Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
JUN 09, 2021
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Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
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Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
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MAY 27, 2021
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Date: May 27, 2021 Time: 8:00am (PDT), 11:00am (EDT) With social distancing measures still enforced in large parts of the world, many researchers must minimize time in the lab where they may...
MAY 25, 2021
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MAY 25, 2021
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Date: May 25, 2021 Time: 8:00am (PDT) Reliable expression of recombinant proteins in human, CHO, or insect cells is essential for many aspects of biomedical research and drug development, bu...
MAY 13, 2021
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MAY 13, 2021
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MAY 11, 2021
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MAY 11, 2021
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Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
APR 29, 2021
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APR 29, 2021
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Date: April 29, 2021 Time: 10:00am PDT Western blotting is a fundamental application used in life science research to better understand target proteins and their function in complex systems....
APR 06, 2021
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APR 06, 2021
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DATE: April 6, 2021 TIME: 10:00am PDT Accurate and efficient data analysis of protein targets is an integral part of the gel and western blot imaging workflow. The Invitrogen iBright Analysi...
MAR 31, 2021
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MAR 31, 2021
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FEB 24, 2021
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DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
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As many parts of the northern hemisphere move into the colder months and indoors, SARS CoV-2 has begun a second infection wave that has exceeded the first surge of cases in many global regio...
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DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
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Every patient is different and so is every tumor! The term “personalized oncology” today is largely associated with sequencing approaches. Current publications proof more and mor...
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For more than a century, breakthroughs in biological sciences have relied on the ability to study cells outside of respective organisms. While majority of cell culturing is still performed u...
SEP 22, 2020
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SEP 22, 2020
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DATE: August 22, 2020 TIME: 9:00am PDT The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins...
SEP 03, 2020
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SEP 03, 2020
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DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
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DATE: July 8, 2020 TIME: 8:00am PDT In this webinar, discover how targeted protein degradation is being used to drive drug discovery. Hosted by Gary Allenby, Chief Executive Officer of Aurel...
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DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...
SEP 23, 2020
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For more than a century, breakthroughs in biological sciences have relied on the ability to study cells outside of respective organisms. While majority of cell culturing is still performed u...
SEP 22, 2020
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SEP 22, 2020
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DATE: August 22, 2020 TIME: 9:00am PDT The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins...
SEP 03, 2020
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SEP 03, 2020
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DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
JUL 08, 2020
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JUL 08, 2020
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DATE: July 8, 2020 TIME: 8:00am PDT In this webinar, discover how targeted protein degradation is being used to drive drug discovery. Hosted by Gary Allenby, Chief Executive Officer of Aurel...
JUN 18, 2020
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JUN 18, 2020
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DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...