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    Transfection

    Transfection commonly refers to the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. Classically, the term transfection was used to denote the uptake of viral nucleic acid from a prokaryote‑infecting virus or bacteriophage, resulting in an infection and the production of mature virus particles. However, the term has acquired its present meaning to include any artificial introduction of foreign nucleic acid into a cell.

    Webinars

    • OCT 28, 2025 | 9:00 AM
      Avectas' Solupore® Transfected Cells Isolated Using Sony's CGX10 Yield Highly Cytotoxic CAR-T Cells
      Avectas' Solupore® Transfected Cells Isolated Using Sony's CGX10...
      This webinar explains how CAR-T cells transfected using Solupore® technology from Avectas and sorted using the CGX10 Cell Isolation System from Sony Biotechnology outperform unsorted cel...
      Speaker: Claudia Krause, PhD , Lisa O'Flynn, PhD
      Sponsored By: Sony Biotechnology
      5
      OCT 15, 2025 | 5:30 AM
      Non-viral, large-scale delivery of gene editing tools for T-cell manufacturing
      Non-viral, large-scale delivery of gene editing tools for T-cell...
      In recent years, non-viral methods for cell engineering have emerged as promising alternatives to viral transduction. Amongst the virus-free technologies, electroporation is considered the g...
      Speaker: Andrea Toell, PhD , Stefanie Muethel, PhD
      Presented at: Cell & Gene Therapy Virtual Event Series 2025
      Sponsored By: LONZA
      2
      OCT 08, 2025 | 9:00 AM
      Mastering Cell Counting: From Fundamentals to Advanced Techniques for Improved Accuracy
      Mastering Cell Counting: From Fundamentals to Advanced Techniques...
      Cell counting is a fundamental step in many workflows, including cell culture, cell analysis, transfection, single cell sequencing, and cell therapy. Automated cell counters have improved th...
      Speaker: Kristi Hamilton, PhD
      Sponsored By: Thermo Fisher Scientific
      14
      APR 08, 2025 | 8:00 AM
      Transfection Potency of Lipid Nanoparticles Containing mRNA Depends on Relative Loading Levels
      Transfection Potency of Lipid Nanoparticles Containing mRNA Depends...
      When formulating mRNA into lipid nanoparticles (LNP), various copy numbers of mRNA are encapsulated, leading to a distribution of mRNA loading levels within the LNPs. It is unclear whether t...
      Speaker: Dr. Suiyang Liao
      Sponsored By: Beckman Coulter Life Sciences
      13 1
      MAR 20, 2025 | 8:00 AM
      BalanCD HEK293 Viral Feed: Accelerating AAV and LV Production for Gene and Cell Therapy
      BalanCD HEK293 Viral Feed: Accelerating AAV and LV Production...
      Efficient viral vector production is essential for the cost-effective manufacture of cell and gene therapies. Many of the current production workflows are reliant on basal media in batch-mod...
      Speaker: Shan Gao, PhD
      Sponsored By: FUJIFILM Irvine Scientific
      14
      NOV 06, 2024 | 10:00 AM
      Unleashing the Power of Automation for High Throughput Antibody Synthesis
      Unleashing the Power of Automation for High Throughput Antibody...
      The discovery and optimization of antibodies, whether through traditional methods or with the assistance of artificial intelligence, necessitates rapid and reliable data generation. Here we...
      Speaker: Dr. Rebecca Moschall
      Sponsored By: Thermo Fisher Scientific
      9
      OCT 30, 2024 | 1:00 PM
      Overcome the limitations of conventional transfection with MaxCyte electroporation
      Overcome the limitations of conventional transfection with MaxCyte...
      Are low gene editing efficiency or gene expression levels preventing your research from advancing? Is your transfection method killing your cells? Are you struggling to reproduce your initia...
      Speaker: Peter Gee, Ph.D.
      Sponsored By: MaxCyte®
      5
      On Demand
      C.E. CREDITS
      Optimizing Nucleic Acid Extraction: Accelerating Progress and Ensuring Quality in Cell and Gene Therapy Development
      Optimizing Nucleic Acid Extraction: Accelerating Progress and...
      The development of cell and gene therapies faces immense pressure in terms of time and cost. Advanced therapy medicinal products (ATMPs) demand stringent quality control measures to ensure s...
      Speaker: Thomas Winkelmüller, PhD
      Presented at: Cell & Gene Therapy Virtual Event Series 2024
      4
      On Demand
      C.E. CREDITS
      Utilizing Digital Microfluidics to Miniaturize and Automate Arrayed CRISPR Screening Workflows
      Utilizing Digital Microfluidics to Miniaturize and Automate Arrayed...
