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    Cell staining

    Cell staining is a laboratory procedure used to visualize cells with microscopy. Different stains can be used to identify specific cellular components. Some examples of cell staining include 7-AAD, DAPI, Hoechst dye, propidium iodine, and trypan blue.

    Webinars

    • OCT 06, 2021 | 9:00 AM
      Antibody titration for flow cytometric analysis of Extracellular Vesicles: Are cell based protocols up to the task?
      Antibody titration for flow cytometric analysis of Extracellular...
      Date: October 6, 2021 Time: 9:00am (PDT), 12:00pm (EDT) ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis...
      Speaker: Desmond Pink, PhD
      Sponsored By: Miltenyi Biotec
      6 1
      SEP 29, 2021 | 10:00 AM
      Study design: How and when to use multiplexed immunofluorescence imaging in your research
      Study design: How and when to use multiplexed immunofluorescence...
      Date: September 29, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Multiplexed tissue analysis is a powerful technique that allows comparisons of cell-type locations and cell-type interactions withi...
      Speaker: Melinda Angus-Hill Ph.D.
      Sponsored By: Leica Microsystems
      5
      SEP 22, 2021 | 11:00 PM
      Failing Tethers Destabilize Neutrophil Rolling and Form Elongated Neutrophil-Derived Structures (ENDS) in the Blood Circulation
      Failing Tethers Destabilize Neutrophil Rolling and Form Elongated...
      Neutrophils are the most abundant immune cells in human blood and are crucial for host defense against bacterial and fungal infections. Neutrophils are made mainly in the bone marrow from wh...
      Speaker: Alex Marki
      Presented at: Cell Biology Virtual Event Series 2021
      1
      JUN 17, 2021 | 7:00 AM
      Cell DIVE: a robust platform for multiplexed whole slide imaging and single-cell analysis
      Cell DIVE: a robust platform for multiplexed whole slide imaging...
      DATE: June 17, 2021 TIME: 07:00am PDT Colorectal cancer is a particularly challenging disease for immunotherapy given tumor heterogeneity. Multiplexed whole slide imaging allows deep context...
      Speaker: Melinda Angus-Hill Ph.D.
      Sponsored By: Leica Microsystems
      8
      JUN 16, 2021 | 8:00 AM
      Simplicity that defies complexity - the MACSima™ Platform for ultrahigh-content imaging
      Simplicity that defies complexity - the MACSima™ Platform for...
      Date: June 16, 2021 Time: 8:00am PDT The MACSima™ Imaging Platform is the only complete solution for ultrahigh-content imaging of hundreds of markers, allowing you to understand the tu...
      Speaker: Dr. Ali Kinkhabwala , Dr. Markus Habich
      Sponsored By: Miltenyi Biotec
      7
      JUN 02, 2021 | 9:00 AM
      Making sense out of multiplex
      Making sense out of multiplex
      The Tumor Microenvironment (TME) is a highly complex niche, with many different cell types characterized by a range of biomarkers. Clever staining protocols like Cycle Immunofluorescence, an...
      Speaker: Dave Mason, PhD
      Presented at: Immuno-Oncology Virtual Event Series 2021
      Sponsored By: Visiopharm®
      4
      JUN 02, 2021 | 8:00 AM
      Exploiting digital histology approaches to probe the pathophysiology of SARS-CoV-2 infection
      Exploiting digital histology approaches to probe the pathophysiology...
      Date: June 2, 2021 Time: 8:00am (PDT), 11:00am (EDT) In this introduction Kelly Hunter will address the first Learning Objective. He will cover i) a range of digital pathology and tissue mul...
      Speaker: Kelly Hunter, BSc Biochemistry, MSc Genomics Medicine , Dr. Graham Taylor, BSc, Ph.D
      Sponsored By: Leica Biosystems
      11 1
      MAY 26, 2021 | 9:00 AM
      Advanced Phenotyping of the Tumor Immune Microenvironment
      Advanced Phenotyping of the Tumor Immune Microenvironment
      Date: May 26, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Immunotherapy has transformed the treatment of metastatic and recurrent solid tumors but is challenged in that only a minority of patie...
