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    Clinical methods

    Clinical methods describe the techniques and analyses used to collect, process, and analyze clinical data. Clinical methods are used to reach a conclusion about a patient in a medical situation. There are various goals that can be achieved by following clinical methods, including diagnosing a disease, planning a treatment regimen, or monitoring the efficacy of a treatment

    Webinars

    • NOV 24, 2015 | 6:00 AM
      Say goodbye to Gels - Real world use of LabChip micro-fluidic technology for nucleic acid and protein analysis
      Say goodbye to Gels - Real world use of LabChip micro-fluidic...
      DATE: November 24, 2015TIME: 9am EST, 2pm GMTFor many years now, gels have been a fundamental research tool in academic research for the separation and analysis of nucleic acids and pro...
      Speaker: Julius Kostan, PhD , Sandra Doran , Veronika Delcheva
      Sponsored By: Revvity
      29
      NOV 19, 2015 | 7:00 AM
      WEBINAR: Glycated Hemoglobin (HbA1c) for Diagnosis and Monitoring of Diabetes: Current Practice
      WEBINAR: Glycated Hemoglobin (HbA1c) for Diagnosis and Monitoring...
      DATE: November 19, 2015 TIME: 7:00AM Pacific, 9:00AM Central, 10:00AM Eastern, 3:00PM GMT Diabetes represents a serious global epidemic.  Diabetes causes its own direct adverse ...
      Speaker: Cas Weykamp, PhD , Garry John, PhD
      Sponsored By: Abbott Molecular Diagnostic, Abbott Molecular Diagnostic
      10 10
      NOV 17, 2015 | 8:00 AM
      Unleashing the power of biobanks to drive translational science forward
      Unleashing the power of biobanks to drive translational science...
      DATE: November 17th, 2015TIME: 8am PDT, 11am EDT, 4pm GMTBiobanks provide important capacity to collect large genomic data sets intended to enable the study of genetic signatures of an...
      Speaker: Conny Mathay , Andrew Brooks, Ph.D. , Jens Hoefkens, PhD
      Sponsored By: Revvity
      22
      NOV 12, 2015 | 10:30 AM
      Pharmacogenetics in the Clinical Laboratory: Opportunities and Challenges
      Pharmacogenetics in the Clinical Laboratory: Opportunities and...
      Appropriate dosing of pharmaceuticals is critical to prevent sub-therapeutic efficacy or the occurrence of adverse events. However, genetic variability may significantly influence an individu...
      Speaker: Allison Chambliss, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      4
      NOV 12, 2015 | 9:00 AM
      Introducing an ancestrally diverse whole genome data set for select cohort and control research applications
      Introducing an ancestrally diverse whole genome data set for select...
      Introduction and adoption of whole-genome sequencing has enabled new methods of investigation in the quest for answers in diagnostic odyssey cases as well as in the broader study of genetic d...
      Speaker: Benjamin Solomon, MD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      3
      NOV 12, 2015 | 7:30 AM
      Keynote: Reconceptualizing Genital Herpes Diagnosis and Management
      Keynote: Reconceptualizing Genital Herpes Diagnosis and Management
      As one of the nation's most prevalent sexually transmitted infections, genital herpes represents a diagnostic and therapeutic challenge to clinicians, laboratorians and the patients they...
      Speaker: Edward Hook, III, MD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      2 1
      NOV 11, 2015 | 1:30 PM
      Why to validate diagnostic tests?
      Why to validate diagnostic tests?
      This lecture will provide attendees with the knowledge and skills to bring on new clinical laboratory tests in clinical chemistry and molecular diagnostics. The lecture will provide a general...
      Speaker: Wieslaw Furmaga, MD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      4
      NOV 11, 2015 | 10:30 AM
      Reliability of LDL cholesterol: How low can we go and when are we fooling ourselves?
      Reliability of LDL cholesterol: How low can we go and when are...
      Aggressive low-density lipoprotein cholesterol (LDL-C) lowering strategies are recommended for prevention of cardiovascular events in high-risk populations. Guidelines recommend a 30-50% redu...
      Speaker: Jeffrey Meeusen, Ph.D., DABCC
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      2
      NOV 11, 2015 | 10:30 AM
      Role of LC/MS/MS spectrometry in diagnosis of various endocrine disorders
      Role of LC/MS/MS spectrometry in diagnosis of various endocrine...
          Until recently most of the phenotypic information on congenital endocrine disorders have relied on biochemical testing of steroids, biogenic amines and peptides but is now...