      Learning Objevtives: 1. Demonstrate Knowledge with gene editing, microfluidics and non-viral delivery methods. 2. Review the uses of a low-volume high-throughput transfection platform for ge...
      Speaker: Hugo Sinha, MASc
      Presented at: Laboratory Automation Virtual Event Series 2024
      2
      FEB 22, 2023 | 9:00 AM
      A scalable miniature circular GenCircleâ„¢ dsDNA production system for gene and cell therapy
      A scalable miniature circular GenCircleâ„¢ dsDNA production system...
      Date: February 22, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) Circular plasmid DNA has traditionally been used as a stable and efficient vector for gene expression and HDR-mediated...
      Speaker: Dr. Fan Zhou, PhD
      Sponsored By: GenScript
      7
      FEB 02, 2023 | 11:00 AM
      Break the Bottleneck: Automate Plasmid Maxi-prep with AmMagâ„¢ Quatro
      Break the Bottleneck: Automate Plasmid Maxi-prep with AmMagâ„¢ Quatro
      Date: December 13, 2022 Time: 11:00am (PST), 2:00pm (EST), 8:00pm (CEST) Plasmid DNA (pDNA) is an essential component of molecular biotechnology applications, such as protein expression and...
      Speaker: Rouba Najjar, MBA
      Sponsored By: GenScript
      11 1
      OCT 12, 2022 | 8:30 PM
      Tilting against windmills: Clarity in spectral panel design without losing the battle for biological resolution
      Tilting against windmills: Clarity in spectral panel design without...
      Date: October 12, 2022, October 13, 2022 Time: 8:30pm (PDT), 11:30pm (EDT), 5:30am (CEST) High dimensional full spectrum flow cytometry holds the promise of addressing long-held questions in...
      Speaker: Seddon Thomas
      Sponsored By: Thermo Fisher Scientific
      12
      JUL 26, 2022 | 9:00 AM
      Applications in Cancer Research: RNA Isolation from Organoids and Spheroids
      Applications in Cancer Research: RNA Isolation from Organoids...
      Date: July 26, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Organoids and spheroids are increasingly being used in many research applications, including drug discovery, toxicology,...
      Speaker: Laura Chapman , Anupriya Gupta , Jay Bhandari
      Sponsored By: Thermo Fisher Scientific
      24
      APR 21, 2022 | 5:30 AM
      Hypoxia Biomarkers: Personalizing Cancer Treatments by Targeting Hypoxia
      Hypoxia Biomarkers: Personalizing Cancer Treatments by Targeting...
      A hypoxic tumour microenvironment is a feature of solid tumours. Many tumour types contain high fractions of hypoxic tissue. The relationship between high levels of tumour hypoxia and a poor...
      Speaker: Joely Irlam
      Presented at: Genetics Week Virtual Event Series 2022
      2
      OCT 14, 2021 | 9:00 AM
      Session 3: Simple steps to publication-quality western blots
      Session 3: Simple steps to publication-quality western blots
      Date: October 14, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe...
      Speaker: Kelli Feather-Henigan
      Presented at: Webinar series: Simple steps to publication-quality western blots
      Sponsored By: Thermo Fisher Scientific
      28
      OCT 13, 2021 | 7:00 AM
      Session 2: Simple steps to publication-quality western blots
      Session 2: Simple steps to publication-quality western blots
      Date: October 13, 2021 Time: 7:00am (PDT), 10:00am (EDT) Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target...
      Speaker: Caleb Shearrow
      Presented at: Webinar series: Simple steps to publication-quality western blots
      Sponsored By: Thermo Fisher Scientific
      31 1
      OCT 12, 2021 | 5:00 PM
      Session 1: Simple steps to publication-quality western blots
      Session 1: Simple steps to publication-quality western blots
      Date: October 12, 2021 Time: 5:00pm (PDT), 8:00pm (EDT) 222 Learning Objectives 222 222 222 Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved...
      Speaker: Jordan Thompson, PhD
      Presented at: Webinar series: Simple steps to publication-quality western blots
      Sponsored By: Thermo Fisher Scientific
      39 1
      OCT 06, 2021 | 9:00 AM
      Antibody titration for flow cytometric analysis of Extracellular Vesicles: Are cell based protocols up to the task?
      Antibody titration for flow cytometric analysis of Extracellular...
      Date: October 6, 2021 Time: 9:00am (PDT), 12:00pm (EDT) ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis...
