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    Cell

    Cell: is the smallest structural and functional unit of an organism, typically microscopic and consisting of cytoplasm and a nucleus enclosed in a membrane. Microscopic organisms typically consist of a single cell, which is either eukaryotic or prokaryotic.

    Webinars

    • APR 19, 2022 | 1:30 PM
      C.E. CREDITS
      Boosting Signal-to-Noise in Single Cell RNA-Seq Through Molecular Depletion
      Boosting Signal-to-Noise in Single Cell RNA-Seq Through Molecular...
      Regardless of method, single cell RNA-seq only captures a small fraction of the transcriptome of each cell. Often, this is due to inherent limitations of the methodology as reads ‘drop...
      Speaker: Dante DeAscanis
      Presented at: Genetics Week Virtual Event Series 2022
      Sponsored By: Jumpcode Genomics
      3
      APR 19, 2022 | 12:00 PM
      C.E. CREDITS
      RT-LAMP: Best Practices and Tips to Accelerate Detection of Viral Pathogens, Including SARS-CoV-2
      RT-LAMP: Best Practices and Tips to Accelerate Detection of Viral...
      Loop-mediated isothermal amplification (LAMP) uses a stand-displacing DNA polymerase and four to six primers to rapidly amplify DNA at a single temperature. By eliminating temperature cyclin...
      Speaker: Ingrida VendelÄ—, PhD , Leann Buhrow, PhD
      Presented at: Genetics Week Virtual Event Series 2022
      Sponsored By: Thermo Fisher Scientific
      3
      APR 19, 2022 | 10:30 AM
      Panel Presentation: Attain Successful Single-cell Sequencing Results with the Latest Cell and Nuclei Isolation Workflows
      Panel Presentation: Attain Successful Single-cell Sequencing Results...
      Revolutionary sequencing technologies are enabling whole transcriptome profiling of tens to hundreds of thousands of single cells in parallel, in a single experiment. This has led to an expl...
      Speaker: Carina Emery , Alina Steinbach
      Presented at: Genetics Week Virtual Event Series 2022
      Sponsored By: Miltenyi Biotec
      1
      APR 19, 2022 | 7:30 AM
      C.E. CREDITS
      Keynote Presentation: Ultra-Rapid Nanopore Whole Genome Sequencing in a Critical Care Setting
      Keynote Presentation: Ultra-Rapid Nanopore Whole Genome Sequencing...
      While early genetic diagnosis can guide critical care management, the turnaround time for whole genome based diagnostic testing is months. Recent programs in neonatal populations have reduce...
      Speaker: John E. Gorzynski, DVM, PhD
      Presented at: Genetics Week Virtual Event Series 2022
      2
      APR 19, 2022 | 5:30 AM
      Delivering Fast, Flexible Next-Generation Sequencing through Innovations in Chemistry and Engineering
      Delivering Fast, Flexible Next-Generation Sequencing through Innovations...
      Next-generation sequencing (NGS) has become a foundational tool for both biological research and in-vitro diagnostics, particularly in oncology, immunology, and detection of genetic disorder...
      Speaker: Timothy Looney, PhD
      Presented at: Genetics Week Virtual Event Series 2022
      3
      APR 19, 2022 | 5:30 AM
      C.E. CREDITS
      Quantitative and Spatial Differences in Host Response Dynamics to SARS-CoV-2 Variants of Concern
      Quantitative and Spatial Differences in Host Response Dynamics...
      Recurrently emerging SARS-CoV-2 variants of concern (VOC) present increasingly sophisticated immune escape strategies that fuel global infection rates, as exemplified by the raging B.1.1.529...
      Speaker: Silvia Groiss, MSc, PhD
      Presented at: Genetics Week Virtual Event Series 2022
      2
      APR 06, 2022 | 10:30 AM
      Panel Presentation: An Overview of Gene Therapy Manufacturing: Development through Commercial Production
      Panel Presentation: An Overview of Gene Therapy Manufacturing:...
      Learning Objectives: 1. Explain what is gene therapy is. 2. Explain what steps are involved for getting a gene therapy product to the clinic. 3. Discuss development and manufacturing process...
