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    Translational Oncology

    Translational Oncology involves moving scientific discoveries made in a laboratory setting into clinical applications that enhance treatment efficacy and outcomes for cancer patients. Translational Oncology approaches include pre-clinical research, which can be done in an in vivo, in vitro, or ex vivo setting, before validating the laboratory findings with a clinical trial.

    Webinars

    • MAY 19, 2020 | 10:00 AM
      Applications in Biomarker Discovery and CAR T Therapy Using High-plex RNA & Protein Spatial Profiling on FFPE Tissue
      Applications in Biomarker Discovery and CAR T Therapy Using High-plex...
      The GeoMx Digital Spatial Profiling technology enables high-plex RNA and protein profiling of any region within any tissue type. This workshop will highlight the latest developments toward e...
      Speaker: PLENARY: Carl June, MD
      Presented at: Advancing Science - A Virtual Oncology Conference
      1
      MAY 14, 2020 | 7:05 PM
      Haemato-Oncology Applications of BCR Immune Repertoire Sequencing
      Haemato-Oncology Applications of BCR Immune Repertoire Sequencing
      B-cell lymphomas and leukemias originate from the malignant transformation and proliferation of one or a small number of B cells. Since each B-cell expresses distinct B-cell receptors (BCRs)...
      Speaker: Timothy Looney, PhD
      Presented at: OncomineWorld 2022: A Virtual NGS Education Meeting
      8
      MAY 14, 2020 | 7:05 PM
      Haemato-Oncology Applications of BCR Immune Repertoire Sequencing
      Haemato-Oncology Applications of BCR Immune Repertoire Sequencing
      B-cell lymphomas and leukemias originate from the malignant transformation and proliferation of one or a small number of B cells. Since each B-cell expresses distinct B-cell receptors (BCRs)...
      Speaker: Timothy Looney, PhD
      Presented at: OncomineWorld Autumn: A virtual NGS education meeting
      1
      MAY 14, 2020 | 3:00 PM
      Analysis of Solid Tumor Genomic Landscape in Liquid Biopsy
      Analysis of Solid Tumor Genomic Landscape in Liquid Biopsy
      Increasing evidence suggests that targeted sequencing of cell free DNA (cfDNA) can provide a comprehensive molecular portrait of solid tumors. However, the concordance between tissue and cfD...
      Speaker: Nicola Normanno, MD
      Presented at: OncomineWorld 2022: A Virtual NGS Education Meeting
      8
      MAY 14, 2020 | 3:00 PM
      Analysis of Solid Tumor Genomic Landscape in Liquid Biopsy
      Analysis of Solid Tumor Genomic Landscape in Liquid Biopsy
      Increasing evidence suggests that targeted sequencing of cell free DNA (cfDNA) can provide a comprehensive molecular portrait of solid tumors. However, the concordance between tissue and cfD...
      Speaker: Nicola Normanno, MD
      Presented at: OncomineWorld Autumn: A virtual NGS education meeting
      1
      MAY 12, 2020 | 9:00 AM
      Analytical Quality Control for the Pharmaceutical Industry
      Analytical Quality Control for the Pharmaceutical Industry
      NEW DATE: Date: May 12, 2020 Time: 9:00am PDT, 12:00pm EDT...
      Speaker: Joy McElroy
      Sponsored By: Sartorius
      29 9
      MAY 08, 2020 | 8:00 AM
      Development of a novel molecular MSI assay with an expanded panel and automated MSI status calling
      Development of a novel molecular MSI assay with an expanded panel...
      DATE: May 8, 2020 TIME: 8:00am PT, 11:00am ET Microsatellite instability (MSI) is the condition of genetic hypermutability that results from impaired DNA mismatch repair (MMR). Studies indic...
      Speaker: Charles Baudo, Ph.D.
      Sponsored By: Thermo Fisher Scientific - Applied Biosystems
      8 2
      MAY 07, 2020 | 8:00 AM
      When Cell Growth is Critical: Evaluating COâ‚‚ Incubator Parameters for Cell Therapy
      When Cell Growth is Critical: Evaluating COâ‚‚ Incubator Parameters...
