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    RT-PCR

    RT-PCR: Reverse transcription polymerase chain reaction, a variant of polymerase chain reaction, is a technique commonly used in molecular biology to detect RNA expression. RT-PCR is often confused with real-time polymerase chain reaction by students and scientists alike, but they are separate and distinct techniques.

    Webinars

    • JUL 07, 2020 | 8:00 AM
      Development of an extraction-free, saliva-based workflow for SARS-CoV-2 detection with the Biomarkâ„¢ HD platform
      Development of an extraction-free, saliva-based workflow for SARS-CoV-2...
      The need for high-throughput laboratory testing to identify SARS-CoV-2 infected individuals has reached nearly unprecedented levels. This demand has also led to supply chain issues that have...
      Speaker: David King, PhD , Richard Head, MS
      Presented at: Fluidigm Infectious Disease and Oncology Virtual Summit
      1
      JUL 01, 2020 | 2:00 PM
      Operational strategies in molecular profiling of lung cancers
      Operational strategies in molecular profiling of lung cancers
      Lung cancer management has changed over the last few years with several actionable targets being recognised and mandated in the optimal management of these patients. The biomarker ordering p...
      Speaker: Sudha Murthy, MD
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      1
      JUL 01, 2020 | 12:45 PM
      Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent Genexus System
      Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent...
      NGS is still perceived as a complex, hard to implement and validate method that requires highly experienced people. Outsourcing next-generation sequencing (NGS) testing could be an option to...
      Speaker: Prof. Dr. Michael Hummel
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      1
      JUN 17, 2020 | 12:00 PM
      Panel discussion: Critical design considerations for COVID-19 rapid POC diagnostic test development
      Panel discussion: Critical design considerations for COVID-19...
      The spread of the SARS-COV-2 (COVID-19) pandemic is driving an urgent need for extensive testing, in order to quickly and accurately assess both who is infected, and who has been infected. I...
      Speaker: Klaus Hochleitner, PhD , Brendan O'Farrell, PhD , Darren Rowles , David Wilson , Lee Jenkins
      Presented at: Coronavirus Series
      Sponsored By: Cytiva
      2 20
      JUN 17, 2020 | 10:30 AM
      Sleep Better Without Testing Nightmares: Critical Considerations When Choosing an RT-PCR Assay for SARS-CoV-2 Testing
      Sleep Better Without Testing Nightmares: Critical Considerations...
      Choosing the right solution for your laboratory’s SARS-CoV-2 testing needs can be a difficult decision. With the dynamic demand on labs, making the wrong choice could be catastrophic....
      Speaker: Arvind Kothandaraman
      Presented at: Coronavirus Series
      Sponsored By: PerkinElmer
      1
      JUN 17, 2020 | 7:30 AM
      Effective RNA extraction for COVID-19 testing
      Effective RNA extraction for COVID-19 testing
      The spread of the SARS-COV-2 (COVID-19) pandemic is driving an urgent need for extensive testing, including patients and health workers as a priority. The most sensitive and reliable method...
      Speaker: Gabriel Fernandez de Pierola , Christopher Norey, PhD
      Presented at: Coronavirus Series
      Sponsored By: Cytiva
      1
      MAY 20, 2020 | 12:00 AM
      Highly sensitive detection of SARS-CoV-2 by Crystal Digital PCRâ„¢
      Highly sensitive detection of SARS-CoV-2 by Crystal Digital PCRâ„¢
      The current gold standard for COVID-19 diagnosis is based on the detection of SARS-CoV-2 nucleic acid using real-time quantitative PCR (RT-qPCR). Recent studies suggest limitations of RT-qPC...
      Speaker: Rémi Dangla
      Presented at: Laboratory Automation & Informatics Virtual Event Series 2020
      1
      MAY 19, 2020 | 8:00 AM
      Looking Beyond PCR: Isothermal Amplification
      Looking Beyond PCR: Isothermal Amplification
      DATE: May 19, 2020 TIME: 8:00am PT, 11:00am ET Limited sample material resulting in insufficient DNA input is a common hurdle for downstream analysis. This problem can be solved using a vari...
      Speaker: Agne Alminaite, PhD , Remigijus Skirgaila, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      71 5
      MAY 14, 2020 | 3:30 PM
      Genexus - A Major Step Forward in Diagnostic NGS
      Genexus - A Major Step Forward in Diagnostic NGS
      NGS is still perceived as a complex, hard to implement and validate method that requires highly experienced people. Outsourcing next-generation sequencing (NGS) testing could be an option to...
