SEP 23, 2020 1:30 PM PDT

Keynote Presentation: Starting at the source: tissue extracellular vesicles and Alzheimer's disease

Presented at: Cell Biology 2020
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Associate Professor, Molecular and Comparative Pathobiology, John Hopkins University School of Medicine
    Biography

      Kenneth Witwer is an associate professor of molecular and comparative pathobiology at the Johns Hopkins University School of Medicine and is affiliated with the Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease. The Witwer laboratory studies the roles of extracellular vesicles and extracellular RNA in health and disease, especially in neurodegenerative disease and enveloped virus infection. Dr. Witwer has served as secretary general and executive chair of science and meetings with the International Society for Extracellular Vesicles and is an associate editor at the Journal of Extracellular Vesicles.


    Abstract

    Extracellular vesicles (EVs) are lipid bilayer-delimited pieces of cells that are released from the plasma membrane as "ectosomes" and from the endosomal system as "exosomes." EVs serve both to dispose of toxic materials and to facilitate intercellular communication, so they have many roles to play in disease, including neurodegenerative diseases such as Alzheimer's. Although the long-distance, inter-organ actions of EVs are an exciting topic of research, EVs in solid tissues are most likely to act locally, near the site of release. Our group has adapted a rigorous method for EV separation from tissues. Here, we discuss this method and some of the challenges and opportunities it presents. We then examine molecular profiling of EVs from brain of Alzheimer's disease patients and matched controls and assess possible contributions of APOE genotype. We review the implications of our findings for peripheral biomarkers of disease, using single-particle characterization technologies. All results are shared in the context of the "Minimal Information for Studies of EVs" (MISEV), the consensus document on best practices in the field that was issued by the International Society for Extracellular Vesicles and most recently updated as MISEV2018 (Théry and Witwer, et al, J Extracell Vesicles, 2018). We expect that rigorous and reproducible EV science will continue to develop and contribute to personalized medicine in the coming years.

    Learning Objectives:

    1. Explain the challenges and opportunities in studying extracellular vesicles in tissue

    2. Identify associations between Alzheimer's disease and EV cargo

    3. Introduce rigorous approaches to EV separation and characterization


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