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    RNA-Seq (RNA sequencing)

    RNA-Seq (RNA sequencing): also called whole transcriptome shotgun sequencing, uses next-generation sequencing to reveal the presence and quantity of RNA in a biological sample at a given moment. RNA-Seq is used to analyze the continuously changing cellular transcriptome.

    Webinars

    • MAR 28, 2024 | 8:00 AM
      Identification of Frequent Autoreactive B-cell Response in Pancreatic Ductal Adenocarcinoma Using Cell Sorting and Single-Cell Sequencing
      Identification of Frequent Autoreactive B-cell Response in Pancreatic...
      B cell responses within pancreatic ductal adenocarcinoma (PDAC) tumors are associated with improved patient outcomes, yet the specific triggering antigens remain largely unknown. In this web...
      Speaker: Min Yao, Ph.D. , Patrick Murphy, Ph.D.
      Sponsored By: Sony Biotechnology
      12
      MAR 26, 2024 | 11:00 AM
      Complete and Comprehensive Biomarker Discovery: TerraFlow for Flow Cytometry Analysis
      Complete and Comprehensive Biomarker Discovery: TerraFlow for...
      Ever wonder what you’re missing in your data? The sheer complexity of today’s flow and mass cytometry datasets demands automated solutions. Machine learning plugins only provide...
      Speaker: Dan Freeman , Pratip K. Chattopadhyay
      Sponsored By: terraFlow Bio
      19
      NOV 14, 2023 | 8:00 AM
      High-throughput RNA-Seq of clinical samples using Covaris AFA ultrasonication
      High-throughput RNA-Seq of clinical samples using Covaris AFA...
      RNA-Seq remains a critical and robust methodology in understanding cellular and organismal changes associated with development and disease. Advances in automation have helped reduce the cost...
      Speaker: Madan Ambavaram, Ph.D. , Stuart Levine, Ph.D.
      Sponsored By: Covaris
      12
      March 23/24 2023
      Advancing the Fight Against Tuberculosis (TB) with Next Generation Sequencing
      Advancing the Fight Against Tuberculosis (TB) with Next Generation...
      Date: 24 March 2023...
      Speaker: Professor Vitali Sintchenko , Dr Timothy Rodwell , Dr Melanie Grobbelaar
      Sponsored By: Illumina
      65
      FEB 14, 2023 | 10:00 AM
      A spatially-resolved transcriptional atlas of the murine dorsal pontine tegmentum
      A spatially-resolved transcriptional atlas of the murine dorsal...
      Date: February 14, 2023 Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CET) The dorsal pontine tegmentum (PnTg) is part of the Pons, the largest section of the brainstem. It is a complex region...
      Speaker: Stefano Nardone, PhD , Cory Brannigan
      Sponsored By: Vizgen
      6
      DEC 13, 2022 | 8:00 AM
      Assessing CRISPR On-Target Editing and Structural Changes with UDiTaSâ„¢ using Tagifyâ„¢ Reagents
      Assessing CRISPR On-Target Editing and Structural Changes with...
      Date: December 13, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeu...
      Speaker: Jack Leonard, PhD , Georgia Giannoukos, PhD
      Sponsored By: seqWell
      13
      APR 19, 2022 | 1:30 PM
      C.E. CREDITS
      Boosting Signal-to-Noise in Single Cell RNA-Seq Through Molecular Depletion
      Boosting Signal-to-Noise in Single Cell RNA-Seq Through Molecular...
      Regardless of method, single cell RNA-seq only captures a small fraction of the transcriptome of each cell. Often, this is due to inherent limitations of the methodology as reads ‘drop...
      Speaker: Dante DeAscanis
      Presented at: Genetics Week Virtual Event Series 2022
      Sponsored By: Jumpcode Genomics
      3
      MAR 02, 2022 | 9:00 AM
      Double transcriptomic reads in single cell RNA-seq using molecular depletion
      Double transcriptomic reads in single cell RNA-seq using molecular...