      Speaker: Prof. Dr. Michael Hummel
      Presented at: OncomineWorld 2022: A Virtual NGS Education Meeting
      10
      MAY 14, 2020 | 3:30 PM
      Genexus - A Major Step Forward in Diagnostic NGS
      Genexus - A Major Step Forward in Diagnostic NGS
      NGS is still perceived as a complex, hard to implement and validate method that requires highly experienced people. Outsourcing next-generation sequencing (NGS) testing could be an option to...
      Speaker: Prof. Dr. Michael Hummel
      Presented at: OncomineWorld Autumn: A virtual NGS education meeting
      1
      APR 03, 2020 | 9:00 AM
      BioGX SARS-Cov-2 Reagents for BD MAXâ„¢ System Webinar
      BioGX SARS-Cov-2 Reagents for BD MAXâ„¢ System Webinar
      DATE: April 3, 2020 TIME: 9:00am PT, 12:00pm ET The BioGX SARS-CoV-2 Reagents for BD MAX™ System is a real-time RT-PCR test intended for the qualitative detection of nucleic acid from...
      Speaker: Nikos Pavlidis , Dr. Patrick Murray, Ph.D. , Celine Roger-Dalbert , Diane Kawa, PhD
      Sponsored By: BD
      5
      MAR 03, 2020 | 9:00 AM
      The Importance of CPO Carriers Within the Healthcare System
      The Importance of CPO Carriers Within the Healthcare System
      DATE: March 3, 2020 TIME: 9:00am PT, 12:00pm ET Despite the advances of new drugs and the lowering of carbapenem breakpoints to enhance detection of CRE, there should be no complacency about...
      Speaker: Kenneth Thomson, PhD
      Presented at: BD MAX Webinar Library
      Sponsored By: BD
      5 1
      DEC 04, 2019 | 7:00 AM
      Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
      Improve your reproducibility - Liquid Handling is a crucial factor...
      DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
      Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
      Sponsored By: Eppendorf
      12
      NOV 18, 2019 | 7:00 AM
      Protect your cells with proper pipetting - How liquid handling influences your cell culture work
      Protect your cells with proper pipetting - How liquid handling...
      DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
      Speaker: Nadine Mellies, PhD , Katja Baesler, PhD
      Sponsored By: Eppendorf
      22 2
      NOV 14, 2019 | 10:30 AM
      Identifying the PIK3CA mutations that make a difference in breast cancer with the QIAGEN therascreen® PIK3CA RGQ PCR Kit
      Identifying the PIK3CA mutations that make a difference in breast...
      Breast cancer is the most frequently occurring form of cancer in women. While 5-year survival rates for localized disease approach 99%, cases of advanced breast cancer have a much poorer pro...
      Speaker: Benjamin Chaffey, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2019
      Sponsored By: QIAGEN
      1
      SEP 30, 2019 | 8:00 AM
      Discovery and Verification of eQTLs Associated with Lung Adenocarcinoma
      Discovery and Verification of eQTLs Associated with Lung Adenocarcinoma
      DATE: September 30, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Expression quantitative trait loci (eQTLs) are SNPs that have an influence on expression levels of genes, and the ident...
      Speaker: Tommaso Dragani , Harita Veereshlingam, PhD
      Sponsored By: Thermo Fisher Scientific - Applied Biosystems
      9
      SEP 05, 2019 | 7:00 AM
      The bread and butter method of genomics: How to improve your (q)PCR
      The bread and butter method of genomics: How to improve your (q)PCR
      DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
      Speaker: Tim Schommartz, PhD
      Sponsored By: Eppendorf
      17 1
      JUL 25, 2019 | 9:00 AM
      The diagnosis matters: elevating the standard of care for women impacted by vaginitis and vaginosis
      The diagnosis matters: elevating the standard of care for women...
      DATE: July 25, 2019TIME: 9:00am PT, 12:00pm ET Vaginitis / vaginosis (V/V) is defined as a spectrum of conditions that cause vaginal and sometimes vulvar symptoms, such as itch...
      Speaker: Karissa Culbreath, Ph.D., D(ABMM)
      Presented at: BD MAX Webinar Library
      Sponsored By: BD
      7
      JUN 05, 2019 | 5:00 PM
      Everyday culture practice - Improving reproducibility in cell culture
      Everyday culture practice - Improving reproducibility in cell...
      DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
      Speaker: Jessica Wagener, PhD
      Sponsored By: Eppendorf
      23 2
      MAY 16, 2019 | 4:00 PM
      Towards Standardized Workflows - A focus on microbiome insights
      Towards Standardized Workflows - A focus on microbiome insights
      DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
      Speaker: Rita Colwell, PhD , Christiane Honisch, PhD
      Sponsored By: Eppendorf
      10 2
      NOV 14, 2018 | 8:00 AM
      Webinar #1:  Copy number analysis in transplant research
      Webinar #1: Copy number analysis in transplant research
      DATE: November 14, 2018TIME: 08:00am PST, 11:00am EST International Genetics & Translational Research in Transplantation Network (iGeneTRAIN) has recently generated genome...
      Speaker: Brendan Keating, PhD
      Sponsored By: Thermo Fisher Scientific
      4
      AUG 07, 2018 | 8:00 AM
      Using Reference Standards to Validate your NGS Oncology Workflow
      Using Reference Standards to Validate your NGS Oncology Workflow
      DATE: August 7, 2018TIME: 08:00AM PDT, 11:00AM EDTWe are currently at an exciting precipice in clinical oncology management, where an increased rate of biomarker identification is drivi...
      Speaker: Hannah Child, PhD
      Sponsored By: Horizon Discovery
      10 1
      OCT 12, 2017 | 12:00 PM
      Detection of circulating tumour biomarkers using the FirePlex® Technology Platform
      Detection of circulating tumour biomarkers using the FirePlex®...
      The detection of circulating molecular biomarkers is useful for monitoring pathogenic processes and response to therapeutic intervention1,2. In cancer, genomic and proteomic biomarkers are us...
      Speaker: Daniel Pregibon, PhD
      Presented at: Abcam virtual exhibition: support and products to advance your research
      2
      OCT 12, 2017 | 12:00 PM
      Detection of circulating tumour biomarkers using the FirePlex® Technology Platform
      Detection of circulating tumour biomarkers using the FirePlex®...
      The detection of circulating molecular biomarkers is useful for monitoring pathogenic processes and response to therapeutic intervention1,2. In cancer, genomic and proteomic biomarkers are us...
      Speaker: Daniel Pregibon, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
      Sponsored By: Abcam
      1
    JUL 07, 2020 | 8:00 AM
    Development of an extraction-free, saliva-based workflow for SARS-CoV-2 detection with the Biomarkâ„¢ HD platform
    Development of an extraction-free, saliva-based workflow for SARS-CoV-2...
    The need for high-throughput laboratory testing to identify SARS-CoV-2 infected individuals has reached nearly unprecedented levels. This demand has also led to supply chain issues that have...
    Speaker: David King, PhD , Richard Head, MS
    Presented at: Fluidigm Infectious Disease and Oncology Virtual Summit
    1
    JUL 01, 2020 | 2:00 PM
    Operational strategies in molecular profiling of lung cancers
    Operational strategies in molecular profiling of lung cancers
    Lung cancer management has changed over the last few years with several actionable targets being recognised and mandated in the optimal management of these patients. The biomarker ordering p...
    Speaker: Sudha Murthy, MD
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    1
    JUL 01, 2020 | 12:45 PM
    Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent Genexus System
    Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent...
    NGS is still perceived as a complex, hard to implement and validate method that requires highly experienced people. Outsourcing next-generation sequencing (NGS) testing could be an option to...
    Speaker: Prof. Dr. Michael Hummel
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    1
    JUN 17, 2020 | 12:00 PM
    Panel discussion: Critical design considerations for COVID-19 rapid POC diagnostic test development
    Panel discussion: Critical design considerations for COVID-19...
    The spread of the SARS-COV-2 (COVID-19) pandemic is driving an urgent need for extensive testing, in order to quickly and accurately assess both who is infected, and who has been infected. I...
    Speaker: Klaus Hochleitner, PhD , Brendan O'Farrell, PhD , Darren Rowles , David Wilson , Lee Jenkins
    Presented at: Coronavirus Series
    Sponsored By: Cytiva
    2 20
    JUN 17, 2020 | 10:30 AM
    Sleep Better Without Testing Nightmares: Critical Considerations When Choosing an RT-PCR Assay for SARS-CoV-2 Testing
    Sleep Better Without Testing Nightmares: Critical Considerations...