      Speaker: Kevin G. McGarry, Jr., PhD , Samir Acharya, PhD
      Presented at: Bioprocessing Virtual Event Series 2022
      3
      APR 06, 2022 | 9:00 AM
      Keynote Presentation: Continuous Bioprocessing - New Challenges and Opportunities for Membrane Technology
      Keynote Presentation: Continuous Bioprocessing - New Challenges...
      There is growing interest in the development of integrated continuous bioprocesses for the production of important biotherapeutics due to the potential for enhanced productivity, greater fle...
      Speaker: Andrew L. Zydney, PhD
      Presented at: Bioprocessing Virtual Event Series 2022
      1
      APR 06, 2022 | 5:30 AM
      Leveraging Microscale Automated Bioreactors for the Design and Optimization of Microbial Cell Factories for the Production of Anticancer Drugs Taxol
      Leveraging Microscale Automated Bioreactors for the Design and...
      Currently over 95 % of pharmaceutical products and transport fuels are derived from petroleum. Sustainable alternatives are critical to achieving net-zero emissions without detriment to qual...
      Speaker: Leonardo Rios Solis, PhD
      Presented at: Bioprocessing Virtual Event Series 2022
      2
      APR 06, 2022 | 5:30 AM
      Introduction to Next Generation Bioanalytics Development
      Introduction to Next Generation Bioanalytics Development
      Viral Vector gene therapy has been the focus of intense research driven by the safety profile and several clinical breakthroughs. As of March 2022, there are two viral vector gene therapies...
      Speaker: Nagarjun Kasaraneni, PhD
      Presented at: Bioprocessing Virtual Event Series 2022
      2
      APR 06, 2022 | 5:30 AM
      Model-Assisted Design, Transfer and Scale-Up of an Antibody-Producing Cell Culture Process
      Model-Assisted Design, Transfer and Scale-Up of an Antibody-Producing...
      Biopharmaceuticals produced with mammalian cells are the key drivers for the medication of former untreatable diseases. The development of their production processes is still challenging bec...
      Speaker: Prof. Dr.-Ing. Habil. Ralf Pörtner
      Presented at: Bioprocessing Virtual Event Series 2022
      1
      APR 06, 2022 | 5:30 AM
      Optimising Cell Line Development for Bioprocessing
      Optimising Cell Line Development for Bioprocessing
      In the last decades, the upstream processing in biopharma industry has been revolutionized regarding the production of bioactive materials such as monoclonal antibodies, proteins and biother...
      Speaker: Sami Ullah, PhD
      Presented at: Bioprocessing Virtual Event Series 2022
      5
      APR 05, 2022 | 10:00 AM
      Demystifying machine learning: driving image analysis using your biological knowledge
      Demystifying machine learning: driving image analysis using your...
      Date: April 05, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CET) Machine learning (ML) offers many benefits to researchers using a wide range of microscopy techniques. Easier workflows,...
      Speaker: Quyen Tran, Ph.D.
      Sponsored By: Leica Microsystems
      13
      APR 05, 2022 | 9:00 AM
      Enterovirus membrane-assisted assembly and release revealed by cryo-electron tomography
      Enterovirus membrane-assisted assembly and release revealed by...
      Date: April 5, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Enteroviruses are non-enveloped positive-sense RNA viruses that cause diverse diseases...
      Speaker: Selma Dahmane , Kristian Wadel
      Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
      Sponsored By: Thermo Fisher Scientific - Electron Microscopy
      7
      MAR 31, 2022 | 11:00 AM
      In situ single-cell transcriptomic imaging in formalin-fixed paraffin-embedded (FFPE) tissues with MERSCOPE
      In situ single-cell transcriptomic imaging in formalin-fixed paraffin-embedded...
      Date: March 31, 2022 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CET) Formalin-fixed paraffin-embedded (FFPE) tissues are the most widely used clinical sample types in histology and molecular...