      Speaker: Ravinder Singh, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      0
      NOV 11, 2015 | 10:30 AM
      Agilent StreamSelect LC/MS System: Future solutions for the high throughput lab
      Agilent StreamSelect LC/MS System: Future solutions for the high...
      Liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) is ideally suited for the direct and rapid analysis of prepared biological samples. While analysis times can be shortened...
      Speaker: Kevin McCann, BS
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      5
      NOV 11, 2015 | 7:30 AM
      Keynote: Evolution of Standardization in Laboratory Medicine
      Keynote: Evolution of Standardization in Laboratory Medicine
      There is agreement among clinicians that laboratory analyses are indispensable for correct diagnosis of disease, therapy, and patient monitoring. It is evident that laboratory data should be...
      Speaker: Linda Thienpont, PhD, PHARM, Clinical Chemist
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      4
      NOV 11, 2015 | 6:00 AM
      Clinical next-generation sequencing for precision cancer therapeutics
      Clinical next-generation sequencing for precision cancer therapeutics
      Next-generation sequencing has emerged as a valuable tool for generating patient-specific genetic information for clinical diagnostics and optimal selection of targeted therapies. The heterog...
      Speaker: Christina (Tina) Lockwood, PhD, DABCC, FACB
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      2
      NOV 11, 2015 | 6:00 AM
      A Comprehensive Toxicology Screening Solution Targeted to Individual Needs
      A Comprehensive Toxicology Screening Solution Targeted to Individual...
      Drugs of abuse toxicology testing by immunoassays is widely used clinically, but also known to be inadequate for many clinical indications. In this presentation, the speaker will outline diff...
      Speaker: Ping Wang, PhD, DABCC, FACB
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      3
      NOV 10, 2015 | 8:00 AM
      WEBINAR: Analysis of Chemical Contaminants in Drinking Water: Two techniques to analyze pesticides and disinfection products in drinking water in less time
      WEBINAR: Analysis of Chemical Contaminants in Drinking Water:...
      Date: Nov 10, 2015 8AM Pacific, 11AM Eastern, 4PM GMT Transporting smaller volumes of a sample, injecting less matrix, examining lower limits of detection and staying ahead of government r...
      Speaker: Jonathan Beck, PhD
      Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
      6 1
      OCT 22, 2015 | 8:00 AM
      WEBINAR: Exploring the Stoichiometry of Macromolecular Complexes Using Multi-Signal Sedimentation Velocity Analytical Ultracentrifugation
      WEBINAR: Exploring the Stoichiometry of Macromolecular Complexes...
      A fundamental aspect of the thermodynamic characterization of macromolecular complexes is the determination of the component stoichiometry. There are a number of approaches that may be employ...
      Speaker: Chad Brautigam, PhD
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      24
      OCT 01, 2015 | 12:00 PM
      Can the World's First Animal Study Software to Emulate Clinical Trial Approaches Improve Clinical Outcome Predictability?
      Can the World's First Animal Study Software to Emulate Clinical...
      The 95% failure rate of anticancer therapeutic being approved after positive Phase III trials suggests that existing pre-clinical approaches and tools are inadequate predictors of clinical ou...
      Speaker: Eric Ibsen
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
      3
      OCT 01, 2015 | 12:00 PM
      Detection and sequencing of rare mutations using mutation enrichment via COLD-PCR
      Detection and sequencing of rare mutations using mutation enrichment...
      Detection of rare mutations is required for reliable identification of mutations in liquid biopsies and heterogeneous tumors. Yet low-level mutation detection remains a technically challengin...
      Speaker: Mike Makrigiorgos, PhD, DABMP
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
      2
      OCT 01, 2015 | 9:30 AM
      WEBINAR: Extracellular Vesicle Isolation by Flow Cytometric Sorting and Characterization by Analytical Ultra-Centrifugation and Dynamic Light Scatter
      WEBINAR: Extracellular Vesicle Isolation by Flow Cytometric Sorting...
      Background:  The extracellular vesicle (EV) research field has dramatically increased in the last five years.  Using a high-speed flow cytometric sorter, EVs may be isolated at high...
      Speaker: Carley Ross, PhD
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      8
      OCT 01, 2015 | 8:00 AM
      WEBINAR: Close your uncertainty gap with High Resolution Mass Spectrometry
      WEBINAR: Close your uncertainty gap with High Resolution Mass...
