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    Cell Biology

    Cell Biology: a branch of biology that studies the different structures and functions of the cell and focuses mainly on the idea of the cell as the basic unit of life. Cell biology explains the structure, organization of the organelles they contain, their physiological properties, metabolic processes, Signaling pathways, life cycle, and interactions with their environment. This is done both on a microscopic and molecular level as it encompasses prokaryotic cells and eukaryotic cells.

    Webinars

    • OCT 12, 2021 | 8:00 AM
      The growing concern of off-target effects: How to measure and minimize off-target effects in your genome editing experiments.
      The growing concern of off-target effects: How to measure and...
      Date: October 12, 2021 Time: 8:00am (PDT), 11:00am (EDT) CRISPR-Cas9 is a nuclease-based genome editing system which has seen exponential growth in adoption with broad applications from basi...
      Speaker: Pandhita Pochanard
      Sponsored By: Thermo Fisher Scientific/Gibco
      30
      OCT 07, 2021 | 7:00 AM
      Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis
      Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common...
      DATE: October 7, 2021 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CET The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of...
      Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
      Sponsored By: Eppendorf, Promega
      12
      SEP 30, 2021 | 9:00 AM
      Understanding the How and the Why: Biosafety in Centrifugation
      Understanding the How and the Why: Biosafety in Centrifugation
      Date: September 30, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Centrifugation is an indispensable laboratory application for separating and purifying a range of biological materials. Although a...
      Speaker: Dr. Lori Kelman , Robert (Bob) Rodriguez
      Sponsored By: Beckman Coulter Life Sciences
      33
      SEP 29, 2021 | 12:00 PM
      How Can You Characterize Your CRISPR Gene Edits Faster and Easier?
      How Can You Characterize Your CRISPR Gene Edits Faster and Easier?
      Over the past ten years, CRISPR technology has revolutionized genome engineering, as reflected by the recently awarded Nobel Prize. Nevertheless, the process of performing and characterizing...
      Speaker: Domenica Martorana, PhD
      Presented at: CRISPR Virtual Event Series 2021
      Sponsored By: QIAGEN
      1
      SEP 29, 2021 | 9:00 AM
      Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome Engineering
      Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome...
      RNA plays important and diverse roles in biology, but molecular tools to manipulate and measure RNA are limited. We demonstrate that RNA-targeting CRISPR effector Cas13 and novel RNA targeti...
      Speaker: Omar Abudayyeh, PhD , Jonathan Gootenberg, PhD
      Presented at: CRISPR Virtual Event Series 2021
      1
      SEP 28, 2021 | 9:00 AM
      New method for standardized, high-throughput PBMC isolation from blood samples for observational and clinical studies
      New method for standardized, high-throughput PBMC isolation from...
      Date: September 28, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Standardization and reproducibility are prerequisites for peripheral blood mononuclear cell (PBMC) generation from donor or patient...
      Speaker: Lotta Raty , Dr. Steffen Rapp , PhD
      Sponsored By: Miltenyi Biotec
      10 1
      SEP 28, 2021 | 8:00 AM
      New fixable viability dyes and applications for flow cytometry
      New fixable viability dyes and applications for flow cytometry
      Date: September 28, 2021 Time: 8:00am (PDT), 11:00am (EDT) Flow cytometry provides many advantages including single-cell quantitative analysis, high sample throughput, and multiplex cell cha...
      Speaker: Chris Langsdorf
      Sponsored By: Thermo Fisher Scientific
      9 4
      SEP 23, 2021 | 8:00 AM
      Automated surveillance for deep characterization of the tumor microenvironment using ultra-high content imaging
      Automated surveillance for deep characterization of the tumor...
      Date: September 23, 2021 Time: 8:00am (PDT), 11:00am (EDT) The tumor microenvironment (TME) comprises complex cellular and structural features. The processes and interactions within the TME...
      Speaker: Dr. Telma Santos , Dr. Nizar Yousif
      Sponsored By: Miltenyi Biotec
      3 1
      SEP 22, 2021 | 8:00 AM
      Cell therapy needs a data-driven and certified cleanroom-compatible COâ‚‚ incubator
      Cell therapy needs a data-driven and certified cleanroom-compatible...
      Date: September 22, 2021 Time: 8:00am (PDT), 11:00am (EDT) New technologies generating successful cell and gene therapy approaches have contributed to a global growth of institutions that cu...
      Speaker: Mary Kay Bates, M.S.
