JAN 31, 2019 1:30 PM PST

Standardization of Cell Viability Assays in Primary Cells as a Prerequisite for Novel Bioprocessing Applications

Presented at: BioProcessing 2019
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Assistant Professor of Ophthalmology and Molecular Pharmacology & Therapeutics, Dr. John P. and Therese E. Mulcahy Endowed Professor in Ophthalmology, Loyola University Chicago
    Biography
      Dr. Kaja is the Dr. John P. and Therese E. Mulcahy Endowed Professor in Ophthalmology at Loyola University Chicago, where he holds appointments in the Departments of Ophthalmology and Molecular Pharmacology & Therapeutics. He is the Director of the Visual Neurobiology and Signal Transduction Laboratory, and member of the Burn/Shock Trauma Research Institute and the Neuroscience Division. In addition, Dr. Kaja is co-founder and CEO of K&P Scientific LLC, a Chicagoland-based life sciences consulting firm, and Chief Scientific Officer of Experimentica Ltd., a global preclinical contract research organization specializing in ocular disease models. Dr. Kaja's research interest focuses on in vitro and in vivo methods for ophthalmic drug discovery, with an emphasis on optic neuropathies and ocular surface disorders. Dr. Kaja has authored over 50 peer-reviewed manuscripts, numerous book chapters and is a frequent consultant to the biotech and pharmaceutical industry.

    Abstract

    Development of novel types of therapeutics require adaptation of traditional bioprocessing applications to include the use of primary cells, such as stem cells, fibroblasts, astrocytes and neurons. Culture of these cells requires significant optimization at the level of assay development and scalability. Detection of changes in cell viability can have a major impact on yield and cost efficiency of bioprocessing applications. Therefore, the use of cell viability assays during early assay development is critical for scalability and implementation success.  In this presentation, I will discuss the development of a custom lactate dehydrogenase assay for the use in both cell lines and primary cells and highlight its use for optimization of bioprocessing applications. 

    Learning Objectives:

    1. Critically evaluate the need for cell viability assays in the development of bioprocessing applications 
    2. Design and troubleshoot cell viability assays for culture of both cell lines and primary cells


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