JAN 31, 2019 10:30 AM PST

Flow-Through Polishing and The Use of Multi-Mode Ligand Libraries to Improve Process Efficiencies

Presented at: BioProcessing 2019
Sponsored by: Astrea Bioseparations
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Product Support & Development Manager at Prometic Bioseparations Ltd
    Biography
      As the Product Support & Development (PSD) Manager for Prometic Bioseparations Ltd, Buzz leads a small dedicated team providing full technical support and information for PBL's products.
      Buzz joined PBL in 2004 as a Research Scientist in the downstream purification team. In 2011 he became the Applications & Downstream Processing Specialist, and in 2016 was appointed the new role of PSD Manager.

      Buzz obtained his Biochemistry degree from University of Kent, then spent over 14 years in both the Research (University of Cambridge) and Biotech/Biopharmaceutical sectors (Adprotech, Metris and Celltech (now UCB)) before joining Prometic. He has significant product profiling and processing experience having worked on small-scale purification applications to large-scale manufacturing processes.

    Abstract:

    Multi-mode chromatography has come to prominence in recent years due to the general utility of multi-mode ligands for product capture and polishing. In particular, their application in flow-through polishing has significantly improved process economy. However, significant optimisation of binding and elution conditions is often required to achieve the desired level of purity.

    To overcome the deficiencies of individual multi-mode ligands, we have developed a diverse library of Multi-Mode Mimetic Ligands™  incorporating both ionic and hydrophobic groups, which offer significant flexibility in use. The Multi-Mode Mimetic Ligand™ Library is organised into a 12 x 8 array micro-column block and comprises a diverse array of ligands with a broad spread of charge, hydrophobic and hydrophilic functionalities. The adsorbents are produced using an agarose support matrix and incorporate triazine attachment chemistry for ease of synthesis and superior stability, Screening can be performed in high-throughput mode using robotic liquid handling systems or by use of conventional hand-held multi-channel pipettes. This approach allows for rapid screening, and identification of potential ligands for a specific target. Furthermore, adjustment of the starting protein fraction to suit individual ligands is not required as the diversity of the library enables the identification of binding ligands which are best-suited to the existing conditions and impurities.

    This approach has been evaluated in a variety of applications, including development of ligands for the removal of specific contaminants in a flow through mode.

    Learning Objectives: 

    1. Understand how you can use a diverse library of synthetic ligands to screen for adsorbents for your molecules that are difficult to purify using standard adsorbents. The screening library is fast becoming a must-have technology in the tool-box of a process development scientist.
    2. A case study of using the Multi-Mode Mimetic Ligand™ Library to deliver a flow-through polishing step to remove FXI from human blood plasma.


    Show Resources
    You May Also Like
    SEP 05, 2019 4:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 4:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    AUG 27, 2019 9:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 9:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    FEB 26, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    FEB 19, 2020 11:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    FEB 19, 2020 11:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: February 19, 2020TIME: 11:00am PST, 2:00pm EST...
    Loading Comments...
    Show Resources
    Attendees