Western Blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
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MAY 16, 2019 04:00 PM CESTAdd to Calendar Select one of the following:
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MAY 16, 2019 04:00 PM CESTAdd to Calendar Select one of the following:
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DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...Speaker: Rita Colwell, PhD , Christiane Honisch, PhDSponsored By: EppendorfMAY 23, 2019 09:00 AM PDTAdd to Calendar Select one of the following:
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MAY 23, 2019 09:00 AM PDTAdd to Calendar Select one of the following:
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DATE: May 23, 2019TIME: 9:00am PDT, 12:00pm EDT Although mesenchymal stem/stromal cells (MSCs) chondrogenic differentiation has been thoroughly investigated...Speaker: Melika Sarem, PhDSponsored By: Thermo Fisher Scientific/GibcoJUN 05, 2019 05:00 PM CESTAdd to Calendar Select one of the following:
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JUN 05, 2019 05:00 PM CESTAdd to Calendar Select one of the following:
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DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...Speaker: Jessica Wagener, PhDSponsored By: EppendorfAPR 09, 2019 09:00 AM PDTC.E. CREDITS
APR 09, 2019 09:00 AM PDTDATE: April 9, 2019TIME: 9:00 AM PDTWe will provide a general introduction to the Single-Cell Western Technology (SCW) and then present data from 2 ongoing projects from the Col...Speaker: Noemi Kedei, MD , Kelly Gardner, Ph.D.Sponsored By: ProteinSimpleDEC 20, 2018 09:00 AM CSTC.E. CREDITS
DEC 20, 2018 09:00 AM CSTDATE: December 20, 2018TIME: 09:00am CST, 10:00am JST Host cell protein (HCP) is a significant class of process-related impurity that needs to be removed to adequatel...Speaker: Fengqiang Wang, PhDSponsored By: GE Healthcare Life SciencesDEC 18, 2018 07:00 AM PSTC.E. CREDITS
DEC 18, 2018 07:00 AM PSTDATE: December 18,2018TIME: 07:00am PST, 10:00am EST, 15:00pm GMT Host cell protein (HCP) is a significant class of process-related impurity that needs to be removed to...Speaker: Fengqiang Wang, PhDSponsored By: GE Healthcare Life SciencesDEC 13, 2018 09:00 AM PSTC.E. CREDITS
DEC 13, 2018 09:00 AM PSTDATE: December 13 15,2018TIME: 09:00am PST, 12:00pm EST Breast cancers are classified into three main subtypes according to their receptor status: estrogen receptor-positive...Speaker: Hillary Stires, PhDSponsored By: Thermo Fisher Scientific/GibcoOCT 11, 2018 01:30 PM PDTC.E. CREDITS
OCT 11, 2018 01:30 PM PDTAs the most common female malignancy, breast cancer is the most likely reason that a woman will die of cancer around the world. Breast cancer mortality has dropped in the U.S. by 35% since 19...Speaker: Benjamin Anderson, MDPresented At:Cancer Research & Oncology 2018OCT 11, 2018 12:00 PM PDTC.E. CREDITS
OCT 11, 2018 12:00 PM PDTLung cancer is the leading cause of cancer-related mortality worldwide. Large-scale sequencing studies have revealed the complex genomic landscape of NSCLC and genomic differences between lun...Speaker: Nicholas McGranahan, PhDPresented At:Cancer Research & Oncology 2018OCT 11, 2018 10:30 AM PDTC.E. CREDITS
OCT 11, 2018 10:30 AM PDTTwo projects looking at novel approaches to targeting inflammatory breast cancer will be presented. Inflammatory breast cancer (IBC) is a unique, understudied, and most lethal subtype account...Speaker: Kevin Williams, PhDPresented At:Cancer Research & Oncology 2018OCT 11, 2018 09:00 AM PDTC.E. CREDITS
OCT 11, 2018 09:00 AM PDTThe oncogenic transcription factor c-MYC (MYC) is deregulated, and often overexpressed, in more than 50% of cancers. MYC deregulation is associated with poor prognosis and aggressive disease,...Speaker: Jason De Melo, PhDPresented At:Cancer Research & Oncology 2018OCT 11, 2018 07:30 AM PDTC.E. CREDITS
OCT 11, 2018 07:30 AM PDTIn the past two decades a small number of infrequently dividing cells have been proposed as the source of multi-drug resistance during cancer treatment. These cells identified by their expres...Speaker: Krastan Blagoev, PhDPresented At:Cancer Research & Oncology 2018OCT 11, 2018 06:00 AM PDTC.E. CREDITS
