SEP 11, 2019 6:00 AM PDT

Powerful Species-Level Identification and Quantification by Weighted Analysis of All, Rather Than One, 16S Hypervariable Regions

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Director Scientific Collaboration , Department of Genetics, Washington University School of Medicine
    Biography
      Seth Crosby is the Director of Science Collaboration in the Department of Genetics at Washington University. Crosby works with groups within and outside the University to leverage technology to achieve research and clinical goals. He has been at Washington University for 16 years. During that time, he directed the Department of Genetics Microarray Core, which is now part of GTAC and then GTAC, itself, during its first two years. Prior to that he was the Director of Translational Research at the Genome Center (now the McDonnell Genome Institute). Prior to his tenure at Washington University, Dr. Crosby worked in the field of genomics and drug discovery at Abbott Laboratories, Pfizer and Pharmacia.

    Abstract

    MVRSION is a bench and informatic system which employs simultaneous weighted analysis eight of nine 16S hyper variable regions. We use Fluidigm Juno to amplify and index these regions in up to 192 samples per run. These are sequenced and analyzed in parallel, using the MVRSION computational protocol. Both accuracy and economy exceed other 16S methods, such as QIIME.

    Learning Objectives: 

    1. Review of 16S rRNA gene sequencing which allows for microbiome characterization with less sequencing/cost than whole genome sequencing
    2. Method for weighted analysis of almost all the hype rvariable regions


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