SEP 22, 2015 8:00 AM PDT

WEBINAR: SPR for Aptamer-Based Molecular Interactions in Programmable Materials

Speaker
  • PhD Candidate, Penn State University, Department of Bioengineering
    Biography
      Erin Gaddes is a doctoral candidate in the Biomolecular & Biomimetic Materials Lab in the Pennsylvania State University Department of Biomedical Engineering. She began her studies at the University of Notre Dame, graduating with a BS in Chemical and Biomolecular Engineering in 2011. After graduation, Erin entered graduate studies at the Pennsylvania State University, focusing on synthesizing arrays of artificial cell membranes for the study of molecular interactions. She received her MS degree in Bioengineering in 2013 and continued her studies toward a doctorate degree with Dr. Yong Wang. Erin's doctoral work includes the use of surface-functionalized polyvalent aptamers for the selective attachment of biomolecules, specifically circulating tumor cells. For this work, surface plasmon resonance has been used as an integral technique to probe the interactions between aptamers and their biological targets, as well as the formation of DNA polymers from surface-immobilized initiator molecules.

    Abstract
    Surface plasmon resonance (SPR) is a sensitive, label-free technique that detects mass changes due to biomolecular interactions on a surface. This versatile method has been used to evaluate the specificity and binding kinetics of molecules, ranging from short oligonucleotides to whole cells. In this webinar, we will discuss the use of SPR to investigate interactions between aptamers and their targets, as well as the triggered formation of DNA polymers and polyvalent aptamers. In addition, the use of oligonucleotides for the release of protein drugs will be discussed.


    Who Should Attend
    Researchers and students interested in:
    • Surface plasmon resonance
    • Aptamer-based molecular interactions
    • Surface immobilization and functionalization techniques
    • DNA polymerization via sequential hybridization
    • Affinity characterization
    • Triggered release of drugs and small molecules


    Key Learning Objectives
    What you will learn:
    • Benefits of aptamers as affinity molecules
    • Strategies for SPR examination of aptamer-protein interactions
    • Challenges of evaluating DNA polymerization
    • Surface functionalization of polyvalent ligands
    • Methods and results of DNA-triggered drug delivery


    About Reichert Life Sciences
    Reichert Life Sciences' entry into the design and manufacturing of Surface Plasmon Resonance (SPR) instrumentation began in 1997 with efforts to incorporate bio-sensing technology into Reichert's core critical angle refractometer products. This technology built upon a key Reichert patent (US Patent #4,640,616) that describes a digital reflected light refractometer incorporating a charge-coupled device to sense reflected light intensity over a range of angles covering a refractive index range from 1.33 to 1.52. Reichert has since utilized its applications knowledge to develop a full line of SPR systems that push the limits of detection and sensitivity in label-free interaction analysis with technology that drives research from discovery to development to production. Since the sale of Reichert's first SPR device in 2000, the company's equipment has been used in hundreds of published studies.

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