      Speaker: Jiang He
      Sponsored By: Vizgen
      8 1
      MAR 31, 2022 | 6:00 AM
      CytoFLEX SRT: The CytoFLEX That Sorts
      CytoFLEX SRT: The CytoFLEX That Sorts
      Date: March 31, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The CytoFLEX SRT Is a compact cell sorter capable of handling the variety...
      Speaker: John Tigges
      Sponsored By: Beckman Coulter
      7
      MAR 30, 2022 | 8:00 AM
      Using Chemically Defined Media for Cell Culture
      Using Chemically Defined Media for Cell Culture
      Date: March 30, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEDT) Learn how to make the switch: Join us to discover the benefits of Chemically Defined Media conditions over serum-free an...
      Speaker: Shahram Shahabi, MD, PhD
      Sponsored By: FUJIFILM Irvine Scientific
      8
      MAR 24, 2022 | 10:00 AM
      Oncotopix Discovery: deep learning as a standard analysis method for digital pathology images
      Oncotopix Discovery: deep learning as a standard analysis method...
      Date: March 24, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CET) Easy-to-use AI-based deep learning is now integrated as the standard analysis methodology in our best-in-class analysis s...
      Speaker: Fabian Schneider, Ph.D. , David Mason, PhD
      Sponsored By: Visiopharm®
      6
      MAR 24, 2022 | 8:00 AM
      T cell separation: Fine-tuning your T cell research workflow step by step
      T cell separation: Fine-tuning your T cell research workflow step...
      Date: March 24, 2021 Time: 08:00am (PDT), 11:00pm (EDT) Simply enough, we want to treat our cells well. Afterall, we want them to tell us their stories in basic research or work for us in th...
      Speaker: Marcello Stein, PhD.
      Sponsored By: Miltenyi Biotec
      6
      MAR 23, 2022 | 11:55 PM
      Overcoming Challenges Associated with Analysis of Pesticides Regulated by Colorado State in Hemp Using a Novel LC/MS/MS Method with Dual ESI and APCI Source
      Overcoming Challenges Associated with Analysis of Pesticides Regulated...
      Like any other agricultural crop, pesticides are applied to hemp plants to protect it from pests and improve growth yield. Chronic exposure to pesticides can lead to serious health risks and...
      Speaker: Avinash Dalmia, PhD
      Presented at: Cannabis Sciences Virtual Event Series 2022
      2
      MAR 23, 2022 | 12:00 PM
      Struggling to Follow the USDA's New Hemp Testing Rules: LIMS Makes it Easy
      Struggling to Follow the USDA's New Hemp Testing Rules: LIMS Makes...
      The agricultural sector has been through significant changes since the 2018 Farm Bill was enacted. Hemp production was legalized at the federal level in the United States. Hemp production an...
      Speaker: Arun Apte
      Presented at: Cannabis Sciences Virtual Event Series 2022
      2
      MAR 22, 2022 | 7:00 AM
      Spatio-temporal characterization and quantification of DNA replication events by molecular combing
      Spatio-temporal characterization and quantification of DNA replication...
      Date: March 22, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) DNA replication happens at the very early stage of cell development, at such early stages of development...
      Speaker: Cyndie Forestier
      Sponsored By: Genomic Vision
      7
      MAR 15, 2022 | 8:00 AM
      Role of Batch Centrifugation during Cell Therapy Manufacturing
      Role of Batch Centrifugation during Cell Therapy Manufacturing
      Date: March 15, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEDT) Centrifuges are pillars of most biology applications. As cell therapy manufacturing studies have been picking up pace, t...
      Speaker: Dr. Atalay Tok
      Sponsored By: Thermo Fisher Scientific
      7
      MAR 10, 2022 | 11:55 PM
      Characterizing Pathology-Inducing Protein Aggregates as Tools for Alzheimer's and Parkinson's Disease Models and Drug Discovery
      Characterizing Pathology-Inducing Protein Aggregates as Tools...
      Alzheimer’s and Parkinson’s diseases (AD, PD) are the two most common neurodegenerative disorders. Despite their debilitating effects and increasing prevalence in our society, no...