OCT 11, 2018 06:00 AM PDTPacBio Sequencing simultaneously provides long sequence reads, high consensus accuracy, minimal sequence bias, and methylation detection. I will highlight new advances and updates on applying...Speaker: Jonas Korlach, PhDPresented At:Cancer Research & Oncology 2018OCT 10, 2018 12:00 PM PDTC.E. CREDITS
OCT 10, 2018 12:00 PM PDTRNA sequencing unlocks the mysteries hidden in the transcriptome. Whether your goal is gene expression analysis, gene fusion analysis, SNP analysis or miRNA expression analysis, achieving hig...Speaker: Jonathan Shaffer, MBA, PhDPresented At:Cancer Research & Oncology 2018Sponsored By: QIAGENOCT 10, 2018 12:00 PM PDTC.E. CREDITS
OCT 10, 2018 12:00 PM PDTTo date the anatomic extent of tumor (TNM-classification) has been by far the most important factors to predict the prognosis of cancer patients. However, this classification provides limited...Speaker: Jerome Galon, PhDPresented At:Cancer Research & Oncology 2018OCT 10, 2018 09:00 AM PDTC.E. CREDITS
OCT 10, 2018 09:00 AM PDTIn prior work, we have pursued how tumor reactive monoclonal antibody (mAb), together with activators of innate immune cells, like NK cells, can augment antibody dependent cell-mediated cytot...Speaker: Paul Sondel, MD, PhDPresented At:Cancer Research & Oncology 2018OCT 10, 2018 09:00 AM PDTC.E. CREDITS
OCT 10, 2018 09:00 AM PDTHigh-throughput screening is widely useful in identifying genes and pathways that drive changes in cell behavior such as cell cycle regulation, metastasis, and drug resistance. Millipor...Speaker: Andrew Ravanelli, PhDPresented At:Cancer Research & Oncology 2018Sponsored By: MilliporeSigmaOCT 10, 2018 07:30 AM PDTC.E. CREDITS
OCT 10, 2018 07:30 AM PDTMolecular analyses of cancer biology have tended to segregate between a focus on nucleic acids – DNA, RNA and their modifications – and a focus on proteins and protein function. P...Speaker: Karin Rodland, PhDPresented At:Cancer Research & Oncology 2018OCT 10, 2018 06:00 AM PDTC.E. CREDITS
OCT 10, 2018 06:00 AM PDTUnderstanding the immune repertoire is an important aspect of immuno-oncology research, which can be used to gather insights into the function and overall status of the immune system. We have...Speaker: Samuel Rulli, PhDPresented At:Cancer Research & Oncology 2018Sponsored By: QIAGENOCT 03, 2018 09:00 AM PDTC.E. CREDITS
OCT 03, 2018 09:00 AM PDTDATE: October 3, 2018TIME: 9:00am PDT, 12:00pm EDT Infiltrating myeloid cells constitute a significant component of the suppressive tumor microenvironment (TME) a...Speaker: Christopher GrangeSponsored By: Cell Signaling TechnologyJUN 05, 2018 06:30 AM PDTC.E. CREDITS
JUN 05, 2018 06:30 AM PDTDATE: June 5, 2018TIME: 06:30AM PDT, 3:30PM CEST Viscous liquids are a challenge in every lab that deals with them. Depending on how viscous a liquid is, pipetting is either...Speaker: Hanae Henke, PhDSponsored By: EppendorfMAY 03, 2018 11:00 AM PDT
MAY 03, 2018 11:00 AM PDTDATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...Speaker: Sisi LiSponsored By: CovarisMAR 27, 2018 09:00 AM PDTC.E. CREDITS
MAR 27, 2018 09:00 AM PDTDATE: March 27, 2018TIME: 09:00am PST, 12:00pm EST...Speaker: Matthew Stokes, PhD , Lilian Phu , Don Kirkpatrick, PhDSponsored By: Cell Signaling TechnologyOCT 12, 2017 09:00 AM PDTC.E. CREDITS
OCT 12, 2017 09:00 AM PDTIn order to expand the treatment options of cancers driven by oncogenic RAS, new cell surface targets need to be identified and characterized. Here, we describe mass spectrometry based phenot...Speaker: Josip Blonder, MDPresented At:Cancer Research & Oncology 2017
MAY 16, 2019
04:00 PM CEST
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MAY 16, 2019
04:00 PM CEST
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DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
Speaker:
Rita Colwell, PhD
,
Christiane Honisch, PhD
Sponsored By: Eppendorf
MAY 23, 2019
09:00 AM PDT
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MAY 23, 2019
09:00 AM PDT
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DATE: May 23, 2019TIME: 9:00am PDT, 12:00pm EDT Although mesenchymal stem/stromal cells (MSCs) chondrogenic differentiation has been thoroughly investigated...
