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Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
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SEP 08, 2021 | 6:00 AM PDT
Luminex's xMAP® Technology enables the evaluation of up to 500 analytes simultaneously in a single well, known as multiplexing. xMAP Technology uses color-coded microspheres as the substrate...
Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample. However, in recent years the reprod...OCT 10, 2018 | 6:00 AM PDT
The 6th Annual Event in the Cancer Research & Oncology Week Virtual Event Series will take place October 2018....OCT 03, 2018 | 6:00 AM PDT
Labroots invites you to the 1st Annual Event in the CRISPR Virtual Event Series! With continued adoption and integration in research labs...SEP 17, 2018
Improve your protein research knowledge by joining our 7 Steps of Protein virtual eventThe 3rd annual 7 Steps of Protein virtual event is taking place around the world on Tuesday, Septemb...
Experience the latest advances in protein expression at our 3rd annual virtual event In 2017, the virtual event had 2,400 international participants in over 80 countries! Th...OCT 11, 2017 | 6:00 AM PDT
The 5th Annual Event in the Cancer Research & Oncology Week Virtual Event Series is now On-Demand!...APR 10, 2017 | 6:00 AM PDT
The Protein and cell analysis education site is a free-access destination where you can learn more about applications and techniques related to protein gel chemistries, western detection, mas...JAN 25, 2017 | 5:00 AM PST
The Abcam virtual exhibition is temporarily closed. It will re-open shortly. Please check back October 25th. All webinars will be available for viewing at that time.Get the su...
