Lung immune cells as drivers of influenza outcomes

Speaker

Abstract

Influenza severity is determined by the interplay between the virus and the host response. Previously, we identified a three-pronged lung gene expression signature that predicted severe influenza outcomes in mice. We hypothesized that this signature resulted from differential activation or infiltration of immune cells in the lungs during mild versus severe influenza infection. In order to build a comprehensive model of lung immune dynamics in response to influenza virus infections, we employed tissue deconvolution and linear regression to model the lung immune cell changes that occurred during mild or severe influenza virus infections. Modeling accurately predicted known macrophage dynamics that occur during influenza infection in vivo, and further predicted a novel population that transcriptionally resembled small serosal macrophages. This macrophage population was predicted to be present in the lungs of animals that recovered from influenza infection and absent from the lungs of those that succumbed. Overall, we provide a multidimensional analysis that delineates how immune cell responses relate to immunopathological consequences of influenza infection. We also demonstrate the utility of bulk tissue gene expression data in generating hypotheses about immune cell dynamics.

Learning Objectives:

1. Understand how different lung immune cell populations impact influenza severity

2. Understand how gene expression data can be used to generate hypotheses about immune cell dynamics


Show Resources
You May Also Like
FEB 15, 2023 7:00 AM PST
FEB 15, 2023 7:00 AM PST
Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
OCT 12, 2022 9:00 AM PDT
C.E. CREDITS
OCT 12, 2022 9:00 AM PDT
Date: October 12, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) The microbiome of every food product is incredibly diverse and can include everything from innocuous background flora...
C.E. CREDITS
Date: October 25, 2022 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CEST) Candida auris is a multi-drug resistant yeast that continues to be a global threat for infection and transmission in h...
SEP 28, 2022 9:00 AM PDT
SEP 28, 2022 9:00 AM PDT
Date: September 28, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) While COVID-19 continues to be the most pressing diagnostic focus for clinical laboratories, HIV remains a serious g...
NOV 16, 2022 7:00 PM PST
C.E. CREDITS
NOV 16, 2022 7:00 PM PST
Date: November 16, 2022 Time: 2:00pm (AEST) Date: November 17, 2022 7:00pm (PST), 10:00pm (EST), 4:00am (CET) The growth in FDA-approved cell and gene therapy products for the treatment of d...
Loading Comments...
Show Resources
Attendees