DEC 11, 2019 7:00 AM PST

A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Sponsored by: SCIEX
Speaker

Abstract
DATE:   December 11, 2019
TIME:    7:00am PST, 10:00am EST
 
An important part of the biopharma industry, Plasmid DNA is used as a gene-delivery vehicle for DNA vaccination and as a key intermediate for processes like viral particle production for gene therapy and ex-vivo protein synthesis. Most plasmid DNA preparations contain several topological variants or isoforms including the supercoiled, open-circular and linear forms of the molecule.
 
Federal regulations require purity testing for manufactured injectable plasmid products and recommend establishing a release criterion of > 80% supercoiled content. Unfortunately, traditional agarose gel has limitations on quantitation. Capillary electrophoresis with Laser Induced Fluorescence detection (CE-LIF) provides a rapid, sensitive, reproducible and automated method for the quantitative analysis of plasmid DNA isoforms.
 
In this webinar, we present a method for plasmid analysis by CE-LIF with the use of a common dye. Results obtained with two large plasmids at 7 to 10 kb demonstrate that this method is sensitive and robust, providing baseline resolution of supercoiled, open-circular and linear plasmid isoforms within 20 minutes, and is suitable for testing plasmid purity and monitoring plasmid degradation.
 
Learning Objectives:
  • Demonstrate fast analysis of plasmid samples with separation time within 20 minutes
  • Identify Baseline resolution of supercoiled, linear and open circular isoforms of a 7 kb and a 10 kb plasmid
  • Illustrate streamlining workflows using commercially available dsDNA 1000 kit
  • Explain automated sample separation and data analysis provide rapid, reproducible and quantitative results. 
 
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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DEC 11, 2019 7:00 AM PST

A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Sponsored by: SCIEX


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