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    Primary Culture

    Primary Culture: the stage of the culture after the cells are isolated from the tissue and proliferated under the appropriate conditions until they occupy all of the available substrate.

    Webinars

    • MAY 06, 2021 | 7:00 AM
      Everyday Cell Culture Practice - Improve reproducibility of your cell culture
      Everyday Cell Culture Practice - Improve reproducibility of your...
      Date: May 6, 2021 Time: 7:00am PDT, 10:00am EDT, 4:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal changes in cultivati...
      Speaker: Jessica Wagener, PhD
      Sponsored By: Eppendorf
      29
      MAR 18, 2021 | 8:00 AM
      Facilitating transcriptomics of sensitive target cells with gentle cell sorting
      Facilitating transcriptomics of sensitive target cells with gentle...
      DATE: March 18, 2021 TIME: 8:00am PDT Sequencing of bulk cells, single cells, and nuclei is opening doors in the understanding of complex biological processes....
      Speaker: Alexander Jonsson , Dr. Alina Steinbach
      Sponsored By: Miltenyi Biotec
      11
      FEB 24, 2021 | 10:00 AM
      Biosafety Enclosures and the Automated Lab: Steps for Success
      Biosafety Enclosures and the Automated Lab: Steps for Success
      DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
      Speaker: David Wasescha , Simon Fogarty
      Sponsored By: Labconco
      15
      FEB 24, 2021 | 12:00 AM
      Preclinical evaluation of novel OT-analogs for the potential treatment of social behavioral deficits
      Preclinical evaluation of novel OT-analogs for the potential treatment...
      As pharmaceutical companies have reduced or eliminated neuroscience drug development due to high costs and low approval rates, the economic and healthcare burden of CNS disorders in the Unit...
      Speaker: Marsha Pierce, PhD
      Presented at: Drug Discovery & Development Virtual Event Series 2021
      2
      FEB 04, 2021 | 8:00 AM
      Automation of organoid assays requiring a 3D Matrix with dragonfly® discovery
      Automation of organoid assays requiring a 3D Matrix with dragonfly®...
      Date: February 4, 2021 Time: 8:00am (PST), 11:00am (EST) Over the last several years, there has been increased interest around the use of patient derived organoids for personalized medicine...
      Speaker: Hilary Sherman
      Sponsored By: SPT Labtech
      8
      DEC 17, 2020 | 9:00 AM
      Advancing Cell Biology with Cryo-Correlative Microscopy
      Advancing Cell Biology with Cryo-Correlative Microscopy
      Date: December 17, 2020 Time: 9:00am (PST), 12:00pm (EST) Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging moda...
      Speaker: Elizabeth Wright, Ph.D. , Jae Yang, Ph.D. , Bryan Sibert, Ph.D. , Joseph Kim
      Sponsored By: Leica Microsystems, Alveole
      13 2
      DEC 09, 2020 | 9:00 AM
      Human hematopoietic stem cell research made smart and easy
      Human hematopoietic stem cell research made smart and easy
      DATE: December 09, 2020 TIME: 09:00am PST Hematopoietic stem cells (HSCs) ensure homeostasis of blood throughout life. In this webinar, we share insights into the smart and easy isolation, c...
      Speaker: Antje Kremer
      Sponsored By: Miltenyi Biotec
      15 1
      DEC 02, 2020 | 8:00 AM
      Optimize your bioprocess - From shaker to bioreactor
      Optimize your bioprocess - From shaker to bioreactor
      DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
      Speaker: Ines Hartmann , David Solbach
      Sponsored By: Eppendorf
      11
      NOV 18, 2020 | 7:00 AM
      Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis
      Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common...
      DATE: November 18, 2020 TIME: 7:00am PST, 10:00am PST, 4:00pm CEST The assessment of cell health and cellular responses after experimental manipulation continue to be a very important......
      Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
      Sponsored By: Eppendorf, Promega
      17
      NOV 05, 2020 | 7:00 AM
      Stem cell derived exosome production in stirred-tank bioreactors
      Stem cell derived exosome production in stirred-tank bioreactors
      DATE: Date needed, 2020 TIME: Time needed Exosomes are a population of naturally occurring mobile, membrane-limited, 30 – 100 nm in diameter, extracellular vesicles containing a large...
