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    CAR-T cell therapy

    Chimeric antigen receptor (CAR) T cell therapy is a form of T cell engineering where T cells are obtained from a patient and engineered in a laboratory to better kill the patient's cancer cells. Specifically, CAR T cells are modified to express a receptor on their surface that recognizes the specific antigen present on the patient's cancer cells.

    Webinars

    • APR 06, 2021 | 8:00 AM
      Make your research count - Generate reproducible results with recombinant antibodies
      Make your research count - Generate reproducible results with...
      Date: April 06, 2021 Time: 8:00am (PST), 11:00am (EST) The discovery of the reproducibility crisis has called into question a worrying number of scientific results over the last few years. In...
      Speaker: Urmi Roy, PhD
      Sponsored By: Miltenyi Biotec
      5
      MAR 31, 2021 | 8:00 AM
      CytoFLEX SRT Cell Sorter: What is it really like for a user?
      CytoFLEX SRT Cell Sorter: What is it really like for a user?
      Date: March 31, 2021 Time: 08:00am PST CytoFLEX SRT has enabled researcher lead self-service sorting. The need for a cell sorting expert to setup and maintain the sorter status is now necess...
      Speaker: Peter O'Toole, PhD , Dr. Karen Hogg
      Sponsored By: Beckman Coulter Life Sciences
      7
      MAR 30, 2021 | 8:00 AM
      Utilizing the Gibco™ CTS™ Rotea™ System, a versatile closed manufacturing system, for cell and gene therapy development
      Utilizing the Gibco™ CTS™ Rotea™ System, a versatile closed manufacturing...
      DATE: March 30, 2021 TIME: 08:00am PST In this talk, we will discuss the value of moving towards modular and automated, closed-system technologies designed to enable scalable and cost-effect...
      Speaker: Mary Ann Santos , Emily Benz , Eric Dubofsky
      Sponsored By: Thermo Fisher Scientific/Gibco
      6
      FEB 10, 2021 | 8:00 AM
      Unveil the immune response to cancer and infectious disease with powerful immunoassays
      Unveil the immune response to cancer and infectious disease with...
      Date: February 10, 2021 Time: 8:00am (PST), 11:00am (EST) Uncovering the interactions between the immune system and cancer cells or pathogens is key for understanding and controlling both ca...
      Speaker: Sigrun Badrnya, PhD
      Sponsored By: Thermo Fisher Scientific
      14 1
      FEB 03, 2021 | 8:00 AM
      An introduction to immuno-oncology at Miltenyi Biotec
      An introduction to immuno-oncology at Miltenyi Biotec
      Date: February 3, 2021 Time: 8:00am (PST), 11:00am (EST) This introduction webinar will provide you with an overview of the general immuno-oncology (IO) research field...
      Speaker: Dr. Marcello Stein
      Sponsored By: Miltenyi Biotec
      19 2
      NOV 17, 2020 | 8:00 AM
      Key Features of Immune Cells During SARS-CoV2 Infection
      Key Features of Immune Cells During SARS-CoV2 Infection
      Date: November 17, 2020 Time: 8:00am COVID-19 is a complex pathological condition caused by infection with SARS-CoV. Different features have been observed in patients with a severe dis...
      Speaker: Sara De Biasi
      Sponsored By: Thermo Fisher Scientific
      13
      NOV 05, 2020 | 9:00 AM
      Cell-based Assays to Functionally Characterize Immunotherapy and the Immune Response to Viral Infection
      Cell-based Assays to Functionally Characterize Immunotherapy and...
      DATE: November 5, 2020 TIME: 9:00am (PDT), 12:00pm (EDT) With accelerating development and approval of cell therapies, biologic drugs, and antiviral treatments comes a need for more robust a...
      Speaker: Chris Langsdorf
      Sponsored By: Thermo Fisher Scientific
      8 1
      NOV 04, 2020 | 11:00 AM
      Developing flow-based analytical methods for gene-edited CAR-T programs: How to ensure an efficient transition from early research to clinical development
      Developing flow-based analytical methods for gene-edited CAR-T...
      DATE: November 4th, 2020 TIME: 11:00am PST As cell therapies become more complex, the need for robust analytical tools to characterize such products as they enter the clinic has increased in...
      Speaker: Aaron Edwards
      Sponsored By: Miltenyi Biotec
      6
      SEP 23, 2020 | 12:00 PM
      Mass Cytometry in Translational Research: Session II
      Mass Cytometry in Translational Research: Session II
      B cells and tertiary lymphoid structures (TLS) promote immunotherapy response 1:00–1:30 pm PDT Presented By: Rafet Basar, MD...