      Speaker: Desmond Pink, PhD
      Sponsored By: Miltenyi Biotec
      6 1
      SEP 14, 2021 | 7:00 AM
      What prevents you from increasing your PCR productivity?
      What prevents you from increasing your PCR productivity?
      Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
      Speaker: Arora Phang, PhD
      Sponsored By: Eppendorf
      24 1
      AUG 31, 2021 | 9:00 AM
      The Regulation of ALC1 by TRIM33 during the DNA Damage Response
      The Regulation of ALC1 by TRIM33 during the DNA Damage Response
      Date: July 07, 2021 Time: 8:00am (PDT), 11:00am (EDT) The DNA damage response is extremely crucial in maintaining genomic integrity. Failure to repair damaged DNA can result in the propagati...
      Speaker: Rukia Henry
      Sponsored By: Thermo Fisher Scientific/Gibco
      4
      JUL 27, 2021 | 8:00 AM
      Scaling-Up Exosomes and Other Extracellular Vesicles
      Scaling-Up Exosomes and Other Extracellular Vesicles
      Date: July 27, 2021 Time: 8:00am (PDT), 11:00am (EDT) The Origin of Biopharmaceutics (Biopharma) derive from The Division of Clinical Research in the U.S. Bureau of Medicine. However, author...
      Speaker: Dr. Derek Lenz
      Sponsored By: Beckman Coulter Life Sciences
      16 3
      JUL 01, 2021 | 12:00 PM
      Mastering basic cell culture techniques
      Mastering basic cell culture techniques
      Date: July 01, 2021 Time: 09:30am IST/12:00pm SGT/01:00pm JST, KST/03:00pm AEST/ 05:00pm NZST We know that understanding cell culture is essential for life of your work. Gibco Galaxy Tour We...
      Speaker: Brittany Balhouse , Christopher Scanlon
      Sponsored By: Thermo Fisher Scientific/Gibco
      48 3
      JUN 09, 2021 | 7:00 AM
      Preventing cell misidentification in your daily lab routine
      Preventing cell misidentification in your daily lab routine
      Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
      Speaker: Gabriela Saldanha , Jessica Wagener, PhD
      Sponsored By: Eppendorf
      40 3
      JUN 08, 2021 | 9:00 AM
      Innovations in Vaccine Research and Development
      Innovations in Vaccine Research and Development
      Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
      Speaker: Laura Chapman , Ketil Pedersen, Ph.D , Alexander "Sasha" Vlassov , Matt Mckenna
      Sponsored By: Thermo Fisher Scientific
      28 4
    OCT 28, 2025 | 9:00 AM
    Avectas' Solupore® Transfected Cells Isolated Using Sony's CGX10 Yield Highly Cytotoxic CAR-T Cells
    Avectas' Solupore® Transfected Cells Isolated Using Sony's CGX10...
    This webinar explains how CAR-T cells transfected using Solupore® technology from Avectas and sorted using the CGX10 Cell Isolation System from Sony Biotechnology outperform unsorted cel...
    Speaker: Claudia Krause, PhD , Lisa O'Flynn, PhD
    Sponsored By: Sony Biotechnology
    5
    OCT 15, 2025 | 5:30 AM
    Non-viral, large-scale delivery of gene editing tools for T-cell manufacturing
    Non-viral, large-scale delivery of gene editing tools for T-cell...
    In recent years, non-viral methods for cell engineering have emerged as promising alternatives to viral transduction. Amongst the virus-free technologies, electroporation is considered the g...
    Speaker: Andrea Toell, PhD , Stefanie Muethel, PhD
    Presented at: Cell & Gene Therapy Virtual Event Series 2025
    Sponsored By: LONZA
    2
    OCT 08, 2025 | 9:00 AM
    Mastering Cell Counting: From Fundamentals to Advanced Techniques for Improved Accuracy
    Mastering Cell Counting: From Fundamentals to Advanced Techniques...
    Cell counting is a fundamental step in many workflows, including cell culture, cell analysis, transfection, single cell sequencing, and cell therapy. Automated cell counters have improved th...
    Speaker: Kristi Hamilton, PhD
    Sponsored By: Thermo Fisher Scientific
    14
    APR 08, 2025 | 8:00 AM
    Transfection Potency of Lipid Nanoparticles Containing mRNA Depends on Relative Loading Levels
    Transfection Potency of Lipid Nanoparticles Containing mRNA Depends...
    When formulating mRNA into lipid nanoparticles (LNP), various copy numbers of mRNA are encapsulated, leading to a distribution of mRNA loading levels within the LNPs. It is unclear whether t...
