MAR 31, 2021 8:00 AM PDT

CytoFLEX SRT Cell Sorter: What is it really like for a user?

Speakers
  • Experimental Officer - Imaging and Cytometry (KGH)
    Biography
      Karen Hogg is an Experimental Officer: Imaging and Cytometry at the University of York, Bioscience Technology Facility. Karen underpins the scientific service within the Imaging and Cytometry Laboratory and takes a lead role in the research, operation and method development of cell sorting and analysis. As such, Karen's expertise is utilized for a wide range of diverse applications both within and external to the Department of Biology. Karen is also a co-organizer and tutor on multiple flow cytometry courses. She obtained her PhD from the School of Biology, University of Leeds, U.K. 1999 and worked in a parasite immunology research group for 4 years before taking up her current role. Karen was awarded the Royal Microscopical Society medal for Flow Cytometry in 2016.
    • Director of the Bioscience Technology Facility, Head of Imaging Cytometry, Department of Biology, University of York
      Biography
        Peter is Director of the Bioscience Technology Facility at the University of York covering the broad technologies required for a multi-disciplined Department of Biology.

        He has also built up and heads the highly successful Imaging and Cytometry Labs which includes an array of confocal microscopes, flow cytometers, electron microscopes and novel instrumentation. Peter gained his PhD in the Cell Biophysics Laboratory at Essex and has been involved in many aspects of fluorescence imaging and cytometry. Research is currently focused on both technology and method development of novel probes and imaging modalities. His lab provides research support to many academics and commercial organisations and he has ongoing collaborations and consultancy contracts with many leading microscopy and cytometry companies.

        Peter serves on an array of committees for e.g. BBSRC , Vice-President of the RMS and Steering committee for ELMI. Peter is also heavily involved with microscopy and cytometry training through a variety of international courses.

      Abstract

      Date:  March 31, 2021

      Time: 8:00am PDT, 5:00pm CEST

       

      The University of York has now been using the new benchtop cell sorter CytoFLEX SRT for several months. We will talk about our experiences with this new system, whilst also running live samples with quality checks throughout! Using multiple cameras and desktop sharing, we will try and make this as close as possible to being in the labs yourselves. 
      As a user friendly system, at the start of the seminar, we will 'Go Live' and switch the system on for all to see. Whilst the system initialises, sets itself up, we will talk you through the various features of the CytoFLEX SRT that appeal to ourselves and our user base. 


      We will then return to the CytoFLEX SRT to set up a live sort on fluorescent tagged cells and to show that beyond the basic bulk sorting into tubes, that the 96 well plate sorting is equally simple. This will also enable us to show the precision of sorting cells, as we will go to the microscopes to physically count the number of cells sorted into each well. 
      We will then also go through some of the different user sample examples that we have so far proven on the system, including very sensitive stem cell sorts. 
      Seeing is believing!: So we will show you in real time the ease of setting up and sorting with the CytoFLEX SRT and describe our first insights and impressions of this benchtop cell sorter.

       

      Learning Objectives:

      • Explain how the SRT works
      • Discuss what we have used to date on the system
      • Identify and visualize the system in action

       

       

       

      Webinars will be available for unlimited on-demand viewing after live event.

       

       


      Show Resources
      You May Also Like
      MAY 11, 2021 10:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      MAY 11, 2021 10:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
      DEC 02, 2020 8:00 AM PST
      C.E. CREDITS
      DEC 02, 2020 8:00 AM PST
      DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
      NOV 18, 2020 8:00 AM PST
      C.E. CREDITS
      NOV 18, 2020 8:00 AM PST
      DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
      JUN 09, 2021 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      JUN 09, 2021 7:00 AM PDT
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
      APR 01, 2021 8:00 AM PDT
      C.E. CREDITS
      APR 01, 2021 8:00 AM PDT
      Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
      NOV 16, 2020 8:00 AM PST
      C.E. CREDITS
      NOV 16, 2020 8:00 AM PST
      Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
      Loading Comments...
      Show Resources
      Attendees
      • See more