      Date: March 02, 2022 Time: 9:00am (PST), 12:00pm (EST) Single cell RNA-seq is known to only capture a small fraction of the transcriptome of each cell. Often, this is due to inherent limitat...
      Speaker: Jon Bezney
      Sponsored By: Jumpcode Genomics
      9
      NOV 15, 2021 | 6:00 AM
      Fully automated RNA library preparation for sequencing respiratory viruses
      Fully automated RNA library preparation for sequencing respiratory...
      Date: November 15, 2021 Time: 6:00am (PDT), 9:00am (EDT) Targeted sequencing of respiratory viruses’ genomes is important to identify novel viral variants. Compared to amplicon sequenc...
      Speaker: Dr. Tomasz Zemojtel , Randy Pares
      Sponsored By: Beckman Coulter Life Sciences
      6
      OCT 27, 2021 | 10:00 AM
      A CRISPR powered NGS library prep to improve viral, bacterial and host transcript detection in shotgun metagenomic sequencing
      A CRISPR powered NGS library prep to improve viral, bacterial...
      Date: October 27, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The COVID-19 pandemic has brought global awareness to the dangers of emerging pathogens to human health and welfare. Common molecular...
      Speaker: Jon Armstrong, MSc
      Sponsored By: Jumpcode Genomics
      6 2
      OCT 26, 2021 | 11:00 AM
      Constructing a Spatially Resolved Single-cell Atlas of the Mouse Retina with the MERSCOPE Platform
      Constructing a Spatially Resolved Single-cell Atlas of the Mouse...
      Date: October 26, 2021 Time: 11:00am (PDT), 1:00pm (EDT) The retina is the light-sensing part of the visual system and is composed of six neuron types and several non-neuronal cell types, wi...
      Speaker: Rui Chen, B.S., Ph.D.
      Sponsored By: Vizgen
      5
      SEP 28, 2021 | 9:00 AM
      New method for standardized, high-throughput PBMC isolation from blood samples for observational and clinical studies
      New method for standardized, high-throughput PBMC isolation from...
      Date: September 28, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Standardization and reproducibility are prerequisites for peripheral blood mononuclear cell (PBMC) generation from donor or patient...
      Speaker: Lotta Raty , Dr. Steffen Rapp , PhD
      Sponsored By: Miltenyi Biotec
      10 1
      JUN 02, 2021 | 7:00 AM
      At the edge of genomics: Sequencing single viral particles, One at a time.
      At the edge of genomics: Sequencing single viral particles, One...
      DATE: June 02, 2021 TIME: 7:00am PDT In this talk we will present how we have optimized single-cell technologies to sort single viral particles by advanced flow cytometry and whole genome am...
      Speaker: Dr. Manuel Martinez-Garcia
      Sponsored By: GenScript
      17 1
      MAY 18, 2021 | 7:00 AM
      Hitting the Achilles' Heel of Cancers with Confidence - Robust Validation of Neoantigens
      Hitting the Achilles' Heel of Cancers with Confidence - Robust...
      Date: May 18, 2021 Time: 7:00am PDT, 10:00am EDT Proteins encoded by mutant genes in cancers can be processed and presented on tumor cell surface by human leukocyte antigen (HLA) molecules,...
      Speaker: Qing Wang, PhD, MHS, MS
      Sponsored By: Agilent
      5 2
      APR 20, 2021 | 12:00 PM
      High Viability Cell Sorting for Single-Cell 3' RNA-seq & 10x Genomics Applications
      High Viability Cell Sorting for Single-Cell 3' RNA-seq & 10x Genomics...
      NanoCellect will discuss the importance of cell sample preparation in order to obtain good quality sequencing data with 3’ RNA-Seq as well as cell sample preparation in order to obtain...
      Speaker: Nicole Jagnandan, PhD
      Presented at: Genetics Week Virtual Event Series 2021
      Sponsored By: NanoCellect Biomedical, Inc.
