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    Transfection

    Transfection commonly refers to the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. Classically, the term transfection was used to denote the uptake of viral nucleic acid from a prokaryote‑infecting virus or bacteriophage, resulting in an infection and the production of mature virus particles. However, the term has acquired its present meaning to include any artificial introduction of foreign nucleic acid into a cell.

    Webinars

    • AUG 25, 2020 | 8:00 AM
      How LV and AAV Transfection Platforms will Shape the Future of Gene Therapy Manufacturing
      How LV and AAV Transfection Platforms will Shape the Future of...
      DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
      Speaker: Leisha Kopp
      Sponsored By: Mirus
      18 2
      JUN 30, 2020 | 9:00 AM
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
      DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
      Speaker: Jane Weitzel
      Sponsored By: Sartorius
      17 1
      JUN 18, 2020 | 9:00 AM
      Unveiling the role of T cell dysfunction in the development and progression of glioblastoma
      Unveiling the role of T cell dysfunction in the development and...
      DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...
      Speaker: Felipe Galvez Cancino, Ph.D.
      Sponsored By: Thermo Fisher Scientific/Gibco
      18 2
      JUN 02, 2020 | 7:00 AM
      Harnessing the Power of Protein Expression Systems in Vaccine Development
      Harnessing the Power of Protein Expression Systems in Vaccine...
      DATE: June 2, 2020 TIME: 7:00am PT, 10:00am ET In just a few months COVID-19 has risen from a regional crisis to a global threat, and drug makers are now scrambling to develop vaccines aimed...
      Speaker: Roland Leathers, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      15 1
      MAY 12, 2020 | 9:00 AM
      Analytical Quality Control for the Pharmaceutical Industry
      Analytical Quality Control for the Pharmaceutical Industry
      NEW DATE: Date: May 12, 2020 Time: 9:00am PDT, 12:00pm EDT...
      Speaker: Joy McElroy
      Sponsored By: Sartorius
      29 9
      APR 30, 2020 | 9:00 AM
      Tools and methods for CRISPR-Cas9 screening for functional genomics and drug discovery
      Tools and methods for CRISPR-Cas9 screening for functional genomics...
      DATE: April 30, 2020 TIME: 9:00am PT, 12:00pm ET Loss-of-function studies are an established method for uncovering a gene’s role in a biological pathway or disease state. The advent of...
      Speaker: Louise Baskin, M.S. Molecular Biology and Genetics
      Sponsored By: Thermo Fisher Scientific
      23 1
      APR 28, 2020 | 9:00 AM
      ExpiSf Expression System: A Chemically Defined Baculovirus-Based Expression System for Enhanced Protein and Virus Production in Sf9 Cells
      ExpiSf Expression System: A Chemically Defined Baculovirus-Based...
      DATE: April 28, 2020 TIME: 9:00am PT, 12:00pm ET The Baculovirus Expression Vector System is a versatile platform for expression of individual recombinant proteins, membrane proteins, virus-...
      Speaker: Kenneth Thompson, Ph.D.
      Sponsored By: Thermo Fisher Scientific/Gibco
      4
      APR 14, 2020 | 9:00 AM
      Gibco Cell Culture Heroes: Work From Home Edition
      Gibco Cell Culture Heroes: Work From Home Edition
      DATE: April 14, 2020 TIME: 9:00am PDT, 12:00pm EDT Besides being Gibco Cell Culture Heroes, what do Vivek, Sandra, Daisy, Ameet and Kristine all have in common? They are all stuck at home fo...
      Speaker: Kristine Wadosky, PhD , Ameet Chimote, PhD , Elizabeth Delery, PhD , Daisy Shu, B.Optom, PhD , Vivek Kamat, PhD , Sandra Hammer, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      12 1
      MAR 31, 2020 | 7:00 AM
      Investigation of NK cell-driven tumor immunity using novel image cytometry detection method
      Investigation of NK cell-driven tumor immunity using novel image...
      DATE: March 31, 2020 TIME: 7:00am PT, 10:00am PT Please join us for this webinar, during which we will present and discuss the promotion of NK cell-driven tumor immunity. In addition, we wil...
      Speaker: Lucas Ferrari de Andrade, Ph.D. , Leo Chan, Ph.D.
