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    Cell function

    Cells carry out specialized functions throughout the body and each cell type functions distinctly from other cell types. Some cellular functions include transporting nutrients throughout the body and producing energy. One specialized cell is muscle cells which function to create a force that moves parts of the body. Another example are bone cells, whose functions include protecting internal organs from damage and storing minerals such as calcium.

    Webinars

    • JUL 19, 2022 | 8:00 AM
      Getting started in Cryo-EM
      Getting started in Cryo-EM
      Date: July 19, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Cryo-EM is rapidly becoming the method of choice for structural biologists and biochemists around the world, helping thes...
      Speaker: Melissa Chambers , Natalia de Val
      Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
      Sponsored By: Thermo Fisher Scientific
      9
      JUL 14, 2022 | 8:00 AM
      Imaging long-range neuronal connections in the mouse brain by light sheet fluorescence expansion microscopy
      Imaging long-range neuronal connections in the mouse brain by...
      Date: July 14, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Sensory perception is modulated in a top-down fashion by higher brain regions to regulate behavioral responses. In olfact...
      Speaker: Prof. Ulrich Kubitscheck , Dr. Martin Schwarz , Juan Rodríguez
      Sponsored By: Miltenyi Biotec
      13
      JUL 12, 2022 | 8:00 AM
      Solutions for dendritic cell research
      Solutions for dendritic cell research
      Date: July 12, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CEST) Dendritic cells (DCs) are a heterogeneous population of myeloid immune cells that act as peripheral sentinels specializin...
      Speaker: Dr. Nizar Yousif
      Sponsored By: Miltenyi Biotec
      15
      JUN 29, 2022 | 7:00 AM
      Insights into Structure, Dynamics, and Function of Endocytic Vesicles on the STELLARIS Confocal Platform
      Insights into Structure, Dynamics, and Function of Endocytic Vesicles...
      Date: June 29, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) Imaging live cells is key to reveal their inner workings, dynamics, and function. Current technological advances in confo...
      Speaker: Julia Roberti, Ph.D.
      Sponsored By: Leica Microsystems
      5
      JUN 21, 2022 | 11:00 AM
      Evaluating Monoclonal Gammopathy of Undetermiend Significance (MGUS) with Free Light Chain Assays in Clinical Practice
      Evaluating Monoclonal Gammopathy of Undetermiend Significance...
      Date: June 21, 2022 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEST) Multiple myeloma is a cancer of the plasma cell. Plasma cells are white blood cells that make antibodies that help to pro...
      Speaker: Dr. John Mitsios
      Sponsored By: Siemens Healthineers
      8
      JUN 21, 2022 | 10:00 AM
      Delivering Single-Molecule Proteomics at Scale using Protein Identification by Short epitope Mapping
      Delivering Single-Molecule Proteomics at Scale using Protein Identification...
      Date: June 21, 2022 Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CEST) The proteome is perhaps the most dynamic and valuable source of functional biological insight. Here, we introduce a novel...
      Speaker: Parag Mallick, Ph.D.
      Sponsored By: Nautilus Biotechnology
      9
      JUN 16, 2022 | 8:00 AM
      Scientists Empowering Scientists: About Retinal Electrophysiology, Combining Light Beams and Dealing with Noise
      Scientists Empowering Scientists: About Retinal Electrophysiology,...
      Date: June 16, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Many researchers combine electrophysiological recordings with imaging applications, where a light source with high light...
      Speaker: Telly Galiatsatos , Fatima Abbas, PhD , Jan Dolzer, PhD
      Sponsored By: Sutter Instrument
      7
      JUN 09, 2022 | 8:00 AM
      Revealing the role of Mononuclear phagocytes in human pregnancy via automated high purity isolation
      Revealing the role of Mononuclear phagocytes in human pregnancy...
      Date: June 09, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) It is known that immune responses of pregnant women undergo recognized changes that ensure the mother does not reject the...
      Speaker: Prof. Cathy Thornton, PhD , Salvador Vento Asturias, PhD
      Sponsored By: Miltenyi Biotec
      9
      JUN 01, 2022 | 10:00 AM
      AI Microscopy Symposium, 7th edition
      AI Microscopy Symposium, 7th edition
      Date: June 1, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) The AI Microscopy Symposium, offers an unique forum for presenting and discussing the latest AI-based technologies and too...
