Transfection
Transfection commonly refers to the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. Classically, the term transfection was used to denote the uptake of viral nucleic acid from a prokaryote‑infecting virus or bacteriophage, resulting in an infection and the production of mature virus particles. However, the term has acquired its present meaning to include any artificial introduction of foreign nucleic acid into a cell.
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MAY 16, 2019 04:00 PM CESTAdd to Calendar Select one of the following:
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MAY 16, 2019 04:00 PM CESTAdd to Calendar Select one of the following:
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DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...Speaker: Rita Colwell, PhD , Christiane Honisch, PhDSponsored By: EppendorfJUN 05, 2019 05:00 PM CESTAdd to Calendar Select one of the following:
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JUN 05, 2019 05:00 PM CESTAdd to Calendar Select one of the following:
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DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...Speaker: Jessica Wagener, PhDSponsored By: EppendorfNOV 21, 2019 09:00 AM PSTAdd to Calendar Select one of the following:
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NOV 21, 2019 09:00 AM PSTAdd to Calendar Select one of the following:
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DATE: November 21, 2019TIME: 9:00am PST, 12:00pm EST Multiple Myeloma is a disease of terminally differentiated plasma cells with the massive production of monoclonal immunoglobu...Speaker: Nathalie Hönatsch, M.Sc.Sponsored By: Thermo Fisher Scientific/GibcoAPR 17, 2019 09:00 AM PDTC.E. CREDITS
APR 17, 2019 09:00 AM PDTDATE: April 17, 2019TIME: 9:00am PDT...Speaker: Takanori TakebeSponsored By: Thermo Fisher ScientificAPR 03, 2019 09:00 AM PDTC.E. CREDITS
APR 03, 2019 09:00 AM PDTDATE: April 3, 2019TIME: 9:00am PDT...Speaker: Chris LangsdorfSponsored By: Thermo Fisher ScientificMAR 20, 2019 09:00 AM PDTC.E. CREDITS
MAR 20, 2019 09:00 AM PDTDATE: March 20, 2019TIME: 9:00am PDT...Speaker: Mark Kennedy, Ph.D.Sponsored By: Thermo Fisher ScientificFEB 20, 2019 09:00 AM PSTC.E. CREDITS
FEB 20, 2019 09:00 AM PSTDATE: February 20, 2019TIME: 9:00am PST ...Speaker: David KuningerSponsored By: Thermo Fisher ScientificNOV 15, 2018 01:00 PM PST
NOV 15, 2018 01:00 PM PSTThe Neon transfection system enables robust transfection efficiencies in hiPSC for DNA, mRNA and Cas9/gRNA RNP complexes. For hiPSC these improved transfection as well as post-electroporation...Speaker: Jackie WebbPresented At:6th annual 24 Hours of Stem Cells™ virtual eventOCT 03, 2018 12:00 PM PDTC.E. CREDITS
OCT 03, 2018 12:00 PM PDTAll forms of life require immune systems to stave off infection from viruses and other pathogens. In bacteria and archaea, clustered regularly interspaced short palindromic repeats (CRISPR) a...Speaker: Dipali Sashital, PhDPresented At:CRISPR 2018OCT 03, 2018 09:00 AM PDTC.E. CREDITS
OCT 03, 2018 09:00 AM PDTThe most recently developed genome editing system, CRISPR-Cas9 has greater inherent flexibility than prior programmable nuclease platforms. Because of its simplicity and efficacy, this techno...Speaker: Lihua Julie Zhu, PhDPresented At:CRISPR 2018SEP 27, 2018 07:30 AM PDTC.E. CREDITS
SEP 27, 2018 07:30 AM PDTCellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To uncover the underlying organizational principle...Speaker: Bo Huang, PhDPresented At:Cell Biology 2018SEP 26, 2018 12:00 PM PDTC.E. CREDITS
SEP 26, 2018 12:00 PM PDTWith the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...Speaker: Michael Johnson, PhDPresented At:Cell Biology 2018SEP 18, 2018 08:00 AM PDTC.E. CREDITS
SEP 18, 2018 08:00 AM PDTDATE: September 18,2018TIME: 08:00am PDT, 11:00am EDT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the genomes of diverse cell types and sp...Speaker: Judd Hultquist, PhDSponsored By: Horizon DiscoveryMAY 17, 2018 01:00 PM HKT