      1
      APR 20, 2021 | 12:00 AM
      Cell type-specific vulnerability to traumatic brain injury
      Cell type-specific vulnerability to traumatic brain injury
      Traumatic brain injury (TBI) is best characterized as brain dysfunction caused by an outside force, usually a violent blow to the head, often occurring as a result of a severe sports injury...
      Speaker: Araks Martirosyan, Ph.D.
      Presented at: Genetics Week Virtual Event Series 2021
      1
      APR 20, 2021 | 12:00 AM
      Studying Promoter and Isoform Diversity with Long-Read Transcriptome Sequencing
      Studying Promoter and Isoform Diversity with Long-Read Transcriptome...
      Learning Objectives: 1. Learn about alternative promoters and their role in cancer 2. Learn how long read RNA-Seq helps to study the transcriptome...
      Speaker: Jonathan Göke
      Presented at: Genetics Week Virtual Event Series 2021
      1
      APR 20, 2021 | 12:00 AM
      Integrative Analysis of Single-Cell Combinatorial Long-Read RNA-seq and Single-Cell ATAC-seq in Myogenesis
      Integrative Analysis of Single-Cell Combinatorial Long-Read RNA-seq...
      Learning Objectives: 1. Demonstrate the utility of long-read RNA-sequencing in studying alternative isoforms 2. Define the long-Split-seq approach to profiling the transcriptomes of single-c...
      Speaker: Fairlie Reese, PhD Student
      Presented at: Genetics Week Virtual Event Series 2021
      1
      MAR 18, 2021 | 8:00 AM
      Facilitating transcriptomics of sensitive target cells with gentle cell sorting
      Facilitating transcriptomics of sensitive target cells with gentle...
      DATE: March 18, 2021 TIME: 8:00am PDT Sequencing of bulk cells, single cells, and nuclei is opening doors in the understanding of complex biological processes....
      Speaker: Alexander Jonsson , Dr. Alina Steinbach
      Sponsored By: Miltenyi Biotec
      11
      NOV 16, 2020 | 8:00 AM
      Targeted single-cell RNA sequencing enables genome-scale CRISPR screens
      Targeted single-cell RNA sequencing enables genome-scale CRISPR...
      Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
      Speaker: Wieland Keilholz, Ph.D. , Pawel Zajac, Ph.D. , Dr. Tilmann Buerckstuemmer
      Sponsored By: Illumina
      11
      OCT 29, 2020 | 8:00 AM
      Cell types to targets: Single cell RNA-sequencing for drug discovery
      Cell types to targets: Single cell RNA-sequencing for drug discovery
      Date: October 29, 2020 Time: 8:00am (PDT), 11:00am (EDT) What cell types cause and perpetuate disease? For many diseases, scientists only have guesses to this critical question. Single cell...
      Speaker: Sarah Middleton, PhD
      Sponsored By: 10x Genomics
      10 2
      OCT 08, 2020 | 12:00 AM
      Transcriptome analysis of breast cancer clinical specimens with long-read sequencer
      Transcriptome analysis of breast cancer clinical specimens with...
      Changes in transcriptional regulation are thought to be one of the key drivers of carcinogenesis. Although next-generation sequencer revolutionized transcriptome analysis, there are limitati...
      Speaker: Masahito Kawazu, MD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
      1
      SEP 30, 2020 | 1:30 PM
      Harnessing novel CRISPR systems for genome engineering
      Harnessing novel CRISPR systems for genome engineering
      RNA plays important and diverse roles in biology, but molecular tools to manipulate and measure RNA are limited. We demonstrate that RNA-targeting CRISPR effector Cas13 can be engineered for...
      Speaker: Omar Abudayyeh, PhD , Jonathan Gootenberg, PhD
      Presented at: CRISPR Virtual Event Series 2020
      4
      SEP 30, 2020 | 12:00 PM
      Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA
      Efficient Generation of Gene-edited Mouse Models and Cell Lines...
      The CRISPR/Cas genome editing system has revolutionized nearly every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA,...
