JAN 31, 2019 10:30 AM PST

Overcoming Limitations of Conventional Tag Systems - Strep-Tactin® XT Applications

Presented at: BioProcessing 2019
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Scientist and Team Leader, Cell Cultivation, IBA Life Sciences
    Biography
      Dennis Karthaus received his master's degree in biotechnology from the University of Applied Sciences in Bremerhaven. During his master's study at the department of Pharmaceutical Biotechnology at the Fraunhofer Institute for Toxicology and Experimental Medicine, he worked on the development of a protein purification platform and in cell line development. In 2012, Dennis Karthaus joined IBA GmbH. He is responsible for the mammalian custom protein expression & purification service and the product development in this field.

    Abstract

    The Strep-Tactin®XT:Twin-Strep-tag®-purification system enables protein purification at high yields and purity under physiological conditions. Providing the highest binding affinity among all affinity tag systems, the technology fulfills the demands of mammalian expression systems (e.g. Expi) and is well suited for downstream applications like SPR or the switchSENSE® technology.

    Learning Objectives: 

    1. Purification of Twin-Strep-tag® fusion proteins from Expi293TM and ExpiCHOTM supernatants using Strep-Tactin®XT
    2. Learn how the Twin-Strep-tag®/Strep-Tactin®XT system can simplify kinetic studies using SPR or dynamic Biosensors switchSENSE® technology 



     


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