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    Protocol

    A protocol is a preset description of methods that can be followed to perform a scientific experiment. Clear, concise protocols provide specific steps and a list of materials needed to complete the experiment. Protocols ensure that data can be reproduced by other scientists and researchers.

    Webinars

    • SEP 17, 2020 | 4:30 PM
      Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2
      Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2
      The COVID-19 pandemic has resulted in demand for population scale pathogen screening. The most sensitive and reliable method for detecting SARS-CoV-2 remains reverse transcription PCR (RT-PC...
      Speaker: Brian McNally, PhD
      Presented at: Coronavirus Series
      3
      AUG 06, 2020 | 12:00 AM
      Copper Hydride Catalysis for the Synthesis of Axially Chiral Allenes
      Copper Hydride Catalysis for the Synthesis of Axially Chiral Allenes
      Allenes, compounds containing two double bonds across three contiguous carbons, are versatile synthetic intermediates due to their substituent loading capacity and ability to generate one or...
      Speaker: Liela Romero, PhD
      Presented at: 2020 Beckman Symposium
      1
      AUG 04, 2020 | 8:00 AM
      Flexible, clinically adaptable, non-viral approaches to CAR TCR methodologies
      Flexible, clinically adaptable, non-viral approaches to CAR TCR...
      DATE: June 23, 2020 TIME: 6:00 am PDT, 9:00 am EDT In this webinar for scientists and researchers, Rama Shivakumar, a senior scientist at MaxCyte Inc., will highlight powerful case studies t...
      Speaker: Rama S Shivakumar
      Sponsored By: MaxCyte®
      7
      JUL 30, 2020 | 10:00 AM
      CliniMACS Prodigy® Adherent Cell Culture System: Pluripotent stem cell expansion & differentiation in an automated, closed system
      CliniMACS Prodigy® Adherent Cell Culture System: Pluripotent stem...
      DATE: July 30, 2020 TIME: 10:00am PT Regenerative medicine has become a key focus worldwide and the number of stem cell, especially pluripotent stem cell (PSC)-based clinical trials are rapi...
      Speaker: Dr. Chao Sheng
      Sponsored By: Miltenyi Biotec
      19 3
      JUL 01, 2020 | 8:00 AM
      Applied Biosystemsâ„¢ TaqPathâ„¢ COVID-19 Combo Kit*: A highly sensitive, specific, and robust real-time PCR test
      Applied Biosystemsâ„¢ TaqPathâ„¢ COVID-19 Combo Kit*: A highly sensitive,...
      DATE: July 1, 2020 TIME: 8:00 am PDT, 11:00 am EDT In late 2019, researchers identified the SARS-CoV-2 pathogen as the virus responsible for COVID-19. The virus spread rapidly throughout the...
      Speaker: Lauren Mikulski , Vijay Singh, PhD , Andrew Brooks, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      25 2
      JUN 25, 2020 | 7:00 AM
      Fully Automated Targeted SNP Genotyping for Plant and Animal Applications
      Fully Automated Targeted SNP Genotyping for Plant and Animal Applications
      DATE: June 25, 2020 TIME: 7:00am PT Genotyping assays are essential in a variety of plant and animal applications including Marker Assisted Selection (MAS) and Quantitative Trait Locus (QTL)...
      Speaker: Jürgen Zimmermann , Bjoern Textor
      Sponsored By: Beckman Coulter Life Sciences
      16
      JUN 24, 2020 | 8:00 AM
      CRISPR-Cas assisted genetics in intestinal organoids
      CRISPR-Cas assisted genetics in intestinal organoids
      DATE: June 24, 2020 TIME: 8:00am PT The identification of LGR5 + intestinal stem cells helped us to understand various aspects of adult stem cells and led to the establishment of primary 3D...
      Speaker: Bon-Kyoung Koo, PhD
      Sponsored By: GenScript
      33 5
      JUN 17, 2020 | 12:00 PM
      Luminex®: Flexible Solutions for SARS-CoV-2 Detection
      Luminex®: Flexible Solutions for SARS-CoV-2 Detection
      Join us and hear from two Luminex® experts on our flexible solutions for molecular diagnostics. Dr. Sherry Dunbar and Dr. Aaron Benfield will describe our NxTAG® bead-based nucleic a...
