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    Research and Development

    Research and Development: innovative activities performed by corporations or governments for the creation, discovery and advancement of services and products.

    Webinars

    • OCT 14, 2021 | 9:00 AM
      Session 3: Simple steps to publication-quality western blots
      Session 3: Simple steps to publication-quality western blots
      Date: October 14, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe...
      Speaker: Kelli Feather-Henigan
      Presented at: Webinar series: Simple steps to publication-quality western blots
      Sponsored By: Thermo Fisher Scientific
      28
      OCT 14, 2021 | 8:00 AM
      Ensure reproducibility in your B cell analysis
      Ensure reproducibility in your B cell analysis
      Date: October 14, 2021 Time: 8:00am (PDT), 11:00am (EDT) The primary role of B cells is to secrete antibodies, boosting our adaptive immune responses, making them an essential part of our im...
      Speaker: Urmi Roy, PhD , Dr. Susanne Bethke
      Sponsored By: Miltenyi Biotec
      15
      OCT 13, 2021 | 11:55 PM
      Low-Dose mRNA-1273 COVID-19 Vaccine Generates Durable Memory Enhanced by Cross-reactive T Cells
      Low-Dose mRNA-1273 COVID-19 Vaccine Generates Durable Memory Enhanced...
      Vaccination and infection are two different paths to immunity. Understanding human immune responses to SARS-CoV-2 RNA vaccines is of interest for a panoply of reasons. mRNA vaccines have dem...
      Speaker: Daniela Weiskopf, PhD
      Presented at: Coronavirus Series
      1
      OCT 13, 2021 | 4:30 PM
      Delivering Insights by Analyzing Metagenomic NGS Data
      Delivering Insights by Analyzing Metagenomic NGS Data
      In this session we will use a set of reads enriched for viral sequences and sequences from wastewater treatment plants. Specifically the aim is to assemble whole SARS-CoV-2 genomes from such...
      Speaker: Patrick Ettenhuber, PhD
      Presented at: Coronavirus Series
      Sponsored By: QIAGEN
      1
      OCT 13, 2021 | 3:00 PM
      Accelerating SARS-CoV-2 Genomic Surveillance with High-throughput NGS
      Accelerating SARS-CoV-2 Genomic Surveillance with High-throughput...
      Since late 2020, several prominent SARS-CoV-2 variants of concern have emerged, harboring specific mutations which increase viral transmissibility Delta variant) and which appear to reduce t...
      Speaker: James Caffrey, PhD, MBA
      Presented at: Coronavirus Series
      Sponsored By: QIAGEN
      1
      OCT 13, 2021 | 1:30 PM
      SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping​
      SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping​
      This presentation “SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping” will focus on strategies for the testing and detection of SARS-CoV-2, including multiplexe...
      Speaker: Manohar R. Furtado, PhD
      Presented at: Coronavirus Series
      Sponsored By: Thermo Fisher Scientific
      14
      OCT 13, 2021 | 1:30 PM
      Panel Presentation: Advantage of Absolute Quantification of Digital PCR in Wastewater Monitoring QIAGEN's Workflow Solutions for SARS-CoV-2 Surveillance
      Panel Presentation: Advantage of Absolute Quantification of Digital...
      One significant challenge with SARS-CoV-2 wastewater monitoring is the lack of optimized and standardized sample processing steps, from sample collection to RNA detection. Viral concentratio...
      Speaker: Dominic O'Neil, MS, MBA , Colin Donohoe, PhD
      Presented at: Coronavirus Series
      Sponsored By: QIAGEN
      1
      OCT 13, 2021 | 2:00 PM
      C.E. CREDITS
      The Patient with Venous Thrombosis - Practical Points for the Laboratorian and the Clinician
      The Patient with Venous Thrombosis - Practical Points for the...
      Learn 22 key clinical insights on diagnosing and managing VTE, avoiding pitfalls in imaging, labs, and anticoagulation decisions....