      Speaker: Angela Vasaturo, Ph.D. , Fabian Schneider, Ph.D.
      Sponsored By: Ultivue
      6 1
      APR 29, 2021 | 10:00 AM
      Quantitative western blotting analysis: tips, considerations, and streamlining analysis using Invitrogen iBright Analysis Software
      Quantitative western blotting analysis: tips, considerations,...
      Date: April 29, 2021 Time: 10:00am PDT Western blotting is a fundamental application used in life science research to better understand target proteins and their function in complex systems....
      Speaker: Janaki Narahari, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      5
      APR 28, 2021 | 9:00 AM
      Combining protein proximity detection with chromogenic multiplex: Discovering the PD1/PDL1 interactions
      Combining protein proximity detection with chromogenic multiplex:...
      Proximity Ligation Assay (PLA), is a powerful technique for demonstrating the presence of 2 unique targets in direct proximity of each other in tissue sections. It allows visualization of in...
      Speaker: Thierry P.P. van den Bosch, PhD
      Presented at: DISCOVERY Global User Symposium
      3
      APR 21, 2021 | 5:00 PM
      Immunology Researchers share their Spatial Discoveries in SARS-CoV-2 host response and in multiple sclerosis
      Immunology Researchers share their Spatial Discoveries in SARS-CoV-2...
      Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
      Speaker: Doug Brown, PhD , Susanne Krasemann, MD, PhD , Michelle Naughton, PhD
      Sponsored By: NanoString Technologies, Illumina
      5
      MAR 16, 2021 | 8:00 AM
      The latest developments for enriching and analyzing antigen- and virus-specific T cells
      The latest developments for enriching and analyzing antigen- and...
      DATE: March 09, 2021 TIME: 08:00am PST The COVID-19 pandemic has placed urgency on precise analysis of rare antigen- and virus-specific T cells. At Miltenyi Biotec, we offer a complete workf...
      Speaker: Lorenz Fülle, PhD
      Sponsored By: Miltenyi Biotec
      12
      MAR 09, 2021 | 8:00 AM
      Ultrahigh-content imaging: a breath of fresh air for lung cancer research
      Ultrahigh-content imaging: a breath of fresh air for lung cancer...
      Date: March 09, 2021 Time: 8:00am (PST), 11:00am (EST) Lung cancer is one of the three most common types of cancer. Representing 85% of all lung cancers...
      Speaker: Dr. Telma Santos
      Sponsored By: Miltenyi Biotec
      13
      November 13, 2020 4:00 PM PST | November 14, 2020 8:00 AM SGT
      Fluorescent multiplexing of 3D spheroid FFPE with Roche automated stainer and reagents: assay optimization, imaging and future implications
      Fluorescent multiplexing of 3D spheroid FFPE with Roche automated...
      Three-dimensional (3D) cell culture spheroids and aggregates are preferred over monolayer cell culture due to their architectural and functional similarity to solid tumors. To study expressi...
      Speaker: Srabani Bhaumik, PhD
      Presented at: DISCOVERY Global User Symposium
      1
      November 12, 2020 9:00 AM PST | 4:00 PM GMT
      Fluorescent multiplexing of 3D spheroid FFPE with Roche automated stainer and reagents: assay optimization, imaging and future implications
      Fluorescent multiplexing of 3D spheroid FFPE with Roche automated...
      Three-dimensional (3D) cell culture spheroids and aggregates are preferred over monolayer cell culture due to their architectural and functional similarity to solid tumors. To study expressi...
      Speaker: Srabani Bhaumik, PhD
      Presented at: DISCOVERY Global User Symposium
      1
      NOV 12, 2020 | 12:00 AM
      Principles of Effective Method Development in Pathobiology Research
      Principles of Effective Method Development in Pathobiology Research
      Immunohistochemistry (IHC) is used to determine spatial relationships where we can identify the localization of target proteins in specific regions and cells of a tissue. IHC has been the go...