      Speaker: Jacob McPhail, PhD
      Presented at: Neuroscience Virtual Event Series 2022
      2 1
    APR 19, 2022 | 1:30 PM
    C.E. CREDITS
    Boosting Signal-to-Noise in Single Cell RNA-Seq Through Molecular Depletion
    Boosting Signal-to-Noise in Single Cell RNA-Seq Through Molecular...
    Regardless of method, single cell RNA-seq only captures a small fraction of the transcriptome of each cell. Often, this is due to inherent limitations of the methodology as reads ‘drop...
    Speaker: Dante DeAscanis
    Presented at: Genetics Week Virtual Event Series 2022
    Sponsored By: Jumpcode Genomics
    3
    APR 19, 2022 | 12:00 PM
    C.E. CREDITS
    RT-LAMP: Best Practices and Tips to Accelerate Detection of Viral Pathogens, Including SARS-CoV-2
    RT-LAMP: Best Practices and Tips to Accelerate Detection of Viral...
    Loop-mediated isothermal amplification (LAMP) uses a stand-displacing DNA polymerase and four to six primers to rapidly amplify DNA at a single temperature. By eliminating temperature cyclin...
    Speaker: Ingrida VendelÄ—, PhD , Leann Buhrow, PhD
    Presented at: Genetics Week Virtual Event Series 2022
    Sponsored By: Thermo Fisher Scientific
    3
    APR 19, 2022 | 10:30 AM
    Panel Presentation: Attain Successful Single-cell Sequencing Results with the Latest Cell and Nuclei Isolation Workflows
    Panel Presentation: Attain Successful Single-cell Sequencing Results...
    Revolutionary sequencing technologies are enabling whole transcriptome profiling of tens to hundreds of thousands of single cells in parallel, in a single experiment. This has led to an expl...
    Speaker: Carina Emery , Alina Steinbach
    Presented at: Genetics Week Virtual Event Series 2022
    Sponsored By: Miltenyi Biotec
    1
    APR 19, 2022 | 7:30 AM
    C.E. CREDITS
    Keynote Presentation: Ultra-Rapid Nanopore Whole Genome Sequencing in a Critical Care Setting
    Keynote Presentation: Ultra-Rapid Nanopore Whole Genome Sequencing...
    While early genetic diagnosis can guide critical care management, the turnaround time for whole genome based diagnostic testing is months. Recent programs in neonatal populations have reduce...
    Speaker: John E. Gorzynski, DVM, PhD
    Presented at: Genetics Week Virtual Event Series 2022
    2
    APR 19, 2022 | 5:30 AM
    Delivering Fast, Flexible Next-Generation Sequencing through Innovations in Chemistry and Engineering
    Delivering Fast, Flexible Next-Generation Sequencing through Innovations...
    Next-generation sequencing (NGS) has become a foundational tool for both biological research and in-vitro diagnostics, particularly in oncology, immunology, and detection of genetic disorder...
    Speaker: Timothy Looney, PhD
    Presented at: Genetics Week Virtual Event Series 2022
    3
     
     
  • APR 19, 2022 | 5:30 AM
    C.E. CREDITS
    Quantitative and Spatial Differences in Host Response Dynamics to SARS-CoV-2 Variants of Concern
    Quantitative and Spatial Differences in Host Response Dynamics...
    Recurrently emerging SARS-CoV-2 variants of concern (VOC) present increasingly sophisticated immune escape strategies that fuel global infection rates, as exemplified by the raging B.1.1.529...
    Speaker: Silvia Groiss, MSc, PhD
    Presented at: Genetics Week Virtual Event Series 2022
    2
    APR 06, 2022 | 10:30 AM
    Panel Presentation: An Overview of Gene Therapy Manufacturing: Development through Commercial Production
    Panel Presentation: An Overview of Gene Therapy Manufacturing:...
    Learning Objectives: 1. Explain what is gene therapy is. 2. Explain what steps are involved for getting a gene therapy product to the clinic. 3. Discuss development and manufacturing process...