      Sponsored By: Nexcelom Bioscience
      10
      MAR 27, 2020 | 9:00 AM
      Modified Dental Composite for Bone Repair
      Modified Dental Composite for Bone Repair
      DATE: March 27, 2020 TIME: 9:00am PDT, 12:00pm EDT Osteoporosis causes a decrease in bone density, along with deterioration of the bone’s microarchitecture at a faster rate than normal...
      Speaker: Mayda Arshad
      Sponsored By: Thermo Fisher Scientific/Gibco
      11
      MAR 26, 2020 | 9:00 AM
      Cutting Edge Viral and Non-Viral Delivery Platforms for T-Cell Engineering and Beyond
      Cutting Edge Viral and Non-Viral Delivery Platforms for T-Cell...
      DATE: March 26, 2020 TIME: 9:00am PST, 12:00pm EST Growing demand for precision therapy and the recent successes with CAR-T cells for cancer treatment...
      Speaker: Namritha Ravinder
      Sponsored By: Thermo Fisher Scientific
      7
      JAN 30, 2020 | 9:00 AM
      An Automated Cancer Organoid Culture Platform to Accelerate Research and Drug Screening
      An Automated Cancer Organoid Culture Platform to Accelerate Research...
      DATE: January 30, 2020 TIME: 9:00am PST, 12:00pm EST Recent advances in in vitro 3D cellular culture technologies, such as organoids, rapidly developed and established novel, more physiologi...
      Speaker: Brooke Schuster
      Sponsored By: Thermo Fisher Scientific/Gibco
      10
      JAN 23, 2020 | 9:00 AM
      A Workflow for Knock-in Genome Editing: Simplified
      A Workflow for Knock-in Genome Editing: Simplified
      DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
      Speaker: Matthew Poling, PhD
      Sponsored By: Thermo Fisher Scientific
      17
      DEC 17, 2019 | 9:00 AM
      The role of SIRT1/LXR signaling in diabetic retinopathy
      The role of SIRT1/LXR signaling in diabetic retinopathy
      DATE: December 17, 2019TIME: 9:00am PST, 12:00pm EST Purpose: Liver x receptors (LXRs) are hypothesized to serve as a link between lipid metabolism and inflammation by prom...
      Speaker: Sandra Hammer, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      6
      DEC 04, 2019 | 7:00 AM
      Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
      Improve your reproducibility - Liquid Handling is a crucial factor...
      DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
      Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
      Sponsored By: Eppendorf
      12
      NOV 21, 2019 | 9:00 AM
      The role of immune cells and immune checkpoints in multiple myeloma
      The role of immune cells and immune checkpoints in multiple myeloma
      DATE: November 21, 2019TIME: 9:00am PST, 12:00pm EST Multiple Myeloma is a disease of terminally differentiated plasma cells with the massive production of monoclonal immunoglobu...
      Speaker: Nathalie Heider-Hönatsch, M.Sc.
      Sponsored By: Thermo Fisher Scientific/Gibco
      11
      NOV 18, 2019 | 7:00 AM
      Protect your cells with proper pipetting - How liquid handling influences your cell culture work
      Protect your cells with proper pipetting - How liquid handling...
      DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
      Speaker: Nadine Mellies, PhD , Katja Baesler, PhD
      Sponsored By: Eppendorf
      22 2
      NOV 07, 2019 | 9:00 AM
      Factor V Expression in ExpiCHO Cells
      Factor V Expression in ExpiCHO Cells
      DATE: November 7, 2019 TIME: 9:00am PST, 12:00pm EST In collaboration with Hematherix and Thermo Fisher, Dr. Laurent Mosnier is investigating a new treatment for acute traumatic bl...
      Speaker: Laurent Mosnier, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      5 2
      NOV 05, 2019 | 9:00 AM
      Minicircle DNA: a new material for CAR-T and AAV vector particles - its development and analysis
      Minicircle DNA: a new material for CAR-T and AAV vector particles...
      DATE: November 5, 2019TIME: 9:00am PT, 12:00pm ET New results have highlighted the advantages of minicircles over plasmids as they avoid unnecessary bacterial backbone sequences...
