DATE: December 11, 2019
TIME: 7:00am PST, 10:00am EST
An important part of the biopharma industry, Plasmid DNA is used as a gene-delivery vehicle for DNA vaccination and as a key intermediate for processes like viral particle production for gene therapy and ex-vivo protein synthesis. Most plasmid DNA preparations contain several topological variants or isoforms including the supercoiled, open-circular and linear forms of the molecule.
Federal regulations require purity testing for manufactured injectable plasmid products and recommend establishing a release criterion of > 80% supercoiled content. Unfortunately, traditional agarose gel has limitations on quantitation. Capillary electrophoresis with Laser Induced Fluorescence detection (CE-LIF) provides a rapid, sensitive, reproducible and automated method for the quantitative analysis of plasmid DNA isoforms.
In this webinar, we present a method for plasmid analysis by CE-LIF with the use of a common dye. Results obtained with two large plasmids at 7 to 10 kb demonstrate that this method is sensitive and robust, providing baseline resolution of supercoiled, open-circular and linear plasmid isoforms within 20 minutes, and is suitable for testing plasmid purity and monitoring plasmid degradation.
Demonstrate fast analysis of plasmid samples with separation time within 20 minutes
Identify Baseline resolution of supercoiled, linear and open circular isoforms of a 7 kb and a 10 kb plasmid
Illustrate streamlining workflows using commercially available dsDNA 1000 kit
Explain automated sample separation and data analysis provide rapid, reproducible and quantitative results.
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LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.