DATE: May 3, 2018
TIME: 11:00AM PDT, 2:00PM EDT
While stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in brain chemistry leading to mental illness remains poorly understood. Thus, my research has focused on the novel environmentally sensitive DNA methylation mark 5-hydryoxymethycytosine (5hmC) which is highly enriched in the brain, sensitive to stress, and associated with active neuronal transcription.
During my dissertation work, we have:
With this information at hand, using chromatin immunoprecipitation (ChIP) from a limited amount of brain tissue, we showed that stress resulted in a significant decrease in the binding of a transcription factor to one of the candidate genes with the highest altered 5hmC level due to stress. We further supported our ChIP-qPCR findings using electrophoretic shift assay (EMSA) and western blot. Together, the data from these molecular analyses suggest that 5hmC alters TF binding in response to stress.
In this webinar, the speaker will:
Cell Signaling / Transduction
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