Informatics and internet are widely accepted tools in education and research. In the past decades new teaching and learning strategies have been developed and implemented. E-learning was defined as the production, distribution and use of electronic content for educational purposes. Many institutions and organizations in Europe and USA offer Laboratory Animal Science (LAS) training including computer assisted learning (CAL) or online tools in their teaching curriculum and as educational resources, for example the AALAS Learning Library and NC3Rs web. While these programs are very useful to introduce necessary theoretical background and skills, most of them are in English and based only in audiovisual materials. The aim of this presentation is to show the contribution of this methodology in the harmonization of the humane treatment of experimental animals mainly in Latin America countries, where there is still a strong need of animals in research and education. By other hand, one of the most important requisite worldwide is that personnel who work with laboratory animals are well trained with special emphasis in the 3Rs principles. Moreover, there is a lack of harmonized training guidelines and poor offer of training. We are going to show why and how a well-planned e-learning program is a valuable tool to mediate knowledge in LAS training between participants from mainly Latin America and developing countries. Our experience shows that replacement and reduce of animals in a first approach is possible with this education strategy.