Standardization of Cell Viability Assays in Primary Cells as a Prerequisite for Novel Bioprocessing Applications

Development of novel types of therapeutics require adaptation of traditional bioprocessing applications to include the use of primary cells, such as stem cells, fibroblasts, astrocytes and neurons. Culture of these cells requires significant optimization at the level of assay development and scalability. Detection of changes in cell viability can have a major impact on yield and cost efficiency of bioprocessing applications. Therefore, the use of cell viability assays during early assay development is critical for scalability and implementation success.  In this presentation, I will discuss the development of a custom lactate dehydrogenase assay for the use in both cell lines and primary cells and highlight its use for optimization of bioprocessing applications. 

Learning Objectives:

1. Critically evaluate the need for cell viability assays in the development of bioprocessing applications 
2. Design and troubleshoot cell viability assays for culture of both cell lines and primary cells