DEC 06, 2018 09:00 AM PST

Cell and Gene Therapy Solutions: Enabling PSC Translation Workflows

Speakers
  • Field Applications Scientist, Cell Therapy, Thermo Fisher Scientific
    Biography
      Doctorate in Cell and Molecular Biology from Tulane University. Studied chronic variable stress and its effects on dendritic morphology and synaptic plasticity. From there, I did an NIH fellowship at UCSD looking at Alzheimer's disease and stress hormone signaling in human hippocampus and human NSCs (neural stem cells) derived from iPSCs. Spent the last 5 years working in cell therapy with regard to primary cells, 510k medical devices, and cell separation technologies. My favorite things to do in my free time are travel the worlds and see it's many museums, and go hiking or to the beach with my dog.

    Abstract:
    Register now for live event or to view the webinar on demand if you cannot make the live time.
     
    DATE:   December 6, 2018
    TIME:    9:00am PST, 12:00pm EST
     
    Induced pluripotent stem cells (iPSC) intended for translational applications need to be of high quality; free of reprogramming transgenes, clear of adventitious agents, genetically stable, and functionally pluripotent.  Generation of such cells requires consistent reprogramming, defined culture reagents and comprehensive characterization.  CytoTune Sendai virus reprogramming enables consistent and robust reprogramming of diverse somatic cell types. Here we report the first off-the-shelf (CTS) Sendai virus reprogramming kit and a xeno-free workflow from fibroblast and blood cells designed for clinical and translational use. iPSCs were generated under these conditions from fibroblasts, CD34+ and T cells.  To further streamline characterization, three molecular methods PluriTest, ScoreCard, and KaryoStat were used to confirm pluripotency, trilineage differentiation potential and genome integrity of the iPS cells.  
     
    Learning Objectives:
     
    • The importance of consistent reprogramming, defined culture reagents and comprehensive characterization for generation of iPSC cells
    • Details for the first off-the-shelf (CTS) Sendai virus reprogramming kit
    • Review of three molecular methods PluriTest, ScoreCard, and KaryoStat and how they were used to confirm pluripotency
     
    Watch our related webinars here:

    Show Resources
    You May Also Like
    MAY 16, 2019 04:00 PM CEST
    C.E. CREDITS
    MAY 16, 2019 04:00 PM CEST
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    SEP 05, 2019 04:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 04:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    NOV 18, 2019 08:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    NOV 18, 2019 08:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: November 18, 2019TIME: 8:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    JUN 05, 2019 05:00 PM CEST
    C.E. CREDITS
    JUN 05, 2019 05:00 PM CEST
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    AUG 13, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 13, 2019 09:00 AM PDT
    DATE: August 13, 2019TIME: 9:00am PT, 12:00pm ET, 5:00pm BST Molecular complexes are major constituents of cells, hence unraveling their mechanisms is key to fuller comprehension of c...
    Loading Comments...
    Show Resources