      Speaker: Aaron P. Benfield, PhD , Sherry A. Dunbar, PhD, MBA
      Presented at: Coronavirus Series
      Sponsored By: Luminex - A DiaSorin Company
      1
      JUN 17, 2020 | 7:30 AM
      Effective RNA extraction for COVID-19 testing
      Effective RNA extraction for COVID-19 testing
      The spread of the SARS-COV-2 (COVID-19) pandemic is driving an urgent need for extensive testing, including patients and health workers as a priority. The most sensitive and reliable method...
      Speaker: Gabriel Fernandez de Pierola , Christopher Norey, PhD
      Presented at: Coronavirus Series
      Sponsored By: Cytiva
      1
      JUN 12, 2020 | 8:00 AM
      Genome imaging of head and neck solid tumors: oropharyngeal, tongue, and thyroid cancers
      Genome imaging of head and neck solid tumors: oropharyngeal, tongue,...
      DATE: June 12, 2020 TIME: 08:00 am PDT, 11:00 am EDT Whole genome imaging using the Saphyr instrument from Bionano detects structural variants (SVs), such as insertions, deletions,...
      Speaker: Brandon LaBarge
      Sponsored By: Bionano
      21
      JUN 03, 2020 | 4:00 PM
      Microfluidic precision autostainer for fast multiplexed TSA-based immunofluorescence
      Microfluidic precision autostainer for fast multiplexed TSA-based...
      Multiplex immunofluorescence IHC is a powerful technique to address the increasing research needs in immuno-oncology to better understand the tumor microenvironment. In this webinar, we will...
      Speaker: Alexandre Kehren, M.Sc.
      Presented at: Immuno-Oncology Virtual Event Series 2020
      Sponsored By: Lunaphore
      2
      JUN 03, 2020 | 12:00 PM
      How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
      How multiplexed imaging reveals key insights into the tumor microenvironment...
      Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understanding of cells and their surroundings by providin...
      Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
      Presented at: Immuno-Oncology Virtual Event Series 2020
      Sponsored By: Cytiva
      0
      JUN 03, 2020 | 12:00 PM
      Single cell multiomic analysis of T-cell exhaustion
      Single cell multiomic analysis of T-cell exhaustion
      A key step in the clinical production of CAR T cells is the expansion of engineered T cells. To generate enough cells for viable adoptive cell therapy, cells must be robustly stimulated, whi...
      Speaker: Pratip K. Chattopadhyay
      Presented at: Immuno-Oncology Virtual Event Series 2020
      Sponsored By: BD
      2
      JUN 03, 2020 | 8:00 AM
      Keynote Presentation: It is Time for TIME (Tumor Immune Microenvironment) in Experimental and Diagnostic Pathology
      Keynote Presentation: It is Time for TIME (Tumor Immune Microenvironment)...
      Cancer initiation and virulence depends on mechanisms to evade host immunosurveillance and suppression of early invasion and growth. Distinct phenotypes of the immune infiltrates in and arou...
      Speaker: Alexander David Borowsky, MD
      Presented at: Immuno-Oncology Virtual Event Series 2020
      Sponsored By: Leica Biosystems
      1
      JUN 03, 2020 | 12:00 AM
      The Quagmire of Quantitative Confocal Microscopy
      The Quagmire of Quantitative Confocal Microscopy
      Confocal microscopes (and related instruments such as Two-Photon) are often used for the most rigorous quantitative fluorescence microscopy measurements. Most confocal users are aware of the...
      Speaker: James Jonkman
      Presented at: Cell & Developmental Biology Virtual Meeting
      3
      MAY 28, 2020 | 10:00 AM
      In situ immune profiling of heart transplant biopsies improves diagnostic accuracy and rejection risk stratification
      In situ immune profiling of heart transplant biopsies improves...