      Speaker: Vivian Barry, MS
      Presented at: DISCOVERY Global User Symposium
      2
      OCT 08, 2020 | 10:30 AM
      Keynote Presentation: Principles of Effective Method Development in Pathobiology Research.
      Keynote Presentation: Principles of Effective Method Development...
      Immunohistochemistry (IHC) is used to determine spatial relationships where we can identify the localization of target proteins in specific regions and cells of a tissue. IHC has been the go...
      Speaker: Vivian Barry, MS
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
      Sponsored By: Roche Diagnostics
      1
      OCT 06, 2020 | 10:00 AM
      The Art and Science of Haematology Morphology
      The Art and Science of Haematology Morphology
      DATE: October 6, 2020 TIME: 10:00am (PDT) The enumeration and analysis of blood cells with automated haematology analysers have significantly improved in recent years due to advances in cell...
      Speaker: Daniel Pelling, CSci, FIBMS, DFM
      Sponsored By: Abbott
      51 6
      SEP 23, 2020 | 2:30 PM
      Tutorials: Data Analytics
      Tutorials: Data Analytics
      Intro to high-dimensional data analysis 3:00–3:30 pm PDT Presented By: El-ad David Amir, PhD FAUST: A new interpretable machine learning approach for automated gating 3:30–4:00 p...
      Speaker: Trevor McKee, PhD , El-ad David Amir, PhD , Raphael Gottardo, PhD
      Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
      1
      SEP 22, 2020 | 11:00 AM
      A Novel Method for Quantitative  Western Blotting that Eliminates Errors from the Use of  Housekeeping Proteins
      A Novel Method for Quantitative Western Blotting that Eliminates...
      DATE: August 22, 2020 TIME: 9:00am PDT The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins...
      Speaker: Alok Tomar, PhD
      Sponsored By: Thermo Fisher Scientific
      11
      SEP 15, 2020 | 8:00 AM
      Next generation ultra high content analysis of the tumor immune microenvironment using MICS technology
      Next generation ultra high content analysis of the tumor immune...
      DATE: September 15, 2020 TIME: 08:00am PT Tumorigenesis and cancer progression are affected by the tumor immune microenvironment (TIME). The TIME not only influences the efficiency of existi...
      Speaker: Stefan Eulitz
      Sponsored By: Miltenyi Biotec
      15 2
      MAY 20, 2020 | 1:15 PM
      Flotetuzumab, an Investigational CD123 x CD3 Bispecific Dart® Protein-Induced Clustering of CD3+ T Cells & CD123+ AML Cells in Bone Marrow Biopsies Is Associated with Response to Treatment...
      Flotetuzumab, an Investigational CD123 x CD3 Bispecific Dart®...
      Background: Acute myeloid leukemia (AML) is a molecularly and clinically heterogeneous hematological malignancy. Despite recent advancements in the treatment of AML, approximately 50% of pat...
      Speaker: Jan Davidson, MD, PhD
      Presented at: Advancing Science - A Virtual Oncology Conference
      1
      MAY 06, 2020 | 1:00 PM
      Estimating the age of biological evidence recovered from a crime scene by assessing RNA degradation in the sample
      Estimating the age of biological evidence recovered from a crime...
      The forensic investigation of crime involves answering four basic questions: who, what, where, and when. Tools exist to answer questions of who, what and where, but the question when is ofte...
      Speaker: Robert W. Allen, PhD
      Presented at: Forensic Sciences Virtual Event Series 2020
      5
      APR 28, 2020 | 10:00 AM
      Measuring Single EVs and their Cargo: Sensitive and Specific Vesicle Flow Cytometry (vFCTM)
      Measuring Single EVs and their Cargo: Sensitive and Specific Vesicle...
      DATE: April 28, 2020 TIME: 10:00am PT All cells release extracellular vesicles (EVs) that can carry molecular cargo to other cells to affect their function. EVs are promising as potential bi...