      Sponsored By: Thermo Fisher Scientific
      6 1
      SEP 22, 2021 | 7:30 AM
      Panel Presentation: Concept-to-Commercialization: Thermo Fisher's Unique Approach to Rapidly Advancing Your Antibody and Protein Therapeutics
      Panel Presentation: Concept-to-Commercialization: Thermo Fisher's...
      Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
      Speaker: Jeff Hou, PhD , Steve Lewis
      Presented at: Cell Biology Virtual Event Series 2021
      Sponsored By: Thermo Fisher Scientific
      0
      SEP 17, 2021 | 12:00 PM
      Developing better techniques for 3D culture and analysis.
      Developing better techniques for 3D culture and analysis.
      Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
      Speaker: Edwin Golez, M.S. , Bhaskar Mandavilli
      Sponsored By: Thermo Fisher Scientific/Gibco
      10 2
      SEP 16, 2021 | 9:00 AM
      Sample Collection and Transport in the Age of COVID: A Retrospective - What Have We Learned.
      Sample Collection and Transport in the Age of COVID: A Retrospective...
      Date: September 16, 2021 Time: 9:00am (PDT), 12:00pm (EDT) For diagnostic testing, proper sample collection and transport play a pivotal role in the accurate and timely identification of a p...
      Speaker: Susan Sharp, Ph.D., DABMM, FAAM
      Sponsored By: COPAN Diagnostics
      7
      SEP 16, 2021 | 8:00 AM
      Concept-to-Commercialization: Thermo Fisher's unique approach to rapidly advancing your antibody and protein therapeutics
      Concept-to-Commercialization: Thermo Fisher's unique approach...
      Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
      Speaker: Jeff Hou, PhD , Steve Lewis
      Sponsored By: Thermo Fisher Scientific
      1
      SEP 15, 2021 | 7:00 AM
      High Parameter Sorting and Analysis using the Bigfoot Cell Sorter
      High Parameter Sorting and Analysis using the Bigfoot Cell Sorter
      Date: September 15, 2021 Time: 7:00am (PDT), 10:00am (EDT) Flow cytometry has become ubiquitous in its applications ranging from research institutions to clinical laboratories. High-dimensio...
      Speaker: Alan Saluk
      Sponsored By: Thermo Fisher Scientific
      3
      SEP 14, 2021 | 7:00 AM
      What prevents you from increasing your PCR productivity?
      What prevents you from increasing your PCR productivity?
      Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
      Speaker: Arora Phang, PhD
      Sponsored By: Eppendorf
      24 1
      SEP 09, 2021 | 10:30 AM
      Approaches to SARS-CoV-2 Sample Processing for RNA Detection
      Approaches to SARS-CoV-2 Sample Processing for RNA Detection
      The ongoing COVID-19 pandemic has elevated the need for reliable, cost-effective means for widespread virus detection. This webinar will explore two distinct approaches for isolating viral R...
      Speaker: Nicolas Caffarelli , Kan Saito, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      Sponsored By: MilliporeSigma
      5
      SEP 09, 2021 | 8:00 AM
      Automated GMP-compliant PSC banking in a closed system
      Automated GMP-compliant PSC banking in a closed system
      Date: September 09, 2021 Time: 8:00am (PDT), 11:00am (EDT) Human pluripotent stem cells (PSCs) hold great potentials in regenerative therapies. In this webinar, Chao will first present the c...
      Speaker: Dr. Chao Sheng , Dr. Rafal Krol
      Sponsored By: Miltenyi Biotec
      7
      SEP 08, 2021 | 9:00 AM
      The surface topography of silicone breast implants mediates the foreign body response in mice, rabbits, and humans
      The surface topography of silicone breast implants mediates the...
      Date: September 8, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Implanted biomedical devices comprise a major component of modern medicine and are essential for many clinical applications ranging...
      Speaker: Dr. Joshua Doloff
      Sponsored By: Thermo Fisher Scientific
      8
      SEP 08, 2021 | 12:00 AM
      Memory CD4 T cells established by endemic human Coronaviruses have the potential to be recruited into the response to SARS-Cov-2
      Memory CD4 T cells established by endemic human Coronaviruses...
      For centuries, humans have become infected with endemic circulating Coronaviruses (hCoV), typically repeatedly throughout life. To understand how these respiratory infections establish CD4 T...