      Speaker: Yicong Wu , Vijay Rajagopal , Afshin Khadangi , Parmida Ghahremani
      Sponsored By: Leica Microsystems
      15
      JUN 01, 2022 | 9:00 AM
      The Art and Science of Hematology Morphology: Platelet Morphology
      The Art and Science of Hematology Morphology: Platelet Morphology
      Date: June 01, 2022 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CEST) The enumeration and analysis of blood cells with automated haematology analysers have significantly improved in recent ye...
      Speaker: Daniel Pelling, CSci, FIBMS, DFM
      Sponsored By: Abbott
      56
      MAY 25, 2022 | 10:00 AM
      A digital PCR-based protocol to detect and quantify RNA editing events at hotspots
      A digital PCR-based protocol to detect and quantify RNA editing...
      Date: May 25, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. P...
      Speaker: Rémi Buisson
      Sponsored By: Thermo Fisher Scientific
      22
      MAY 18, 2022 | 12:00 PM
      Panel Presentation: Automating High-throughput Luminex Assays for Multiplex Gene and Protein Analysis
      Panel Presentation: Automating High-throughput Luminex Assays...
      Invitrogen ProcartaPlex and QuantiGene Plex assays offer the unique ability of a multi-omics high-throughput approach by utilizing the Luminex platform. Genomic and proteomic workflows can b...
      Speaker: Stefan Jellbauer, PhD , Bani Suri, PhD
      Presented at: Laboratory Automation Virtual Event Series 2022
      Sponsored By: Luminex - A DiaSorin Company
      2
      MAY 18, 2022 | 5:30 AM
      Measuring Biophysical Properties of Single Cells and Particles with High Precision
      Measuring Biophysical Properties of Single Cells and Particles...
      This talk will focus on novel microfluidic approaches for measuring physical properties of particles in the 10 nm to 10 m range and single cells, with particular focus on high precision mea...
      Speaker: Scott Manalis
      Presented at: Laboratory Automation Virtual Event Series 2022
      1
      MAY 17, 2022 | 8:00 AM
      Novel tools for cell line engineering with CRISPR-Cas9
      Novel tools for cell line engineering with CRISPR-Cas9
      Date: May 17, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) The CRISPR-Cas9 system provides researchers a powerful tool for manipulation of cells to create disease models, reporter c...
      Speaker: Louise Baskin, M.S. Molecular Biology and Genetics
      Sponsored By: Thermo Fisher Scientific
      13
      MAY 12, 2022 | 9:00 AM
      Implementing shallow screening strategies to accelerate microbial strain improvement
      Implementing shallow screening strategies to accelerate microbial...
      Date: May 12, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CET) The ability to move rapidly through the Design-Generate-Test-Learn (DGTL) cycle is essential for effective forward engineer...
      Speaker: Eileen Spindler
      Sponsored By: Inscripta
      7
      MAY 04, 2022 | 5:30 AM
      Neuropathology of SARS-CoV-2 Infection in Aged Non-human Primates
      Neuropathology of SARS-CoV-2 Infection in Aged Non-human Primates
      Neurological manifestations, including headache, altered mental status, and stroke, are a major complication of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and cor...
      Speaker: Tracy Fischer, PhD
      Presented at: Coronavirus Series
      2
      MAY 04, 2022 | 5:30 AM
      Epistatic Interactions Among SARS-CoV-2 Spike Amino Acids Required for Viability of Variants of Concern
      Epistatic Interactions Among SARS-CoV-2 Spike Amino Acids Required...
      During the first year of the SARS-CoV-2 pandemic, international monitoring of SARS-CoV-2 genomic RNA revealed that the virus accumulated roughly 2 mutations per month. Nonetheless, one nonsy...
      Speaker: Jeremy Luban, MD
      Presented at: Coronavirus Series
      2
      APR 26, 2022 | 7:00 AM
      High-Content Phenotypic Screening - Answering Questions You Haven't Even Asked Yet
      High-Content Phenotypic Screening - Answering Questions You Haven't...
      Date: April 19, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) High-content (HC) phenotypic profiling approaches are a powerful tool to study the effect of biological, genetic, and ch...
      Speaker: Dr. Angeline Lim , Dr. Victor Wong
      Sponsored By: Molecular Devices
      5
      APR 21, 2022 | 8:00 AM
      Upgrade your B cell culture
      Upgrade your B cell culture
      Date: April 21, 2022 Time: 8:00am (PDT), 11:00am (EDT), 6:00pm (CEST) Due to the low frequency of B cells in human peripheral blood, it can be difficult to obtain sufficient numbers of B cel...
