MENU
JUN 20, 2019 10:30 AM PDT

Beyond the Score: Tools for Pan-Biomarker Analysis and TMB Estimation Enabling Deeper Understanding of Somatic Alterations that Contribute to Cancer Initiation, Progression and Recurrence

Sponsored by: QIAGEN
Speakers

Abstract

Tumor mutational burden (TMB) is an emerging biomarker that correlates with response to immunotherapeutic agents, such as checkpoint inhibitors. Recent studies indicate that a high mutation load increases the likelihood that immunogenic neoantigens expressed by tumor cells may induce a response to immunotherapy.  However, TMB estimation and reporting can be heavily influenced by differing working processes across clinical and research laboratories; primarily, the choice of assay, platform, and how the assay is implemented and interpreted. 

In the first half of the webinar, we introduce the QIAseq Tumor Mutational Burden (TMB) Panel, a comprehensive next-generation sequencing (NGS) assay that targets the full coding region of 486 genes implicated in the pathogenesis of solid tumors and can be boosted to add 27 microsatellite instability (MSI) markers. From optimized primer design based on the robust UMI-enabled single primer extension (SPE) chemistry, which reduces false positives and eliminates duplicate reads, and now coupled with the CLC Genomics Workbench, the QIAseq Tumor Mutation Burden Panel ensures TMB estimation consistent with best practices as reported by the Friends of Cancer Research (Genes, Chromosomes & Cancer, 2019). With QIAGEN Clinical Insight (QCI™) – Interpret, insights for variants detected, based on private and publicly available data sources, are provided, enabling a better understanding of these variants and their role in tumor initiation, growth, spread and recurrence.

In the second half we present a scalable bioinformatics solution for the interpretation of somatic mutations. Cancer laboratories need rapid and reliable interpretation of identified genomic alterations. One of the challenges they face is producing standardized, reproducible interpretation and reporting of the most current and actionable information. QIAGEN Clinical Insight (QCI™) Interpret enables labs to deliver evidence-based, actionable insights and reporting of variants and TMB and MSI scores from tumor genomic profiles. We will discuss the benefits of automating guidelines (AMP/ASCO/CAP and ACMG/AMP) for vetting somatic cancer alterations for biological and actionable relevance while providing the evidence behind each automated classification via direct links to the source, facilitating insights that improve clinical trial matching.

Learning Objectives: 

1. Learn how the coupled solution of UMI-enabled single primer extension (SPE) and the well renowned CLC algorithms for secondary analysis enables the coverage and high confidence detection of variants, even those at low frequency (as low as 0.4%) while minimizing impact of PCR duplicates and false positives which could inflate TMB scores
2. Learn how the QIAGEN Clinical Insight (QCI™)- Interpret clinical decision support platform can enable rapid and consistent reporting of variant pathogenicity and actionability, resulting in better clinical trial matching.


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUL 15, 2021 8:00 AM PDT
C.E. CREDITS
JUL 15, 2021 8:00 AM PDT
Date: July 15, 2021 Time: 8:00am (PDT), 11:00am (EDT) High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Flu...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
OCT 20, 2021 10:00 AM PDT
C.E. CREDITS
OCT 20, 2021 10:00 AM PDT
Date: October 20, 2021 Time:10:00am (PDT), 1:00pm (EDT) As the prevalence of Diabetes continues to rise in many areas across the globe, healthcare providers continue to look for methods that...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
JUN 20, 2019 10:30 AM PDT

Beyond the Score: Tools for Pan-Biomarker Analysis and TMB Estimation Enabling Deeper Understanding of Somatic Alterations that Contribute to Cancer Initiation, Progression and Recurrence

Sponsored by: QIAGEN


Show Resources
Loading Comments...
Show Resources