      Speaker: Jorge Escobar , Aurélie Tacheny
      Presented at: Eppendorf Planet of Knowledge - A New Dimension
      Sponsored By: Eppendorf
      12
      OCT 09, 2020 | 8:00 AM
      The Expanding Role of Diagnostic Next Generation Sequencing (NGS) in the Clinical Microbiology Laboratory
      The Expanding Role of Diagnostic Next Generation Sequencing (NGS)...
      DATE: October 09, 2020 TIME: 08:00am PT Traditional culture techniques, as the primary diagnostic tool for infectious diseases, are coming under scrutiny for failing to universally detect in...
      Speaker: Joseph John, Jr., MD, FIDSA , Michael Schmidt, Ph.D. FAAM, FACD
      Sponsored By: MicroGenDX
      16
      OCT 08, 2020 | 7:00 AM
      Protect your cells with proper pipetting - How liquid handling influences your cell culture work
      Protect your cells with proper pipetting - How liquid handling...
      DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
      Speaker: Nadine Mellies, PhD , Jessica Wagener, PhD
      Sponsored By: Eppendorf
      33 5
      OCT 07, 2020 | 7:30 AM
      How to derive and expand primary tumor cell cultures
      How to derive and expand primary tumor cell cultures
      This webinar covers our latest solutions for improved culture of primary and xenotransplanted tumor cells. Get insights into dissociation of fresh tumor samples into viable single-cell suspe...
      Speaker: David J. Agorku
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
      Sponsored By: Miltenyi Biotec
      2
      SEP 23, 2020 | 3:00 PM
      Beyond the Culture Flask: How Membrane- and Microfluidic-based Platforms can create more Physiologically Relevant Tissue Culture Systems
      Beyond the Culture Flask: How Membrane- and Microfluidic-based...
      For more than a century, breakthroughs in biological sciences have relied on the ability to study cells outside of respective organisms. While majority of cell culturing is still performed u...
      Speaker: Jun Park, PhD
      Presented at: Cell Biology Virtual Event Series 2020
      Sponsored By: MilliporeSigma
      1
      SEP 23, 2020 | 10:30 AM
      iPSC- and patient- derived intestinal organoids for gastrointestinal research
      iPSC- and patient- derived intestinal organoids for gastrointestinal...
      Intestinal organoids are self-organizing, 3D structures derived from either pluripotent stem cells or from primary tissues with the abiltiy to recapitulate some of the spatial architecture a...
      Speaker: Kevin Su
      Presented at: Cell Biology Virtual Event Series 2020
      Sponsored By: MilliporeSigma
      2
      SEP 23, 2020 | 8:00 AM
      CAR T-Cell Immunotherapy of Solid Tumors - Learning from the Clinic and Lab in Parallel
      CAR T-Cell Immunotherapy of Solid Tumors - Learning from the Clinic...
      Date: Time: Over the last couple of years, it has become apparent that affinity-tuned CAR T cells display enhanced expansion and prolonged persistence in patients and that this strategy can...
      Speaker: John Maher, PhD , Rogier Reijmers, PhD
      LUMICKS
      9 1
      AUG 25, 2020 | 8:00 AM
      How LV and AAV Transfection Platforms will Shape the Future of Gene Therapy Manufacturing
      How LV and AAV Transfection Platforms will Shape the Future of...
      DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
      Speaker: Leisha Kopp
      Sponsored By: Mirus
      18 2
      JUN 30, 2020 | 9:00 AM
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
      DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
      Speaker: Jane Weitzel
      Sponsored By: Sartorius
      17 1
      JUN 24, 2020 | 8:00 AM
      CRISPR-Cas assisted genetics in intestinal organoids
      CRISPR-Cas assisted genetics in intestinal organoids
      DATE: June 24, 2020 TIME: 8:00am PT The identification of LGR5 + intestinal stem cells helped us to understand various aspects of adult stem cells and led to the establishment of primary 3D...
      Speaker: Bon-Kyoung Koo, PhD
      Sponsored By: GenScript
      33 5
      JUN 18, 2020 | 9:00 AM
      Unveiling the role of T cell dysfunction in the development and progression of glioblastoma
      Unveiling the role of T cell dysfunction in the development and...
      DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...
      Speaker: Felipe Galvez Cancino, Ph.D.
      Sponsored By: Thermo Fisher Scientific/Gibco
      18 2
      JUN 09, 2020 | 6:00 AM
      Smart Plastics: Improving qPCR reliability & reproducibility
      Smart Plastics: Improving qPCR reliability & reproducibility
      DATE: June 9, 2020 TIME: 6am PT, 9am ET, 3pm CEST The importance of disposable plastic consumables and their overall impact on the experimental workflow of qPCR has been taken into considera...
      Speaker: Michael Pfaffl, Ph.D. , Tim Schommartz, PhD , Rafal Grzeskowiak, PhD
      Sponsored By: Eppendorf
      39
      MAY 13, 2020 | 7:00 AM
      Stem cell culture: Make or break - Find the optimum for your MSCs and PSCs
      Stem cell culture: Make or break - Find the optimum for your MSCs...
      DATE: May 13, 2020 TIME: 7am PT, 10am ET, 4pm CEST Stem cells represent an important tool in a wide range of applications, including basic research, disease modeling, drug discovery, and reg...
      Speaker: Jessica Wagener, PhD , Nadine Mellies, PhD
      Sponsored By: Eppendorf
      37
      MAY 12, 2020 | 9:00 AM
      Analytical Quality Control for the Pharmaceutical Industry
      Analytical Quality Control for the Pharmaceutical Industry
      NEW DATE: Date: May 12, 2020 Time: 9:00am PDT, 12:00pm EDT...
      Speaker: Joy McElroy
      Sponsored By: Sartorius
      29 9
      MAY 07, 2020 | 8:00 AM
      When Cell Growth is Critical: Evaluating CO₂ Incubator Parameters for Cell Therapy
      When Cell Growth is Critical: Evaluating CO₂ Incubator Parameters...
      DATE: May 7, 2020 TIME: 8:00AM PDT, 11:00AM EDT With the recent explosion of cell therapy, we know more about conditions affecting cell growth than ever before. Your CO2 incubator should not...
      Speaker: Mary Kay Bates, M.S.
      Sponsored By: Thermo Fisher Scientific
      15 2
    MAY 06, 2021 | 7:00 AM
    Everyday Cell Culture Practice - Improve reproducibility of your cell culture
    Everyday Cell Culture Practice - Improve reproducibility of your...
    Date: May 6, 2021 Time: 7:00am PDT, 10:00am EDT, 4:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal changes in cultivati...
    Speaker: Jessica Wagener, PhD
    Sponsored By: Eppendorf
    29
    MAR 18, 2021 | 8:00 AM
    Facilitating transcriptomics of sensitive target cells with gentle cell sorting
    Facilitating transcriptomics of sensitive target cells with gentle...
    DATE: March 18, 2021 TIME: 8:00am PDT Sequencing of bulk cells, single cells, and nuclei is opening doors in the understanding of complex biological processes....
    Speaker: Alexander Jonsson , Dr. Alina Steinbach
    Sponsored By: Miltenyi Biotec
    11
    FEB 24, 2021 | 10:00 AM
    Biosafety Enclosures and the Automated Lab: Steps for Success
    Biosafety Enclosures and the Automated Lab: Steps for Success
    DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
    Speaker: David Wasescha , Simon Fogarty
    Sponsored By: Labconco
    15
    FEB 24, 2021 | 12:00 AM
    Preclinical evaluation of novel OT-analogs for the potential treatment of social behavioral deficits
    Preclinical evaluation of novel OT-analogs for the potential treatment...
    As pharmaceutical companies have reduced or eliminated neuroscience drug development due to high costs and low approval rates, the economic and healthcare burden of CNS disorders in the Unit...
    Speaker: Marsha Pierce, PhD
    Presented at: Drug Discovery & Development Virtual Event Series 2021
    2
    FEB 04, 2021 | 8:00 AM
    Automation of organoid assays requiring a 3D Matrix with dragonfly® discovery
    Automation of organoid assays requiring a 3D Matrix with dragonfly®...