      Speaker: Dr. Susanne Bethke , Dr. Henriette Zimmermann
      Sponsored By: Miltenyi Biotec
      8
      MAR 31, 2022 | 6:00 AM
      CytoFLEX SRT: The CytoFLEX That Sorts
      CytoFLEX SRT: The CytoFLEX That Sorts
      Date: March 31, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The CytoFLEX SRT Is a compact cell sorter capable of handling the variety...
      Speaker: John Tigges
      Sponsored By: Beckman Coulter
      7
      MAR 24, 2022 | 8:00 AM
      T cell separation: Fine-tuning your T cell research workflow step by step
      T cell separation: Fine-tuning your T cell research workflow step...
      Date: March 24, 2021 Time: 08:00am (PDT), 11:00pm (EDT) Simply enough, we want to treat our cells well. Afterall, we want them to tell us their stories in basic research or work for us in th...
      Speaker: Marcello Stein, PhD.
      Sponsored By: Miltenyi Biotec
      6
      MAR 10, 2022 | 11:55 PM
      Characterizing Pathology-Inducing Protein Aggregates as Tools for Alzheimer's and Parkinson's Disease Models and Drug Discovery
      Characterizing Pathology-Inducing Protein Aggregates as Tools...
      Alzheimer’s and Parkinson’s diseases (AD, PD) are the two most common neurodegenerative disorders. Despite their debilitating effects and increasing prevalence in our society, no...
      Speaker: Jacob McPhail, PhD
      Presented at: Neuroscience Virtual Event Series 2022
      2 1
      MAR 10, 2022 | 11:55 PM
      Higher Resolution Electrodes Improve Our Understanding Human Brain, Cognition, and Neurological Pathologies
      Higher Resolution Electrodes Improve Our Understanding Human Brain,...
      Neuroscience is experiencing an exciting era of integrating novel materials and tools to record neural activity made possible through significant advances in materials science, electrical en...
      Speaker: Angelique C. Paulk, PhD
      Presented at: Neuroscience Virtual Event Series 2022
      1
      MAR 10, 2022 | 7:30 AM
      Spatial Transcriptomic Profiling of Spiny Projection Neurons in the Mouse Striatum
      Spatial Transcriptomic Profiling of Spiny Projection Neurons in...
      NanoString has started an ambitious project called the "Spatial Organ Atlas." The goal of this initiative is to map the architecture of tissues with spatially resolved whole transc...
      Speaker: Alison VanSchoiack, PhD
      Presented at: Neuroscience Virtual Event Series 2022
      Sponsored By: NanoString Technologies
      3
    JUL 19, 2022 | 8:00 AM
    Getting started in Cryo-EM
    Getting started in Cryo-EM
    Date: July 19, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Cryo-EM is rapidly becoming the method of choice for structural biologists and biochemists around the world, helping thes...
    Speaker: Melissa Chambers , Natalia de Val
    Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
    Sponsored By: Thermo Fisher Scientific
    9
    JUL 14, 2022 | 8:00 AM
    Imaging long-range neuronal connections in the mouse brain by light sheet fluorescence expansion microscopy
    Imaging long-range neuronal connections in the mouse brain by...
    Date: July 14, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Sensory perception is modulated in a top-down fashion by higher brain regions to regulate behavioral responses. In olfact...
    Speaker: Prof. Ulrich Kubitscheck , Dr. Martin Schwarz , Juan Rodríguez
    Sponsored By: Miltenyi Biotec
    13
    JUL 12, 2022 | 8:00 AM
    Solutions for dendritic cell research
    Solutions for dendritic cell research
    Date: July 12, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CEST) Dendritic cells (DCs) are a heterogeneous population of myeloid immune cells that act as peripheral sentinels specializin...
    Speaker: Dr. Nizar Yousif
    Sponsored By: Miltenyi Biotec
    15
    JUN 29, 2022 | 7:00 AM
    Insights into Structure, Dynamics, and Function of Endocytic Vesicles on the STELLARIS Confocal Platform
    Insights into Structure, Dynamics, and Function of Endocytic Vesicles...
    Date: June 29, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) Imaging live cells is key to reveal their inner workings, dynamics, and function. Current technological advances in confo...
    Speaker: Julia Roberti, Ph.D.
    Sponsored By: Leica Microsystems
    5
    JUN 21, 2022 | 11:00 AM
    Evaluating Monoclonal Gammopathy of Undetermiend Significance (MGUS) with Free Light Chain Assays in Clinical Practice
    Evaluating Monoclonal Gammopathy of Undetermiend Significance...
    Date: June 21, 2022 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEST) Multiple myeloma is a cancer of the plasma cell. Plasma cells are white blood cells that make antibodies that help to pro...