      Speaker: Stephan Moll, MD
      Presented at: Stago EdVantage Virtual University
      Sponsored By: Diagnostica Stago, Inc.
      6
      OCT 13, 2021 | 10:30 AM
      Altered MicroRNA Expression in COVID-19 Patients Accurately Identifies of SARS-CoV-2 Infection
      Altered MicroRNA Expression in COVID-19 Patients Accurately Identifies...
      By examining the host response to SARS-CoV-2 infection we gain valuable insights into viral pathogenesis and COVID-19 progression. MicroRNAs (miRNAs), a class of small (18-22nt), non-coding...
      Speaker: Ryan Farr, PhD
      Presented at: Coronavirus Series
      Sponsored By: QIAGEN
      1
      OCT 13, 2021 | 10:00 AM
      Overcoming sequence artifacts to fuel clinically relevant NGS applications
      Overcoming sequence artifacts to fuel clinically relevant NGS...
      Date: October 13, 2021 Time: 10:00am (PDT), 01:00pm (EDT) Clinically-relevant applications, such as somatic variant detection, continue to push the bounds...
      Speaker: Zane Jaafar, Ph.D
      Sponsored By: Watchmaker Genomics
      27
      OCT 13, 2021 | 7:00 AM
      Session 2: Simple steps to publication-quality western blots
      Session 2: Simple steps to publication-quality western blots
      Date: October 13, 2021 Time: 7:00am (PDT), 10:00am (EDT) Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target...
      Speaker: Caleb Shearrow
      Presented at: Webinar series: Simple steps to publication-quality western blots
      Sponsored By: Thermo Fisher Scientific
      31 1
      OCT 13, 2021 | 6:00 AM
      Keynote Presentation: Discovery and Biology of Protective SARS-CoV-2 Human Monoclonal Antibodies
      Keynote Presentation: Discovery and Biology of Protective SARS-CoV-2...
      We’ll discuss the rapid discovery efforts to identify human monoclonal antibody drugs that protect against COVID-19. We will review the technologies used to find antibodies, the optima...
      Speaker: James Crowe, MD
      Presented at: Coronavirus Series
      26
      OCT 13, 2021 | 12:00 AM
      SARS-CoV-2 Evolution: Addition by Subtraction
      SARS-CoV-2 Evolution: Addition by Subtraction
      SARS-CoV-2 is an emergent virus. Specifically how it will adapt to human hosts and populations with growing immunity is unknown. Coronaviruses acquire substitutions more slowly than other RN...
      Speaker: Kevin R. McCarthy, PhD
      Presented at: Coronavirus Series
      0
      OCT 13, 2021 | 12:00 AM
      Rapid and Inexpensive Whole-Genome Sequencing of SARS-CoV-2 Using the "Midnight" Panel
      Rapid and Inexpensive Whole-Genome Sequencing of SARS-CoV-2 Using...
      I will discuss the development of the “Midnight” panel for faster, simpler, and less expensive SARS-CoV-2 genome sequencing. We show that using a multiplexed set of primers in co...
      Speaker: Nikki Freed, PhD
      Presented at: Coronavirus Series
      2
      OCT 12, 2021 | 9:00 AM
      An overview and demonstration of SCIEX Biologics Explorer software for biologics characterization
      An overview and demonstration of SCIEX Biologics Explorer software...
      Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
      Speaker: Mona Hamada, Ph.D. , Stephen Sciuto, Ph.D.
      Sponsored By: SCIEX
      6
      OCT 12, 2021 | 8:00 AM
      The growing concern of off-target effects: How to measure and minimize off-target effects in your genome editing experiments.
      The growing concern of off-target effects: How to measure and...
      Date: October 12, 2021 Time: 8:00am (PDT), 11:00am (EDT) CRISPR-Cas9 is a nuclease-based genome editing system which has seen exponential growth in adoption with broad applications from basi...