    Date: February 4, 2021 Time: 8:00am (PST), 11:00am (EST) Over the last several years, there has been increased interest around the use of patient derived organoids for personalized medicine...
    Speaker: Hilary Sherman
    Sponsored By: SPT Labtech
    8
     
     
  • DEC 17, 2020 | 9:00 AM
    Advancing Cell Biology with Cryo-Correlative Microscopy
    Advancing Cell Biology with Cryo-Correlative Microscopy
    Date: December 17, 2020 Time: 9:00am (PST), 12:00pm (EST) Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging moda...
    Speaker: Elizabeth Wright, Ph.D. , Jae Yang, Ph.D. , Bryan Sibert, Ph.D. , Joseph Kim
    Sponsored By: Leica Microsystems, Alveole
    13 2
    DEC 09, 2020 | 9:00 AM
    Human hematopoietic stem cell research made smart and easy
    Human hematopoietic stem cell research made smart and easy
    DATE: December 09, 2020 TIME: 09:00am PST Hematopoietic stem cells (HSCs) ensure homeostasis of blood throughout life. In this webinar, we share insights into the smart and easy isolation, c...
    Speaker: Antje Kremer
    Sponsored By: Miltenyi Biotec
    15 1
    DEC 02, 2020 | 8:00 AM
    Optimize your bioprocess - From shaker to bioreactor
    Optimize your bioprocess - From shaker to bioreactor
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    Speaker: Ines Hartmann , David Solbach
    Sponsored By: Eppendorf
    11
    NOV 18, 2020 | 7:00 AM
    Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis
    Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common...
    DATE: November 18, 2020 TIME: 7:00am PST, 10:00am PST, 4:00pm CEST The assessment of cell health and cellular responses after experimental manipulation continue to be a very important......
    Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
    Sponsored By: Eppendorf, Promega
    17
    NOV 05, 2020 | 7:00 AM
    Stem cell derived exosome production in stirred-tank bioreactors
    Stem cell derived exosome production in stirred-tank bioreactors
    DATE: Date needed, 2020 TIME: Time needed Exosomes are a population of naturally occurring mobile, membrane-limited, 30 – 100 nm in diameter, extracellular vesicles containing a large...
    Speaker: Jorge Escobar , Aurélie Tacheny
    Presented at: Eppendorf Planet of Knowledge - A New Dimension
    Sponsored By: Eppendorf
    12
     
     
  • OCT 09, 2020 | 8:00 AM
    The Expanding Role of Diagnostic Next Generation Sequencing (NGS) in the Clinical Microbiology Laboratory
    The Expanding Role of Diagnostic Next Generation Sequencing (NGS)...
    DATE: October 09, 2020 TIME: 08:00am PT Traditional culture techniques, as the primary diagnostic tool for infectious diseases, are coming under scrutiny for failing to universally detect in...
    Speaker: Joseph John, Jr., MD, FIDSA , Michael Schmidt, Ph.D. FAAM, FACD
    Sponsored By: MicroGenDX
    16
    OCT 08, 2020 | 7:00 AM
    Protect your cells with proper pipetting - How liquid handling influences your cell culture work
    Protect your cells with proper pipetting - How liquid handling...
    DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
    Speaker: Nadine Mellies, PhD , Jessica Wagener, PhD
    Sponsored By: Eppendorf
    33 5
    OCT 07, 2020 | 7:30 AM
    How to derive and expand primary tumor cell cultures
    How to derive and expand primary tumor cell cultures
    This webinar covers our latest solutions for improved culture of primary and xenotransplanted tumor cells. Get insights into dissociation of fresh tumor samples into viable single-cell suspe...
    Speaker: David J. Agorku
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
    Sponsored By: Miltenyi Biotec
    2
    SEP 23, 2020 | 3:00 PM
    Beyond the Culture Flask: How Membrane- and Microfluidic-based Platforms can create more Physiologically Relevant Tissue Culture Systems
    Beyond the Culture Flask: How Membrane- and Microfluidic-based...
    For more than a century, breakthroughs in biological sciences have relied on the ability to study cells outside of respective organisms. While majority of cell culturing is still performed u...
