DEC 11, 2019 7:00 AM PST

A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Sponsored by: SCIEX
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Scientist in Analytical Research and Development, Pfizer
    Biography
      Ms. Reed received her Bachelor's Degree in Cell and Molecular Biology from Missouri State University. After graduating from college, she joined Wyeth Biopharma in St Louis, working on the Refacto FVIII therapy and gaining ample experiences in QC analyticals.

      After that, she joined the biologics formulation development group at Pfizer in St. Louis. A few years later she transitioned to biologics Analytical Research and Development line, working with a diverse group of analytical technologies, and eventually adopting CE as her main technology focus.

      She has worked with the Sciex CE technology for about 11 years by now, most recently moving into a role of method development for novel biological projects. Her current work is focused on development of methods to evaluate critical quality attributes to be validated in support of clinical trials for gene therapy projects and BLA submissions.

    Abstract
    DATE:   December 11, 2019
    TIME:    7:00am PST, 10:00am EST
     
    An important part of the biopharma industry, Plasmid DNA is used as a gene-delivery vehicle for DNA vaccination and as a key intermediate for processes like viral particle production for gene therapy and ex-vivo protein synthesis. Most plasmid DNA preparations contain several topological variants or isoforms including the supercoiled, open-circular and linear forms of the molecule.
     
    Federal regulations require purity testing for manufactured injectable plasmid products and recommend establishing a release criterion of > 80% supercoiled content. Unfortunately, traditional agarose gel has limitations on quantitation. Capillary electrophoresis with Laser Induced Fluorescence detection (CE-LIF) provides a rapid, sensitive, reproducible and automated method for the quantitative analysis of plasmid DNA isoforms.
     
    In this webinar, we present a method for plasmid analysis by CE-LIF with the use of a common dye. Results obtained with two large plasmids at 7 to 10 kb demonstrate that this method is sensitive and robust, providing baseline resolution of supercoiled, open-circular and linear plasmid isoforms within 20 minutes, and is suitable for testing plasmid purity and monitoring plasmid degradation.
     
    Learning Objectives:
    • Demonstrate fast analysis of plasmid samples with separation time within 20 minutes
    • Identify Baseline resolution of supercoiled, linear and open circular isoforms of a 7 kb and a 10 kb plasmid
    • Illustrate streamlining workflows using commercially available dsDNA 1000 kit
    • Explain automated sample separation and data analysis provide rapid, reproducible and quantitative results. 
     
     
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

    Show Resources
    You May Also Like
    AUG 25, 2020 8:00 AM PDT
    C.E. CREDITS
    AUG 25, 2020 8:00 AM PDT
    DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    OCT 08, 2020 7:00 AM PDT
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    NOV 16, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    NOV 10, 2020 7:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    NOV 10, 2020 7:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: November 10, 2020 TIME: 7:00am PDT, 10:00am EDT Automation can provide tremendous benefits such as increased pipetting precision and accuracy, productivity, and throughput. Numerous wo...
    OCT 29, 2020 6:00 AM PDT
    C.E. CREDITS
    OCT 29, 2020 6:00 AM PDT
    Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
    Loading Comments...
    Show Resources
    Attendees
    • See more