      Speaker: Pandhita Pochanard
      Sponsored By: Thermo Fisher Scientific/Gibco
      30
      OCT 12, 2021 | 6:00 AM
      MALDI Biotyper® sirius and resistance detection
      MALDI Biotyper® sirius and resistance detection
      Date: October 12, 2021 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) Dr. Diana Kalinina shall introduce the MALDI Biotyper ® sirius platform and elaborate the already available as well...
      Speaker: Diana Kalinina, PhD , Gerald Larrouy-Maumus, PhD
      Sponsored By: Bruker Microbiology & Infection Diagnostics
      10
      OCT 07, 2021 | 1:30 PM
      Harnessing Insight from Real-world Oncology Cases and QIAGEN Knowledge to Annotate Variants and Assess Prevalence: Introducing a New Somatic Database-Our Best Kept Secret
      Harnessing Insight from Real-world Oncology Cases and QIAGEN Knowledge...
      Competing to offer a comprehensive genomic profiling service for solid or hematology oncology tumor samples is challenging, especially as panels increase in size and complexity. QIAGEN Clini...
      Speaker: Beate Litzenburger, Ph.D.
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
      Sponsored By: QIAGEN
      0
      OCT 07, 2021 | 12:00 PM
      Panel Presentation: Oncology Variant Interpretation Just Got More Precise
      Panel Presentation: Oncology Variant Interpretation Just Got More...
      Across oncology applications, from research to molecular testing and pharmaceutical development, the ability to identify potentially actionable genetic alterations and exploit the molecular...
      Speaker: Beate Litzenburger, Ph.D. , Sheryl Elkin, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
      Sponsored By: QIAGEN
      2
      OCT 07, 2021 | 10:00 AM
      Optimizing tumor study performance:  Evaluating the B-NDG & NSGâ„¢ models in PDX and CDX tumor studies.
      Optimizing tumor study performance: Evaluating the B-NDG & NSGâ„¢...
      Date: October 07, 2021 Time: 10:00am (PDT), 1:00pm (EDT) It's estimated than less than 10% of investigational anti-cancer drugs successfully achieve FDA approval for clinical use. This i...
      Speaker: Viktoria Hyddmark
      Sponsored By: Envigo
      3 1
      OCT 06, 2021 | 10:30 AM
      Early Identification of Target Candidates for Pancreatic Adenocarcinoma Immunotherapy
      Early Identification of Target Candidates for Pancreatic Adenocarcinoma...
      Effective immunotherapy of pancreatic ductal adenocarcinoma (PDAC) is hampered by the lack of suitable tumor-specific antigens. To address this challenge, we have combined high-throughput fl...
      Speaker: Olaf Hardt, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
      Sponsored By: Miltenyi Biotec
      1
      OCT 06, 2021 | 9:00 AM
      Antibody titration for flow cytometric analysis of Extracellular Vesicles: Are cell based protocols up to the task?
      Antibody titration for flow cytometric analysis of Extracellular...
      Date: October 6, 2021 Time: 9:00am (PDT), 12:00pm (EDT) ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis...
      Speaker: Desmond Pink, PhD
      Sponsored By: Miltenyi Biotec
      6 1
      OCT 06, 2021 | 7:30 AM
      Keynote Presentation: Why History Matters When Addressing Health Disparities
      Keynote Presentation: Why History Matters When Addressing Health...
      There is considerable research being conducting on the topic of health disparities. It is important to understand why these health disparities exist as well as what is contributing to so man...
      Speaker: Angelo Moore, PhD, RN, NE-BC
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
      1
      OCT 06, 2021 | 12:00 AM
      Exploring Breast Cancer Health Disparities in African American Women
      Exploring Breast Cancer Health Disparities in African American...
      African American women face a lower risk of being diagnosed with breast cancer as compared to Caucasian-American women, yet they paradoxically face an increased breast cancer mortality hazar...
      Speaker: Checo J. Rorie, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
      1 1
    OCT 14, 2021 | 9:00 AM
    Session 3: Simple steps to publication-quality western blots
    Session 3: Simple steps to publication-quality western blots
    Date: October 14, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe...
