JAN 31, 2019 10:30 AM PST

Overcoming Limitations of Conventional Tag Systems - Strep-Tactin® XT Applications

Presented at: BioProcessing 2019
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Scientist and Team Leader, Cell Cultivation, IBA Life Sciences
    Biography
      Dennis Karthaus received his master's degree in biotechnology from the University of Applied Sciences in Bremerhaven. During his master's study at the department of Pharmaceutical Biotechnology at the Fraunhofer Institute for Toxicology and Experimental Medicine, he worked on the development of a protein purification platform and in cell line development. In 2012, Dennis Karthaus joined IBA GmbH. He is responsible for the mammalian custom protein expression & purification service and the product development in this field.

    Abstract

    The Strep-Tactin®XT:Twin-Strep-tag®-purification system enables protein purification at high yields and purity under physiological conditions. Providing the highest binding affinity among all affinity tag systems, the technology fulfills the demands of mammalian expression systems (e.g. Expi) and is well suited for downstream applications like SPR or the switchSENSE® technology.

    Learning Objectives: 

    1. Purification of Twin-Strep-tag® fusion proteins from Expi293TM and ExpiCHOTM supernatants using Strep-Tactin®XT
    2. Learn how the Twin-Strep-tag®/Strep-Tactin®XT system can simplify kinetic studies using SPR or dynamic Biosensors switchSENSE® technology 



     


    Show Resources
    You May Also Like
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    Starting Soon!
    C.E. CREDITS
    Starting Soon!
    DATE: June 2, 2020 TIME: 9:00am PT, 12:00pm ET Osmolality is a well-established parameter for upstream bioprocessing, formulation, analytical development and QC applications throughout the m...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    JUN 23, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 23, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: June 23, 2020 TIME: 10:00am PT Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, s...
    JUN 09, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: June 9, 2020 TIME: 10:00am PT, 1:00pm ET The presentation will first discuss sepsis as a disease and then explain the importance of performing diagnostic tests in the clinical labora...
    Loading Comments...
    Show Resources
    Attendees
    • See more