      Speaker: John Nolan, PhD
      Sponsored By: Beckman Coulter Life Sciences
      10 1
    OCT 06, 2021 | 9:00 AM
    Antibody titration for flow cytometric analysis of Extracellular Vesicles: Are cell based protocols up to the task?
    Antibody titration for flow cytometric analysis of Extracellular...
    Date: October 6, 2021 Time: 9:00am (PDT), 12:00pm (EDT) ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis...
    Speaker: Desmond Pink, PhD
    Sponsored By: Miltenyi Biotec
    6 1
    SEP 29, 2021 | 10:00 AM
    Study design: How and when to use multiplexed immunofluorescence imaging in your research
    Study design: How and when to use multiplexed immunofluorescence...
    Date: September 29, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Multiplexed tissue analysis is a powerful technique that allows comparisons of cell-type locations and cell-type interactions withi...
    Speaker: Melinda Angus-Hill Ph.D.
    Sponsored By: Leica Microsystems
    5
    SEP 22, 2021 | 11:00 PM
    Failing Tethers Destabilize Neutrophil Rolling and Form Elongated Neutrophil-Derived Structures (ENDS) in the Blood Circulation
    Failing Tethers Destabilize Neutrophil Rolling and Form Elongated...
    Neutrophils are the most abundant immune cells in human blood and are crucial for host defense against bacterial and fungal infections. Neutrophils are made mainly in the bone marrow from wh...
    Speaker: Alex Marki
    Presented at: Cell Biology Virtual Event Series 2021
    1
    JUN 17, 2021 | 7:00 AM
    Cell DIVE: a robust platform for multiplexed whole slide imaging and single-cell analysis
    Cell DIVE: a robust platform for multiplexed whole slide imaging...
    DATE: June 17, 2021 TIME: 07:00am PDT Colorectal cancer is a particularly challenging disease for immunotherapy given tumor heterogeneity. Multiplexed whole slide imaging allows deep context...
    Speaker: Melinda Angus-Hill Ph.D.
    Sponsored By: Leica Microsystems
    8
    JUN 16, 2021 | 8:00 AM
    Simplicity that defies complexity - the MACSima™ Platform for ultrahigh-content imaging
    Simplicity that defies complexity - the MACSima™ Platform for...
    Date: June 16, 2021 Time: 8:00am PDT The MACSima™ Imaging Platform is the only complete solution for ultrahigh-content imaging of hundreds of markers, allowing you to understand the tu...
    Speaker: Dr. Ali Kinkhabwala , Dr. Markus Habich
    Sponsored By: Miltenyi Biotec
    7
     
     
  • JUN 02, 2021 | 9:00 AM
    Making sense out of multiplex
    Making sense out of multiplex
    The Tumor Microenvironment (TME) is a highly complex niche, with many different cell types characterized by a range of biomarkers. Clever staining protocols like Cycle Immunofluorescence, an...
    Speaker: Dave Mason, PhD
    Presented at: Immuno-Oncology Virtual Event Series 2021
    Sponsored By: Visiopharm®
    4
    JUN 02, 2021 | 8:00 AM
    Exploiting digital histology approaches to probe the pathophysiology of SARS-CoV-2 infection
    Exploiting digital histology approaches to probe the pathophysiology...
    Date: June 2, 2021 Time: 8:00am (PDT), 11:00am (EDT) In this introduction Kelly Hunter will address the first Learning Objective. He will cover i) a range of digital pathology and tissue mul...
    Speaker: Kelly Hunter, BSc Biochemistry, MSc Genomics Medicine , Dr. Graham Taylor, BSc, Ph.D
    Sponsored By: Leica Biosystems
    11 1
    MAY 26, 2021 | 9:00 AM
    Advanced Phenotyping of the Tumor Immune Microenvironment
    Advanced Phenotyping of the Tumor Immune Microenvironment
    Date: May 26, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Immunotherapy has transformed the treatment of metastatic and recurrent solid tumors but is challenged in that only a minority of patie...
    Speaker: Angela Vasaturo, Ph.D. , Fabian Schneider, Ph.D.
