MENU

Analysis of Terpenes in Hemp by Headspace/Gas Chromatography/Mass Spectrometry

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Commercial Operations Manager for Think20 Laboratories
    Biography
      Adam Floyd currently serves as the Commercial Operations Manager for Think20 Laboratories. He has over ten years of professional experience in the cannabis industry with previous roles at Vermicrop Organics, General Hydroponics, The Hawthorne Gardening Company, and PerkinElmer. He holds a Bachelor of Science in Biochemistry and his specialties include mass spectrometry and gas chromatography.

    Abstract

    The objective of this study was to produce a fast, accurate, and precise method for analyzing terpenes in hemp using headspace/gas chromatography/mass spectrometry. Modern interest in terpenes and terpene analysis has grown significantly as the recreational and medicinal use of hemp has expanded across the globe. There is research that suggests terpenes provide a synergistic “entourage” effect, compounding the suggested medicinal benefits from CBD usage. Research into the relationship between the endocannabinoid system and terpenes has grown significantly.

    Terpenes are built from repeating five carbon units called isoprene. Their distinction as monoterpenes, sesquiterpenes, diterpenes, etc. is dependent on the number of isoprene units utilized in their biosynthesis. These compounds have traditionally been measured using liquid injection gas chromatography systems. Liquid injection presents a number of problems in a production environments such as dirty injection ports, persistent maintenance, and column contamination. The use of headspace eliminates these issues and provides robust sample introduction. The use of mass spectrometry allows for the separation and quantification of co-eluting peaks. This in turn allows for the rapid analysis of samples as chromatographic separation becomes less critical. Traditional use of FID (flame ionization detectors) demands complete chromatographic separation and extended run times (upwards of one hour per sample).

    Learning Objectives:

    1. Explain why terpenes are important in the hemp experience

    2. Explain the basics for terpene analysis is hemp

    3. Identify the importance of good sample preparation prior to terpene analysis


    Show Resources
    You May Also Like
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    OCT 08, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    OCT 08, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    APR 27, 2020 9:00 AM PDT
    C.E. CREDITS
    APR 27, 2020 9:00 AM PDT
    DATE: April 27, 2020 TIME: 9:00am PT, 12:00pm ET Recombinant adeno-associated virus (rAAV) is a small (25-nm) virus that is composed of a protein shell called a capsid and a single-stranded...
    JUN 09, 2020 10:00 AM PDT
    C.E. CREDITS
    JUN 09, 2020 10:00 AM PDT
    DATE: June 9, 2020 TIME: 10:00am PT, 1:00pm ET The presentation will first discuss sepsis as a disease and then explain the importance of performing diagnostic tests in the clinical labora...
    MAY 04, 2020 9:00 AM PDT
    C.E. CREDITS
    MAY 04, 2020 9:00 AM PDT
    DATE: May 4, 2020 TIME: 9:00am PT, 12:00pm ET...
    Loading Comments...
    Show Resources