      DATE: May 28, 2020 TIME: 10:00am PT, 1:00pm ET Cardiac allograft rejection is a serious concern in transplant medicine, conferring an increased risk of acute graft failure and adverse patient...
      Speaker: Eliot Peyster, MD, MSc
      Sponsored By: Akoya Biosciences
      11
      MAY 27, 2020 | 8:00 AM
      How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
      How multiplexed imaging reveals key insights into the tumor microenvironment...
      DATE: May 27, 2020 TIME: 8:00am PT, 11:00am ET Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understand...
      Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
      Sponsored By: Cytiva
      18 2
      MAY 19, 2020 | 1:45 PM
      Cancer Transcriptome Atlas with NGS Readout - The essential tool for spatial genomics in oncology
      Cancer Transcriptome Atlas with NGS Readout - The essential tool...
      While many technologies focus on either exploring the spatial context of a handful of targets, or expression patterns of hundreds or thousands of targets in bulk samples or dissociated singl...
      Speaker: Kit Fuhrman, PhD
      Presented at: Advancing Science - A Virtual Oncology Conference
      1
      MAY 19, 2020 | 8:00 AM
      Looking Beyond PCR: Isothermal Amplification
      Looking Beyond PCR: Isothermal Amplification
      DATE: May 19, 2020 TIME: 8:00am PT, 11:00am ET Limited sample material resulting in insufficient DNA input is a common hurdle for downstream analysis. This problem can be solved using a vari...
      Speaker: Agne Alminaite, PhD , Remigijus Skirgaila, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      71 5
      MAY 14, 2020 | 9:00 AM
      PANEL: Experimental design and the reproducibility crisis: pitfalls, power, and practical best practice
      PANEL: Experimental design and the reproducibility crisis: pitfalls,...
      The justification of sample size is one of the hardest sections of a proposal an IACUC (ethical review board) has to assess, yet is arguably one of the most important (1). This is a key oppo...
      Speaker: Joseph Garner, PhD , Brianna Gaskill, PhD
      Presented at: Laboratory Animal Sciences Virtual Event Series 2020
      1
      MAY 14, 2020 | 12:00 AM
      Evaluation of Analgesia Efficacy in Postoperative Mice Using Novel Cage-Side Measures
      Evaluation of Analgesia Efficacy in Postoperative Mice Using Novel...
      The ability to rapidly and accurately identify pain in mice is critical for providing them optimal care and welfare. To meet this need, we developed and validated the Grooming Transfer Test...
      Speaker: Jennifer Lofgren, DVM, MS, DACLAM
      Presented at: Laboratory Animal Sciences Virtual Event Series 2020
      0
      MAY 12, 2020 | 9:00 AM
      Analytical Quality Control for the Pharmaceutical Industry
      Analytical Quality Control for the Pharmaceutical Industry
      NEW DATE: Date: May 12, 2020 Time: 9:00am PDT, 12:00pm EDT...
      Speaker: Joy McElroy
      Sponsored By: Sartorius
      29 9
      MAY 07, 2020 | 7:00 AM
      A complete workflow for analysis of cfDNA: from plasma to variants
      A complete workflow for analysis of cfDNA: from plasma to variants
      DATE: May 7, 2020 TIME: 7:00am PT As cost of sequencing continues to drop, the throughput and complexity of NGS assays have risen precipitously. At the same time, the types of samples being...
      Speaker: Shilpa Parakh , Nicole Roseman
      Sponsored By: Beckman Coulter Life Sciences, Integrated DNA Technologies (IDT)
      13 10
      APR 30, 2020 | 7:00 AM
      Nucleic Acid Purification - Understand the details, tackle the routines!
      Nucleic Acid Purification - Understand the details, tackle the...
      DATE: April 30, 2020 TIME: 7am PT, 10am ET, 4pm CEST Aged more than one century, nucleic acid purification is anything but ordinary. Nucleic Acid Purification, also termed Nucleic Acid Extra...