      Speaker: Handan Xiang, PhD , Pei-Yu Chen, PhD , Hiranmayi Ravichandran, MS , Rafet Basar, MD , Amir Horowitz, PhD
      Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
      2
      SEP 23, 2020 | 10:30 AM
      Practical Mass Cytometry
      Practical Mass Cytometry
      On measuring photons and ions: Impact on panel design, signal detection and data quality 10:30–11:00 am PDT Presented By: Tim Bushnell, PhD, MBA Monitoring immunotherapy with a mass cy...
      Speaker: Tim Bushnell, PhD, MBA , Gerd Haga Bringeland, MD, PhD , Greg Hopkins, BS
      Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
      2
      SEP 23, 2020 | 6:30 AM
      Mass Cytometry in Translational Research: Session I
      Mass Cytometry in Translational Research: Session I
      Single-cell mass cytometry identifies mechanisms of resistance to immunotherapy in AML 6:30–7:00 am PDT Presented By: Shelley Herbrich, PhD Understanding CD19 negative relapse followin...
      Speaker: Shelley Herbrich, PhD , Kara L. Davis, DO , Bernd Bodenmiller, PhD
      Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
      2
      AUG 04, 2020 | 8:00 AM
      Flexible, clinically adaptable, non-viral approaches to CAR TCR methodologies
      Flexible, clinically adaptable, non-viral approaches to CAR TCR...
      DATE: June 23, 2020 TIME: 6:00 am PDT, 9:00 am EDT In this webinar for scientists and researchers, Rama Shivakumar, a senior scientist at MaxCyte Inc., will highlight powerful case studies t...
      Speaker: Rama S Shivakumar
      Sponsored By: MaxCyte®
      7
      JUL 29, 2020 | 2:00 PM
      Route of 41BB/41BBL costimulation determines effector function of B7-H3-CAR.CD28ζ T cells
      Route of 41BB/41BBL costimulation determines effector function...
      B7-H3 is actively being explored as an immunotherapy target for pediatric patients with solid tumors using monoclonal antibodies or T cells expressing chimeric antigen receptors (CARs). B7-H...
      Speaker: Christopher DeRenzo, MD
      Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
      1
      JUL 29, 2020 | 9:00 AM
      Impedance-based assay to evaluate potency of immunotherapy products
      Impedance-based assay to evaluate potency of immunotherapy products
      Advances in genetic engineering have improved specificity, safety and potency of T-cell receptor (TCR) and Chimeric Antigen Receptor (CAR-T) based therapies for solid and non-solid tumours....
      Speaker: Juan Miguel Sanchez Nieto, PhD
      Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
      1
      JUL 22, 2020 | 10:00 AM
      Molecular Diagnostic Solutions in a Pandemic: Planning for the 2020/2021 Flu Season with the Implementation of the ePlex® Respiratory Pathogen Panel 2
      Molecular Diagnostic Solutions in a Pandemic: Planning for the...
      DATE: July 23, 2020 TIME: 10:00 am PDT The SARS-CoV-2 pandemic has taken a toll on many sectors of the medical community. As the pandemic took a grip on the laboratory, the need for diagnost...
      Speaker: Heba Mostafa, MD, PhD, D(ABMM)
      Sponsored By: GenMark Dx
      27 13
      JUN 30, 2020 | 9:00 AM
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
      DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
      Speaker: Jane Weitzel
      Sponsored By: Sartorius
      17 1
      JUN 17, 2020 | 1:30 PM
      Will we have a vaccine for COVID-19? 5 reasons for optimism!
      Will we have a vaccine for COVID-19? 5 reasons for optimism!
      The COVID-19 pandemic has resulted in over 7 million cases and more than 428000 cases to date. While public health interventions and social distancing measures have been effective in slowing...
      Speaker: Rob Kozak, PhD FCCM
      Presented at: Coronavirus Series
      1
      JUN 17, 2020 | 7:30 AM
      Development of Luminex Based Serological Test for COVID-19 Diagnosis and Monitoring
      Development of Luminex Based Serological Test for COVID-19 Diagnosis...
      Coronavirus disease 2019 (Covid-19) is a global pandemic that has impacted the lives of the entire world’s population. Accurate and effective testing mechanisms to identify those with...
      Speaker: Imad Tarhoni, MD, PhD
      Presented at: Coronavirus Series
      Sponsored By: Luminex - A DiaSorin Company
      2
      JUN 03, 2020 | 12:00 PM
      Single cell multiomic analysis of T-cell exhaustion
      Single cell multiomic analysis of T-cell exhaustion
      A key step in the clinical production of CAR T cells is the expansion of engineered T cells. To generate enough cells for viable adoptive cell therapy, cells must be robustly stimulated, whi...