    Choosing the right solution for your laboratory’s SARS-CoV-2 testing needs can be a difficult decision. With the dynamic demand on labs, making the wrong choice could be catastrophic....
    Speaker: Arvind Kothandaraman
    Presented at: Coronavirus Series
    Sponsored By: PerkinElmer
    1
     
     
  • JUN 17, 2020 | 7:30 AM
    Effective RNA extraction for COVID-19 testing
    Effective RNA extraction for COVID-19 testing
    The spread of the SARS-COV-2 (COVID-19) pandemic is driving an urgent need for extensive testing, including patients and health workers as a priority. The most sensitive and reliable method...
    Speaker: Gabriel Fernandez de Pierola , Christopher Norey, PhD
    Presented at: Coronavirus Series
    Sponsored By: Cytiva
    1
    MAY 20, 2020 | 12:00 AM
    Highly sensitive detection of SARS-CoV-2 by Crystal Digital PCRâ„¢
    Highly sensitive detection of SARS-CoV-2 by Crystal Digital PCRâ„¢
    The current gold standard for COVID-19 diagnosis is based on the detection of SARS-CoV-2 nucleic acid using real-time quantitative PCR (RT-qPCR). Recent studies suggest limitations of RT-qPC...
    Speaker: Rémi Dangla
    Presented at: Laboratory Automation & Informatics Virtual Event Series 2020
    1
    MAY 19, 2020 | 8:00 AM
    Looking Beyond PCR: Isothermal Amplification
    Looking Beyond PCR: Isothermal Amplification
    DATE: May 19, 2020 TIME: 8:00am PT, 11:00am ET Limited sample material resulting in insufficient DNA input is a common hurdle for downstream analysis. This problem can be solved using a vari...
    Speaker: Agne Alminaite, PhD , Remigijus Skirgaila, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    71 5
    MAY 14, 2020 | 3:30 PM
    Genexus - A Major Step Forward in Diagnostic NGS
    Genexus - A Major Step Forward in Diagnostic NGS
    NGS is still perceived as a complex, hard to implement and validate method that requires highly experienced people. Outsourcing next-generation sequencing (NGS) testing could be an option to...
    Speaker: Prof. Dr. Michael Hummel
    Presented at: OncomineWorld 2022: A Virtual NGS Education Meeting
    10
    MAY 14, 2020 | 3:30 PM
    Genexus - A Major Step Forward in Diagnostic NGS
    Genexus - A Major Step Forward in Diagnostic NGS
    NGS is still perceived as a complex, hard to implement and validate method that requires highly experienced people. Outsourcing next-generation sequencing (NGS) testing could be an option to...
    Speaker: Prof. Dr. Michael Hummel
    Presented at: OncomineWorld Autumn: A virtual NGS education meeting
    1
     
     
  • APR 03, 2020 | 9:00 AM
    BioGX SARS-Cov-2 Reagents for BD MAXâ„¢ System Webinar
    BioGX SARS-Cov-2 Reagents for BD MAXâ„¢ System Webinar
    DATE: April 3, 2020 TIME: 9:00am PT, 12:00pm ET The BioGX SARS-CoV-2 Reagents for BD MAX™ System is a real-time RT-PCR test intended for the qualitative detection of nucleic acid from...
    Speaker: Nikos Pavlidis , Dr. Patrick Murray, Ph.D. , Celine Roger-Dalbert , Diane Kawa, PhD
    Sponsored By: BD
    5
    MAR 03, 2020 | 9:00 AM
    The Importance of CPO Carriers Within the Healthcare System
    The Importance of CPO Carriers Within the Healthcare System
    DATE: March 3, 2020 TIME: 9:00am PT, 12:00pm ET Despite the advances of new drugs and the lowering of carbapenem breakpoints to enhance detection of CRE, there should be no complacency about...
    Speaker: Kenneth Thomson, PhD
    Presented at: BD MAX Webinar Library
    Sponsored By: BD
    5 1
    DEC 04, 2019 | 7:00 AM
    Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
    Improve your reproducibility - Liquid Handling is a crucial factor...
    DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
    Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
    Sponsored By: Eppendorf
    12
    NOV 18, 2019 | 7:00 AM
    Protect your cells with proper pipetting - How liquid handling influences your cell culture work
    Protect your cells with proper pipetting - How liquid handling...