      DATE: May 7, 2020 TIME: 8:00AM PDT, 11:00AM EDT With the recent explosion of cell therapy, we know more about conditions affecting cell growth than ever before. Your CO2 incubator should not...
      Speaker: Mary Kay Bates, M.S.
      Sponsored By: Thermo Fisher Scientific
      15 2
      APR 29, 2020 | 6:00 AM
      High-Throughput assessment of ChIP-grade capability with Luminex xMAP technology
      High-Throughput assessment of ChIP-grade capability with Luminex...
      Histone post-translational modifications (PTMs) play pivotal roles in chromatin dynamics and function, with alterations in the healthy profile associated with diverse human pathologies. The...
      Speaker: Michael-Christopher Keogh, PhD
      Presented at: xMAP® Connect Virtual Conference (On-Demand)
      1
      APR 29, 2020 | 6:00 AM
      A Basic FGF Mimetic Biobetter for Mitigation of Acute Gastrointestinal Radiation Syndrome
      A Basic FGF Mimetic Biobetter for Mitigation of Acute Gastrointestinal...
      In the event of a radiological or nuclear disaster, there will be a need for therapies that can mitigate the acute hematopoietic and gastrointestinal (GI) radiation syndromes expected to dev...
      Speaker: Steven Swarts, PhD
      Presented at: xMAP® Connect Virtual Conference (On-Demand)
      1
      APR 24, 2020 | 8:00 AM
      The International Council for Standardisation in Hematology (ICSH) recommendations for Hemostasis Critical Values, Tests, and Reporting - a stepwise description for the committee's decisions
      The International Council for Standardisation in Hematology (ICSH)...
      The International Council for Standardization in Haematology (ICSH) is a nonprofit organization dedicated to providing open-access guidance documents to enhance the quality of laboratory tes...
      Speaker: Bob Gosselin
      Sponsored By: Diagnostica Stago, Inc.
      10
      APR 22, 2020 | 8:00 AM
      C.E. CREDITS
      The NEW Coag Value Playbook...Embrace the Power of Local Testing
      The NEW Coag Value Playbook...Embrace the Power of Local Testing
      Discover strategies for labs to optimize coagulation testing, improve patient outcomes, and strengthen their role in clinical care teams....
      Speaker: Eleanor Herriman, MD, MBA , Oksana Volod, MD
      Presented at: Stago EdVantage Virtual University
      Sponsored By: Diagnostica Stago, Inc.
      7
      APR 20, 2020 | 8:00 AM
      C.E. CREDITS
      Emergency patient management using coagulation assays
      Emergency patient management using coagulation assays
      Explore emergency hemostasis testing approaches, from rapid lab assays to viscoelastic methods, for managing bleeding patients....
      Speaker: Wayne Chandler, MD
      Presented at: Stago EdVantage Virtual University
      Sponsored By: Diagnostica Stago, Inc.
      17 5
      APR 16, 2020 | 9:00 AM
      Visualize more biomarkers and extract more insights: Moving from single marker IHC to multiplex IF
      Visualize more biomarkers and extract more insights: Moving from...
      DATE: April 16, 2020 TIME: 9:00am PT, 12:00pm ET There is an increasing need to evaluate and understand unique phenotypes and mechanisms of action within the tumor microenvironment. Existing...
      Speaker: Scott Lawrence (M.S., HT(ASCP)) [C] , Katir Patel, PhD
      Sponsored By: Ultivue
      5
      APR 14, 2020 | 9:00 AM
      Gibco Cell Culture Heroes: Work From Home Edition
      Gibco Cell Culture Heroes: Work From Home Edition
      DATE: April 14, 2020 TIME: 9:00am PDT, 12:00pm EDT Besides being Gibco Cell Culture Heroes, what do Vivek, Sandra, Daisy, Ameet and Kristine all have in common? They are all stuck at home fo...