      Speaker: Pratip K. Chattopadhyay
      Presented at: Immuno-Oncology Virtual Event Series 2020
      Sponsored By: BD
      2
      JUN 03, 2020 | 12:00 PM
      How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
      How multiplexed imaging reveals key insights into the tumor microenvironment...
      Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understanding of cells and their surroundings by providin...
      Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
      Presented at: Immuno-Oncology Virtual Event Series 2020
      Sponsored By: Cytiva
      0
      JUN 03, 2020 | 7:00 AM
      High throughput biomarker profiling for immuno-oncology research using MILLIPLEX® assays
      High throughput biomarker profiling for immuno-oncology research...
      Dr. Koenitzer will present an overview of her current research in immune suppression. After a brief introduction to the MILLIPLEX® multiplex immunoassay portfolio, Dr. Koenitzer will dis...
      Speaker: Jenny Koenitzer, PhD , Yan Nikhamin, MS, MBA
      Presented at: Immuno-Oncology Virtual Event Series 2020
      Sponsored By: MilliporeSigma
      1
      JUN 03, 2020 | 12:00 AM
      Microenvironment on Demand: A breakthrough technology for personalized cell therapy discoveries
      Microenvironment on Demand: A breakthrough technology for personalized...
      NCI estimates that cancer will be the leading cause of death in 2030, worldwide. Checkpoint inhibitors and adoptive cell therapies (ACTs) cost up to ~$2 million/patient and have shown durabl...
      Speaker: Maithreyan Srinivasan
      Presented at: Immuno-Oncology Virtual Event Series 2020
      1
      JUN 02, 2020 | 7:00 AM
      Harnessing the Power of Protein Expression Systems in Vaccine Development
      Harnessing the Power of Protein Expression Systems in Vaccine...
      DATE: June 2, 2020 TIME: 7:00am PT, 10:00am ET In just a few months COVID-19 has risen from a regional crisis to a global threat, and drug makers are now scrambling to develop vaccines aimed...
      Speaker: Roland Leathers, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      15 1
      MAY 27, 2020 | 8:00 AM
      How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
      How multiplexed imaging reveals key insights into the tumor microenvironment...
      DATE: May 27, 2020 TIME: 8:00am PT, 11:00am ET Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understand...
      Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
      Sponsored By: Cytiva
      18 2
    APR 06, 2021 | 8:00 AM
    Make your research count - Generate reproducible results with recombinant antibodies
    Make your research count - Generate reproducible results with...
    Date: April 06, 2021 Time: 8:00am (PST), 11:00am (EST) The discovery of the reproducibility crisis has called into question a worrying number of scientific results over the last few years. In...
    Speaker: Urmi Roy, PhD
    Sponsored By: Miltenyi Biotec
    5
    MAR 31, 2021 | 8:00 AM
    CytoFLEX SRT Cell Sorter: What is it really like for a user?
    CytoFLEX SRT Cell Sorter: What is it really like for a user?
    Date: March 31, 2021 Time: 08:00am PST CytoFLEX SRT has enabled researcher lead self-service sorting. The need for a cell sorting expert to setup and maintain the sorter status is now necess...
    Speaker: Peter O'Toole, PhD , Dr. Karen Hogg
    Sponsored By: Beckman Coulter Life Sciences
    7
    MAR 30, 2021 | 8:00 AM
    Utilizing the Gibco™ CTS™ Rotea™ System, a versatile closed manufacturing system, for cell and gene therapy development
    Utilizing the Gibco™ CTS™ Rotea™ System, a versatile closed manufacturing...
    DATE: March 30, 2021 TIME: 08:00am PST In this talk, we will discuss the value of moving towards modular and automated, closed-system technologies designed to enable scalable and cost-effect...
    Speaker: Mary Ann Santos , Emily Benz , Eric Dubofsky
    Sponsored By: Thermo Fisher Scientific/Gibco
    6
    FEB 10, 2021 | 8:00 AM
    Unveil the immune response to cancer and infectious disease with powerful immunoassays
    Unveil the immune response to cancer and infectious disease with...
    Date: February 10, 2021 Time: 8:00am (PST), 11:00am (EST) Uncovering the interactions between the immune system and cancer cells or pathogens is key for understanding and controlling both ca...
    Speaker: Sigrun Badrnya, PhD
    Sponsored By: Thermo Fisher Scientific
    14 1
    FEB 03, 2021 | 8:00 AM
    An introduction to immuno-oncology at Miltenyi Biotec
    An introduction to immuno-oncology at Miltenyi Biotec
    Date: February 3, 2021 Time: 8:00am (PST), 11:00am (EST) This introduction webinar will provide you with an overview of the general immuno-oncology (IO) research field...