    Speaker: Kevin G. McGarry, Jr., PhD , Samir Acharya, PhD
    Presented at: Bioprocessing Virtual Event Series 2022
    3
    APR 06, 2022 | 9:00 AM
    Keynote Presentation: Continuous Bioprocessing - New Challenges and Opportunities for Membrane Technology
    Keynote Presentation: Continuous Bioprocessing - New Challenges...
    There is growing interest in the development of integrated continuous bioprocesses for the production of important biotherapeutics due to the potential for enhanced productivity, greater fle...
    Speaker: Andrew L. Zydney, PhD
    Presented at: Bioprocessing Virtual Event Series 2022
    1
    APR 06, 2022 | 5:30 AM
    Leveraging Microscale Automated Bioreactors for the Design and Optimization of Microbial Cell Factories for the Production of Anticancer Drugs Taxol
    Leveraging Microscale Automated Bioreactors for the Design and...
    Currently over 95 % of pharmaceutical products and transport fuels are derived from petroleum. Sustainable alternatives are critical to achieving net-zero emissions without detriment to qual...
    Speaker: Leonardo Rios Solis, PhD
    Presented at: Bioprocessing Virtual Event Series 2022
    2
    APR 06, 2022 | 5:30 AM
    Introduction to Next Generation Bioanalytics Development
    Introduction to Next Generation Bioanalytics Development
    Viral Vector gene therapy has been the focus of intense research driven by the safety profile and several clinical breakthroughs. As of March 2022, there are two viral vector gene therapies...
    Speaker: Nagarjun Kasaraneni, PhD
    Presented at: Bioprocessing Virtual Event Series 2022
    2
     
     
  • APR 06, 2022 | 5:30 AM
    Model-Assisted Design, Transfer and Scale-Up of an Antibody-Producing Cell Culture Process
    Model-Assisted Design, Transfer and Scale-Up of an Antibody-Producing...
    Biopharmaceuticals produced with mammalian cells are the key drivers for the medication of former untreatable diseases. The development of their production processes is still challenging bec...
    Speaker: Prof. Dr.-Ing. Habil. Ralf Pörtner
    Presented at: Bioprocessing Virtual Event Series 2022
    1
    APR 06, 2022 | 5:30 AM
    Optimising Cell Line Development for Bioprocessing
    Optimising Cell Line Development for Bioprocessing
    In the last decades, the upstream processing in biopharma industry has been revolutionized regarding the production of bioactive materials such as monoclonal antibodies, proteins and biother...
    Speaker: Sami Ullah, PhD
    Presented at: Bioprocessing Virtual Event Series 2022
    5
    APR 05, 2022 | 10:00 AM
    Demystifying machine learning: driving image analysis using your biological knowledge
    Demystifying machine learning: driving image analysis using your...
    Date: April 05, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CET) Machine learning (ML) offers many benefits to researchers using a wide range of microscopy techniques. Easier workflows,...
    Speaker: Quyen Tran, Ph.D.
    Sponsored By: Leica Microsystems
    13
    APR 05, 2022 | 9:00 AM
    Enterovirus membrane-assisted assembly and release revealed by cryo-electron tomography
    Enterovirus membrane-assisted assembly and release revealed by...
    Date: April 5, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Enteroviruses are non-enveloped positive-sense RNA viruses that cause diverse diseases...
    Speaker: Selma Dahmane , Kristian Wadel
    Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
    Sponsored By: Thermo Fisher Scientific - Electron Microscopy
    7
    MAR 31, 2022 | 11:00 AM
    In situ single-cell transcriptomic imaging in formalin-fixed paraffin-embedded (FFPE) tissues with MERSCOPE
    In situ single-cell transcriptomic imaging in formalin-fixed paraffin-embedded...
    Date: March 31, 2022 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CET) Formalin-fixed paraffin-embedded (FFPE) tissues are the most widely used clinical sample types in histology and molecular...
    Speaker: Jiang He
    Sponsored By: Vizgen
    8 1
     
     
  • MAR 31, 2022 | 6:00 AM
    CytoFLEX SRT: The CytoFLEX That Sorts
    CytoFLEX SRT: The CytoFLEX That Sorts
    Date: March 31, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The CytoFLEX SRT Is a compact cell sorter capable of handling the variety...