      Speaker: Andrea J. Sant, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      0
      SEP 08, 2021 | 12:00 AM
      Development of Self-Replicating Propagation-Defective RNAs as Next Generation Vaccine Candidates Against Human Coronaviruses
      Development of Self-Replicating Propagation-Defective RNAs as...
      Self-amplifying RNA replicons are promising candidates for next generation vaccines against human coronaviruses with pandemic potential. Self-amplification of RNAs in host cells generates mo...
      Speaker: Isabel Sola, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      0
      SEP 08, 2021 | 12:00 AM
      Genetic Approaches and Host Targets to Inhibit Influenza Virus Pathogenesis
      Genetic Approaches and Host Targets to Inhibit Influenza Virus...
      Influenza viruses represent a significant burden to human health. In order to limit respiratory viral disease, new approaches that can prevent viral infection and spread are needed. We have...
      Speaker: Nicholas Heaton, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      0
      AUG 31, 2021 | 9:00 AM
      The Regulation of ALC1 by TRIM33 during the DNA Damage Response
      The Regulation of ALC1 by TRIM33 during the DNA Damage Response
      Date: July 07, 2021 Time: 8:00am (PDT), 11:00am (EDT) The DNA damage response is extremely crucial in maintaining genomic integrity. Failure to repair damaged DNA can result in the propagati...
      Speaker: Rukia Henry
      Sponsored By: Thermo Fisher Scientific/Gibco
      4
      AUG 26, 2021 | 7:00 AM
      Deciphering the cross-talk between immunosuppressive cells in pancreatic cancer using 3D light sheet microscopy
      Deciphering the cross-talk between immunosuppressive cells in...
      Date: August 26, 2021 Time: 7:00am (PDT), 10:00am (EDT) Pancreatic ductal adenocarcinoma (PDAC) is a fatal disease with rising incidence and a remarkable resistance to current therapies. In t...
      Speaker: Carole Siret, Ph.D.
      Sponsored By: Miltenyi Biotec
      9
      AUG 25, 2021 | 1:30 PM
      Spatial Context Matters: Molecular Cartographyâ„¢ to Study Gene Expression Changes in the Brain
      Spatial Context Matters: Molecular Cartographyâ„¢ to Study Gene...
      Technological advances such as single-cell RNA sequencing accelerated our understanding of cellular diversity in tissues. However, the ability to elucidate this cellular heterogeneity while...
      Speaker: Jeroen Aerts, PhD
      Presented at: Neuroscience Virtual Event Series 2021
      Sponsored By: Resolve Biosciences
      1
    OCT 12, 2021 | 8:00 AM
    The growing concern of off-target effects: How to measure and minimize off-target effects in your genome editing experiments.
    The growing concern of off-target effects: How to measure and...
    Date: October 12, 2021 Time: 8:00am (PDT), 11:00am (EDT) CRISPR-Cas9 is a nuclease-based genome editing system which has seen exponential growth in adoption with broad applications from basi...
    Speaker: Pandhita Pochanard
    Sponsored By: Thermo Fisher Scientific/Gibco
    30
    OCT 07, 2021 | 7:00 AM
    Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis
    Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common...
    DATE: October 7, 2021 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CET The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of...
    Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
    Sponsored By: Eppendorf, Promega
    12
    SEP 30, 2021 | 9:00 AM
    Understanding the How and the Why: Biosafety in Centrifugation
    Understanding the How and the Why: Biosafety in Centrifugation
    Date: September 30, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Centrifugation is an indispensable laboratory application for separating and purifying a range of biological materials. Although a...
    Speaker: Dr. Lori Kelman , Robert (Bob) Rodriguez
    Sponsored By: Beckman Coulter Life Sciences
    33
    SEP 29, 2021 | 12:00 PM
    How Can You Characterize Your CRISPR Gene Edits Faster and Easier?
    How Can You Characterize Your CRISPR Gene Edits Faster and Easier?
    Over the past ten years, CRISPR technology has revolutionized genome engineering, as reflected by the recently awarded Nobel Prize. Nevertheless, the process of performing and characterizing...
    Speaker: Domenica Martorana, PhD
    Presented at: CRISPR Virtual Event Series 2021
    Sponsored By: QIAGEN
    1
    SEP 29, 2021 | 9:00 AM
    Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome Engineering
    Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome...
    RNA plays important and diverse roles in biology, but molecular tools to manipulate and measure RNA are limited. We demonstrate that RNA-targeting CRISPR effector Cas13 and novel RNA targeti...
