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Protein Isolation

Protein Isolation: The initial step in protein isolation from its source is to physically or chemically disrupt the biological tissue or organism in order to release the protein into the extract. In some cases, the organism secretes the protein, and cell disruption is unnecessary. The protein is usually produced in dilute concentration so it is advantageous if the initial isolation also results in the concentration of the protein. An important feature in any isolation procedure is product yield with the goal being to extract as much of the desired protein as possible in a minimum amount of time.

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