      Speaker: Kristine Wadosky, PhD , Ameet Chimote, PhD , Elizabeth Delery, PhD , Daisy Shu, B.Optom, PhD , Vivek Kamat, PhD , Sandra Hammer, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      12 1
      APR 02, 2020 | 2:30 PM
      COVID-19 Total Solutions for Detection and Surveillance
      COVID-19 Total Solutions for Detection and Surveillance
      BGI group has been at the forefront in the fight against covid-19. Starting from building a lab in Wuhan, sequencing first 2019-nCoV virus genome and offering RT-PCR and sequencing based det...
      Speaker: Subodh Nimkar , Pan Zhang, PhD
      Presented at: Coronavirus Series
      Sponsored By: BGI Americas, MGI Americas
      2
      APR 02, 2020 | 6:00 AM
      A Multi-Omic Approach to Detection and Characterization of Viral Pathogens and Their Impact on the Immune System
      A Multi-Omic Approach to Detection and Characterization of Viral...
      A growing body of researchers around the world is working to mount an aggressive and sustained response to the COVID-19 pandemic. By building on past successes and harnessing insights from c...
      Speaker: Bill Hunt, MS , Andrew Quong, PhD
      Presented at: Coronavirus Series
      Sponsored By: Standard BioTools
      2 1
      MAR 31, 2020 | 9:00 AM
      Impact of liquid biopsy in advanced HER2+ breast cancer tumors: a follow-up study
      Impact of liquid biopsy in advanced HER2+ breast cancer tumors:...
      DATE: March 31, 2020 TIME: 9:00am PT, 12:00pm ET HER2-positive breast cancer is a type of breast cancer caused by over-expression of a protein called human epidermal growth factor receptor 2...
      Speaker: Matteo Allegretti, PhD
      Sponsored By: Thermo Fisher Scientific - Ion Torrent, Thermo Fisher Scientific - Applied Biosystems
      7
      FEB 26, 2020 | 12:00 PM
      Characterizing the role of B cells in Response to Immunotherapy with the NanoString GeoMx Platform
      Characterizing the role of B cells in Response to Immunotherapy...
      Digital spatial profiling (DSP) on the NanoString GeoMx platform enables high plex molecular profiling of clinical tissue samples. This new profiling strategy enables deep insights into the...
      Speaker: Sarah Warren, PhD
      Presented at: Drug Discovery & Development Virtual Event Series 2020
      Sponsored By: NanoString Technologies
      1
      FEB 25, 2020 | 9:00 AM
      CAR-T Generation for Identity, Purity and Potency Assay Testing
      CAR-T Generation for Identity, Purity and Potency Assay Testing
      Learn about how to generate a small scale CAR-T workflow using ThermoFisher products See detailed characterization tools that can be utilized and applied in a CAR-T workflow...
      Speaker: Tia Hexom, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      10 1
      FEB 05, 2020 | 8:00 AM
      C.E. CREDITS
      Emerging Issues in the Coagulation Laboratory
      Emerging Issues in the Coagulation Laboratory
      Learn how new hemophilia and anticoagulant therapies impact coagulation assays and clinical decisions in our educational session....
      Speaker: Donna Castellone, MS, MASCP, MT(ASCP) SH
      Presented at: Stago EdVantage Virtual University
      Sponsored By: Diagnostica Stago, Inc.
      10 1
      JAN 01, 2020 | 6:00 AM
      Organoids: A Revolutionary New Tool for Drug Screening
      Organoids: A Revolutionary New Tool for Drug Screening
      “The poor translatability of early-stage preclinical models is a major setback in oncology drug development. Immortalized cell lines, that are extensively used in drug screens, undergo...
      Speaker: Rekha Pal, PhD
      Presented at: Drug Discovery & Development Virtual Event Series 2020
      1
      DEC 05, 2019 | 9:00 AM
      Morphology Driven High-Plex Spatial Analysis of Tissue Microenvironments
      Morphology Driven High-Plex Spatial Analysis of Tissue Microenvironments
      DATE: December 5, 2019TIME: 9:00am PST, 12:00pm EST Characterization of the spatial distribution and abundance of proteins and mRNAs with morphological context within tissues e...
      Speaker: Mathias Holpert, PhD , Traci DeGeer, BS, HT (ASCP) HTL, QIHC
      Sponsored By: Leica Biosystems, NanoString Technologies
      8
      DEC 04, 2019 | 7:00 AM
      Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
      Improve your reproducibility - Liquid Handling is a crucial factor...
      DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
      Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
      Sponsored By: Eppendorf
      12
    MAY 19, 2020 | 10:00 AM
    Applications in Biomarker Discovery and CAR T Therapy Using High-plex RNA & Protein Spatial Profiling on FFPE Tissue
    Applications in Biomarker Discovery and CAR T Therapy Using High-plex...
    The GeoMx Digital Spatial Profiling technology enables high-plex RNA and protein profiling of any region within any tissue type. This workshop will highlight the latest developments toward e...
    Speaker: PLENARY: Carl June, MD
    Presented at: Advancing Science - A Virtual Oncology Conference
    1
    MAY 14, 2020 | 7:05 PM
    Haemato-Oncology Applications of BCR Immune Repertoire Sequencing
    Haemato-Oncology Applications of BCR Immune Repertoire Sequencing
    B-cell lymphomas and leukemias originate from the malignant transformation and proliferation of one or a small number of B cells. Since each B-cell expresses distinct B-cell receptors (BCRs)...
    Speaker: Timothy Looney, PhD
    Presented at: OncomineWorld 2022: A Virtual NGS Education Meeting
    8
    MAY 14, 2020 | 7:05 PM
    Haemato-Oncology Applications of BCR Immune Repertoire Sequencing
    Haemato-Oncology Applications of BCR Immune Repertoire Sequencing
    B-cell lymphomas and leukemias originate from the malignant transformation and proliferation of one or a small number of B cells. Since each B-cell expresses distinct B-cell receptors (BCRs)...
    Speaker: Timothy Looney, PhD
    Presented at: OncomineWorld Autumn: A virtual NGS education meeting
    1
    MAY 14, 2020 | 3:00 PM
    Analysis of Solid Tumor Genomic Landscape in Liquid Biopsy
    Analysis of Solid Tumor Genomic Landscape in Liquid Biopsy
    Increasing evidence suggests that targeted sequencing of cell free DNA (cfDNA) can provide a comprehensive molecular portrait of solid tumors. However, the concordance between tissue and cfD...
    Speaker: Nicola Normanno, MD
    Presented at: OncomineWorld 2022: A Virtual NGS Education Meeting
    8
    MAY 14, 2020 | 3:00 PM
    Analysis of Solid Tumor Genomic Landscape in Liquid Biopsy
    Analysis of Solid Tumor Genomic Landscape in Liquid Biopsy
    Increasing evidence suggests that targeted sequencing of cell free DNA (cfDNA) can provide a comprehensive molecular portrait of solid tumors. However, the concordance between tissue and cfD...
    Speaker: Nicola Normanno, MD
    Presented at: OncomineWorld Autumn: A virtual NGS education meeting
    1
     
     
  • MAY 12, 2020 | 9:00 AM
    Analytical Quality Control for the Pharmaceutical Industry
    Analytical Quality Control for the Pharmaceutical Industry
    NEW DATE: Date: May 12, 2020 Time: 9:00am PDT, 12:00pm EDT...
    Speaker: Joy McElroy
    Sponsored By: Sartorius
    29 9
    MAY 08, 2020 | 8:00 AM
    Development of a novel molecular MSI assay with an expanded panel and automated MSI status calling
    Development of a novel molecular MSI assay with an expanded panel...
    DATE: May 8, 2020 TIME: 8:00am PT, 11:00am ET Microsatellite instability (MSI) is the condition of genetic hypermutability that results from impaired DNA mismatch repair (MMR). Studies indic...
    Speaker: Charles Baudo, Ph.D.
    Sponsored By: Thermo Fisher Scientific - Applied Biosystems
    8 2
    MAY 07, 2020 | 8:00 AM
    When Cell Growth is Critical: Evaluating COâ‚‚ Incubator Parameters for Cell Therapy
    When Cell Growth is Critical: Evaluating COâ‚‚ Incubator Parameters...
    DATE: May 7, 2020 TIME: 8:00AM PDT, 11:00AM EDT With the recent explosion of cell therapy, we know more about conditions affecting cell growth than ever before. Your CO2 incubator should not...