Speaker:
Melika Sarem, PhD
Sponsored By: Thermo Fisher Scientific/Gibco
JUN 05, 2019
05:00 PM CEST
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JUN 05, 2019
05:00 PM CEST
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DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
Speaker:
Jessica Wagener, PhD
Sponsored By: Eppendorf
APR 09, 2019
09:00 AM PDT
C.E. CREDITS
APR 09, 2019
09:00 AM PDT
DATE: April 9, 2019TIME: 9:00 AM PDTWe will provide a general introduction to the Single-Cell Western Technology (SCW) and then present data from 2 ongoing projects from the Col...
Speaker:
Noemi Kedei, MD
,
Kelly Gardner, Ph.D.
Sponsored By: ProteinSimple
DEC 20, 2018
09:00 AM CST
C.E. CREDITS
DEC 20, 2018
09:00 AM CST
DATE: December 20, 2018TIME: 09:00am CST, 10:00am JST Host cell protein (HCP) is a significant class of process-related impurity that needs to be removed to adequatel...
Speaker:
Fengqiang Wang, PhD
Sponsored By: GE Healthcare Life Sciences
DEC 18, 2018
07:00 AM PST
C.E. CREDITS
DEC 18, 2018
07:00 AM PST
DATE: December 18,2018TIME: 07:00am PST, 10:00am EST, 15:00pm GMT Host cell protein (HCP) is a significant class of process-related impurity that needs to be removed to...
Speaker:
Fengqiang Wang, PhD
Sponsored By: GE Healthcare Life Sciences
DEC 13, 2018
09:00 AM PST
C.E. CREDITS
DEC 13, 2018
09:00 AM PST
DATE: December 13 15,2018TIME: 09:00am PST, 12:00pm EST Breast cancers are classified into three main subtypes according to their receptor status: estrogen receptor-positive...
Speaker:
Hillary Stires, PhD
Sponsored By: Thermo Fisher Scientific/Gibco
OCT 11, 2018
01:30 PM PDT
C.E. CREDITS
OCT 11, 2018
01:30 PM PDT
As the most common female malignancy, breast cancer is the most likely reason that a woman will die of cancer around the world. Breast cancer mortality has dropped in the U.S. by 35% since 19...
Speaker:
Benjamin Anderson, MD
Presented At:Cancer Research & Oncology 2018
OCT 11, 2018
12:00 PM PDT
C.E. CREDITS
OCT 11, 2018
12:00 PM PDT
Lung cancer is the leading cause of cancer-related mortality worldwide. Large-scale sequencing studies have revealed the complex genomic landscape of NSCLC and genomic differences between lun...
Speaker:
Nicholas McGranahan, PhD
Presented At:Cancer Research & Oncology 2018
OCT 11, 2018
10:30 AM PDT
C.E. CREDITS
OCT 11, 2018
10:30 AM PDT
Two projects looking at novel approaches to targeting inflammatory breast cancer will be presented. Inflammatory breast cancer (IBC) is a unique, understudied, and most lethal subtype account...
Speaker:
Kevin Williams, PhD
Presented At:Cancer Research & Oncology 2018
OCT 11, 2018
09:00 AM PDT
C.E. CREDITS
OCT 11, 2018
09:00 AM PDT
The oncogenic transcription factor c-MYC (MYC) is deregulated, and often overexpressed, in more than 50% of cancers. MYC deregulation is associated with poor prognosis and aggressive disease,...
Speaker:
Jason De Melo, PhD
Presented At:Cancer Research & Oncology 2018
OCT 11, 2018
07:30 AM PDT
C.E. CREDITS
OCT 11, 2018
07:30 AM PDT
In the past two decades a small number of infrequently dividing cells have been proposed as the source of multi-drug resistance during cancer treatment. These cells identified by their expres...