    Speaker: Dr. Suiyang Liao
    Sponsored By: Beckman Coulter Life Sciences
    13 1
    MAR 20, 2025 | 8:00 AM
    BalanCD HEK293 Viral Feed: Accelerating AAV and LV Production for Gene and Cell Therapy
    BalanCD HEK293 Viral Feed: Accelerating AAV and LV Production...
    Efficient viral vector production is essential for the cost-effective manufacture of cell and gene therapies. Many of the current production workflows are reliant on basal media in batch-mod...
    Speaker: Shan Gao, PhD
    Sponsored By: FUJIFILM Irvine Scientific
    14
     
     
  • NOV 06, 2024 | 10:00 AM
    Unleashing the Power of Automation for High Throughput Antibody Synthesis
    Unleashing the Power of Automation for High Throughput Antibody...
    The discovery and optimization of antibodies, whether through traditional methods or with the assistance of artificial intelligence, necessitates rapid and reliable data generation. Here we...
    Speaker: Dr. Rebecca Moschall
    Sponsored By: Thermo Fisher Scientific
    9
    OCT 30, 2024 | 1:00 PM
    Overcome the limitations of conventional transfection with MaxCyte electroporation
    Overcome the limitations of conventional transfection with MaxCyte...
    Are low gene editing efficiency or gene expression levels preventing your research from advancing? Is your transfection method killing your cells? Are you struggling to reproduce your initia...
    Speaker: Peter Gee, Ph.D.
    Sponsored By: MaxCyte®
    5
    On Demand
    C.E. CREDITS
    Optimizing Nucleic Acid Extraction: Accelerating Progress and Ensuring Quality in Cell and Gene Therapy Development
    Optimizing Nucleic Acid Extraction: Accelerating Progress and...
    The development of cell and gene therapies faces immense pressure in terms of time and cost. Advanced therapy medicinal products (ATMPs) demand stringent quality control measures to ensure s...
    Speaker: Thomas Winkelmüller, PhD
    Presented at: Cell & Gene Therapy Virtual Event Series 2024
    4
    On Demand
    C.E. CREDITS
    Utilizing Digital Microfluidics to Miniaturize and Automate Arrayed CRISPR Screening Workflows
    Utilizing Digital Microfluidics to Miniaturize and Automate Arrayed...
    Learning Objevtives: 1. Demonstrate Knowledge with gene editing, microfluidics and non-viral delivery methods. 2. Review the uses of a low-volume high-throughput transfection platform for ge...
    Speaker: Hugo Sinha, MASc
    Presented at: Laboratory Automation Virtual Event Series 2024
    2
    FEB 22, 2023 | 9:00 AM
    A scalable miniature circular GenCircleâ„¢ dsDNA production system for gene and cell therapy
    A scalable miniature circular GenCircleâ„¢ dsDNA production system...
    Date: February 22, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) Circular plasmid DNA has traditionally been used as a stable and efficient vector for gene expression and HDR-mediated...
    Speaker: Dr. Fan Zhou, PhD
    Sponsored By: GenScript
    7
     
     
  • FEB 02, 2023 | 11:00 AM
    Break the Bottleneck: Automate Plasmid Maxi-prep with AmMagâ„¢ Quatro
    Break the Bottleneck: Automate Plasmid Maxi-prep with AmMagâ„¢ Quatro
    Date: December 13, 2022 Time: 11:00am (PST), 2:00pm (EST), 8:00pm (CEST) Plasmid DNA (pDNA) is an essential component of molecular biotechnology applications, such as protein expression and...
    Speaker: Rouba Najjar, MBA
    Sponsored By: GenScript
    11 1
    OCT 12, 2022 | 8:30 PM
    Tilting against windmills: Clarity in spectral panel design without losing the battle for biological resolution
    Tilting against windmills: Clarity in spectral panel design without...
    Date: October 12, 2022, October 13, 2022 Time: 8:30pm (PDT), 11:30pm (EDT), 5:30am (CEST) High dimensional full spectrum flow cytometry holds the promise of addressing long-held questions in...
    Speaker: Seddon Thomas
    Sponsored By: Thermo Fisher Scientific
    12
    JUL 26, 2022 | 9:00 AM
    Applications in Cancer Research: RNA Isolation from Organoids and Spheroids
    Applications in Cancer Research: RNA Isolation from Organoids...
    Date: July 26, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Organoids and spheroids are increasingly being used in many research applications, including drug discovery, toxicology,...
    Speaker: Laura Chapman , Anupriya Gupta , Jay Bhandari
    Sponsored By: Thermo Fisher Scientific
    24
    APR 21, 2022 | 5:30 AM
    Hypoxia Biomarkers: Personalizing Cancer Treatments by Targeting Hypoxia
    Hypoxia Biomarkers: Personalizing Cancer Treatments by Targeting...