    Speaker: Kelli Feather-Henigan
    Presented at: Webinar series: Simple steps to publication-quality western blots
    Sponsored By: Thermo Fisher Scientific
    28
    OCT 14, 2021 | 8:00 AM
    Ensure reproducibility in your B cell analysis
    Ensure reproducibility in your B cell analysis
    Date: October 14, 2021 Time: 8:00am (PDT), 11:00am (EDT) The primary role of B cells is to secrete antibodies, boosting our adaptive immune responses, making them an essential part of our im...
    Speaker: Urmi Roy, PhD , Dr. Susanne Bethke
    Sponsored By: Miltenyi Biotec
    15
    OCT 13, 2021 | 11:55 PM
    Low-Dose mRNA-1273 COVID-19 Vaccine Generates Durable Memory Enhanced by Cross-reactive T Cells
    Low-Dose mRNA-1273 COVID-19 Vaccine Generates Durable Memory Enhanced...
    Vaccination and infection are two different paths to immunity. Understanding human immune responses to SARS-CoV-2 RNA vaccines is of interest for a panoply of reasons. mRNA vaccines have dem...
    Speaker: Daniela Weiskopf, PhD
    Presented at: Coronavirus Series
    1
    OCT 13, 2021 | 4:30 PM
    Delivering Insights by Analyzing Metagenomic NGS Data
    Delivering Insights by Analyzing Metagenomic NGS Data
    In this session we will use a set of reads enriched for viral sequences and sequences from wastewater treatment plants. Specifically the aim is to assemble whole SARS-CoV-2 genomes from such...
    Speaker: Patrick Ettenhuber, PhD
    Presented at: Coronavirus Series
    Sponsored By: QIAGEN
    1
    OCT 13, 2021 | 3:00 PM
    Accelerating SARS-CoV-2 Genomic Surveillance with High-throughput NGS
    Accelerating SARS-CoV-2 Genomic Surveillance with High-throughput...
    Since late 2020, several prominent SARS-CoV-2 variants of concern have emerged, harboring specific mutations which increase viral transmissibility Delta variant) and which appear to reduce t...
    Speaker: James Caffrey, PhD, MBA
    Presented at: Coronavirus Series
    Sponsored By: QIAGEN
    1
     
     
  • OCT 13, 2021 | 1:30 PM
    SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping​
    SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping​
    This presentation “SARS-CoV-2 Related Workflow and Solutions: Detection and Genotyping” will focus on strategies for the testing and detection of SARS-CoV-2, including multiplexe...
    Speaker: Manohar R. Furtado, PhD
    Presented at: Coronavirus Series
    Sponsored By: Thermo Fisher Scientific
    14
    OCT 13, 2021 | 1:30 PM
    Panel Presentation: Advantage of Absolute Quantification of Digital PCR in Wastewater Monitoring QIAGEN's Workflow Solutions for SARS-CoV-2 Surveillance
    Panel Presentation: Advantage of Absolute Quantification of Digital...
    One significant challenge with SARS-CoV-2 wastewater monitoring is the lack of optimized and standardized sample processing steps, from sample collection to RNA detection. Viral concentratio...
    Speaker: Dominic O'Neil, MS, MBA , Colin Donohoe, PhD
    Presented at: Coronavirus Series
    Sponsored By: QIAGEN
    1
    OCT 13, 2021 | 2:00 PM
    C.E. CREDITS
    The Patient with Venous Thrombosis - Practical Points for the Laboratorian and the Clinician
    The Patient with Venous Thrombosis - Practical Points for the...
    Learn 22 key clinical insights on diagnosing and managing VTE, avoiding pitfalls in imaging, labs, and anticoagulation decisions....
    Speaker: Stephan Moll, MD
    Presented at: Stago EdVantage Virtual University
    Sponsored By: Diagnostica Stago, Inc.
