SEP 11, 2019 12:00 PM PDT

Understanding Astrovirus Pathogenesis: Who Knew Viruses Had Toxins?

Speaker

Abstract

immunocompromised populations where the virus can cause systemic and even fatal infections. Indeed, we have shown that astrovirus infections are more prevalent than norovirus in our pediatric oncology population with many patients being long-term shedders. Due to the lack of cell culture systems and animal models for most astrovirus family members, little is known about pathogenesis. Our group demonstrated that the human astroviruses cause intestinal epithelial cells to lose cell-to-cell junctions resulting in increased membrane permeability (i.e. leaky gut). The virus does not have to replicate to induce membrane permeability. The outer viral coat protein, or capsid, is all that was required. To determine if this could also happen in vivo, animal models were orally inoculated with species-specific astrovirus or astrovirus capsid and intestinal permeability and clinical disease was monitored. Like cells in culture, the astrovirus capsid protein alone caused a loss of membrane permeability, relocalization of sodium transporters and diarrhea. Our initial work suggests the virus disrupts the barrier by causing the epithelial cells to change and undergo epithelial to mesenchymal transition.


Learning Objectives:

1. Recognize the importance of astroviruses.

2. Illustrate how a viral toxin could cause diarrhea without causing damage to the intestinal cells.

 


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SEP 11, 2019 12:00 PM PDT

Understanding Astrovirus Pathogenesis: Who Knew Viruses Had Toxins?


Specialty

Infectious Disease

Microbiology

Immunology

Laboratory Testing

Diagnostics

Molecular Biology

Clinical Research

Biology

Animal Research

Dna Sequencing

Biomarkers

Animal Models

Clinical Chemistry

Health

Cancer Research

Geography

North America82%

Asia18%

Registration Source

Website Visitors100%

Job Title

Medical Laboratory Technician56%

Educator/Faculty13%

Research Scientist13%

Medical Doctor/Specialist6%

Animal Care Lab Technician6%

Lab Management6%

Organization

Hospital29%

Academic Institution18%

Clinical Laboratory18%

Medical Center12%

Contract Research Organization (Cro)6%

Manufacturer - Other6%

Research Institute6%

Military6%


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