    Speaker: Jun Park, PhD
    Presented at: Cell Biology Virtual Event Series 2020
    Sponsored By: MilliporeSigma
    1
    SEP 23, 2020 | 10:30 AM
    iPSC- and patient- derived intestinal organoids for gastrointestinal research
    iPSC- and patient- derived intestinal organoids for gastrointestinal...
    Intestinal organoids are self-organizing, 3D structures derived from either pluripotent stem cells or from primary tissues with the abiltiy to recapitulate some of the spatial architecture a...
    Speaker: Kevin Su
    Presented at: Cell Biology Virtual Event Series 2020
    Sponsored By: MilliporeSigma
    2
     
     
  • SEP 23, 2020 | 8:00 AM
    CAR T-Cell Immunotherapy of Solid Tumors - Learning from the Clinic and Lab in Parallel
    CAR T-Cell Immunotherapy of Solid Tumors - Learning from the Clinic...
    Date: Time: Over the last couple of years, it has become apparent that affinity-tuned CAR T cells display enhanced expansion and prolonged persistence in patients and that this strategy can...
    Speaker: John Maher, PhD , Rogier Reijmers, PhD
    LUMICKS
    9 1
    AUG 25, 2020 | 8:00 AM
    How LV and AAV Transfection Platforms will Shape the Future of Gene Therapy Manufacturing
    How LV and AAV Transfection Platforms will Shape the Future of...
    DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
    Speaker: Leisha Kopp
    Sponsored By: Mirus
    18 2
    JUN 30, 2020 | 9:00 AM
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
    DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
    Speaker: Jane Weitzel
    Sponsored By: Sartorius
    17 1
    JUN 24, 2020 | 8:00 AM
    CRISPR-Cas assisted genetics in intestinal organoids
    CRISPR-Cas assisted genetics in intestinal organoids
    DATE: June 24, 2020 TIME: 8:00am PT The identification of LGR5 + intestinal stem cells helped us to understand various aspects of adult stem cells and led to the establishment of primary 3D...
    Speaker: Bon-Kyoung Koo, PhD
    Sponsored By: GenScript
    33 5
    JUN 18, 2020 | 9:00 AM
    Unveiling the role of T cell dysfunction in the development and progression of glioblastoma
    Unveiling the role of T cell dysfunction in the development and...
    DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...
    Speaker: Felipe Galvez Cancino, Ph.D.
    Sponsored By: Thermo Fisher Scientific/Gibco
    18 2
     
     
  • JUN 09, 2020 | 6:00 AM
    Smart Plastics: Improving qPCR reliability & reproducibility
    Smart Plastics: Improving qPCR reliability & reproducibility
    DATE: June 9, 2020 TIME: 6am PT, 9am ET, 3pm CEST The importance of disposable plastic consumables and their overall impact on the experimental workflow of qPCR has been taken into considera...
    Speaker: Michael Pfaffl, Ph.D. , Tim Schommartz, PhD , Rafal Grzeskowiak, PhD
    Sponsored By: Eppendorf
    39
    MAY 13, 2020 | 7:00 AM
    Stem cell culture: Make or break - Find the optimum for your MSCs and PSCs
    Stem cell culture: Make or break - Find the optimum for your MSCs...
    DATE: May 13, 2020 TIME: 7am PT, 10am ET, 4pm CEST Stem cells represent an important tool in a wide range of applications, including basic research, disease modeling, drug discovery, and reg...
    Speaker: Jessica Wagener, PhD , Nadine Mellies, PhD
    Sponsored By: Eppendorf
    37
    MAY 12, 2020 | 9:00 AM
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    Analytical Quality Control for the Pharmaceutical Industry
    NEW DATE: Date: May 12, 2020 Time: 9:00am PDT, 12:00pm EDT...
    Speaker: Joy McElroy
    Sponsored By: Sartorius
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    MAY 07, 2020 | 8:00 AM
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    DATE: May 7, 2020 TIME: 8:00AM PDT, 11:00AM EDT With the recent explosion of cell therapy, we know more about conditions affecting cell growth than ever before. Your CO2 incubator should not...
    Speaker: Mary Kay Bates, M.S.
    Sponsored By: Thermo Fisher Scientific
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