    Speaker: Mary Kay Bates, M.S.
    Sponsored By: Thermo Fisher Scientific
    15 2
    APR 29, 2020 | 6:00 AM
    High-Throughput assessment of ChIP-grade capability with Luminex xMAP technology
    High-Throughput assessment of ChIP-grade capability with Luminex...
    Histone post-translational modifications (PTMs) play pivotal roles in chromatin dynamics and function, with alterations in the healthy profile associated with diverse human pathologies. The...
    Speaker: Michael-Christopher Keogh, PhD
    Presented at: xMAP® Connect Virtual Conference (On-Demand)
    1
    APR 29, 2020 | 6:00 AM
    A Basic FGF Mimetic Biobetter for Mitigation of Acute Gastrointestinal Radiation Syndrome
    A Basic FGF Mimetic Biobetter for Mitigation of Acute Gastrointestinal...
    In the event of a radiological or nuclear disaster, there will be a need for therapies that can mitigate the acute hematopoietic and gastrointestinal (GI) radiation syndromes expected to dev...
    Speaker: Steven Swarts, PhD
    Presented at: xMAP® Connect Virtual Conference (On-Demand)
    1
     
     
  • APR 24, 2020 | 8:00 AM
    The International Council for Standardisation in Hematology (ICSH) recommendations for Hemostasis Critical Values, Tests, and Reporting - a stepwise description for the committee's decisions
    The International Council for Standardisation in Hematology (ICSH)...
    The International Council for Standardization in Haematology (ICSH) is a nonprofit organization dedicated to providing open-access guidance documents to enhance the quality of laboratory tes...
    Speaker: Bob Gosselin
    Sponsored By: Diagnostica Stago, Inc.
    10
    APR 22, 2020 | 8:00 AM
    C.E. CREDITS
    The NEW Coag Value Playbook...Embrace the Power of Local Testing
    The NEW Coag Value Playbook...Embrace the Power of Local Testing
    Discover strategies for labs to optimize coagulation testing, improve patient outcomes, and strengthen their role in clinical care teams....
    Speaker: Eleanor Herriman, MD, MBA , Oksana Volod, MD
    Presented at: Stago EdVantage Virtual University
    Sponsored By: Diagnostica Stago, Inc.
    7
    APR 20, 2020 | 8:00 AM
    C.E. CREDITS
    Emergency patient management using coagulation assays
    Emergency patient management using coagulation assays
    Explore emergency hemostasis testing approaches, from rapid lab assays to viscoelastic methods, for managing bleeding patients....
    Speaker: Wayne Chandler, MD
    Presented at: Stago EdVantage Virtual University
    Sponsored By: Diagnostica Stago, Inc.
    17 5
    APR 16, 2020 | 9:00 AM
    Visualize more biomarkers and extract more insights: Moving from single marker IHC to multiplex IF
    Visualize more biomarkers and extract more insights: Moving from...
    DATE: April 16, 2020 TIME: 9:00am PT, 12:00pm ET There is an increasing need to evaluate and understand unique phenotypes and mechanisms of action within the tumor microenvironment. Existing...
    Speaker: Scott Lawrence (M.S., HT(ASCP)) [C] , Katir Patel, PhD
    Sponsored By: Ultivue
    5
    APR 14, 2020 | 9:00 AM
    Gibco Cell Culture Heroes: Work From Home Edition
    Gibco Cell Culture Heroes: Work From Home Edition
    DATE: April 14, 2020 TIME: 9:00am PDT, 12:00pm EDT Besides being Gibco Cell Culture Heroes, what do Vivek, Sandra, Daisy, Ameet and Kristine all have in common? They are all stuck at home fo...
    Speaker: Kristine Wadosky, PhD , Ameet Chimote, PhD , Elizabeth Delery, PhD , Daisy Shu, B.Optom, PhD , Vivek Kamat, PhD , Sandra Hammer, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    12 1
     
     
  • APR 02, 2020 | 2:30 PM
    COVID-19 Total Solutions for Detection and Surveillance
    COVID-19 Total Solutions for Detection and Surveillance
    BGI group has been at the forefront in the fight against covid-19. Starting from building a lab in Wuhan, sequencing first 2019-nCoV virus genome and offering RT-PCR and sequencing based det...