    Sponsored By: Ultivue
    6 1
    APR 29, 2021 | 10:00 AM
    Quantitative western blotting analysis: tips, considerations, and streamlining analysis using Invitrogen iBright Analysis Software
    Quantitative western blotting analysis: tips, considerations,...
    Date: April 29, 2021 Time: 10:00am PDT Western blotting is a fundamental application used in life science research to better understand target proteins and their function in complex systems....
    Speaker: Janaki Narahari, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    5
    APR 28, 2021 | 9:00 AM
    Combining protein proximity detection with chromogenic multiplex: Discovering the PD1/PDL1 interactions
    Combining protein proximity detection with chromogenic multiplex:...
    Proximity Ligation Assay (PLA), is a powerful technique for demonstrating the presence of 2 unique targets in direct proximity of each other in tissue sections. It allows visualization of in...
    Speaker: Thierry P.P. van den Bosch, PhD
    Presented at: DISCOVERY Global User Symposium
    3
     
     
  • APR 21, 2021 | 5:00 PM
    Immunology Researchers share their Spatial Discoveries in SARS-CoV-2 host response and in multiple sclerosis
    Immunology Researchers share their Spatial Discoveries in SARS-CoV-2...
    Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
    Speaker: Doug Brown, PhD , Susanne Krasemann, MD, PhD , Michelle Naughton, PhD
    Sponsored By: NanoString Technologies, Illumina
    5
    MAR 16, 2021 | 8:00 AM
    The latest developments for enriching and analyzing antigen- and virus-specific T cells
    The latest developments for enriching and analyzing antigen- and...
    DATE: March 09, 2021 TIME: 08:00am PST The COVID-19 pandemic has placed urgency on precise analysis of rare antigen- and virus-specific T cells. At Miltenyi Biotec, we offer a complete workf...
    Speaker: Lorenz Fülle, PhD
    Sponsored By: Miltenyi Biotec
    12
    MAR 09, 2021 | 8:00 AM
    Ultrahigh-content imaging: a breath of fresh air for lung cancer research
    Ultrahigh-content imaging: a breath of fresh air for lung cancer...
    Date: March 09, 2021 Time: 8:00am (PST), 11:00am (EST) Lung cancer is one of the three most common types of cancer. Representing 85% of all lung cancers...
    Speaker: Dr. Telma Santos
    Sponsored By: Miltenyi Biotec
    13
    November 13, 2020 4:00 PM PST | November 14, 2020 8:00 AM SGT
    Fluorescent multiplexing of 3D spheroid FFPE with Roche automated stainer and reagents: assay optimization, imaging and future implications
    Fluorescent multiplexing of 3D spheroid FFPE with Roche automated...
    Three-dimensional (3D) cell culture spheroids and aggregates are preferred over monolayer cell culture due to their architectural and functional similarity to solid tumors. To study expressi...
    Speaker: Srabani Bhaumik, PhD
    Presented at: DISCOVERY Global User Symposium
    1
    November 12, 2020 9:00 AM PST | 4:00 PM GMT
    Fluorescent multiplexing of 3D spheroid FFPE with Roche automated stainer and reagents: assay optimization, imaging and future implications
    Fluorescent multiplexing of 3D spheroid FFPE with Roche automated...
    Three-dimensional (3D) cell culture spheroids and aggregates are preferred over monolayer cell culture due to their architectural and functional similarity to solid tumors. To study expressi...
    Speaker: Srabani Bhaumik, PhD
    Presented at: DISCOVERY Global User Symposium
    1
     
     
  • NOV 12, 2020 | 12:00 AM
    Principles of Effective Method Development in Pathobiology Research
    Principles of Effective Method Development in Pathobiology Research
    Immunohistochemistry (IHC) is used to determine spatial relationships where we can identify the localization of target proteins in specific regions and cells of a tissue. IHC has been the go...