      Speaker: Andreas von Bohl, PhD , Milan Dieris, PhD , Tim Schommartz, PhD
      Sponsored By: Eppendorf, Macherey-Nagel
      45 13
    SEP 17, 2020 | 4:30 PM
    Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2
    Ultra-fast one-step RT-PCR protocol for the detection of SARS-CoV-2
    The COVID-19 pandemic has resulted in demand for population scale pathogen screening. The most sensitive and reliable method for detecting SARS-CoV-2 remains reverse transcription PCR (RT-PC...
    Speaker: Brian McNally, PhD
    Presented at: Coronavirus Series
    3
    AUG 06, 2020 | 12:00 AM
    Copper Hydride Catalysis for the Synthesis of Axially Chiral Allenes
    Copper Hydride Catalysis for the Synthesis of Axially Chiral Allenes
    Allenes, compounds containing two double bonds across three contiguous carbons, are versatile synthetic intermediates due to their substituent loading capacity and ability to generate one or...
    Speaker: Liela Romero, PhD
    Presented at: 2020 Beckman Symposium
    1
    AUG 04, 2020 | 8:00 AM
    Flexible, clinically adaptable, non-viral approaches to CAR TCR methodologies
    Flexible, clinically adaptable, non-viral approaches to CAR TCR...
    DATE: June 23, 2020 TIME: 6:00 am PDT, 9:00 am EDT In this webinar for scientists and researchers, Rama Shivakumar, a senior scientist at MaxCyte Inc., will highlight powerful case studies t...
    Speaker: Rama S Shivakumar
    Sponsored By: MaxCyte®
    7
    JUL 30, 2020 | 10:00 AM
    CliniMACS Prodigy® Adherent Cell Culture System: Pluripotent stem cell expansion & differentiation in an automated, closed system
    CliniMACS Prodigy® Adherent Cell Culture System: Pluripotent stem...
    DATE: July 30, 2020 TIME: 10:00am PT Regenerative medicine has become a key focus worldwide and the number of stem cell, especially pluripotent stem cell (PSC)-based clinical trials are rapi...
    Speaker: Dr. Chao Sheng
    Sponsored By: Miltenyi Biotec
    19 3
    JUL 01, 2020 | 8:00 AM
    Applied Biosystemsâ„¢ TaqPathâ„¢ COVID-19 Combo Kit*: A highly sensitive, specific, and robust real-time PCR test
    Applied Biosystemsâ„¢ TaqPathâ„¢ COVID-19 Combo Kit*: A highly sensitive,...
    DATE: July 1, 2020 TIME: 8:00 am PDT, 11:00 am EDT In late 2019, researchers identified the SARS-CoV-2 pathogen as the virus responsible for COVID-19. The virus spread rapidly throughout the...
    Speaker: Lauren Mikulski , Vijay Singh, PhD , Andrew Brooks, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    25 2
     
     
  • JUN 25, 2020 | 7:00 AM
    Fully Automated Targeted SNP Genotyping for Plant and Animal Applications
    Fully Automated Targeted SNP Genotyping for Plant and Animal Applications
    DATE: June 25, 2020 TIME: 7:00am PT Genotyping assays are essential in a variety of plant and animal applications including Marker Assisted Selection (MAS) and Quantitative Trait Locus (QTL)...
    Speaker: Jürgen Zimmermann , Bjoern Textor
    Sponsored By: Beckman Coulter Life Sciences
    16
    JUN 24, 2020 | 8:00 AM
    CRISPR-Cas assisted genetics in intestinal organoids
    CRISPR-Cas assisted genetics in intestinal organoids
    DATE: June 24, 2020 TIME: 8:00am PT The identification of LGR5 + intestinal stem cells helped us to understand various aspects of adult stem cells and led to the establishment of primary 3D...
    Speaker: Bon-Kyoung Koo, PhD
    Sponsored By: GenScript
    33 5
    JUN 17, 2020 | 12:00 PM
    Luminex®: Flexible Solutions for SARS-CoV-2 Detection
    Luminex®: Flexible Solutions for SARS-CoV-2 Detection
    Join us and hear from two Luminex® experts on our flexible solutions for molecular diagnostics. Dr. Sherry Dunbar and Dr. Aaron Benfield will describe our NxTAG® bead-based nucleic a...