    A hypoxic tumour microenvironment is a feature of solid tumours. Many tumour types contain high fractions of hypoxic tissue. The relationship between high levels of tumour hypoxia and a poor...
    Speaker: Joely Irlam
    Presented at: Genetics Week Virtual Event Series 2022
    2
    OCT 14, 2021 | 9:00 AM
    Session 3: Simple steps to publication-quality western blots
    Session 3: Simple steps to publication-quality western blots
    Date: October 14, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe...
    Speaker: Kelli Feather-Henigan
    Presented at: Webinar series: Simple steps to publication-quality western blots
    Sponsored By: Thermo Fisher Scientific
    28
     
     
  • OCT 13, 2021 | 7:00 AM
    Session 2: Simple steps to publication-quality western blots
    Session 2: Simple steps to publication-quality western blots
    Date: October 13, 2021 Time: 7:00am (PDT), 10:00am (EDT) Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target...
    Speaker: Caleb Shearrow
    Presented at: Webinar series: Simple steps to publication-quality western blots
    Sponsored By: Thermo Fisher Scientific
    31 1
    OCT 12, 2021 | 5:00 PM
    Session 1: Simple steps to publication-quality western blots
    Session 1: Simple steps to publication-quality western blots
    Date: October 12, 2021 Time: 5:00pm (PDT), 8:00pm (EDT) 222 Learning Objectives 222 222 222 Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved...
    Speaker: Jordan Thompson, PhD
    Presented at: Webinar series: Simple steps to publication-quality western blots
    Sponsored By: Thermo Fisher Scientific
    39 1
    OCT 06, 2021 | 9:00 AM
    Antibody titration for flow cytometric analysis of Extracellular Vesicles: Are cell based protocols up to the task?
    Antibody titration for flow cytometric analysis of Extracellular...
    Date: October 6, 2021 Time: 9:00am (PDT), 12:00pm (EDT) ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis...
    Speaker: Desmond Pink, PhD
    Sponsored By: Miltenyi Biotec
    6 1
    SEP 14, 2021 | 7:00 AM
    What prevents you from increasing your PCR productivity?
    What prevents you from increasing your PCR productivity?
    Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
    Speaker: Arora Phang, PhD
    Sponsored By: Eppendorf
    24 1
    AUG 31, 2021 | 9:00 AM
    The Regulation of ALC1 by TRIM33 during the DNA Damage Response
    The Regulation of ALC1 by TRIM33 during the DNA Damage Response
    Date: July 07, 2021 Time: 8:00am (PDT), 11:00am (EDT) The DNA damage response is extremely crucial in maintaining genomic integrity. Failure to repair damaged DNA can result in the propagati...
    Speaker: Rukia Henry
    Sponsored By: Thermo Fisher Scientific/Gibco
    4
     
     
  • JUL 27, 2021 | 8:00 AM
    Scaling-Up Exosomes and Other Extracellular Vesicles
    Scaling-Up Exosomes and Other Extracellular Vesicles
    Date: July 27, 2021 Time: 8:00am (PDT), 11:00am (EDT) The Origin of Biopharmaceutics (Biopharma) derive from The Division of Clinical Research in the U.S. Bureau of Medicine. However, author...
    Speaker: Dr. Derek Lenz
    Sponsored By: Beckman Coulter Life Sciences
    16 3
    JUL 01, 2021 | 12:00 PM
    Mastering basic cell culture techniques
    Mastering basic cell culture techniques
    Date: July 01, 2021 Time: 09:30am IST/12:00pm SGT/01:00pm JST, KST/03:00pm AEST/ 05:00pm NZST We know that understanding cell culture is essential for life of your work. Gibco Galaxy Tour We...
    Speaker: Brittany Balhouse , Christopher Scanlon
    Sponsored By: Thermo Fisher Scientific/Gibco
    48 3
    JUN 09, 2021 | 7:00 AM
    Preventing cell misidentification in your daily lab routine
    Preventing cell misidentification in your daily lab routine
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    Speaker: Gabriela Saldanha , Jessica Wagener, PhD
    Sponsored By: Eppendorf
    40 3
    JUN 08, 2021 | 9:00 AM
    Innovations in Vaccine Research and Development
    Innovations in Vaccine Research and Development
    Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
    Speaker: Laura Chapman , Ketil Pedersen, Ph.D , Alexander "Sasha" Vlassov , Matt Mckenna
    Sponsored By: Thermo Fisher Scientific
    28 4
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