      Is your lab constantly challenged by case back log while trying to validate new methods to identify designer drugs? Your challenge is common to many forensic toxicology labs around the world....
      Speaker: Maciej Bromirski, MSc , Kristine Van Natta
      Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
      2 1
      OCT 01, 2015 | 7:30 AM
      Latest clinical results in oncology drug management
      Latest clinical results in oncology drug management
      While TDM is routinely used in a number of medical fields, it has not gained wide utilization in oncology. There is a growing body of evidence demonstrating that current dosing methods based...
      Speaker: Salvatore Salamone, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
      2
      SEP 30, 2015 | 10:30 AM
      High efficiency SPRI (Solid Phase Reverse Immobilization Paramagnetic Bead) technology for microRNA applications
      High efficiency SPRI (Solid Phase Reverse Immobilization Paramagnetic...
      Micro RNAs (miRNAs) are small, naturally-occurring non-coding ribonucleic acids with sizes between 18 and 40 nucleotides (nts) that have been demonstrated to play a significant role in the re...
      Speaker: Bee Na Lee, PhD
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences
      2
      SEP 30, 2015 | 7:30 AM
      Ligand-Targeted Therapies for Cancer and Autoimmune Diseases
      Ligand-Targeted Therapies for Cancer and Autoimmune Diseases
      We have been developing methods to target drugs specifically to pathologic cells, thereby avoiding collateral toxicity to healthy cells. In the case of cancer, we have exploited up-regulation...
      Speaker: Philip Low, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
      2
      SEP 16, 2015 | 10:00 AM
      Catalyzing Translational Innovation
      Catalyzing Translational Innovation
      The process by which observations in the laboratory or the clinic are transformed into demonstrably useful interventions that tangibly improve human health is frequently termed "translation."...
      Speaker: Christopher Austin
      Presented at: C&EN's Virtual Symposium: Advances in Drug Discovery and Development
      2
      SEP 15, 2015 | 10:00 AM
      A new integrated platform for high quality and low cost gene expression analysis
      A new integrated platform for high quality and low cost gene expression...
      DATE: September 15, 2015TIME: 10:00AM Pacific time, 1:00PM Eastern timeThe breadth of instruments available for quantitative PCR (qPCR) has continued to grow in the past 5-10 years. With ...
      Speaker: Mikael Kubista , Ross Higgins
      Sponsored By: Douglas Scientific, Douglas Scientific
      4
    NOV 24, 2015 | 6:00 AM
    Say goodbye to Gels - Real world use of LabChip micro-fluidic technology for nucleic acid and protein analysis
    Say goodbye to Gels - Real world use of LabChip micro-fluidic...
    DATE: November 24, 2015TIME: 9am EST, 2pm GMTFor many years now, gels have been a fundamental research tool in academic research for the separation and analysis of nucleic acids and pro...
    Speaker: Julius Kostan, PhD , Sandra Doran , Veronika Delcheva
    Sponsored By: Revvity
    29
    NOV 19, 2015 | 7:00 AM
    WEBINAR: Glycated Hemoglobin (HbA1c) for Diagnosis and Monitoring of Diabetes: Current Practice
    WEBINAR: Glycated Hemoglobin (HbA1c) for Diagnosis and Monitoring...
    DATE: November 19, 2015 TIME: 7:00AM Pacific, 9:00AM Central, 10:00AM Eastern, 3:00PM GMT Diabetes represents a serious global epidemic.  Diabetes causes its own direct adverse ...
    Speaker: Cas Weykamp, PhD , Garry John, PhD
    Sponsored By: Abbott Molecular Diagnostic, Abbott Molecular Diagnostic
    10 10
    NOV 17, 2015 | 8:00 AM
    Unleashing the power of biobanks to drive translational science forward
    Unleashing the power of biobanks to drive translational science...
    DATE: November 17th, 2015TIME: 8am PDT, 11am EDT, 4pm GMTBiobanks provide important capacity to collect large genomic data sets intended to enable the study of genetic signatures of an...
    Speaker: Conny Mathay , Andrew Brooks, Ph.D. , Jens Hoefkens, PhD
    Sponsored By: Revvity
    22
    NOV 12, 2015 | 10:30 AM
    Pharmacogenetics in the Clinical Laboratory: Opportunities and Challenges
    Pharmacogenetics in the Clinical Laboratory: Opportunities and...
    Appropriate dosing of pharmaceuticals is critical to prevent sub-therapeutic efficacy or the occurrence of adverse events. However, genetic variability may significantly influence an individu...