MAY 17, 2018 01:00 PM HKTHEK293和CHO细胞是快速、规模可调的悬浮培养系统中常用的细胞系，可用于瞬时蛋白表达。此次演讲介绍了瞬时表达的基本概念和使用材料，以及使用293或CHO细胞时需要考虑的主要差异。...Speaker: Jian LiuPresented At:Gibco ExpressionWorld 2018MAY 17, 2018 11:00 AM HKT
MAY 17, 2018 11:00 AM HKTExpiCHO是一种高效的瞬时表达系统，可以在2周内获得与稳定细胞株相当的表达量。 CHO细胞长期以来一直在工业界广泛使用，获得CHO表达蛋白必须构建稳定的细胞株，这一过程不仅耗时耗力，而且需要经验丰富的研发人员。在药物开发阶段需要在较短时间获得大量蛋白，往往只能采用293细胞的瞬转表达系统，但该系统产量低，表达蛋白与CHO系统存在质量差异。ExpiCHO作为高效的瞬时表达系统能...Speaker: Yuedan KongPresented At:Gibco ExpressionWorld 2018MAY 17, 2018 10:00 AM HKT
MAY 17, 2018 10:00 AM HKTGibco™ ExpiCHO 和Expi293系统是两款全新优化的瞬时重组蛋白高表达系统，目前已广泛应用于治疗性候选药物的早期鉴定，诊断性试剂的快速生产，以及疫苗研发、结构生物学和膜蛋白生物学等研究领域，成为可以在瞬转中获得极高的蛋白产量的一站式有效途径和工具。虽然多种重组蛋白均可在这两款瞬时表达系统中获得高水平表达，但HEK293 细胞和 CHO 细胞之间存在的内在...Speaker: Chao Yan LiuPresented At:Gibco ExpressionWorld 2018MAR 27, 2018 09:00 AM PDTC.E. CREDITS
MAR 27, 2018 09:00 AM PDTDATE: March 27, 2018TIME: 09:00am PDT, 12:00pm EDTThe rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, a...Speaker: Amanda HaasSponsored By: Horizon DiscoveryOCT 19, 2017 08:00 AM PDTC.E. CREDITS
OCT 19, 2017 08:00 AM PDTDATE: October 19, 2017TIME: 08:00am PDT, 11:00am EDT Recent FDA approval of the first Chimeric Antigen Receptor T cell (CAR-T) therapy offers cancer patients more promise than...Speaker: Xavier de Mollerat du JeuSponsored By: Thermo Fisher Scientific/Gibco
Recent FDA approval of the first Chimeric Antigen Receptor T cell (CAR-T) therapy offers cancer patients more promise than ever for curative effects. However, many technical challenges in T c...Speaker: Xavier de Mollerat du JeuPresented At:5th annual 24 Hours of Stem Cells™ virtual eventSEP 28, 2017 01:30 PM PDTC.E. CREDITS
SEP 28, 2017 01:30 PM PDTThe Resource Identification Initiative supports NIH's new guidelines for Rigor and Transparency as these apply in biomedical publications. Authors are instructed to authenticate key biolo...Speaker: Anita Bandrowski, PhDPresented At:Cell Biology 2017SEP 28, 2017 12:00 PM PDTC.E. CREDITS
SEP 28, 2017 12:00 PM PDTHow are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: &b...Speaker: Abhishek SharmaPresented At:Cell Biology 2017SEP 28, 2017 10:30 AM PDTC.E. CREDITS
SEP 28, 2017 10:30 AM PDTStem cells can self-renew and differentiate into multiple different cell types. Engineering of stem cells has enabled new methods to study development and organogenesis in humans as well as d...Speaker: Amr Abdeen, PhDPresented At:Cell Biology 2017SEP 28, 2017 09:00 AM PDTC.E. CREDITS
SEP 28, 2017 09:00 AM PDTContractile non-muscle cells, including smooth muscle and myoepithelial cells, provide the mechanical forces required for tissue homeostasis in numerous organ systems. For example, smooth mus...Speaker: Alison Wirshing, PhDPresented At:Cell Biology 2017SEP 28, 2017 07:30 AM PDTC.E. CREDITS
SEP 28, 2017 07:30 AM PDTI will present my lab’s effort on studying and manipulating RNA processing, with particular emphasis on using CRISPR/Cas systems for targeting RNA in living cells in diseases such as my...Speaker: Gene Yeo, PhDPresented At:Cell Biology 2017
MAY 16, 2019
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MAY 16, 2019
04:00 PM CEST
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DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
Speaker:
Rita Colwell, PhD
,
Christiane Honisch, PhD
Sponsored By: Eppendorf
JUN 05, 2019
05:00 PM CEST
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JUN 05, 2019
05:00 PM CEST
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DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
Speaker:
Jessica Wagener, PhD
Sponsored By: Eppendorf
NOV 21, 2019
09:00 AM PST
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NOV 21, 2019
09:00 AM PST
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DATE: November 21, 2019TIME: 9:00am PST, 12:00pm EST Multiple Myeloma is a disease of terminally differentiated plasma cells with the massive production of monoclonal immunoglobu...