    6
    OCT 13, 2021 | 10:30 AM
    Altered MicroRNA Expression in COVID-19 Patients Accurately Identifies of SARS-CoV-2 Infection
    Altered MicroRNA Expression in COVID-19 Patients Accurately Identifies...
    By examining the host response to SARS-CoV-2 infection we gain valuable insights into viral pathogenesis and COVID-19 progression. MicroRNAs (miRNAs), a class of small (18-22nt), non-coding...
    Speaker: Ryan Farr, PhD
    Presented at: Coronavirus Series
    Sponsored By: QIAGEN
    1
    OCT 13, 2021 | 10:00 AM
    Overcoming sequence artifacts to fuel clinically relevant NGS applications
    Overcoming sequence artifacts to fuel clinically relevant NGS...
    Date: October 13, 2021 Time: 10:00am (PDT), 01:00pm (EDT) Clinically-relevant applications, such as somatic variant detection, continue to push the bounds...
    Speaker: Zane Jaafar, Ph.D
    Sponsored By: Watchmaker Genomics
    27
     
     
  • OCT 13, 2021 | 7:00 AM
    Session 2: Simple steps to publication-quality western blots
    Session 2: Simple steps to publication-quality western blots
    Date: October 13, 2021 Time: 7:00am (PDT), 10:00am (EDT) Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target...
    Speaker: Caleb Shearrow
    Presented at: Webinar series: Simple steps to publication-quality western blots
    Sponsored By: Thermo Fisher Scientific
    31 1
    OCT 13, 2021 | 6:00 AM
    Keynote Presentation: Discovery and Biology of Protective SARS-CoV-2 Human Monoclonal Antibodies
    Keynote Presentation: Discovery and Biology of Protective SARS-CoV-2...
    We’ll discuss the rapid discovery efforts to identify human monoclonal antibody drugs that protect against COVID-19. We will review the technologies used to find antibodies, the optima...
    Speaker: James Crowe, MD
    Presented at: Coronavirus Series
    26
    OCT 13, 2021 | 12:00 AM
    SARS-CoV-2 Evolution: Addition by Subtraction
    SARS-CoV-2 Evolution: Addition by Subtraction
    SARS-CoV-2 is an emergent virus. Specifically how it will adapt to human hosts and populations with growing immunity is unknown. Coronaviruses acquire substitutions more slowly than other RN...
    Speaker: Kevin R. McCarthy, PhD
    Presented at: Coronavirus Series
    0
    OCT 13, 2021 | 12:00 AM
    Rapid and Inexpensive Whole-Genome Sequencing of SARS-CoV-2 Using the "Midnight" Panel
    Rapid and Inexpensive Whole-Genome Sequencing of SARS-CoV-2 Using...
    I will discuss the development of the “Midnight” panel for faster, simpler, and less expensive SARS-CoV-2 genome sequencing. We show that using a multiplexed set of primers in co...
    Speaker: Nikki Freed, PhD
    Presented at: Coronavirus Series
    2
    OCT 12, 2021 | 9:00 AM
    An overview and demonstration of SCIEX Biologics Explorer software for biologics characterization
    An overview and demonstration of SCIEX Biologics Explorer software...
    Date: October 12, 2021 Time: 9:00am (PDT), 12:00pm (EDT) SCIEX’s next-generation Biologics Explorer software is an innovative platform for the comprehensive and deep characterization o...
    Speaker: Mona Hamada, Ph.D. , Stephen Sciuto, Ph.D.
    Sponsored By: SCIEX
    6
     
     
  • OCT 12, 2021 | 8:00 AM
    The growing concern of off-target effects: How to measure and minimize off-target effects in your genome editing experiments.
    The growing concern of off-target effects: How to measure and...
    Date: October 12, 2021 Time: 8:00am (PDT), 11:00am (EDT) CRISPR-Cas9 is a nuclease-based genome editing system which has seen exponential growth in adoption with broad applications from basi...