    Speaker: Subodh Nimkar , Pan Zhang, PhD
    Presented at: Coronavirus Series
    Sponsored By: BGI Americas, MGI Americas
    2
    APR 02, 2020 | 6:00 AM
    A Multi-Omic Approach to Detection and Characterization of Viral Pathogens and Their Impact on the Immune System
    A Multi-Omic Approach to Detection and Characterization of Viral...
    A growing body of researchers around the world is working to mount an aggressive and sustained response to the COVID-19 pandemic. By building on past successes and harnessing insights from c...
    Speaker: Bill Hunt, MS , Andrew Quong, PhD
    Presented at: Coronavirus Series
    Sponsored By: Standard BioTools
    2 1
    MAR 31, 2020 | 9:00 AM
    Impact of liquid biopsy in advanced HER2+ breast cancer tumors: a follow-up study
    Impact of liquid biopsy in advanced HER2+ breast cancer tumors:...
    DATE: March 31, 2020 TIME: 9:00am PT, 12:00pm ET HER2-positive breast cancer is a type of breast cancer caused by over-expression of a protein called human epidermal growth factor receptor 2...
    Speaker: Matteo Allegretti, PhD
    Sponsored By: Thermo Fisher Scientific - Ion Torrent, Thermo Fisher Scientific - Applied Biosystems
    7
    FEB 26, 2020 | 12:00 PM
    Characterizing the role of B cells in Response to Immunotherapy with the NanoString GeoMx Platform
    Characterizing the role of B cells in Response to Immunotherapy...
    Digital spatial profiling (DSP) on the NanoString GeoMx platform enables high plex molecular profiling of clinical tissue samples. This new profiling strategy enables deep insights into the...
    Speaker: Sarah Warren, PhD
    Presented at: Drug Discovery & Development Virtual Event Series 2020
    Sponsored By: NanoString Technologies
    1
    FEB 25, 2020 | 9:00 AM
    CAR-T Generation for Identity, Purity and Potency Assay Testing
    CAR-T Generation for Identity, Purity and Potency Assay Testing
    Learn about how to generate a small scale CAR-T workflow using ThermoFisher products See detailed characterization tools that can be utilized and applied in a CAR-T workflow...
    Speaker: Tia Hexom, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    10 1
     
     
  • FEB 05, 2020 | 8:00 AM
    C.E. CREDITS
    Emerging Issues in the Coagulation Laboratory
    Emerging Issues in the Coagulation Laboratory
    Learn how new hemophilia and anticoagulant therapies impact coagulation assays and clinical decisions in our educational session....
    Speaker: Donna Castellone, MS, MASCP, MT(ASCP) SH
    Presented at: Stago EdVantage Virtual University
    Sponsored By: Diagnostica Stago, Inc.
    10 1
    JAN 01, 2020 | 6:00 AM
    Organoids: A Revolutionary New Tool for Drug Screening
    Organoids: A Revolutionary New Tool for Drug Screening
    “The poor translatability of early-stage preclinical models is a major setback in oncology drug development. Immortalized cell lines, that are extensively used in drug screens, undergo...
    Speaker: Rekha Pal, PhD
    Presented at: Drug Discovery & Development Virtual Event Series 2020
    1
    DEC 05, 2019 | 9:00 AM
    Morphology Driven High-Plex Spatial Analysis of Tissue Microenvironments
    Morphology Driven High-Plex Spatial Analysis of Tissue Microenvironments
    DATE: December 5, 2019TIME: 9:00am PST, 12:00pm EST Characterization of the spatial distribution and abundance of proteins and mRNAs with morphological context within tissues e...
    Speaker: Mathias Holpert, PhD , Traci DeGeer, BS, HT (ASCP) HTL, QIHC
    Sponsored By: Leica Biosystems, NanoString Technologies
    8
    DEC 04, 2019 | 7:00 AM
    Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
    Improve your reproducibility - Liquid Handling is a crucial factor...
    DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
    Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
    Sponsored By: Eppendorf
    12
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