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    Speaker: Nadine Mellies, PhD , Katja Baesler, PhD
    Sponsored By: Eppendorf
    22 2
    NOV 14, 2019 | 10:30 AM
    Identifying the PIK3CA mutations that make a difference in breast cancer with the QIAGEN therascreen® PIK3CA RGQ PCR Kit
    Identifying the PIK3CA mutations that make a difference in breast...
    Breast cancer is the most frequently occurring form of cancer in women. While 5-year survival rates for localized disease approach 99%, cases of advanced breast cancer have a much poorer pro...
    Speaker: Benjamin Chaffey, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2019
    Sponsored By: QIAGEN
    1
     
     
  • SEP 30, 2019 | 8:00 AM
    Discovery and Verification of eQTLs Associated with Lung Adenocarcinoma
    Discovery and Verification of eQTLs Associated with Lung Adenocarcinoma
    DATE: September 30, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Expression quantitative trait loci (eQTLs) are SNPs that have an influence on expression levels of genes, and the ident...
    Speaker: Tommaso Dragani , Harita Veereshlingam, PhD
    Sponsored By: Thermo Fisher Scientific - Applied Biosystems
    9
    SEP 05, 2019 | 7:00 AM
    The bread and butter method of genomics: How to improve your (q)PCR
    The bread and butter method of genomics: How to improve your (q)PCR
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    Speaker: Tim Schommartz, PhD
    Sponsored By: Eppendorf
    17 1
    JUL 25, 2019 | 9:00 AM
    The diagnosis matters: elevating the standard of care for women impacted by vaginitis and vaginosis
    The diagnosis matters: elevating the standard of care for women...
    DATE: July 25, 2019TIME: 9:00am PT, 12:00pm ET Vaginitis / vaginosis (V/V) is defined as a spectrum of conditions that cause vaginal and sometimes vulvar symptoms, such as itch...
    Speaker: Karissa Culbreath, Ph.D., D(ABMM)
    Presented at: BD MAX Webinar Library
    Sponsored By: BD
    7
    JUN 05, 2019 | 5:00 PM
    Everyday culture practice - Improving reproducibility in cell culture
    Everyday culture practice - Improving reproducibility in cell...
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    Speaker: Jessica Wagener, PhD
    Sponsored By: Eppendorf
    23 2
    MAY 16, 2019 | 4:00 PM
    Towards Standardized Workflows - A focus on microbiome insights
    Towards Standardized Workflows - A focus on microbiome insights
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    Speaker: Rita Colwell, PhD , Christiane Honisch, PhD
    Sponsored By: Eppendorf
    10 2
     
     
  • NOV 14, 2018 | 8:00 AM
    Webinar #1:  Copy number analysis in transplant research
    Webinar #1: Copy number analysis in transplant research
    DATE: November 14, 2018TIME: 08:00am PST, 11:00am EST International Genetics & Translational Research in Transplantation Network (iGeneTRAIN) has recently generated genome...
    Speaker: Brendan Keating, PhD
    Sponsored By: Thermo Fisher Scientific
    4
    AUG 07, 2018 | 8:00 AM
    Using Reference Standards to Validate your NGS Oncology Workflow
    Using Reference Standards to Validate your NGS Oncology Workflow
    DATE: August 7, 2018TIME: 08:00AM PDT, 11:00AM EDTWe are currently at an exciting precipice in clinical oncology management, where an increased rate of biomarker identification is drivi...
    Speaker: Hannah Child, PhD
    Sponsored By: Horizon Discovery
    10 1
    OCT 12, 2017 | 12:00 PM
    Detection of circulating tumour biomarkers using the FirePlex® Technology Platform
    Detection of circulating tumour biomarkers using the FirePlex®...
    The detection of circulating molecular biomarkers is useful for monitoring pathogenic processes and response to therapeutic intervention1,2. In cancer, genomic and proteomic biomarkers are us...
    Speaker: Daniel Pregibon, PhD
    Presented at: Abcam virtual exhibition: support and products to advance your research
    2
    OCT 12, 2017 | 12:00 PM
    Detection of circulating tumour biomarkers using the FirePlex® Technology Platform
    Detection of circulating tumour biomarkers using the FirePlex®...
    The detection of circulating molecular biomarkers is useful for monitoring pathogenic processes and response to therapeutic intervention1,2. In cancer, genomic and proteomic biomarkers are us...
    Speaker: Daniel Pregibon, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
    Sponsored By: Abcam
    1
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