Process Development Manager in the Cell and Gene Therapy Group at Thermo Fisher Scientific
BIOGRAPHY
MAY 10, 2017 3:00 PM PDT
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Large scale serum free suspension lentiviral production for cell and gene therapy application
Presented at:
2017 Gibco ExpressionWorld
Speaker
Abstract
Lentiviral vectors have become the center of attention for its use as gene transfer vectors in gene therapy. Here, we have developed a new lentiviral production system for the clinical grade production of Lentiviral vectors on a large-scale serum-free suspension platform. This technology employs a newly developed propriety set of GMP reagents comprising of culture media, suspension cells, transfection reagent and boosting enhancers. With new culture media and media supplement, high density cell growth is optimized for maximum LVVs production. Customized lentivirus transfection reagents ensure highly efficient plasmids delivery. LVVs production is further elevated by the boosting enhancers. Taken together, our innovative system is able to deliver >2-3.0E+08 (TU/mL) functional titer with un-concentrated LVVs. We demonstrate that this system can be linearly adapted to bioreactors and wavebags system for large scale LVVs production without compromising the virus yield, which is ideal for industrial needs of gene therapy.
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MAY 10, 2017 3:00 PM PDT
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Large scale serum free suspension lentiviral production for cell and gene therapy application
Presented at:
2017 Gibco ExpressionWorld
Specialty
Biotechnology
Genetics
Molecular Biology
Cell Biology
Cancer Research
Cell Culture
Virology
Pcr/rt-Pcr/real-Time Pcr
Cancer Diagnostics
Medicine
Molecular Diagnostics
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Oncology
Microbiology
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