MAY 31, 2018 10:30 AM PDT

Multi-Tissue Experiments in a Scalable and Automation-Compatible Format

Presented at: Lab Automation 2018
Speaker
  • Head of Department, InSphero AG
    Biography
      Olivier Frey leads the Technology and Platforms group at InSphero AG, Switzerland. Before joining InSphero, he was group leader at the Department of Biosystems Science and Engineering of ETH Zurich, Switzerland. In the Bio Engineering Laboratory of Prof. Andreas Hierlemann he was responsible for the development of integrated microfluidic systems for single cell handling and 3D tissue cultures. Included are in particular multi-tissue systems, or so-called "Body-on-a-Chip" configurations based on 3D microtissue spheroids for microtissue culturing, analysis and interaction. Olivier Frey received his Doctoral degree in Micro Engineering from EPF Lausanne, Switzerland, Laboratory of Prof. Nico de Rooij.

    Abstract

    The next step towards more biomimetic in vitro models is the design of multi-organ devices, which allow for communication of different tissue types. Combining physiologically relevant organ models in perfusion systems bears technological challenges and often leads to complicated culturing setups. Complex systems require trained personnel, feature lower reproducibility and make integration into scalable routine processes difficult. The presented multi-tissue platform features microfluidic channels and chambers that were specifically engineered for culturing of microtissue spheroids under physiological flow conditions. The platform has a plate-format, is fabricated completely out of polystyrene and complies with SBS-standard dimensions. Each plate includes 8 parallel channels, each channel contains 10 microtissue compartments. The compartments have minimal dead volume (<2 uL) and the so-called ‘StandingDropPort’ render microtissues individually accessible, so that a robotic pipet tip can be used for parallelized microtissue loading and retrieval. Open media reservoirs are located at both ends of each channel. Perfusion flow is generated through tilting the device back and forth on an automated system inside an incubator. Multiple devices can be operated in parallel, which increases the number of conditions and statistical replicates that can be executed in parallel. The concept allows for on-demand interconnection of up to 10 identical or different microtissues per channel in a very flexible way. With the broad range of available spheroid-based organ-models, a variety of pre-clinical testing applications can be served using the very same platform.
     

    Learning Objectives: 

    1. Understanding the physiological relevance and handling of 3D microtissue spheroids
    2. How to perform multi-tissue experiments in a scalable and reproducible way


    Show Resources
    You May Also Like
    NOV 10, 2020 7:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    NOV 10, 2020 7:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: November 10, 2020 TIME: 7:00am PDT, 10:00am EDT Automation can provide tremendous benefits such as increased pipetting precision and accuracy, productivity, and throughput. Numerous wo...
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    SEP 02, 2020 7:00 AM PDT
    C.E. CREDITS
    SEP 02, 2020 7:00 AM PDT
    DATE: September 2, 2020 TIME: 03:00pm PDT, 6:00pm EDT Spatial omics is an expanding collection of methods to examine biological molecules in their geographical context. By retaining the prec...
    AUG 18, 2020 10:00 AM PDT
    C.E. CREDITS
    AUG 18, 2020 10:00 AM PDT
    DATE: August 18, 2020 TIME: 10:00am PT Get deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the n...
    JUN 23, 2020 10:00 AM PDT
    C.E. CREDITS
    JUN 23, 2020 10:00 AM PDT
    DATE: June 23, 2020 TIME: 10:00am PT Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, s...
    JUN 09, 2020 10:00 AM PDT
    C.E. CREDITS
    JUN 09, 2020 10:00 AM PDT
    DATE: June 9, 2020 TIME: 10:00am PT, 1:00pm ET The presentation will first discuss sepsis as a disease and then explain the importance of performing diagnostic tests in the clinical labora...
    Loading Comments...
    Show Resources
    Attendees
    • See more