    Speaker: Aaron P. Benfield, PhD , Sherry A. Dunbar, PhD, MBA
    Presented at: Coronavirus Series
    Sponsored By: Luminex - A DiaSorin Company
    1
    JUN 17, 2020 | 7:30 AM
    Effective RNA extraction for COVID-19 testing
    Effective RNA extraction for COVID-19 testing
    The spread of the SARS-COV-2 (COVID-19) pandemic is driving an urgent need for extensive testing, including patients and health workers as a priority. The most sensitive and reliable method...
    Speaker: Gabriel Fernandez de Pierola , Christopher Norey, PhD
    Presented at: Coronavirus Series
    Sponsored By: Cytiva
    1
    JUN 12, 2020 | 8:00 AM
    Genome imaging of head and neck solid tumors: oropharyngeal, tongue, and thyroid cancers
    Genome imaging of head and neck solid tumors: oropharyngeal, tongue,...
    DATE: June 12, 2020 TIME: 08:00 am PDT, 11:00 am EDT Whole genome imaging using the Saphyr instrument from Bionano detects structural variants (SVs), such as insertions, deletions,...
    Speaker: Brandon LaBarge
    Sponsored By: Bionano
    21
     
     
  • JUN 03, 2020 | 4:00 PM
    Microfluidic precision autostainer for fast multiplexed TSA-based immunofluorescence
    Microfluidic precision autostainer for fast multiplexed TSA-based...
    Multiplex immunofluorescence IHC is a powerful technique to address the increasing research needs in immuno-oncology to better understand the tumor microenvironment. In this webinar, we will...
    Speaker: Alexandre Kehren, M.Sc.
    Presented at: Immuno-Oncology Virtual Event Series 2020
    Sponsored By: Lunaphore
    2
    JUN 03, 2020 | 12:00 PM
    How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
    How multiplexed imaging reveals key insights into the tumor microenvironment...
    Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understanding of cells and their surroundings by providin...
    Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
    Presented at: Immuno-Oncology Virtual Event Series 2020
    Sponsored By: Cytiva
    0
    JUN 03, 2020 | 12:00 PM
    Single cell multiomic analysis of T-cell exhaustion
    Single cell multiomic analysis of T-cell exhaustion
    A key step in the clinical production of CAR T cells is the expansion of engineered T cells. To generate enough cells for viable adoptive cell therapy, cells must be robustly stimulated, whi...
    Speaker: Pratip K. Chattopadhyay
    Presented at: Immuno-Oncology Virtual Event Series 2020
    Sponsored By: BD
    2
    JUN 03, 2020 | 8:00 AM
    Keynote Presentation: It is Time for TIME (Tumor Immune Microenvironment) in Experimental and Diagnostic Pathology
    Keynote Presentation: It is Time for TIME (Tumor Immune Microenvironment)...
    Cancer initiation and virulence depends on mechanisms to evade host immunosurveillance and suppression of early invasion and growth. Distinct phenotypes of the immune infiltrates in and arou...
    Speaker: Alexander David Borowsky, MD
    Presented at: Immuno-Oncology Virtual Event Series 2020
    Sponsored By: Leica Biosystems
    1
    JUN 03, 2020 | 12:00 AM
    The Quagmire of Quantitative Confocal Microscopy
    The Quagmire of Quantitative Confocal Microscopy
    Confocal microscopes (and related instruments such as Two-Photon) are often used for the most rigorous quantitative fluorescence microscopy measurements. Most confocal users are aware of the...
    Speaker: James Jonkman
    Presented at: Cell & Developmental Biology Virtual Meeting
    3
     
     
  • MAY 28, 2020 | 10:00 AM
    In situ immune profiling of heart transplant biopsies improves diagnostic accuracy and rejection risk stratification
    In situ immune profiling of heart transplant biopsies improves...