    Speaker: Dr. Marcello Stein
    Sponsored By: Miltenyi Biotec
    19 2
     
     
  • NOV 17, 2020 | 8:00 AM
    Key Features of Immune Cells During SARS-CoV2 Infection
    Key Features of Immune Cells During SARS-CoV2 Infection
    Date: November 17, 2020 Time: 8:00am COVID-19 is a complex pathological condition caused by infection with SARS-CoV. Different features have been observed in patients with a severe dis...
    Speaker: Sara De Biasi
    Sponsored By: Thermo Fisher Scientific
    13
    NOV 05, 2020 | 9:00 AM
    Cell-based Assays to Functionally Characterize Immunotherapy and the Immune Response to Viral Infection
    Cell-based Assays to Functionally Characterize Immunotherapy and...
    DATE: November 5, 2020 TIME: 9:00am (PDT), 12:00pm (EDT) With accelerating development and approval of cell therapies, biologic drugs, and antiviral treatments comes a need for more robust a...
    Speaker: Chris Langsdorf
    Sponsored By: Thermo Fisher Scientific
    8 1
    NOV 04, 2020 | 11:00 AM
    Developing flow-based analytical methods for gene-edited CAR-T programs: How to ensure an efficient transition from early research to clinical development
    Developing flow-based analytical methods for gene-edited CAR-T...
    DATE: November 4th, 2020 TIME: 11:00am PST As cell therapies become more complex, the need for robust analytical tools to characterize such products as they enter the clinic has increased in...
    Speaker: Aaron Edwards
    Sponsored By: Miltenyi Biotec
    6
    SEP 23, 2020 | 12:00 PM
    Mass Cytometry in Translational Research: Session II
    Mass Cytometry in Translational Research: Session II
    B cells and tertiary lymphoid structures (TLS) promote immunotherapy response 1:00–1:30 pm PDT Presented By: Rafet Basar, MD...
    Speaker: Handan Xiang, PhD , Pei-Yu Chen, PhD , Hiranmayi Ravichandran, MS , Rafet Basar, MD , Amir Horowitz, PhD
    Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
    2
    SEP 23, 2020 | 10:30 AM
    Practical Mass Cytometry
    Practical Mass Cytometry
    On measuring photons and ions: Impact on panel design, signal detection and data quality 10:30–11:00 am PDT Presented By: Tim Bushnell, PhD, MBA Monitoring immunotherapy with a mass cy...
    Speaker: Tim Bushnell, PhD, MBA , Gerd Haga Bringeland, MD, PhD , Greg Hopkins, BS
    Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
    2
     
     
  • SEP 23, 2020 | 6:30 AM
    Mass Cytometry in Translational Research: Session I
    Mass Cytometry in Translational Research: Session I
    Single-cell mass cytometry identifies mechanisms of resistance to immunotherapy in AML 6:30–7:00 am PDT Presented By: Shelley Herbrich, PhD Understanding CD19 negative relapse followin...
    Speaker: Shelley Herbrich, PhD , Kara L. Davis, DO , Bernd Bodenmiller, PhD
    Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
    2
    AUG 04, 2020 | 8:00 AM
    Flexible, clinically adaptable, non-viral approaches to CAR TCR methodologies
    Flexible, clinically adaptable, non-viral approaches to CAR TCR...
    DATE: June 23, 2020 TIME: 6:00 am PDT, 9:00 am EDT In this webinar for scientists and researchers, Rama Shivakumar, a senior scientist at MaxCyte Inc., will highlight powerful case studies t...
    Speaker: Rama S Shivakumar
    Sponsored By: MaxCyte®
    7
    JUL 29, 2020 | 2:00 PM
    Route of 41BB/41BBL costimulation determines effector function of B7-H3-CAR.CD28ζ T cells
    Route of 41BB/41BBL costimulation determines effector function...
    B7-H3 is actively being explored as an immunotherapy target for pediatric patients with solid tumors using monoclonal antibodies or T cells expressing chimeric antigen receptors (CARs). B7-H...
    Speaker: Christopher DeRenzo, MD
    Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
    1
    JUL 29, 2020 | 9:00 AM
    Impedance-based assay to evaluate potency of immunotherapy products
    Impedance-based assay to evaluate potency of immunotherapy products
    Advances in genetic engineering have improved specificity, safety and potency of T-cell receptor (TCR) and Chimeric Antigen Receptor (CAR-T) based therapies for solid and non-solid tumours....