    Speaker: Dr. John Mitsios
    Sponsored By: Siemens Healthineers
    8
     
     
  • JUN 21, 2022 | 10:00 AM
    Delivering Single-Molecule Proteomics at Scale using Protein Identification by Short epitope Mapping
    Delivering Single-Molecule Proteomics at Scale using Protein Identification...
    Date: June 21, 2022 Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CEST) The proteome is perhaps the most dynamic and valuable source of functional biological insight. Here, we introduce a novel...
    Speaker: Parag Mallick, Ph.D.
    Sponsored By: Nautilus Biotechnology
    9
    JUN 16, 2022 | 8:00 AM
    Scientists Empowering Scientists: About Retinal Electrophysiology, Combining Light Beams and Dealing with Noise
    Scientists Empowering Scientists: About Retinal Electrophysiology,...
    Date: June 16, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Many researchers combine electrophysiological recordings with imaging applications, where a light source with high light...
    Speaker: Telly Galiatsatos , Fatima Abbas, PhD , Jan Dolzer, PhD
    Sponsored By: Sutter Instrument
    7
    JUN 09, 2022 | 8:00 AM
    Revealing the role of Mononuclear phagocytes in human pregnancy via automated high purity isolation
    Revealing the role of Mononuclear phagocytes in human pregnancy...
    Date: June 09, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) It is known that immune responses of pregnant women undergo recognized changes that ensure the mother does not reject the...
    Speaker: Prof. Cathy Thornton, PhD , Salvador Vento Asturias, PhD
    Sponsored By: Miltenyi Biotec
    9
    JUN 01, 2022 | 10:00 AM
    AI Microscopy Symposium, 7th edition
    AI Microscopy Symposium, 7th edition
    Date: June 1, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) The AI Microscopy Symposium, offers an unique forum for presenting and discussing the latest AI-based technologies and too...
    Speaker: Yicong Wu , Vijay Rajagopal , Afshin Khadangi , Parmida Ghahremani
    Sponsored By: Leica Microsystems
    15
    JUN 01, 2022 | 9:00 AM
    The Art and Science of Hematology Morphology: Platelet Morphology
    The Art and Science of Hematology Morphology: Platelet Morphology
    Date: June 01, 2022 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CEST) The enumeration and analysis of blood cells with automated haematology analysers have significantly improved in recent ye...
    Speaker: Daniel Pelling, CSci, FIBMS, DFM
    Sponsored By: Abbott
    56
     
     
  • MAY 25, 2022 | 10:00 AM
    A digital PCR-based protocol to detect and quantify RNA editing events at hotspots
    A digital PCR-based protocol to detect and quantify RNA editing...
    Date: May 25, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CEST) Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. P...
    Speaker: Rémi Buisson
    Sponsored By: Thermo Fisher Scientific
    22
    MAY 18, 2022 | 12:00 PM
    Panel Presentation: Automating High-throughput Luminex Assays for Multiplex Gene and Protein Analysis
    Panel Presentation: Automating High-throughput Luminex Assays...
    Invitrogen ProcartaPlex and QuantiGene Plex assays offer the unique ability of a multi-omics high-throughput approach by utilizing the Luminex platform. Genomic and proteomic workflows can b...
    Speaker: Stefan Jellbauer, PhD , Bani Suri, PhD
    Presented at: Laboratory Automation Virtual Event Series 2022
    Sponsored By: Luminex - A DiaSorin Company
    2
    MAY 18, 2022 | 5:30 AM
    Measuring Biophysical Properties of Single Cells and Particles with High Precision
    Measuring Biophysical Properties of Single Cells and Particles...
    This talk will focus on novel microfluidic approaches for measuring physical properties of particles in the 10 nm to 10 m range and single cells, with particular focus on high precision mea...
    Speaker: Scott Manalis
    Presented at: Laboratory Automation Virtual Event Series 2022
    1
    MAY 17, 2022 | 8:00 AM
    Novel tools for cell line engineering with CRISPR-Cas9
    Novel tools for cell line engineering with CRISPR-Cas9
    Date: May 17, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) The CRISPR-Cas9 system provides researchers a powerful tool for manipulation of cells to create disease models, reporter c...
    Speaker: Louise Baskin, M.S. Molecular Biology and Genetics
    Sponsored By: Thermo Fisher Scientific
    13
    MAY 12, 2022 | 9:00 AM
    Implementing shallow screening strategies to accelerate microbial strain improvement
    Implementing shallow screening strategies to accelerate microbial...