    Speaker: Pandhita Pochanard
    Sponsored By: Thermo Fisher Scientific/Gibco
    30
    OCT 12, 2021 | 6:00 AM
    MALDI Biotyper® sirius and resistance detection
    MALDI Biotyper® sirius and resistance detection
    Date: October 12, 2021 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) Dr. Diana Kalinina shall introduce the MALDI Biotyper ® sirius platform and elaborate the already available as well...
    Speaker: Diana Kalinina, PhD , Gerald Larrouy-Maumus, PhD
    Sponsored By: Bruker Microbiology & Infection Diagnostics
    10
    OCT 07, 2021 | 1:30 PM
    Harnessing Insight from Real-world Oncology Cases and QIAGEN Knowledge to Annotate Variants and Assess Prevalence: Introducing a New Somatic Database-Our Best Kept Secret
    Harnessing Insight from Real-world Oncology Cases and QIAGEN Knowledge...
    Competing to offer a comprehensive genomic profiling service for solid or hematology oncology tumor samples is challenging, especially as panels increase in size and complexity. QIAGEN Clini...
    Speaker: Beate Litzenburger, Ph.D.
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
    Sponsored By: QIAGEN
    0
    OCT 07, 2021 | 12:00 PM
    Panel Presentation: Oncology Variant Interpretation Just Got More Precise
    Panel Presentation: Oncology Variant Interpretation Just Got More...
    Across oncology applications, from research to molecular testing and pharmaceutical development, the ability to identify potentially actionable genetic alterations and exploit the molecular...
    Speaker: Beate Litzenburger, Ph.D. , Sheryl Elkin, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
    Sponsored By: QIAGEN
    2
    OCT 07, 2021 | 10:00 AM
    Optimizing tumor study performance:  Evaluating the B-NDG & NSGâ„¢ models in PDX and CDX tumor studies.
    Optimizing tumor study performance: Evaluating the B-NDG & NSGâ„¢...
    Date: October 07, 2021 Time: 10:00am (PDT), 1:00pm (EDT) It's estimated than less than 10% of investigational anti-cancer drugs successfully achieve FDA approval for clinical use. This i...
    Speaker: Viktoria Hyddmark
    Sponsored By: Envigo
    3 1
     
     
  • OCT 06, 2021 | 10:30 AM
    Early Identification of Target Candidates for Pancreatic Adenocarcinoma Immunotherapy
    Early Identification of Target Candidates for Pancreatic Adenocarcinoma...
    Effective immunotherapy of pancreatic ductal adenocarcinoma (PDAC) is hampered by the lack of suitable tumor-specific antigens. To address this challenge, we have combined high-throughput fl...
    Speaker: Olaf Hardt, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
    Sponsored By: Miltenyi Biotec
    1
    OCT 06, 2021 | 9:00 AM
    Antibody titration for flow cytometric analysis of Extracellular Vesicles: Are cell based protocols up to the task?
    Antibody titration for flow cytometric analysis of Extracellular...
    Date: October 6, 2021 Time: 9:00am (PDT), 12:00pm (EDT) ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis...
    Speaker: Desmond Pink, PhD
    Sponsored By: Miltenyi Biotec
    6 1
    OCT 06, 2021 | 7:30 AM
    Keynote Presentation: Why History Matters When Addressing Health Disparities
    Keynote Presentation: Why History Matters When Addressing Health...
    There is considerable research being conducting on the topic of health disparities. It is important to understand why these health disparities exist as well as what is contributing to so man...
    Speaker: Angelo Moore, PhD, RN, NE-BC
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
    1
    OCT 06, 2021 | 12:00 AM
    Exploring Breast Cancer Health Disparities in African American Women
    Exploring Breast Cancer Health Disparities in African American...
    African American women face a lower risk of being diagnosed with breast cancer as compared to Caucasian-American women, yet they paradoxically face an increased breast cancer mortality hazar...
    Speaker: Checo J. Rorie, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
    1 1
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