Speaker:
Nathalie Hönatsch, M.Sc.
Sponsored By: Thermo Fisher Scientific/Gibco
APR 17, 2019
09:00 AM PDT
C.E. CREDITS
APR 17, 2019
09:00 AM PDT
DATE: April 17, 2019TIME: 9:00am PDT...
Speaker:
Takanori Takebe
Sponsored By: Thermo Fisher Scientific
APR 03, 2019
09:00 AM PDT
C.E. CREDITS
APR 03, 2019
09:00 AM PDT
DATE: April 3, 2019TIME: 9:00am PDT...
Speaker:
Chris Langsdorf
Sponsored By: Thermo Fisher Scientific
MAR 20, 2019
09:00 AM PDT
C.E. CREDITS
MAR 20, 2019
09:00 AM PDT
DATE: March 20, 2019TIME: 9:00am PDT...
Speaker:
Mark Kennedy, Ph.D.
Sponsored By: Thermo Fisher Scientific
FEB 20, 2019
09:00 AM PST
C.E. CREDITS
FEB 20, 2019
09:00 AM PST
DATE: February 20, 2019TIME: 9:00am PST ...
Speaker:
David Kuninger
Sponsored By: Thermo Fisher Scientific
NOV 15, 2018
01:00 PM PST
NOV 15, 2018
01:00 PM PST
The Neon transfection system enables robust transfection efficiencies in hiPSC for DNA, mRNA and Cas9/gRNA RNP complexes. For hiPSC these improved transfection as well as post-electroporation...
Speaker:
Jackie Webb
Presented At:6th annual 24 Hours of Stem Cells™ virtual event
OCT 03, 2018
12:00 PM PDT
C.E. CREDITS
OCT 03, 2018
12:00 PM PDT
All forms of life require immune systems to stave off infection from viruses and other pathogens. In bacteria and archaea, clustered regularly interspaced short palindromic repeats (CRISPR) a...
Speaker:
Dipali Sashital, PhD
Presented At:CRISPR 2018
OCT 03, 2018
09:00 AM PDT
C.E. CREDITS
OCT 03, 2018
09:00 AM PDT
The most recently developed genome editing system, CRISPR-Cas9 has greater inherent flexibility than prior programmable nuclease platforms. Because of its simplicity and efficacy, this techno...
Speaker:
Lihua Julie Zhu, PhD
Presented At:CRISPR 2018
SEP 27, 2018
07:30 AM PDT
C.E. CREDITS
SEP 27, 2018
07:30 AM PDT
Cellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To uncover the underlying organizational principle...
Speaker:
Bo Huang, PhD
Presented At:Cell Biology 2018
SEP 26, 2018
12:00 PM PDT
C.E. CREDITS
SEP 26, 2018
12:00 PM PDT
With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
Speaker:
Michael Johnson, PhD
Presented At:Cell Biology 2018
SEP 18, 2018
08:00 AM PDT
C.E. CREDITS
SEP 18, 2018
08:00 AM PDT
DATE: September 18,2018TIME: 08:00am PDT, 11:00am EDT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the genomes of diverse cell types and sp...
Speaker:
Judd Hultquist, PhD
Sponsored By: Horizon Discovery
MAY 17, 2018
01:00 PM HKT
MAY 17, 2018
01:00 PM HKT
HEK293和CHO细胞是快速、规模可调的悬浮培养系统中常用的细胞系，可用于瞬时蛋白表达。此次演讲介绍了瞬时表达的基本概念和使用材料，以及使用293或CHO细胞时需要考虑的主要差异。...