      Speaker: Martin Schleef, PhD
      Sponsored By: SCIEX
      4
      OCT 31, 2019 | 9:00 AM
      Investigation of Endothelial Cell Viability and Growth on 3D printed GelMa Vascular Networks
      Investigation of Endothelial Cell Viability and Growth on 3D printed...
      DATE: October 31, 2019TIME: 9:00am PDT, 12:00pm EDT INTRODUCTION: A major limitation for the development of 3D engineered tissues is the absence of viable and perfusable...
      Speaker: Chrisoula Chappell
      Sponsored By: Thermo Fisher Scientific/Gibco
      8
      SEP 25, 2019 | 8:00 AM
      Expression, Purification and Characterization of Difficult to Express Membrane Proteins in the Expi293 and ExpiCHO Expression Systems:  Case study of Potassium chloride co-transporter KCC2
      Expression, Purification and Characterization of Difficult to...
      DATE: September 25, 2019TIME: 8:00am PT, 11:00am ET This talk will focus on strategies for optimizing expression levels of membrane proteins using the Gibco™ Expi293™ and ExpiCHO™ Expres...
      Speaker: Anass Jawhari, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      4
      SEP 05, 2019 | 7:00 AM
      The bread and butter method of genomics: How to improve your (q)PCR
      The bread and butter method of genomics: How to improve your (q)PCR
      DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
      Speaker: Tim Schommartz, PhD
      Sponsored By: Eppendorf
      17 1
      AUG 29, 2019 | 9:00 AM
      Enabling Next-Generation Targeted siRNA Therapies with ExpiCHO
      Enabling Next-Generation Targeted siRNA Therapies with ExpiCHO
      DATE: August 29, 2019TIME: 9:00am PT/ 12:00pm ET Monoclonal antibodies (mAbs) are a cornerstone of modern biomedical research and biotherapeutics. Therefore, reproducibly obtai...
      Speaker: Ian Huggins, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      6
      JUN 19, 2019 | 10:00 AM
      Getting started in 3D:  Ask an Expert
      Getting started in 3D: Ask an Expert
      DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
      Speaker: Mark Kennedy, Ph.D. , Richard Josephson, PhD
      Sponsored By: Thermo Fisher Scientific
      6
    AUG 25, 2020 | 8:00 AM
    How LV and AAV Transfection Platforms will Shape the Future of Gene Therapy Manufacturing
    How LV and AAV Transfection Platforms will Shape the Future of...
    DATE: August 25, 2020 TIME: 8:00am PDT, 10:00am CDT, 11:00am EDT Recombinant lentivirus (LV) and adeno-associated virus (AAV) are critical components of cell and gene therapies, which show g...
    Speaker: Leisha Kopp
    Sponsored By: Mirus
    18 2
    JUN 30, 2020 | 9:00 AM
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
    DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
    Speaker: Jane Weitzel
    Sponsored By: Sartorius
    17 1
    JUN 18, 2020 | 9:00 AM
    Unveiling the role of T cell dysfunction in the development and progression of glioblastoma
    Unveiling the role of T cell dysfunction in the development and...
    DATE: June 18, 2020 TIME: 9:00am PDT, 12:00pm EDT Despite the success of immunotherapy against several malignancies including melanoma and lung adenocarcinoma, glioblastoma remains the excep...
    Speaker: Felipe Galvez Cancino, Ph.D.
    Sponsored By: Thermo Fisher Scientific/Gibco
    18 2
    JUN 02, 2020 | 7:00 AM
    Harnessing the Power of Protein Expression Systems in Vaccine Development
    Harnessing the Power of Protein Expression Systems in Vaccine...
    DATE: June 2, 2020 TIME: 7:00am PT, 10:00am ET In just a few months COVID-19 has risen from a regional crisis to a global threat, and drug makers are now scrambling to develop vaccines aimed...
    Speaker: Roland Leathers, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    15 1
    MAY 12, 2020 | 9:00 AM
    Analytical Quality Control for the Pharmaceutical Industry
    Analytical Quality Control for the Pharmaceutical Industry
    NEW DATE: Date: May 12, 2020 Time: 9:00am PDT, 12:00pm EDT...
    Speaker: Joy McElroy
    Sponsored By: Sartorius
    29 9
     
     
  • APR 30, 2020 | 9:00 AM
    Tools and methods for CRISPR-Cas9 screening for functional genomics and drug discovery
    Tools and methods for CRISPR-Cas9 screening for functional genomics...
