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Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
OCT 14, 2021 | 9:00 AM
Date: October 14, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe...
OCT 13, 2021 | 3:00 PM
SARS-CoV-2 testing is now facing increasing challenges in the face of an ongoing pandemic with the emergence of new variants and waning vaccine immunity. Increasing evidence supports the cor...
OCT 13, 2021 | 7:00 AM
Date: October 13, 2021 Time: 7:00am (PDT), 10:00am (EDT) Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target...
OCT 12, 2021 | 5:00 PM
Date: October 12, 2021 Time: 5:00pm (PDT), 8:00pm (EDT) 222 Learning Objectives 222 222 222 Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved...
OCT 05, 2021 | 7:00 AM
Date: October 05, 2021 Time: 7:00am (PDT), 10:00am (EDT) After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next...
SEP 23, 2021 | 9:00 AM
Date: September 23, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Western blotting and other immunological protein-analytical methods are widely used for detecting and (semi-)quantifying specific p...
SEP 22, 2021 | 12:00 PM
While providing indispensable insight into immune processes, the live cell imaging of immune cells poses unique challenges due to their non-adherent nature. Suspension cells such as immune c...
JUN 09, 2021 | 7:00 AM
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUN 08, 2021 | 9:00 AM
Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
MAY 27, 2021 | 8:00 AM
Date: May 27, 2021 Time: 8:00am (PDT), 11:00am (EDT) With social distancing measures still enforced in large parts of the world, many researchers must minimize time in the lab where they may...
MAY 25, 2021 | 8:00 AM
Date: May 25, 2021 Time: 8:00am (PDT) Reliable expression of recombinant proteins in human, CHO, or insect cells is essential for many aspects of biomedical research and drug development, bu...
MAY 13, 2021 | 11:00 AM
Date: May 13, 2021 Time: 11:00am PST Dr. Dattwyler will share his extensive experience on the clinical diagnosis and treatment of Lyme disease including a brief history of Lyme Disease and h...
MAY 11, 2021 | 10:00 AM
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
APR 29, 2021 | 10:00 AM
Date: April 29, 2021 Time: 10:00am PDT Western blotting is a fundamental application used in life science research to better understand target proteins and their function in complex systems....
APR 06, 2021 | 10:00 AM
DATE: April 6, 2021 TIME: 10:00am PDT Accurate and efficient data analysis of protein targets is an integral part of the gel and western blot imaging workflow. The Invitrogen iBright Analysi...
MAR 31, 2021 | 8:00 AM
Date: March 31, 2021 Time: 08:00am PST CytoFLEX SRT has enabled researcher lead self-service sorting. The need for a cell sorting expert to setup and maintain the sorter status is now necess...
FEB 24, 2021 | 10:00 AM
DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
NOV 12, 2020 | 12:00 PM
As many parts of the northern hemisphere move into the colder months and indoors, SARS CoV-2 has begun a second infection wave that has exceeded the first surge of cases in many global regio...
OCT 08, 2020 | 7:00 AM
DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
JUN 09, 2021 | 7:00 AM
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUN 08, 2021 | 9:00 AM
Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
MAY 27, 2021 | 8:00 AM
Date: May 27, 2021 Time: 8:00am (PDT), 11:00am (EDT) With social distancing measures still enforced in large parts of the world, many researchers must minimize time in the lab where they may...
MAY 25, 2021 | 8:00 AM
Date: May 25, 2021 Time: 8:00am (PDT) Reliable expression of recombinant proteins in human, CHO, or insect cells is essential for many aspects of biomedical research and drug development, bu...
MAY 13, 2021 | 11:00 AM
Date: May 13, 2021 Time: 11:00am PST Dr. Dattwyler will share his extensive experience on the clinical diagnosis and treatment of Lyme disease including a brief history of Lyme Disease and h...
MAY 11, 2021 | 10:00 AM
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
APR 29, 2021 | 10:00 AM
Date: April 29, 2021 Time: 10:00am PDT Western blotting is a fundamental application used in life science research to better understand target proteins and their function in complex systems....
APR 06, 2021 | 10:00 AM
DATE: April 6, 2021 TIME: 10:00am PDT Accurate and efficient data analysis of protein targets is an integral part of the gel and western blot imaging workflow. The Invitrogen iBright Analysi...
MAR 31, 2021 | 8:00 AM
Date: March 31, 2021 Time: 08:00am PST CytoFLEX SRT has enabled researcher lead self-service sorting. The need for a cell sorting expert to setup and maintain the sorter status is now necess...
FEB 24, 2021 | 10:00 AM
DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
NOV 12, 2020 | 12:00 PM
As many parts of the northern hemisphere move into the colder months and indoors, SARS CoV-2 has begun a second infection wave that has exceeded the first surge of cases in many global regio...
OCT 08, 2020 | 7:00 AM
DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...