    Speaker: Allison Chambliss, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    4
    NOV 12, 2015 | 9:00 AM
    Introducing an ancestrally diverse whole genome data set for select cohort and control research applications
    Introducing an ancestrally diverse whole genome data set for select...
    Introduction and adoption of whole-genome sequencing has enabled new methods of investigation in the quest for answers in diagnostic odyssey cases as well as in the broader study of genetic d...
    Speaker: Benjamin Solomon, MD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    3
     
     
  • NOV 12, 2015 | 7:30 AM
    Keynote: Reconceptualizing Genital Herpes Diagnosis and Management
    Keynote: Reconceptualizing Genital Herpes Diagnosis and Management
    As one of the nation's most prevalent sexually transmitted infections, genital herpes represents a diagnostic and therapeutic challenge to clinicians, laboratorians and the patients they...
    Speaker: Edward Hook, III, MD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    2 1
    NOV 11, 2015 | 1:30 PM
    Why to validate diagnostic tests?
    Why to validate diagnostic tests?
    This lecture will provide attendees with the knowledge and skills to bring on new clinical laboratory tests in clinical chemistry and molecular diagnostics. The lecture will provide a general...
    Speaker: Wieslaw Furmaga, MD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    4
    NOV 11, 2015 | 10:30 AM
    Reliability of LDL cholesterol: How low can we go and when are we fooling ourselves?
    Reliability of LDL cholesterol: How low can we go and when are...
    Aggressive low-density lipoprotein cholesterol (LDL-C) lowering strategies are recommended for prevention of cardiovascular events in high-risk populations. Guidelines recommend a 30-50% redu...
    Speaker: Jeffrey Meeusen, Ph.D., DABCC
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    2
    NOV 11, 2015 | 10:30 AM
    Role of LC/MS/MS spectrometry in diagnosis of various endocrine disorders
    Role of LC/MS/MS spectrometry in diagnosis of various endocrine...
        Until recently most of the phenotypic information on congenital endocrine disorders have relied on biochemical testing of steroids, biogenic amines and peptides but is now...
    Speaker: Ravinder Singh, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    0
    NOV 11, 2015 | 10:30 AM
    Agilent StreamSelect LC/MS System: Future solutions for the high throughput lab
    Agilent StreamSelect LC/MS System: Future solutions for the high...
    Liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) is ideally suited for the direct and rapid analysis of prepared biological samples. While analysis times can be shortened...
    Speaker: Kevin McCann, BS
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    5
     
     
  • NOV 11, 2015 | 7:30 AM
    Keynote: Evolution of Standardization in Laboratory Medicine
    Keynote: Evolution of Standardization in Laboratory Medicine
    There is agreement among clinicians that laboratory analyses are indispensable for correct diagnosis of disease, therapy, and patient monitoring. It is evident that laboratory data should be...
    Speaker: Linda Thienpont, PhD, PHARM, Clinical Chemist
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    4
    NOV 11, 2015 | 6:00 AM
    Clinical next-generation sequencing for precision cancer therapeutics
    Clinical next-generation sequencing for precision cancer therapeutics
    Next-generation sequencing has emerged as a valuable tool for generating patient-specific genetic information for clinical diagnostics and optimal selection of targeted therapies. The heterog...
    Speaker: Christina (Tina) Lockwood, PhD, DABCC, FACB
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    2
    NOV 11, 2015 | 6:00 AM
    A Comprehensive Toxicology Screening Solution Targeted to Individual Needs
    A Comprehensive Toxicology Screening Solution Targeted to Individual...
    Drugs of abuse toxicology testing by immunoassays is widely used clinically, but also known to be inadequate for many clinical indications. In this presentation, the speaker will outline diff...
    Speaker: Ping Wang, PhD, DABCC, FACB
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    3
    NOV 10, 2015 | 8:00 AM
    WEBINAR: Analysis of Chemical Contaminants in Drinking Water: Two techniques to analyze pesticides and disinfection products in drinking water in less time
    WEBINAR: Analysis of Chemical Contaminants in Drinking Water:...
    Date: Nov 10, 2015 8AM Pacific, 11AM Eastern, 4PM GMT Transporting smaller volumes of a sample, injecting less matrix, examining lower limits of detection and staying ahead of government r...