    DATE: April 30, 2020 TIME: 9:00am PT, 12:00pm ET Loss-of-function studies are an established method for uncovering a gene’s role in a biological pathway or disease state. The advent of...
    Speaker: Louise Baskin, M.S. Molecular Biology and Genetics
    Sponsored By: Thermo Fisher Scientific
    23 1
    APR 28, 2020 | 9:00 AM
    ExpiSf Expression System: A Chemically Defined Baculovirus-Based Expression System for Enhanced Protein and Virus Production in Sf9 Cells
    ExpiSf Expression System: A Chemically Defined Baculovirus-Based...
    DATE: April 28, 2020 TIME: 9:00am PT, 12:00pm ET The Baculovirus Expression Vector System is a versatile platform for expression of individual recombinant proteins, membrane proteins, virus-...
    Speaker: Kenneth Thompson, Ph.D.
    Sponsored By: Thermo Fisher Scientific/Gibco
    4
    APR 14, 2020 | 9:00 AM
    Gibco Cell Culture Heroes: Work From Home Edition
    Gibco Cell Culture Heroes: Work From Home Edition
    DATE: April 14, 2020 TIME: 9:00am PDT, 12:00pm EDT Besides being Gibco Cell Culture Heroes, what do Vivek, Sandra, Daisy, Ameet and Kristine all have in common? They are all stuck at home fo...
    Speaker: Kristine Wadosky, PhD , Ameet Chimote, PhD , Elizabeth Delery, PhD , Daisy Shu, B.Optom, PhD , Vivek Kamat, PhD , Sandra Hammer, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    12 1
    MAR 31, 2020 | 7:00 AM
    Investigation of NK cell-driven tumor immunity using novel image cytometry detection method
    Investigation of NK cell-driven tumor immunity using novel image...
    DATE: March 31, 2020 TIME: 7:00am PT, 10:00am PT Please join us for this webinar, during which we will present and discuss the promotion of NK cell-driven tumor immunity. In addition, we wil...
    Speaker: Lucas Ferrari de Andrade, Ph.D. , Leo Chan, Ph.D.
    Sponsored By: Nexcelom Bioscience
    10
    MAR 27, 2020 | 9:00 AM
    Modified Dental Composite for Bone Repair
    Modified Dental Composite for Bone Repair
    DATE: March 27, 2020 TIME: 9:00am PDT, 12:00pm EDT Osteoporosis causes a decrease in bone density, along with deterioration of the bone’s microarchitecture at a faster rate than normal...
    Speaker: Mayda Arshad
    Sponsored By: Thermo Fisher Scientific/Gibco
    11
     
     
  • MAR 26, 2020 | 9:00 AM
    Cutting Edge Viral and Non-Viral Delivery Platforms for T-Cell Engineering and Beyond
    Cutting Edge Viral and Non-Viral Delivery Platforms for T-Cell...
    DATE: March 26, 2020 TIME: 9:00am PST, 12:00pm EST Growing demand for precision therapy and the recent successes with CAR-T cells for cancer treatment...
    Speaker: Namritha Ravinder
    Sponsored By: Thermo Fisher Scientific
    7
    JAN 30, 2020 | 9:00 AM
    An Automated Cancer Organoid Culture Platform to Accelerate Research and Drug Screening
    An Automated Cancer Organoid Culture Platform to Accelerate Research...
    DATE: January 30, 2020 TIME: 9:00am PST, 12:00pm EST Recent advances in in vitro 3D cellular culture technologies, such as organoids, rapidly developed and established novel, more physiologi...
    Speaker: Brooke Schuster
    Sponsored By: Thermo Fisher Scientific/Gibco
    10
    JAN 23, 2020 | 9:00 AM
    A Workflow for Knock-in Genome Editing: Simplified
    A Workflow for Knock-in Genome Editing: Simplified
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    Speaker: Matthew Poling, PhD
    Sponsored By: Thermo Fisher Scientific
    17
    DEC 17, 2019 | 9:00 AM
    The role of SIRT1/LXR signaling in diabetic retinopathy
    The role of SIRT1/LXR signaling in diabetic retinopathy
    DATE: December 17, 2019TIME: 9:00am PST, 12:00pm EST Purpose: Liver x receptors (LXRs) are hypothesized to serve as a link between lipid metabolism and inflammation by prom...
