Sanger sequencing and polymerase chain reaction (PCR) methods have been the standard molecular methods in clinical diagnostics for decades. Next-Generation Sequencing (NGS) technology revolutionized the field of genomics, transcriptomics and metagenomics and is now swiftly becoming a routine method in different areas of clinical diagnostics [1,2].
Vela Diagnostics developed an integrated automated multi-purpose Sentosa® workflow, which consists of:
The combined automated qPCR & NGS Sentosa® workflow is a reliable and efficient in-vitro diagnostics tool for the detection and/or quantitation of a wide range of bacterial and viral pathogens as well as gene mutations. The unique ability of the Sentosa workflow is to provide complete & relevant information to aid clinical decision-making and patient management.
We’ll look at how this single, rapid workflow may be applied to HIV and HCV, delivering accurate genotyping information and in the detection of drug-resistant mutations.
We’ll also see how this workflow is able to deliver actionable oncology results using liquid biopsy samples (cfDNA) with utility in NSCLC, CRC, and BRAF mutation detection.