JUN 23, 2020 10:00 AM PDT

Mesenchymal stromal or stem cell (MSC) workflow for efficient clinical translation: from automated GMP-compliant cell manufacturing to standardized phenotyping

Sponsored by: Miltenyi Biotec
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract

DATE:  June 23, 2020

TIME:  10:00am PT

 

Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, such as COVID-19 disease. Several clinical trials have been started, and preliminary data hold great promise for clinical use and cell therapy for this severe disease. To facilitate efficient clinical translation, we developed an automated GMP-compliant cell manufacturing procedure using the integrated cell processing platform CliniMACS Prodigy®, which increases the level of process standardization and product safety. In this new adherent cell culture (ACC) system, sample cells are processed in a closed system, including automated density gradient centrifugation in case of human bone marrow sample, feeding, replating, expanding, and harvesting procedures. Clinical applications of MSCs for spine and joint pain needs only small amount stem cells injected locally. However, for systemic illness such as COVID-19, millions of cells are needed for IV inject. Our xeno-free MSC-Brew GMP Medium, following the recommendations of USP <1043> on ancillary materials, enables efficient isolation and expansion of MSCs from different tissue sources including human bone marrow, adipose tissue, and umbilical cord. Together with the ACC system,  over 200 million MSCs can be generated from single donor in just 14 days. Cultured MSCs need to go through strict quality control before any clinical applications. Our new MSC phenotyping kit, including 3 positive and 5 negative MSC markers based on ISCT standard, facilitates standardized and reliable phenotyping of cultured human MSCs from different tissue sources. The high-purity recombinant engineered antibodies (REAfinity™) in the kit ensures high lot-to-lot consistency and reproducible results in flow cytometry.  

 

Learning objectives:

  • Discuss GMP grade serum-free and xeno-free Medium for efficient MSCs expansion from different tissue resources
  • Discuss Automated and closed Adherent Cell Culture (ACC) System for GMP scale MSC production
  • Discuss Standardized and reliable phenotyping of cultured MSCs

 

 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


Show Resources
You May Also Like
JUN 28, 2022 6:45 AM PDT
JUN 28, 2022 6:45 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
APR 28, 2022 8:00 AM PDT
APR 28, 2022 8:00 AM PDT
Date: April 28, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Human pluripotent stem cells (PSCs) and their derivatives hold great potentials in...
APR 26, 2022 7:00 AM PDT
C.E. CREDITS
APR 26, 2022 7:00 AM PDT
Date: April 19, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) High-content (HC) phenotypic profiling approaches are a powerful tool to study the effect of biological, genetic, and ch...
MAR 30, 2022 6:00 AM PDT
MAR 30, 2022 6:00 AM PDT
Targeted NGS has been instrumental in helping the healthcare community deliver on the promise of precision medicine. The Ion Torrent Genexus Integrated Sequencer has enabled targeted genomic...
Loading Comments...
Show Resources