      Speaker: Gurpreet Balrey, PhD , Peter Romanienko, PhD
      Presented at: CRISPR Virtual Event Series 2020
      Sponsored By: MilliporeSigma
      4
    MAR 28, 2024 | 8:00 AM
    Identification of Frequent Autoreactive B-cell Response in Pancreatic Ductal Adenocarcinoma Using Cell Sorting and Single-Cell Sequencing
    Identification of Frequent Autoreactive B-cell Response in Pancreatic...
    B cell responses within pancreatic ductal adenocarcinoma (PDAC) tumors are associated with improved patient outcomes, yet the specific triggering antigens remain largely unknown. In this web...
    Speaker: Min Yao, Ph.D. , Patrick Murphy, Ph.D.
    Sponsored By: Sony Biotechnology
    12
    MAR 26, 2024 | 11:00 AM
    Complete and Comprehensive Biomarker Discovery: TerraFlow for Flow Cytometry Analysis
    Complete and Comprehensive Biomarker Discovery: TerraFlow for...
    Ever wonder what you’re missing in your data? The sheer complexity of today’s flow and mass cytometry datasets demands automated solutions. Machine learning plugins only provide...
    Speaker: Dan Freeman , Pratip K. Chattopadhyay
    Sponsored By: terraFlow Bio
    19
    NOV 14, 2023 | 8:00 AM
    High-throughput RNA-Seq of clinical samples using Covaris AFA ultrasonication
    High-throughput RNA-Seq of clinical samples using Covaris AFA...
    RNA-Seq remains a critical and robust methodology in understanding cellular and organismal changes associated with development and disease. Advances in automation have helped reduce the cost...
    Speaker: Madan Ambavaram, Ph.D. , Stuart Levine, Ph.D.
    Sponsored By: Covaris
    12
    March 23/24 2023
    Advancing the Fight Against Tuberculosis (TB) with Next Generation Sequencing
    Advancing the Fight Against Tuberculosis (TB) with Next Generation...
    Date: 24 March 2023...
    Speaker: Professor Vitali Sintchenko , Dr Timothy Rodwell , Dr Melanie Grobbelaar
    Sponsored By: Illumina
    65
    FEB 14, 2023 | 10:00 AM
    A spatially-resolved transcriptional atlas of the murine dorsal pontine tegmentum
    A spatially-resolved transcriptional atlas of the murine dorsal...
    Date: February 14, 2023 Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CET) The dorsal pontine tegmentum (PnTg) is part of the Pons, the largest section of the brainstem. It is a complex region...
    Speaker: Stefano Nardone, PhD , Cory Brannigan
    Sponsored By: Vizgen
    6
     
     
  • DEC 13, 2022 | 8:00 AM
    Assessing CRISPR On-Target Editing and Structural Changes with UDiTaSâ„¢ using Tagifyâ„¢ Reagents
    Assessing CRISPR On-Target Editing and Structural Changes with...
    Date: December 13, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeu...
    Speaker: Jack Leonard, PhD , Georgia Giannoukos, PhD
    Sponsored By: seqWell
    13
    APR 19, 2022 | 1:30 PM
    C.E. CREDITS
    Boosting Signal-to-Noise in Single Cell RNA-Seq Through Molecular Depletion
    Boosting Signal-to-Noise in Single Cell RNA-Seq Through Molecular...
    Regardless of method, single cell RNA-seq only captures a small fraction of the transcriptome of each cell. Often, this is due to inherent limitations of the methodology as reads ‘drop...
    Speaker: Dante DeAscanis
    Presented at: Genetics Week Virtual Event Series 2022
    Sponsored By: Jumpcode Genomics
    3
    MAR 02, 2022 | 9:00 AM
    Double transcriptomic reads in single cell RNA-seq using molecular depletion
    Double transcriptomic reads in single cell RNA-seq using molecular...
    Date: March 02, 2022 Time: 9:00am (PST), 12:00pm (EST) Single cell RNA-seq is known to only capture a small fraction of the transcriptome of each cell. Often, this is due to inherent limitat...