    Speaker: John Tigges
    Sponsored By: Beckman Coulter
    7
    MAR 30, 2022 | 8:00 AM
    Using Chemically Defined Media for Cell Culture
    Using Chemically Defined Media for Cell Culture
    Date: March 30, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEDT) Learn how to make the switch: Join us to discover the benefits of Chemically Defined Media conditions over serum-free an...
    Speaker: Shahram Shahabi, MD, PhD
    Sponsored By: FUJIFILM Irvine Scientific
    8
    MAR 24, 2022 | 10:00 AM
    Oncotopix Discovery: deep learning as a standard analysis method for digital pathology images
    Oncotopix Discovery: deep learning as a standard analysis method...
    Date: March 24, 2022 Time: 10:00am (PDT), 1:00pm (EDT), 7:00pm (CET) Easy-to-use AI-based deep learning is now integrated as the standard analysis methodology in our best-in-class analysis s...
    Speaker: Fabian Schneider, Ph.D. , David Mason, PhD
    Sponsored By: Visiopharm®
    6
    MAR 24, 2022 | 8:00 AM
    T cell separation: Fine-tuning your T cell research workflow step by step
    T cell separation: Fine-tuning your T cell research workflow step...
    Date: March 24, 2021 Time: 08:00am (PDT), 11:00pm (EDT) Simply enough, we want to treat our cells well. Afterall, we want them to tell us their stories in basic research or work for us in th...
    Speaker: Marcello Stein, PhD.
    Sponsored By: Miltenyi Biotec
    6
    MAR 23, 2022 | 11:55 PM
    Overcoming Challenges Associated with Analysis of Pesticides Regulated by Colorado State in Hemp Using a Novel LC/MS/MS Method with Dual ESI and APCI Source
    Overcoming Challenges Associated with Analysis of Pesticides Regulated...
    Like any other agricultural crop, pesticides are applied to hemp plants to protect it from pests and improve growth yield. Chronic exposure to pesticides can lead to serious health risks and...
    Speaker: Avinash Dalmia, PhD
    Presented at: Cannabis Sciences Virtual Event Series 2022
    2
     
     
  • MAR 23, 2022 | 12:00 PM
    Struggling to Follow the USDA's New Hemp Testing Rules: LIMS Makes it Easy
    Struggling to Follow the USDA's New Hemp Testing Rules: LIMS Makes...
    The agricultural sector has been through significant changes since the 2018 Farm Bill was enacted. Hemp production was legalized at the federal level in the United States. Hemp production an...
    Speaker: Arun Apte
    Presented at: Cannabis Sciences Virtual Event Series 2022
    2
    MAR 22, 2022 | 7:00 AM
    Spatio-temporal characterization and quantification of DNA replication events by molecular combing
    Spatio-temporal characterization and quantification of DNA replication...
    Date: March 22, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) DNA replication happens at the very early stage of cell development, at such early stages of development...
    Speaker: Cyndie Forestier
    Sponsored By: Genomic Vision
    7
    MAR 15, 2022 | 8:00 AM
    Role of Batch Centrifugation during Cell Therapy Manufacturing
    Role of Batch Centrifugation during Cell Therapy Manufacturing
    Date: March 15, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEDT) Centrifuges are pillars of most biology applications. As cell therapy manufacturing studies have been picking up pace, t...
    Speaker: Dr. Atalay Tok
    Sponsored By: Thermo Fisher Scientific
    7
    MAR 10, 2022 | 11:55 PM
    Characterizing Pathology-Inducing Protein Aggregates as Tools for Alzheimer's and Parkinson's Disease Models and Drug Discovery
    Characterizing Pathology-Inducing Protein Aggregates as Tools...
    Alzheimer’s and Parkinson’s diseases (AD, PD) are the two most common neurodegenerative disorders. Despite their debilitating effects and increasing prevalence in our society, no...
    Speaker: Jacob McPhail, PhD
    Presented at: Neuroscience Virtual Event Series 2022
    2 1
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