SEP 27, 2018 7:30 AM PDT

Keynote Presentation: Mapping the Inner World of the Cell

Presented at: Cell Biology 2018
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Professor, Department of Pharmaceutical Chemistry, Biochemistry and Biophysics, University of California, San Francisco
    Biography
      Dr. Bo Huang received his B.S. degree in Chemistry from Peking University, China, in 2001 and Ph. D. degree in Chemistry at Stanford University in 2006. After finishing postdoc work at Harvard University in 2009, he joined UCSF as an Assistant Professor of Pharmaceutical Chemistry, with a joint appointment in the Department of Biochemistry & Biophysics. He was promoted to Associate Professor in 2014 and Professor in 2017. Dr. Huang's research work encompasses the areas of single molecule biophysics, microscopy, and cell biology. Dr. Huang has received many awards, including the GE Healthcare and Science Prize for Young Life Scientists, Searle Scholarship, Packard Fellowship for Science and Engineering, the NIH Director's New Innovator Award, the American Society for Cell Biology Young Life Scientist Award, and UCSF Byers Award for Basic Science.

    Abstract

    Cellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To uncover the underlying organizational principles and their functional relevance, we take microscopy visualization as the primary approach to systematically map the spatial localization, temporal dynamics and activity profiles of proteins and nucleic acids. For this purpose, we have developed a fragment-tagging approach which fluorescently labels target proteins using our engineered split fluorescent proteins. This approach has enabled systematic tag knock-in in cell lines through CRISPR/Cas9-medied gene editing. Our method paves the way for the large-scale generation of endogenously tagged human cell lines for the proteome-wide analysis of protein localization and interaction networks in a native cellular context.
     

    Learning Objectives: 

    1. Methods to labeling endogenous proteins in cells by gene editing
    2. New fluorescent label for cellular proteins


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