    Speaker: Omar Abudayyeh, PhD , Jonathan Gootenberg, PhD
    Presented at: CRISPR Virtual Event Series 2021
    1
     
     
  • SEP 28, 2021 | 9:00 AM
    New method for standardized, high-throughput PBMC isolation from blood samples for observational and clinical studies
    New method for standardized, high-throughput PBMC isolation from...
    Date: September 28, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Standardization and reproducibility are prerequisites for peripheral blood mononuclear cell (PBMC) generation from donor or patient...
    Speaker: Lotta Raty , Dr. Steffen Rapp , PhD
    Sponsored By: Miltenyi Biotec
    10 1
    SEP 28, 2021 | 8:00 AM
    New fixable viability dyes and applications for flow cytometry
    New fixable viability dyes and applications for flow cytometry
    Date: September 28, 2021 Time: 8:00am (PDT), 11:00am (EDT) Flow cytometry provides many advantages including single-cell quantitative analysis, high sample throughput, and multiplex cell cha...
    Speaker: Chris Langsdorf
    Sponsored By: Thermo Fisher Scientific
    9 4
    SEP 23, 2021 | 8:00 AM
    Automated surveillance for deep characterization of the tumor microenvironment using ultra-high content imaging
    Automated surveillance for deep characterization of the tumor...
    Date: September 23, 2021 Time: 8:00am (PDT), 11:00am (EDT) The tumor microenvironment (TME) comprises complex cellular and structural features. The processes and interactions within the TME...
    Speaker: Dr. Telma Santos , Dr. Nizar Yousif
    Sponsored By: Miltenyi Biotec
    3 1
    SEP 22, 2021 | 8:00 AM
    Cell therapy needs a data-driven and certified cleanroom-compatible COâ‚‚ incubator
    Cell therapy needs a data-driven and certified cleanroom-compatible...
    Date: September 22, 2021 Time: 8:00am (PDT), 11:00am (EDT) New technologies generating successful cell and gene therapy approaches have contributed to a global growth of institutions that cu...
    Speaker: Mary Kay Bates, M.S.
    Sponsored By: Thermo Fisher Scientific
    6 1
    SEP 22, 2021 | 7:30 AM
    Panel Presentation: Concept-to-Commercialization: Thermo Fisher's Unique Approach to Rapidly Advancing Your Antibody and Protein Therapeutics
    Panel Presentation: Concept-to-Commercialization: Thermo Fisher's...
    Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
    Speaker: Jeff Hou, PhD , Steve Lewis
    Presented at: Cell Biology Virtual Event Series 2021
    Sponsored By: Thermo Fisher Scientific
    0
     
     
  • SEP 17, 2021 | 12:00 PM
    Developing better techniques for 3D culture and analysis.
    Developing better techniques for 3D culture and analysis.
    Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
    Speaker: Edwin Golez, M.S. , Bhaskar Mandavilli
    Sponsored By: Thermo Fisher Scientific/Gibco
    10 2
    SEP 16, 2021 | 9:00 AM
    Sample Collection and Transport in the Age of COVID: A Retrospective - What Have We Learned.
    Sample Collection and Transport in the Age of COVID: A Retrospective...
    Date: September 16, 2021 Time: 9:00am (PDT), 12:00pm (EDT) For diagnostic testing, proper sample collection and transport play a pivotal role in the accurate and timely identification of a p...
    Speaker: Susan Sharp, Ph.D., DABMM, FAAM
    Sponsored By: COPAN Diagnostics
    7
    SEP 16, 2021 | 8:00 AM
    Concept-to-Commercialization: Thermo Fisher's unique approach to rapidly advancing your antibody and protein therapeutics
    Concept-to-Commercialization: Thermo Fisher's unique approach...
    Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
    Speaker: Jeff Hou, PhD , Steve Lewis
    Sponsored By: Thermo Fisher Scientific
    1
    SEP 15, 2021 | 7:00 AM
    High Parameter Sorting and Analysis using the Bigfoot Cell Sorter
    High Parameter Sorting and Analysis using the Bigfoot Cell Sorter
    Date: September 15, 2021 Time: 7:00am (PDT), 10:00am (EDT) Flow cytometry has become ubiquitous in its applications ranging from research institutions to clinical laboratories. High-dimensio...
    Speaker: Alan Saluk
    Sponsored By: Thermo Fisher Scientific
    3
    SEP 14, 2021 | 7:00 AM
    What prevents you from increasing your PCR productivity?
    What prevents you from increasing your PCR productivity?
    Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
    Speaker: Arora Phang, PhD
    Sponsored By: Eppendorf
    24 1
     
     
  • SEP 09, 2021 | 10:30 AM
    Approaches to SARS-CoV-2 Sample Processing for RNA Detection
    Approaches to SARS-CoV-2 Sample Processing for RNA Detection
    The ongoing COVID-19 pandemic has elevated the need for reliable, cost-effective means for widespread virus detection. This webinar will explore two distinct approaches for isolating viral R...
    Speaker: Nicolas Caffarelli , Kan Saito, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    Sponsored By: MilliporeSigma
    5
    SEP 09, 2021 | 8:00 AM
    Automated GMP-compliant PSC banking in a closed system
    Automated GMP-compliant PSC banking in a closed system
    Date: September 09, 2021 Time: 8:00am (PDT), 11:00am (EDT) Human pluripotent stem cells (PSCs) hold great potentials in regenerative therapies. In this webinar, Chao will first present the c...
    Speaker: Dr. Chao Sheng , Dr. Rafal Krol
    Sponsored By: Miltenyi Biotec
    7
    SEP 08, 2021 | 9:00 AM
    The surface topography of silicone breast implants mediates the foreign body response in mice, rabbits, and humans
    The surface topography of silicone breast implants mediates the...
    Date: September 8, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Implanted biomedical devices comprise a major component of modern medicine and are essential for many clinical applications ranging...
    Speaker: Dr. Joshua Doloff
    Sponsored By: Thermo Fisher Scientific
    8
    SEP 08, 2021 | 12:00 AM
    Memory CD4 T cells established by endemic human Coronaviruses have the potential to be recruited into the response to SARS-Cov-2
    Memory CD4 T cells established by endemic human Coronaviruses...
    For centuries, humans have become infected with endemic circulating Coronaviruses (hCoV), typically repeatedly throughout life. To understand how these respiratory infections establish CD4 T...
    Speaker: Andrea J. Sant, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    0
    SEP 08, 2021 | 12:00 AM
    Development of Self-Replicating Propagation-Defective RNAs as Next Generation Vaccine Candidates Against Human Coronaviruses
    Development of Self-Replicating Propagation-Defective RNAs as...
    Self-amplifying RNA replicons are promising candidates for next generation vaccines against human coronaviruses with pandemic potential. Self-amplification of RNAs in host cells generates mo...
    Speaker: Isabel Sola, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    0
     
     
  • SEP 08, 2021 | 12:00 AM
    Genetic Approaches and Host Targets to Inhibit Influenza Virus Pathogenesis
    Genetic Approaches and Host Targets to Inhibit Influenza Virus...
    Influenza viruses represent a significant burden to human health. In order to limit respiratory viral disease, new approaches that can prevent viral infection and spread are needed. We have...
    Speaker: Nicholas Heaton, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    0
    AUG 31, 2021 | 9:00 AM
    The Regulation of ALC1 by TRIM33 during the DNA Damage Response
    The Regulation of ALC1 by TRIM33 during the DNA Damage Response
    Date: July 07, 2021 Time: 8:00am (PDT), 11:00am (EDT) The DNA damage response is extremely crucial in maintaining genomic integrity. Failure to repair damaged DNA can result in the propagati...
    Speaker: Rukia Henry
    Sponsored By: Thermo Fisher Scientific/Gibco
    4
    AUG 26, 2021 | 7:00 AM
    Deciphering the cross-talk between immunosuppressive cells in pancreatic cancer using 3D light sheet microscopy
    Deciphering the cross-talk between immunosuppressive cells in...
    Date: August 26, 2021 Time: 7:00am (PDT), 10:00am (EDT) Pancreatic ductal adenocarcinoma (PDAC) is a fatal disease with rising incidence and a remarkable resistance to current therapies. In t...
    Speaker: Carole Siret, Ph.D.
    Sponsored By: Miltenyi Biotec
    9
    AUG 25, 2021 | 1:30 PM
    Spatial Context Matters: Molecular Cartographyâ„¢ to Study Gene Expression Changes in the Brain
    Spatial Context Matters: Molecular Cartographyâ„¢ to Study Gene...
    Technological advances such as single-cell RNA sequencing accelerated our understanding of cellular diversity in tissues. However, the ability to elucidate this cellular heterogeneity while...
    Speaker: Jeroen Aerts, PhD
    Presented at: Neuroscience Virtual Event Series 2021
    Sponsored By: Resolve Biosciences
    1
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