization2%

Consultant1%

Industrial Company1%

Distribution/sales1%

Other3%


Show Resources
Loading Comments...
Show Resources