    Speaker: Vivian Barry, MS
    Presented at: DISCOVERY Global User Symposium
    2
    OCT 08, 2020 | 10:30 AM
    Keynote Presentation: Principles of Effective Method Development in Pathobiology Research.
    Keynote Presentation: Principles of Effective Method Development...
    Immunohistochemistry (IHC) is used to determine spatial relationships where we can identify the localization of target proteins in specific regions and cells of a tissue. IHC has been the go...
    Speaker: Vivian Barry, MS
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
    Sponsored By: Roche Diagnostics
    1
    OCT 06, 2020 | 10:00 AM
    The Art and Science of Haematology Morphology
    The Art and Science of Haematology Morphology
    DATE: October 6, 2020 TIME: 10:00am (PDT) The enumeration and analysis of blood cells with automated haematology analysers have significantly improved in recent years due to advances in cell...
    Speaker: Daniel Pelling, CSci, FIBMS, DFM
    Sponsored By: Abbott
    51 6
    SEP 23, 2020 | 2:30 PM
    Tutorials: Data Analytics
    Tutorials: Data Analytics
    Intro to high-dimensional data analysis 3:00–3:30 pm PDT Presented By: El-ad David Amir, PhD FAUST: A new interpretable machine learning approach for automated gating 3:30–4:00 p...
    Speaker: Trevor McKee, PhD , El-ad David Amir, PhD , Raphael Gottardo, PhD
    Presented at: 9th Annual Fluidigm Mass Cytometry Virtual Summit
    1
    SEP 22, 2020 | 11:00 AM
    A Novel Method for Quantitative  Western Blotting that Eliminates Errors from the Use of  Housekeeping Proteins
    A Novel Method for Quantitative Western Blotting that Eliminates...
    DATE: August 22, 2020 TIME: 9:00am PDT The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins...
    Speaker: Alok Tomar, PhD
    Sponsored By: Thermo Fisher Scientific
    11
     
     
  • SEP 15, 2020 | 8:00 AM
    Next generation ultra high content analysis of the tumor immune microenvironment using MICS technology
    Next generation ultra high content analysis of the tumor immune...
    DATE: September 15, 2020 TIME: 08:00am PT Tumorigenesis and cancer progression are affected by the tumor immune microenvironment (TIME). The TIME not only influences the efficiency of existi...
    Speaker: Stefan Eulitz
    Sponsored By: Miltenyi Biotec
    15 2
    MAY 20, 2020 | 1:15 PM
    Flotetuzumab, an Investigational CD123 x CD3 Bispecific Dart® Protein-Induced Clustering of CD3+ T Cells & CD123+ AML Cells in Bone Marrow Biopsies Is Associated with Response to Treatment...
    Flotetuzumab, an Investigational CD123 x CD3 Bispecific Dart®...
    Background: Acute myeloid leukemia (AML) is a molecularly and clinically heterogeneous hematological malignancy. Despite recent advancements in the treatment of AML, approximately 50% of pat...
    Speaker: Jan Davidson, MD, PhD
    Presented at: Advancing Science - A Virtual Oncology Conference
    1
    MAY 06, 2020 | 1:00 PM
    Estimating the age of biological evidence recovered from a crime scene by assessing RNA degradation in the sample
    Estimating the age of biological evidence recovered from a crime...
    The forensic investigation of crime involves answering four basic questions: who, what, where, and when. Tools exist to answer questions of who, what and where, but the question when is ofte...
    Speaker: Robert W. Allen, PhD
    Presented at: Forensic Sciences Virtual Event Series 2020
    5
    APR 28, 2020 | 10:00 AM
    Measuring Single EVs and their Cargo: Sensitive and Specific Vesicle Flow Cytometry (vFCTM)
    Measuring Single EVs and their Cargo: Sensitive and Specific Vesicle...
    DATE: April 28, 2020 TIME: 10:00am PT All cells release extracellular vesicles (EVs) that can carry molecular cargo to other cells to affect their function. EVs are promising as potential bi...
    Speaker: John Nolan, PhD
    Sponsored By: Beckman Coulter Life Sciences
    10 1
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