Field Applications Scientist, Cell Therapy, Thermo Fisher Scientific
BIOGRAPHY
DEC 06, 2018 9:00 AM PST
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Cell and Gene Therapy Solutions: Enabling PSC Translation Workflows
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Abstract
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DATE: December 6, 2018
TIME: 9:00am PST, 12:00pm EST
Induced pluripotent stem cells (iPSC) intended for translational applications need to be of high quality; free of reprogramming transgenes, clear of adventitious agents, genetically stable, and functionally pluripotent. Generation of such cells requires consistent reprogramming, defined culture reagents and comprehensive characterization. CytoTune Sendai virus reprogramming enables consistent and robust reprogramming of diverse somatic cell types. Here we report the first off-the-shelf (CTS) Sendai virus reprogramming kit and a xeno-free workflow from fibroblast and blood cells designed for clinical and translational use. iPSCs were generated under these conditions from fibroblasts, CD34+ and T cells. To further streamline characterization, three molecular methods PluriTest, ScoreCard, and KaryoStat were used to confirm pluripotency, trilineage differentiation potential and genome integrity of the iPS cells.
Learning Objectives:
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The importance of consistent reprogramming, defined culture reagents and comprehensive characterization for generation of iPSC cells
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Details for the first off-the-shelf (CTS) Sendai virus reprogramming kit
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Review of three molecular methods PluriTest, ScoreCard, and KaryoStat and how they were used to confirm pluripotency
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DEC 06, 2018 9:00 AM PST
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Cell and Gene Therapy Solutions: Enabling PSC Translation Workflows
Specialty
Cell Biology
Cancer Research
Molecular Biology
Biotechnology
Genetics
Clinical Research
Cell Culture
Cancer Therapeutics
Biochemistry
Immunology
Stem Cell Technologies
Biology
Drug Discovery
Laboratory Testing
Clinical Diagnostics
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