    DATE: May 28, 2020 TIME: 10:00am PT, 1:00pm ET Cardiac allograft rejection is a serious concern in transplant medicine, conferring an increased risk of acute graft failure and adverse patient...
    Speaker: Eliot Peyster, MD, MSc
    Sponsored By: Akoya Biosciences
    11
    MAY 27, 2020 | 8:00 AM
    How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
    How multiplexed imaging reveals key insights into the tumor microenvironment...
    DATE: May 27, 2020 TIME: 8:00am PT, 11:00am ET Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understand...
    Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
    Sponsored By: Cytiva
    18 2
    MAY 19, 2020 | 1:45 PM
    Cancer Transcriptome Atlas with NGS Readout - The essential tool for spatial genomics in oncology
    Cancer Transcriptome Atlas with NGS Readout - The essential tool...
    While many technologies focus on either exploring the spatial context of a handful of targets, or expression patterns of hundreds or thousands of targets in bulk samples or dissociated singl...
    Speaker: Kit Fuhrman, PhD
    Presented at: Advancing Science - A Virtual Oncology Conference
    1
    MAY 19, 2020 | 8:00 AM
    Looking Beyond PCR: Isothermal Amplification
    Looking Beyond PCR: Isothermal Amplification
    DATE: May 19, 2020 TIME: 8:00am PT, 11:00am ET Limited sample material resulting in insufficient DNA input is a common hurdle for downstream analysis. This problem can be solved using a vari...
    Speaker: Agne Alminaite, PhD , Remigijus Skirgaila, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    71 5
    MAY 14, 2020 | 9:00 AM
    PANEL: Experimental design and the reproducibility crisis: pitfalls, power, and practical best practice
    PANEL: Experimental design and the reproducibility crisis: pitfalls,...
    The justification of sample size is one of the hardest sections of a proposal an IACUC (ethical review board) has to assess, yet is arguably one of the most important (1). This is a key oppo...
    Speaker: Joseph Garner, PhD , Brianna Gaskill, PhD
    Presented at: Laboratory Animal Sciences Virtual Event Series 2020
    1
     
     
  • MAY 14, 2020 | 12:00 AM
    Evaluation of Analgesia Efficacy in Postoperative Mice Using Novel Cage-Side Measures
    Evaluation of Analgesia Efficacy in Postoperative Mice Using Novel...
    The ability to rapidly and accurately identify pain in mice is critical for providing them optimal care and welfare. To meet this need, we developed and validated the Grooming Transfer Test...
    Speaker: Jennifer Lofgren, DVM, MS, DACLAM
    Presented at: Laboratory Animal Sciences Virtual Event Series 2020
    0
    MAY 12, 2020 | 9:00 AM
    Analytical Quality Control for the Pharmaceutical Industry
    Analytical Quality Control for the Pharmaceutical Industry
    NEW DATE: Date: May 12, 2020 Time: 9:00am PDT, 12:00pm EDT...
    Speaker: Joy McElroy
    Sponsored By: Sartorius
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    MAY 07, 2020 | 7:00 AM
    A complete workflow for analysis of cfDNA: from plasma to variants
    A complete workflow for analysis of cfDNA: from plasma to variants
    DATE: May 7, 2020 TIME: 7:00am PT As cost of sequencing continues to drop, the throughput and complexity of NGS assays have risen precipitously. At the same time, the types of samples being...
    Speaker: Shilpa Parakh , Nicole Roseman
    Sponsored By: Beckman Coulter Life Sciences, Integrated DNA Technologies (IDT)
    13 10
    APR 30, 2020 | 7:00 AM
    Nucleic Acid Purification - Understand the details, tackle the routines!
    Nucleic Acid Purification - Understand the details, tackle the...
    DATE: April 30, 2020 TIME: 7am PT, 10am ET, 4pm CEST Aged more than one century, nucleic acid purification is anything but ordinary. Nucleic Acid Purification, also termed Nucleic Acid Extra...
    Speaker: Andreas von Bohl, PhD , Milan Dieris, PhD , Tim Schommartz, PhD
    Sponsored By: Eppendorf, Macherey-Nagel
    45 13
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