JAN 31, 2019 6:00 AM PST

Overcoming Challenges in EuCODE Cell Culture Platform Development

Presented at: BioProcessing 2019
Speaker

Abstract

Ambrx’s mammalian expression platform (EuCODE™) enables non-native amino acids (nnAAs) through an expanded genetic code to both generate novel bio-therapeutics and to optimize the performance of antibody drug conjugate (ADC), therapeutic proteins, monoclonal antibodies (mAbs), and, bi- and multi-specific medicines. While the ability to control the defined Drug-to-Antibody Ratio (DAR) and payload site can provide an advantage to an ADC, the site-specific incorporation of the nnAAs into the antibody heavy chain introduces a unique challenge for antibody production.

To enable higher performance benchmarks in time and resources for process development with stringent product quality requirements, a proprietary cell culture platform is being developed and demonstrated fast-track development of high-quality, high-titer processes for producing recombinant proteins from CHO cells. 

We successfully generated a CHO-K1 cell line, stably expressing engineered amber suppressor tRNA and its cognate tRNA synthetase specific for nnAA para-acetyl phenylalanine (pAF), to achieve high production of monoclonal antibodies (mAbs) containing nnAAs. The stable cell lines were further evolved using CRISPR/Cas9 genome editing technology to sequentially knock out selected genes in glutamine synthesis, and, apoptosis pathways to improve selection efficiency and prevent loss of viable cell mass in production cultures, respectively.  Inhibition of apoptosis pathway leads to dramatic increase in viable cell mass and results in extended production time and increased productivity. 

In this presentation, we will discuss overcoming the challenges in cell culture platform development including cell line engineering, systematic DoE-based approaches on optimal chemically defined media and cell culture processes, and, strategies for scale up to clinical and commercial scales.

Learning Objectives: 

1. Learn what is cell culture platform and how to development your own cell culture platform
2. Learn the strategy to develop your own chemically-defined media


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
OCT 21, 2021 9:00 AM PDT
C.E. CREDITS
OCT 21, 2021 9:00 AM PDT
Date: October 21, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Industry guidelines for host cell protein impurity analysis emphasize the importance of process-specific and product-specific assessm...
JUN 03, 2021 12:00 PM CST
JUN 03, 2021 12:00 PM CST
DATE: June 3, 2021 TIME: 12:00pm SGT This webinar is a virtual event that focuses on utilizing the Gibco CTS Rotea System for Cell and Gene Therapy...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
JAN 31, 2019 6:00 AM PST

Overcoming Challenges in EuCODE Cell Culture Platform Development

Presented at: BioProcessing 2019

Specialty

Biochemistry

Biotechnology

Microbiology

Molecular Biology

Research

Cell

Immunity

Bioproduction

Disease

Bioprocess

Laboratory Testing

Neuroscience

Cell Culture

Health

Chemistry

Geography

North America50%

Asia33%

Europe17%

Registration Source

Website Visitors100%

Job Title

Student33%

Medical Doctor/Specialist17%

Medical Laboratory Technician17%

Scientist17%

Lab Management17%

Organization

Academic Institution50%

Clinical Laboratory17%

Biotech Company17%

Manufacturer - Other17%


Show Resources
Loading Comments...
Show Resources