NOV 03, 2016 6:00 AM PDT

Mutation Detection Simplified: From Liquid Biopsy in clinical oncology to the detection of Drug Resistance Associated Mutations in virology

Speakers

Abstract

Sanger sequencing and polymerase chain reaction (PCR) methods have been the standard molecular methods in clinical diagnostics for decades.  Next-Generation Sequencing (NGS) technology revolutionized the field of genomics, transcriptomics and metagenomics and is now swiftly becoming a routine method in different areas of clinical diagnostics [1,2].

Vela Diagnostics developed an integrated automated multi-purpose Sentosa® workflow, which consists of:

  1. A customized robotic liquid handling system for nucleic acid extraction, PCR set-up and/ or NGS library preparation (Sentosa® SX101) 
  2. Instruments for real-time PCR or template preparation and deep sequencing
  3. Kits for nucleic acid extraction, real-time PCR-based tests, NGS library preparation assays and reagents for deep sequencing
  4. Flexible and easily customizable assay specific applications
  5. Data analysis and reporting software

The combined automated qPCR & NGS Sentosa® workflow is a reliable and efficient in-vitro diagnostics tool for the detection and/or quantitation of a wide range of bacterial and viral pathogens as well as gene mutations. The unique ability of the Sentosa workflow is to provide complete & relevant information to aid clinical decision-making and patient management. 
 
We’ll look at how this single, rapid workflow may be applied to HIV and HCV, delivering accurate genotyping information and in the detection of drug-resistant mutations.
 
We’ll also see how this workflow is able to deliver actionable oncology results using liquid biopsy samples (cfDNA) with utility in NSCLC, CRC, and BRAF mutation detection.
 


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NOV 03, 2016 6:00 AM PDT

Mutation Detection Simplified: From Liquid Biopsy in clinical oncology to the detection of Drug Resistance Associated Mutations in virology



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