    Speaker: Jon Bezney
    Sponsored By: Jumpcode Genomics
    9
    NOV 15, 2021 | 6:00 AM
    Fully automated RNA library preparation for sequencing respiratory viruses
    Fully automated RNA library preparation for sequencing respiratory...
    Date: November 15, 2021 Time: 6:00am (PDT), 9:00am (EDT) Targeted sequencing of respiratory viruses’ genomes is important to identify novel viral variants. Compared to amplicon sequenc...
    Speaker: Dr. Tomasz Zemojtel , Randy Pares
    Sponsored By: Beckman Coulter Life Sciences
    6
    OCT 27, 2021 | 10:00 AM
    A CRISPR powered NGS library prep to improve viral, bacterial and host transcript detection in shotgun metagenomic sequencing
    A CRISPR powered NGS library prep to improve viral, bacterial...
    Date: October 27, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The COVID-19 pandemic has brought global awareness to the dangers of emerging pathogens to human health and welfare. Common molecular...
    Speaker: Jon Armstrong, MSc
    Sponsored By: Jumpcode Genomics
    6 2
     
     
  • OCT 26, 2021 | 11:00 AM
    Constructing a Spatially Resolved Single-cell Atlas of the Mouse Retina with the MERSCOPE Platform
    Constructing a Spatially Resolved Single-cell Atlas of the Mouse...
    Date: October 26, 2021 Time: 11:00am (PDT), 1:00pm (EDT) The retina is the light-sensing part of the visual system and is composed of six neuron types and several non-neuronal cell types, wi...
    Speaker: Rui Chen, B.S., Ph.D.
    Sponsored By: Vizgen
    5
    SEP 28, 2021 | 9:00 AM
    New method for standardized, high-throughput PBMC isolation from blood samples for observational and clinical studies
    New method for standardized, high-throughput PBMC isolation from...
    Date: September 28, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Standardization and reproducibility are prerequisites for peripheral blood mononuclear cell (PBMC) generation from donor or patient...
    Speaker: Lotta Raty , Dr. Steffen Rapp , PhD
    Sponsored By: Miltenyi Biotec
    10 1
    JUN 02, 2021 | 7:00 AM
    At the edge of genomics: Sequencing single viral particles, One at a time.
    At the edge of genomics: Sequencing single viral particles, One...
    DATE: June 02, 2021 TIME: 7:00am PDT In this talk we will present how we have optimized single-cell technologies to sort single viral particles by advanced flow cytometry and whole genome am...
    Speaker: Dr. Manuel Martinez-Garcia
    Sponsored By: GenScript
    17 1
    MAY 18, 2021 | 7:00 AM
    Hitting the Achilles' Heel of Cancers with Confidence - Robust Validation of Neoantigens
    Hitting the Achilles' Heel of Cancers with Confidence - Robust...
    Date: May 18, 2021 Time: 7:00am PDT, 10:00am EDT Proteins encoded by mutant genes in cancers can be processed and presented on tumor cell surface by human leukocyte antigen (HLA) molecules,...
    Speaker: Qing Wang, PhD, MHS, MS
    Sponsored By: Agilent
    5 2
    APR 20, 2021 | 12:00 PM
    High Viability Cell Sorting for Single-Cell 3' RNA-seq & 10x Genomics Applications
    High Viability Cell Sorting for Single-Cell 3' RNA-seq & 10x Genomics...
    NanoCellect will discuss the importance of cell sample preparation in order to obtain good quality sequencing data with 3’ RNA-Seq as well as cell sample preparation in order to obtain...
    Speaker: Nicole Jagnandan, PhD
    Presented at: Genetics Week Virtual Event Series 2021
    Sponsored By: NanoCellect Biomedical, Inc.
    1
     
     
  • APR 20, 2021 | 12:00 AM
    Cell type-specific vulnerability to traumatic brain injury
    Cell type-specific vulnerability to traumatic brain injury
    Traumatic brain injury (TBI) is best characterized as brain dysfunction caused by an outside force, usually a violent blow to the head, often occurring as a result of a severe sports injury...
