NOV 08, 2017 06:00 PM PST

Utilization of Stem Cells to Crack Motor Neuron Development and Degeneration

Speakers
  • Assistant Research Fellow, Institute of Molecular Biology, Academia Sinica
    Biography
      The focus of research in my laboratory is to elucidate how neurons establish individual identity in the developing nervous system and why only specific neuron subtypes are vulnerable in the neurodegenerative diseases. We tackle these questions via studying non-coding RNAs and their roles during motor neuron generation and degeneration. My lab employs motor neurons generated from mouse and human embryonic (ES) and induced pluripotent (iPS) stem cells, as well as mouse animal models to investigate motor neuron development and disease. We have developed and generated a series of stem cells and animal models to study functions of microRNA and lncRNA by "gain-of-function" and "loss-od-function" approaches. Besides elucidating the basic molecular mechanisms underlying specification of neuronal diversity during CNS development, I apply the stem cell system to study motor neuron diseases. In particular, I am engaged in the establishment and study of patient specific iPS cell based models of Spinal Muscular Atrophy (SMA), Amyotrophic Lateral Sclerosis (ALS). iPS cell derived motor neurons are used in my lab to perform deep sequencing from healthy and ALS motor neurons and to functionally characterize non-coding RNA pathology in motor neuron. I am also a core member of Neuroscience Program and RNA program in Academia Sinica, which provides strong and solid consortium to give advices and exchange the ideas for our projects. In summary, I have multidisciplinary approaches, from in vitro stem cells to in vivo mouse models, to study motor neuron development and degeneration.

    Abstract:

    Regulatory non coding RNAs, including microRNAs (miRNAs) and long non coding RNAs (lncRNAs), have shown to be essential for animal development and viability, yet dissecting the relevance of individual miRNA or lncRNA has been challenging for a given cell context. In addition, the role of ncRNAs for neurodegeneration is still obscure. To identify ncRNAs participated during motor neuron development and degeneration, we used human embryonic stem cell and motor neuron disease iPSCs as paradigms and robustly harnessed them into different motor neuron subtypes to perform strand specific RNA-seq and small RNA-seq simultaneously. We identified several novel MN signature miRNAs and lncRNAs, and systematically analyzed their functions by generating knockout mice. I will present the current progress of the characterization of these MN-ncRNAs by gain-of-function and loss-of-function studies. Collectively, these results will provide critical information for ncRNAs function and will fill in the information on how ncRNAs mediate MN development in vivo.


    Show Resources
    You May Also Like
    MAY 16, 2019 04:00 PM CEST
    C.E. CREDITS
    MAY 16, 2019 04:00 PM CEST
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    JUN 05, 2019 05:00 PM CEST
    C.E. CREDITS
    JUN 05, 2019 05:00 PM CEST
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    NOV 18, 2019 08:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    NOV 18, 2019 08:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: November 18, 2019TIME: 8:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    JUN 19, 2019 10:00 AM PDT
    JUN 19, 2019 10:00 AM PDT
    DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
    Loading Comments...
    Show Resources