Overcoming Limitations of Conventional Tag Systems - Strep-Tactin® XT Applications

The Strep-Tactin®XT:Twin-Strep-tag®-purification system enables protein purification at high yields and purity under physiological conditions. Providing the highest binding affinity among all affinity tag systems, the technology fulfills the demands of mammalian expression systems (e.g. Expi) and is well suited for downstream applications like SPR or the switchSENSE® technology.

Learning Objectives: 

1. Purification of Twin-Strep-tag® fusion proteins from Expi293TM and ExpiCHOTM supernatants using Strep-Tactin®XT
2. Learn how the Twin-Strep-tag®/Strep-Tactin®XT system can simplify kinetic studies using SPR or dynamic Biosensors switchSENSE® technology