Speaker:
Krastan Blagoev, PhD
Presented At:Cancer Research & Oncology 2018
OCT 11, 2018
06:00 AM PDT
C.E. CREDITS
OCT 11, 2018
06:00 AM PDT
PacBio Sequencing simultaneously provides long sequence reads, high consensus accuracy, minimal sequence bias, and methylation detection. I will highlight new advances and updates on applying...
Speaker:
Jonas Korlach, PhD
Presented At:Cancer Research & Oncology 2018
OCT 10, 2018
12:00 PM PDT
C.E. CREDITS
OCT 10, 2018
12:00 PM PDT
RNA sequencing unlocks the mysteries hidden in the transcriptome. Whether your goal is gene expression analysis, gene fusion analysis, SNP analysis or miRNA expression analysis, achieving hig...
Speaker:
Jonathan Shaffer, MBA, PhD
Presented At:Cancer Research & Oncology 2018
Sponsored By: QIAGEN
OCT 10, 2018
12:00 PM PDT
C.E. CREDITS
OCT 10, 2018
12:00 PM PDT
To date the anatomic extent of tumor (TNM-classification) has been by far the most important factors to predict the prognosis of cancer patients. However, this classification provides limited...
Speaker:
Jerome Galon, PhD
Presented At:Cancer Research & Oncology 2018
OCT 10, 2018
09:00 AM PDT
C.E. CREDITS
OCT 10, 2018
09:00 AM PDT
In prior work, we have pursued how tumor reactive monoclonal antibody (mAb), together with activators of innate immune cells, like NK cells, can augment antibody dependent cell-mediated cytot...
Speaker:
Paul Sondel, MD, PhD
Presented At:Cancer Research & Oncology 2018
OCT 10, 2018
09:00 AM PDT
C.E. CREDITS
OCT 10, 2018
09:00 AM PDT
High-throughput screening is widely useful in identifying genes and pathways that drive changes in cell behavior such as cell cycle regulation, metastasis, and drug resistance. Millipor...
Speaker:
Andrew Ravanelli, PhD
Presented At:Cancer Research & Oncology 2018
Sponsored By: MilliporeSigma
OCT 10, 2018
07:30 AM PDT
C.E. CREDITS
OCT 10, 2018
07:30 AM PDT
Molecular analyses of cancer biology have tended to segregate between a focus on nucleic acids – DNA, RNA and their modifications – and a focus on proteins and protein function. P...
Speaker:
Karin Rodland, PhD
Presented At:Cancer Research & Oncology 2018
OCT 10, 2018
06:00 AM PDT
C.E. CREDITS
OCT 10, 2018
06:00 AM PDT
Understanding the immune repertoire is an important aspect of immuno-oncology research, which can be used to gather insights into the function and overall status of the immune system. We have...
Speaker:
Samuel Rulli, PhD
Presented At:Cancer Research & Oncology 2018
Sponsored By: QIAGEN
OCT 03, 2018
09:00 AM PDT
C.E. CREDITS
OCT 03, 2018
09:00 AM PDT
DATE: October 3, 2018TIME: 9:00am PDT, 12:00pm EDT Infiltrating myeloid cells constitute a significant component of the suppressive tumor microenvironment (TME) a...
Speaker:
Christopher Grange
Sponsored By: Cell Signaling Technology
JUN 05, 2018
06:30 AM PDT
C.E. CREDITS
JUN 05, 2018
06:30 AM PDT
DATE: June 5, 2018TIME: 06:30AM PDT, 3:30PM CEST Viscous liquids are a challenge in every lab that deals with them. Depending on how viscous a liquid is, pipetting is either...
Speaker:
Hanae Henke, PhD
Sponsored By: Eppendorf
MAY 03, 2018
11:00 AM PDT
MAY 03, 2018
11:00 AM PDT
DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
Speaker:
Sisi Li
Sponsored By: Covaris
MAR 27, 2018
09:00 AM PDT
C.E. CREDITS
MAR 27, 2018
09:00 AM PDT
DATE: March 27, 2018TIME: 09:00am PST, 12:00pm EST...
Speaker:
Matthew Stokes, PhD
,
Lilian Phu
,
Don Kirkpatrick, PhD
Sponsored By: Cell Signaling Technology
OCT 12, 2017
09:00 AM PDT
C.E. CREDITS
OCT 12, 2017
09:00 AM PDT
In order to expand the treatment options of cancers driven by oncogenic RAS, new cell surface targets need to be identified and characterized. Here, we describe mass spectrometry based phenot...
Speaker:
Josip Blonder, MD
Presented At:Cancer Research & Oncology 2017