    Speaker: Sandra Hammer, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    6
    DEC 04, 2019 | 7:00 AM
    Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
    Improve your reproducibility - Liquid Handling is a crucial factor...
    DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
    Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
    Sponsored By: Eppendorf
    12
     
     
  • NOV 21, 2019 | 9:00 AM
    The role of immune cells and immune checkpoints in multiple myeloma
    The role of immune cells and immune checkpoints in multiple myeloma
    DATE: November 21, 2019TIME: 9:00am PST, 12:00pm EST Multiple Myeloma is a disease of terminally differentiated plasma cells with the massive production of monoclonal immunoglobu...
    Speaker: Nathalie Heider-Hönatsch, M.Sc.
    Sponsored By: Thermo Fisher Scientific/Gibco
    11
    NOV 18, 2019 | 7:00 AM
    Protect your cells with proper pipetting - How liquid handling influences your cell culture work
    Protect your cells with proper pipetting - How liquid handling...
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    Speaker: Nadine Mellies, PhD , Katja Baesler, PhD
    Sponsored By: Eppendorf
    22 2
    NOV 07, 2019 | 9:00 AM
    Factor V Expression in ExpiCHO Cells
    Factor V Expression in ExpiCHO Cells
    DATE: November 7, 2019 TIME: 9:00am PST, 12:00pm EST In collaboration with Hematherix and Thermo Fisher, Dr. Laurent Mosnier is investigating a new treatment for acute traumatic bl...
    Speaker: Laurent Mosnier, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    5 2
    NOV 05, 2019 | 9:00 AM
    Minicircle DNA: a new material for CAR-T and AAV vector particles - its development and analysis
    Minicircle DNA: a new material for CAR-T and AAV vector particles...
    DATE: November 5, 2019TIME: 9:00am PT, 12:00pm ET New results have highlighted the advantages of minicircles over plasmids as they avoid unnecessary bacterial backbone sequences...
    Speaker: Martin Schleef, PhD
    Sponsored By: SCIEX
    4
    OCT 31, 2019 | 9:00 AM
    Investigation of Endothelial Cell Viability and Growth on 3D printed GelMa Vascular Networks
    Investigation of Endothelial Cell Viability and Growth on 3D printed...
    DATE: October 31, 2019TIME: 9:00am PDT, 12:00pm EDT INTRODUCTION: A major limitation for the development of 3D engineered tissues is the absence of viable and perfusable...
    Speaker: Chrisoula Chappell
    Sponsored By: Thermo Fisher Scientific/Gibco
    8
     
     
  • SEP 25, 2019 | 8:00 AM
    Expression, Purification and Characterization of Difficult to Express Membrane Proteins in the Expi293 and ExpiCHO Expression Systems:  Case study of Potassium chloride co-transporter KCC2
    Expression, Purification and Characterization of Difficult to...
    DATE: September 25, 2019TIME: 8:00am PT, 11:00am ET This talk will focus on strategies for optimizing expression levels of membrane proteins using the Gibco™ Expi293™ and ExpiCHO™ Expres...
    Speaker: Anass Jawhari, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    4
    SEP 05, 2019 | 7:00 AM
    The bread and butter method of genomics: How to improve your (q)PCR
    The bread and butter method of genomics: How to improve your (q)PCR
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    Speaker: Tim Schommartz, PhD
    Sponsored By: Eppendorf
    17 1
    AUG 29, 2019 | 9:00 AM
    Enabling Next-Generation Targeted siRNA Therapies with ExpiCHO
    Enabling Next-Generation Targeted siRNA Therapies with ExpiCHO
    DATE: August 29, 2019TIME: 9:00am PT/ 12:00pm ET Monoclonal antibodies (mAbs) are a cornerstone of modern biomedical research and biotherapeutics. Therefore, reproducibly obtai...
    Speaker: Ian Huggins, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    6
    JUN 19, 2019 | 10:00 AM
    Getting started in 3D:  Ask an Expert
    Getting started in 3D: Ask an Expert
    DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
    Speaker: Mark Kennedy, Ph.D. , Richard Josephson, PhD
    Sponsored By: Thermo Fisher Scientific
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