Speaker:
Jian Liu
Presented At:Gibco ExpressionWorld 2018
MAY 17, 2018
11:00 AM HKT
MAY 17, 2018
11:00 AM HKT
ExpiCHO是一种高效的瞬时表达系统，可以在2周内获得与稳定细胞株相当的表达量。 CHO细胞长期以来一直在工业界广泛使用，获得CHO表达蛋白必须构建稳定的细胞株，这一过程不仅耗时耗力，而且需要经验丰富的研发人员。在药物开发阶段需要在较短时间获得大量蛋白，往往只能采用293细胞的瞬转表达系统，但该系统产量低，表达蛋白与CHO系统存在质量差异。ExpiCHO作为高效的瞬时表达系统能...
Speaker:
Yuedan Kong
Presented At:Gibco ExpressionWorld 2018
MAY 17, 2018
10:00 AM HKT
MAY 17, 2018
10:00 AM HKT
Gibco™ ExpiCHO 和Expi293系统是两款全新优化的瞬时重组蛋白高表达系统，目前已广泛应用于治疗性候选药物的早期鉴定，诊断性试剂的快速生产，以及疫苗研发、结构生物学和膜蛋白生物学等研究领域，成为可以在瞬转中获得极高的蛋白产量的一站式有效途径和工具。虽然多种重组蛋白均可在这两款瞬时表达系统中获得高水平表达，但HEK293 细胞和 CHO 细胞之间存在的内在...
Speaker:
Chao Yan Liu
Presented At:Gibco ExpressionWorld 2018
MAR 27, 2018
09:00 AM PDT
C.E. CREDITS
MAR 27, 2018
09:00 AM PDT
DATE: March 27, 2018TIME: 09:00am PDT, 12:00pm EDTThe rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, a...
Speaker:
Amanda Haas
Sponsored By: Horizon Discovery
OCT 19, 2017
08:00 AM PDT
C.E. CREDITS
OCT 19, 2017
08:00 AM PDT
DATE: October 19, 2017TIME: 08:00am PDT, 11:00am EDT Recent FDA approval of the first Chimeric Antigen Receptor T cell (CAR-T) therapy offers cancer patients more promise than...
Speaker:
Xavier de Mollerat du Jeu
Sponsored By: Thermo Fisher Scientific/Gibco
Recent FDA approval of the first Chimeric Antigen Receptor T cell (CAR-T) therapy offers cancer patients more promise than ever for curative effects. However, many technical challenges in T c...
Speaker:
Xavier de Mollerat du Jeu
Presented At:5th annual 24 Hours of Stem Cells™ virtual event
SEP 28, 2017
01:30 PM PDT
C.E. CREDITS
SEP 28, 2017
01:30 PM PDT
The Resource Identification Initiative supports NIH's new guidelines for Rigor and Transparency as these apply in biomedical publications. Authors are instructed to authenticate key biolo...
Speaker:
Anita Bandrowski, PhD
Presented At:Cell Biology 2017
SEP 28, 2017
12:00 PM PDT
C.E. CREDITS
SEP 28, 2017
12:00 PM PDT
How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: &b...
Speaker:
Abhishek Sharma
Presented At:Cell Biology 2017
SEP 28, 2017
10:30 AM PDT
C.E. CREDITS
SEP 28, 2017
10:30 AM PDT
Stem cells can self-renew and differentiate into multiple different cell types. Engineering of stem cells has enabled new methods to study development and organogenesis in humans as well as d...
Speaker:
Amr Abdeen, PhD
Presented At:Cell Biology 2017
SEP 28, 2017
09:00 AM PDT
C.E. CREDITS
SEP 28, 2017
09:00 AM PDT
Contractile non-muscle cells, including smooth muscle and myoepithelial cells, provide the mechanical forces required for tissue homeostasis in numerous organ systems. For example, smooth mus...
Speaker:
Alison Wirshing, PhD
Presented At:Cell Biology 2017
SEP 28, 2017
07:30 AM PDT
C.E. CREDITS
SEP 28, 2017
07:30 AM PDT
I will present my lab’s effort on studying and manipulating RNA processing, with particular emphasis on using CRISPR/Cas systems for targeting RNA in living cells in diseases such as my...
Speaker:
Gene Yeo, PhD
Presented At:Cell Biology 2017