Understanding the immune repertoire is an important aspect of immuno-oncology research, which can be used to gather insights into the function and overall status of the immune system. We have developed a simple and straightforward workflow with an RNA-seq panel, which uses Unique Molecular Indices (UMIs), and a data analysis package to characterize the alpha, beta, gamma and delta chains of the human or mouse T-cell receptors, including the CDR1, CDR2 and CDR3 regions. In this webinar, we will discuss both performance and application data for T-cell receptor sequencing and how a complete workflow including UMIs and UMI-enabled software helps increase the overall confidence in the final data analysis.
1. Understand the biology of T-cells & T-cell receptors
2. Identify different methods of T-cell clonotyping
3. Learn how unique molecular indexing is being used in NGS applications