    Speaker: Araks Martirosyan, Ph.D.
    Presented at: Genetics Week Virtual Event Series 2021
    1
    APR 20, 2021 | 12:00 AM
    Studying Promoter and Isoform Diversity with Long-Read Transcriptome Sequencing
    Studying Promoter and Isoform Diversity with Long-Read Transcriptome...
    Learning Objectives: 1. Learn about alternative promoters and their role in cancer 2. Learn how long read RNA-Seq helps to study the transcriptome...
    Speaker: Jonathan Göke
    Presented at: Genetics Week Virtual Event Series 2021
    1
    APR 20, 2021 | 12:00 AM
    Integrative Analysis of Single-Cell Combinatorial Long-Read RNA-seq and Single-Cell ATAC-seq in Myogenesis
    Integrative Analysis of Single-Cell Combinatorial Long-Read RNA-seq...
    Learning Objectives: 1. Demonstrate the utility of long-read RNA-sequencing in studying alternative isoforms 2. Define the long-Split-seq approach to profiling the transcriptomes of single-c...
    Speaker: Fairlie Reese, PhD Student
    Presented at: Genetics Week Virtual Event Series 2021
    1
    MAR 18, 2021 | 8:00 AM
    Facilitating transcriptomics of sensitive target cells with gentle cell sorting
    Facilitating transcriptomics of sensitive target cells with gentle...
    DATE: March 18, 2021 TIME: 8:00am PDT Sequencing of bulk cells, single cells, and nuclei is opening doors in the understanding of complex biological processes....
    Speaker: Alexander Jonsson , Dr. Alina Steinbach
    Sponsored By: Miltenyi Biotec
    11
    NOV 16, 2020 | 8:00 AM
    Targeted single-cell RNA sequencing enables genome-scale CRISPR screens
    Targeted single-cell RNA sequencing enables genome-scale CRISPR...
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    Speaker: Wieland Keilholz, Ph.D. , Pawel Zajac, Ph.D. , Dr. Tilmann Buerckstuemmer
    Sponsored By: Illumina
    11
     
     
  • OCT 29, 2020 | 8:00 AM
    Cell types to targets: Single cell RNA-sequencing for drug discovery
    Cell types to targets: Single cell RNA-sequencing for drug discovery
    Date: October 29, 2020 Time: 8:00am (PDT), 11:00am (EDT) What cell types cause and perpetuate disease? For many diseases, scientists only have guesses to this critical question. Single cell...
    Speaker: Sarah Middleton, PhD
    Sponsored By: 10x Genomics
    10 2
    OCT 08, 2020 | 12:00 AM
    Transcriptome analysis of breast cancer clinical specimens with long-read sequencer
    Transcriptome analysis of breast cancer clinical specimens with...
    Changes in transcriptional regulation are thought to be one of the key drivers of carcinogenesis. Although next-generation sequencer revolutionized transcriptome analysis, there are limitati...
    Speaker: Masahito Kawazu, MD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
    1
    SEP 30, 2020 | 1:30 PM
    Harnessing novel CRISPR systems for genome engineering
    Harnessing novel CRISPR systems for genome engineering
    RNA plays important and diverse roles in biology, but molecular tools to manipulate and measure RNA are limited. We demonstrate that RNA-targeting CRISPR effector Cas13 can be engineered for...
    Speaker: Omar Abudayyeh, PhD , Jonathan Gootenberg, PhD
    Presented at: CRISPR Virtual Event Series 2020
    4
    SEP 30, 2020 | 12:00 PM
    Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA
    Efficient Generation of Gene-edited Mouse Models and Cell Lines...
    The CRISPR/Cas genome editing system has revolutionized nearly every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA,...
    Speaker: Gurpreet Balrey, PhD , Peter Romanienko, PhD
    Presented at: CRISPR Virtual Event Series 2020
    Sponsored By: MilliporeSigma
    4
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