    Speaker: Juan Miguel Sanchez Nieto, PhD
    Presented at: Immuno-Oncology & Cancer Biology Virtual Conference
    1
    JUL 22, 2020 | 10:00 AM
    Molecular Diagnostic Solutions in a Pandemic: Planning for the 2020/2021 Flu Season with the Implementation of the ePlex® Respiratory Pathogen Panel 2
    Molecular Diagnostic Solutions in a Pandemic: Planning for the...
    DATE: July 23, 2020 TIME: 10:00 am PDT The SARS-CoV-2 pandemic has taken a toll on many sectors of the medical community. As the pandemic took a grip on the laboratory, the need for diagnost...
    Speaker: Heba Mostafa, MD, PhD, D(ABMM)
    Sponsored By: GenMark Dx
    27 13
     
     
  • JUN 30, 2020 | 9:00 AM
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
    DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
    Speaker: Jane Weitzel
    Sponsored By: Sartorius
    17 1
    JUN 17, 2020 | 1:30 PM
    Will we have a vaccine for COVID-19? 5 reasons for optimism!
    Will we have a vaccine for COVID-19? 5 reasons for optimism!
    The COVID-19 pandemic has resulted in over 7 million cases and more than 428000 cases to date. While public health interventions and social distancing measures have been effective in slowing...
    Speaker: Rob Kozak, PhD FCCM
    Presented at: Coronavirus Series
    1
    JUN 17, 2020 | 7:30 AM
    Development of Luminex Based Serological Test for COVID-19 Diagnosis and Monitoring
    Development of Luminex Based Serological Test for COVID-19 Diagnosis...
    Coronavirus disease 2019 (Covid-19) is a global pandemic that has impacted the lives of the entire world’s population. Accurate and effective testing mechanisms to identify those with...
    Speaker: Imad Tarhoni, MD, PhD
    Presented at: Coronavirus Series
    Sponsored By: Luminex - A DiaSorin Company
    2
    JUN 03, 2020 | 12:00 PM
    Single cell multiomic analysis of T-cell exhaustion
    Single cell multiomic analysis of T-cell exhaustion
    A key step in the clinical production of CAR T cells is the expansion of engineered T cells. To generate enough cells for viable adoptive cell therapy, cells must be robustly stimulated, whi...
    Speaker: Pratip K. Chattopadhyay
    Presented at: Immuno-Oncology Virtual Event Series 2020
    Sponsored By: BD
    2
    JUN 03, 2020 | 12:00 PM
    How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
    How multiplexed imaging reveals key insights into the tumor microenvironment...
    Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understanding of cells and their surroundings by providin...
    Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
    Presented at: Immuno-Oncology Virtual Event Series 2020
    Sponsored By: Cytiva
    0
     
     
  • JUN 03, 2020 | 7:00 AM
    High throughput biomarker profiling for immuno-oncology research using MILLIPLEX® assays
    High throughput biomarker profiling for immuno-oncology research...
    Dr. Koenitzer will present an overview of her current research in immune suppression. After a brief introduction to the MILLIPLEX® multiplex immunoassay portfolio, Dr. Koenitzer will dis...
    Speaker: Jenny Koenitzer, PhD , Yan Nikhamin, MS, MBA
    Presented at: Immuno-Oncology Virtual Event Series 2020
    Sponsored By: MilliporeSigma
    1
    JUN 03, 2020 | 12:00 AM
    Microenvironment on Demand: A breakthrough technology for personalized cell therapy discoveries
    Microenvironment on Demand: A breakthrough technology for personalized...
    NCI estimates that cancer will be the leading cause of death in 2030, worldwide. Checkpoint inhibitors and adoptive cell therapies (ACTs) cost up to ~$2 million/patient and have shown durabl...
    Speaker: Maithreyan Srinivasan
    Presented at: Immuno-Oncology Virtual Event Series 2020
    1
    JUN 02, 2020 | 7:00 AM
    Harnessing the Power of Protein Expression Systems in Vaccine Development
    Harnessing the Power of Protein Expression Systems in Vaccine...
    DATE: June 2, 2020 TIME: 7:00am PT, 10:00am ET In just a few months COVID-19 has risen from a regional crisis to a global threat, and drug makers are now scrambling to develop vaccines aimed...
    Speaker: Roland Leathers, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    15 1
    MAY 27, 2020 | 8:00 AM
    How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
    How multiplexed imaging reveals key insights into the tumor microenvironment...
    DATE: May 27, 2020 TIME: 8:00am PT, 11:00am ET Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understand...
    Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
    Sponsored By: Cytiva
    18 2
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