    Date: May 12, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CET) The ability to move rapidly through the Design-Generate-Test-Learn (DGTL) cycle is essential for effective forward engineer...
    Speaker: Eileen Spindler
    Sponsored By: Inscripta
    7
     
     
  • MAY 04, 2022 | 5:30 AM
    Neuropathology of SARS-CoV-2 Infection in Aged Non-human Primates
    Neuropathology of SARS-CoV-2 Infection in Aged Non-human Primates
    Neurological manifestations, including headache, altered mental status, and stroke, are a major complication of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and cor...
    Speaker: Tracy Fischer, PhD
    Presented at: Coronavirus Series
    2
    MAY 04, 2022 | 5:30 AM
    Epistatic Interactions Among SARS-CoV-2 Spike Amino Acids Required for Viability of Variants of Concern
    Epistatic Interactions Among SARS-CoV-2 Spike Amino Acids Required...
    During the first year of the SARS-CoV-2 pandemic, international monitoring of SARS-CoV-2 genomic RNA revealed that the virus accumulated roughly 2 mutations per month. Nonetheless, one nonsy...
    Speaker: Jeremy Luban, MD
    Presented at: Coronavirus Series
    2
    APR 26, 2022 | 7:00 AM
    High-Content Phenotypic Screening - Answering Questions You Haven't Even Asked Yet
    High-Content Phenotypic Screening - Answering Questions You Haven't...
    Date: April 19, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) High-content (HC) phenotypic profiling approaches are a powerful tool to study the effect of biological, genetic, and ch...
    Speaker: Dr. Angeline Lim , Dr. Victor Wong
    Sponsored By: Molecular Devices
    5
    APR 21, 2022 | 8:00 AM
    Upgrade your B cell culture
    Upgrade your B cell culture
    Date: April 21, 2022 Time: 8:00am (PDT), 11:00am (EDT), 6:00pm (CEST) Due to the low frequency of B cells in human peripheral blood, it can be difficult to obtain sufficient numbers of B cel...
    Speaker: Dr. Susanne Bethke , Dr. Henriette Zimmermann
    Sponsored By: Miltenyi Biotec
    8
    MAR 31, 2022 | 6:00 AM
    CytoFLEX SRT: The CytoFLEX That Sorts
    CytoFLEX SRT: The CytoFLEX That Sorts
    Date: March 31, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The CytoFLEX SRT Is a compact cell sorter capable of handling the variety...
    Speaker: John Tigges
    Sponsored By: Beckman Coulter
    7
     
     
  • MAR 24, 2022 | 8:00 AM
    T cell separation: Fine-tuning your T cell research workflow step by step
    T cell separation: Fine-tuning your T cell research workflow step...
    Date: March 24, 2021 Time: 08:00am (PDT), 11:00pm (EDT) Simply enough, we want to treat our cells well. Afterall, we want them to tell us their stories in basic research or work for us in th...
    Speaker: Marcello Stein, PhD.
    Sponsored By: Miltenyi Biotec
    6
    MAR 10, 2022 | 11:55 PM
    Characterizing Pathology-Inducing Protein Aggregates as Tools for Alzheimer's and Parkinson's Disease Models and Drug Discovery
    Characterizing Pathology-Inducing Protein Aggregates as Tools...
    Alzheimer’s and Parkinson’s diseases (AD, PD) are the two most common neurodegenerative disorders. Despite their debilitating effects and increasing prevalence in our society, no...
    Speaker: Jacob McPhail, PhD
    Presented at: Neuroscience Virtual Event Series 2022
    2 1
    MAR 10, 2022 | 11:55 PM
    Higher Resolution Electrodes Improve Our Understanding Human Brain, Cognition, and Neurological Pathologies
    Higher Resolution Electrodes Improve Our Understanding Human Brain,...
    Neuroscience is experiencing an exciting era of integrating novel materials and tools to record neural activity made possible through significant advances in materials science, electrical en...
    Speaker: Angelique C. Paulk, PhD
    Presented at: Neuroscience Virtual Event Series 2022
    1
    MAR 10, 2022 | 7:30 AM
    Spatial Transcriptomic Profiling of Spiny Projection Neurons in the Mouse Striatum
    Spatial Transcriptomic Profiling of Spiny Projection Neurons in...
    NanoString has started an ambitious project called the "Spatial Organ Atlas." The goal of this initiative is to map the architecture of tissues with spatially resolved whole transc...
    Speaker: Alison VanSchoiack, PhD
    Presented at: Neuroscience Virtual Event Series 2022
    Sponsored By: NanoString Technologies
    3
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