Recombination, lineage-specific mutations and the emergence of SARS-CoV-2

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Associate Research Scientist, Columbia University
    Biography

      Ioan Filip is an Associate Research Scientist in Systems Biology, and a member of the Program for Mathematical Genomics at Columbia University. He received his Ph.D. in Mathematics from Columbia in 2016. At the Rabadan Lab, Ioan's work is focused on the genetic basis of the immune response to viral infections, as well as on the role of the immune system in cancer progression and treatment. Ioan is also interested in developing algebraic and topological methods to model both viral and tumor evolution.


    Abstract

    The recent outbreak of SARS-CoV-2 underscores the need for understanding the evolutionary processes that drive the emergence and adaptation of zoonotic viruses in humans. Here, we show that recombination in betacoronaviruses, including human-infecting viruses like SARS-CoV and MERS-CoV, frequently encompasses the Receptor Binding Domain (RBD) in the Spike gene. We find that SARS-CoV and SARS-CoV-2 are involved in an ancestral recombination event affecting the RBD. As a result of this recombination event, SARS-CoV and SARS-CoV-2 share a similar genotype in RBD, including two insertions (positions 432-436 and 460-472), and alleles 427N and 436Y which belong to a helix that interacts with the human ACE2 receptor. We perform reconstruction of ancestral states and protein-binding affinity analyses in order to understand what role this ancestral recombination involving SARS-CoV and SARS-CoV-2 might have played in the emergence of the new pandemic virus. We also analyze the ACE2-binding affinity of lineage-specific mutations in the SARS-CoV-2 RBD.

    Learning Objectives:

    1. Discuss the major drivers of viral evolution

    2. Identify hotspots of recombination in coronaviruses

    3. Present in-silico predictions of virus binding affinity to the host receptor, and explain what these predictions show about the emergence of SARS-CoV-2


    Show Resources
    You May Also Like
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    DEC 02, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    JUL 22, 2020 10:00 AM PDT
    C.E. CREDITS
    JUL 22, 2020 10:00 AM PDT
    DATE: July 23, 2020 TIME: 10:00 am PDT The SARS-CoV-2 pandemic has taken a toll on many sectors of the medical community. As the pandemic took a grip on the laboratory, the need for diagnost...
    JUN 09, 2020 10:00 AM PDT
    C.E. CREDITS
    JUN 09, 2020 10:00 AM PDT
    DATE: June 9, 2020 TIME: 10:00am PT, 1:00pm ET The presentation will first discuss sepsis as a disease and then explain the importance of performing diagnostic tests in the clinical labora...
    AUG 25, 2020 8:00 AM PDT
    C.E. CREDITS
    AUG 25, 2020 8:00 AM PDT
    DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
    Loading Comments...
    Show Resources