High-throughput screening is widely useful in identifying genes and pathways that drive changes in cell behavior such as cell cycle regulation, metastasis, and drug resistance. MilliporeSigma develops and distributes a comprehensive portfolio of functional genomics tools for efficient, high quality, genome-scale analysis of cellular processes. In this webinar, we will demonstrate the utility of three genome-wide approaches to unbiased identification of relevant gene pathways for cancer discovery: shRNA knock-down, CRISPR knock-out, and CRISPR activator libraries.
1. Learn how to select reagents for whole-genome screens
2. Learn about complementarity between screening technologies
3. Learn example methods for candidate validation