    Speaker: Jonathan Beck, PhD
    Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
    6 1
    OCT 22, 2015 | 8:00 AM
    WEBINAR: Exploring the Stoichiometry of Macromolecular Complexes Using Multi-Signal Sedimentation Velocity Analytical Ultracentrifugation
    WEBINAR: Exploring the Stoichiometry of Macromolecular Complexes...
    A fundamental aspect of the thermodynamic characterization of macromolecular complexes is the determination of the component stoichiometry. There are a number of approaches that may be employ...
    Speaker: Chad Brautigam, PhD
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    24
     
     
  • OCT 01, 2015 | 12:00 PM
    Can the World's First Animal Study Software to Emulate Clinical Trial Approaches Improve Clinical Outcome Predictability?
    Can the World's First Animal Study Software to Emulate Clinical...
    The 95% failure rate of anticancer therapeutic being approved after positive Phase III trials suggests that existing pre-clinical approaches and tools are inadequate predictors of clinical ou...
    Speaker: Eric Ibsen
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
    3
    OCT 01, 2015 | 12:00 PM
    Detection and sequencing of rare mutations using mutation enrichment via COLD-PCR
    Detection and sequencing of rare mutations using mutation enrichment...
    Detection of rare mutations is required for reliable identification of mutations in liquid biopsies and heterogeneous tumors. Yet low-level mutation detection remains a technically challengin...
    Speaker: Mike Makrigiorgos, PhD, DABMP
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
    2
    OCT 01, 2015 | 9:30 AM
    WEBINAR: Extracellular Vesicle Isolation by Flow Cytometric Sorting and Characterization by Analytical Ultra-Centrifugation and Dynamic Light Scatter
    WEBINAR: Extracellular Vesicle Isolation by Flow Cytometric Sorting...
    Background:  The extracellular vesicle (EV) research field has dramatically increased in the last five years.  Using a high-speed flow cytometric sorter, EVs may be isolated at high...
    Speaker: Carley Ross, PhD
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    8
    OCT 01, 2015 | 8:00 AM
    WEBINAR: Close your uncertainty gap with High Resolution Mass Spectrometry
    WEBINAR: Close your uncertainty gap with High Resolution Mass...
    Is your lab constantly challenged by case back log while trying to validate new methods to identify designer drugs? Your challenge is common to many forensic toxicology labs around the world....
    Speaker: Maciej Bromirski, MSc , Kristine Van Natta
    Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
    2 1
    OCT 01, 2015 | 7:30 AM
    Latest clinical results in oncology drug management
    Latest clinical results in oncology drug management
    While TDM is routinely used in a number of medical fields, it has not gained wide utilization in oncology. There is a growing body of evidence demonstrating that current dosing methods based...
    Speaker: Salvatore Salamone, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
    2
     
     
  • SEP 30, 2015 | 10:30 AM
    High efficiency SPRI (Solid Phase Reverse Immobilization Paramagnetic Bead) technology for microRNA applications
    High efficiency SPRI (Solid Phase Reverse Immobilization Paramagnetic...
    Micro RNAs (miRNAs) are small, naturally-occurring non-coding ribonucleic acids with sizes between 18 and 40 nucleotides (nts) that have been demonstrated to play a significant role in the re...
    Speaker: Bee Na Lee, PhD
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences
    2
    SEP 30, 2015 | 7:30 AM
    Ligand-Targeted Therapies for Cancer and Autoimmune Diseases
    Ligand-Targeted Therapies for Cancer and Autoimmune Diseases
    We have been developing methods to target drugs specifically to pathologic cells, thereby avoiding collateral toxicity to healthy cells. In the case of cancer, we have exploited up-regulation...
    Speaker: Philip Low, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
    2
    SEP 16, 2015 | 10:00 AM
    Catalyzing Translational Innovation
    Catalyzing Translational Innovation
    The process by which observations in the laboratory or the clinic are transformed into demonstrably useful interventions that tangibly improve human health is frequently termed "translation."...
    Speaker: Christopher Austin
    Presented at: C&EN's Virtual Symposium: Advances in Drug Discovery and Development
    2
    SEP 15, 2015 | 10:00 AM
    A new integrated platform for high quality and low cost gene expression analysis
    A new integrated platform for high quality and low cost gene expression...
    DATE: September 15, 2015TIME: 10:00AM Pacific time, 1:00PM Eastern timeThe breadth of instruments available for quantitative PCR (qPCR) has continued to grow in the past 5-10 years. With ...
    Speaker: Mikael Kubista , Ross Higgins
    Sponsored By: Douglas Scientific, Douglas Scientific
    4
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