CRISPR is an abbreviation of Clustered Regularly Interspaced Short Palindromic Repeats. The name was minted at a time when the origin and use of the interspacing subsequences were not known. At that time the CRISPRs were described as segments of prokaryotic DNA containing short, repetitive base sequences. In a palindromic repeat, the sequence of nucleotides is the same in both directions. Each repetition is followed by short segments of spacer DNA from previous exposures to foreign DNA (e.g., a virus or plasmid). Small clusters of cas (CRISPR-associated) genes are located next to CRISPR sequences.
This webinar explains how CAR-T cells transfected using Solupore® technology from Avectas and sorted using the CGX10 Cell Isolation System from Sony Biotechnology outperform unsorted cel...
From Discovery to Clinic: Comprehensive CRISPR Solutions for Therapeutic Development CRISPR gene editing has revolutionized the field of molecular biology, offering precise, efficient, and v...
CRISPR-Cas9 has been widely adapted for use in transcriptional modulation and epigenetic engineering with deactivated Cas9 (dCas9) systems to enable CRISPR interference (CRISPRi) and CRISPR...
The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated proteins (Cas) system is a bacterial defense mechanism that uses RNA-guided enzymes to cut specific s...
Advanced Gene Therapy Development Solutions: A Comprehensive Platform for Next-Generation Therapeutic Innovation: The landscape of gene therapy has evolved dramatically over the past decade,...
In recent years, non-viral methods for cell engineering have emerged as promising alternatives to viral transduction. Amongst the virus-free technologies, electroporation is considered the g...
Ebola virus executes its replication cycle through a tightly orchestrated sequence of events, but the host factors that govern the timing of these steps have remained poorly defined. In this...
The adult mammalian heart bears limited regenerative capacity, resulting in the irreversible loss of cardiomyocytes post-cardiac injury, and often culminating in end-stage heart failure. Car...
In this webinar, we expand on how the innovations of NovaSeq X are making multiomics a reality. We will highlight data from multi-project runs, massive single cell and CRISPR applications, a...
While hundreds of different lipids have been identified in human blood, many of them remain functionally uncharacterized, including a class of lipids called long-chain acylcarnitines (LCACs)...
BrainXell offers the most comprehensive portfolio of high-purity iPSC-derived human neurons and glia, sourced from control, edited and disease lines, to advance translational research and dr...
Single cell isolation is a necessary step for a variety of applications from developing productive, stable cell lines for biologics manufacturing to developing CRISPR edited iPSCs for diseas...
The SH800 Cell Sorter by Sony is a versatile solution designed to deliver exceptional sorting performance across a wide array of applications. Tailored to meet diverse user needs, it incorpo...
Are low gene editing efficiency or gene expression levels preventing your research from advancing? Is your transfection method killing your cells? Are you struggling to reproduce your initia...
Identifying and verifying low frequencies of genomic alterations resulting from off-target editing, gRNA synthesis errors, cross-contamination, or other unintended gRNA activity is critical...
CRISPR has emerged as a promising alternative to viral-based gene editing by allowing scientists to modify genes with unprecedented ease and flexibility. The discovery and development of a d...
This talk will provide an overview of our work in the design of self-assembled, functionalised peptide and protein nanoparticles, hydrogels and bio-interfaces for applications in healthcare....
The Myhrvold lab develops CRISPR-based technologies for studying RNA. Our primary interest is RNAs involved in host-pathogen interactions. We use a variety of RNA viruses and other pathogens...
Originally developed for gene editing, CRISPR-Cas systems have now become powerful molecular diagnostic tools. However, their widespread adoption in diagnostics has relied heavily on traditi...
Gene therapy involves introducing a functional gene into the patient's cells to replace a missing or defective one. It has revolutionized the field of medicine and has the potential to c...
Pancreatic ductal adenocarcinoma (PDAC) is characterized clinically by poor survival and mechanistically by KRAS- and autophagy-dependent growth. We and others previously demonstrated that i...
Abstract: Beginning with a brief historical overview of CRISPR, we will trace its evolution to the current state of gene engineering, highlighting its pivotal role in reshaping the landscape...
Gene therapy has revolutionized the field of medicine, and it has the potential to cure a wide variety of genetic disorders. Gene therapy involves the introduction of a functional gene into...
Learning Objevtives: 1. Demonstrate Knowledge with gene editing, microfluidics and non-viral delivery methods. 2. Review the uses of a low-volume high-throughput transfection platform for ge...
This webinar explains how CAR-T cells transfected using Solupore® technology from Avectas and sorted using the CGX10 Cell Isolation System from Sony Biotechnology outperform unsorted cel...
From Discovery to Clinic: Comprehensive CRISPR Solutions for Therapeutic Development CRISPR gene editing has revolutionized the field of molecular biology, offering precise, efficient, and v...
CRISPR-Cas9 has been widely adapted for use in transcriptional modulation and epigenetic engineering with deactivated Cas9 (dCas9) systems to enable CRISPR interference (CRISPRi) and CRISPR...
The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated proteins (Cas) system is a bacterial defense mechanism that uses RNA-guided enzymes to cut specific s...
Advanced Gene Therapy Development Solutions: A Comprehensive Platform for Next-Generation Therapeutic Innovation: The landscape of gene therapy has evolved dramatically over the past decade,...
In recent years, non-viral methods for cell engineering have emerged as promising alternatives to viral transduction. Amongst the virus-free technologies, electroporation is considered the g...
Ebola virus executes its replication cycle through a tightly orchestrated sequence of events, but the host factors that govern the timing of these steps have remained poorly defined. In this...
The adult mammalian heart bears limited regenerative capacity, resulting in the irreversible loss of cardiomyocytes post-cardiac injury, and often culminating in end-stage heart failure. Car...
In this webinar, we expand on how the innovations of NovaSeq X are making multiomics a reality. We will highlight data from multi-project runs, massive single cell and CRISPR applications, a...
While hundreds of different lipids have been identified in human blood, many of them remain functionally uncharacterized, including a class of lipids called long-chain acylcarnitines (LCACs)...
BrainXell offers the most comprehensive portfolio of high-purity iPSC-derived human neurons and glia, sourced from control, edited and disease lines, to advance translational research and dr...
Single cell isolation is a necessary step for a variety of applications from developing productive, stable cell lines for biologics manufacturing to developing CRISPR edited iPSCs for diseas...
The SH800 Cell Sorter by Sony is a versatile solution designed to deliver exceptional sorting performance across a wide array of applications. Tailored to meet diverse user needs, it incorpo...
Are low gene editing efficiency or gene expression levels preventing your research from advancing? Is your transfection method killing your cells? Are you struggling to reproduce your initia...
Identifying and verifying low frequencies of genomic alterations resulting from off-target editing, gRNA synthesis errors, cross-contamination, or other unintended gRNA activity is critical...
CRISPR has emerged as a promising alternative to viral-based gene editing by allowing scientists to modify genes with unprecedented ease and flexibility. The discovery and development of a d...
This talk will provide an overview of our work in the design of self-assembled, functionalised peptide and protein nanoparticles, hydrogels and bio-interfaces for applications in healthcare....
The Myhrvold lab develops CRISPR-based technologies for studying RNA. Our primary interest is RNAs involved in host-pathogen interactions. We use a variety of RNA viruses and other pathogens...
Originally developed for gene editing, CRISPR-Cas systems have now become powerful molecular diagnostic tools. However, their widespread adoption in diagnostics has relied heavily on traditi...
Gene therapy involves introducing a functional gene into the patient's cells to replace a missing or defective one. It has revolutionized the field of medicine and has the potential to c...
Pancreatic ductal adenocarcinoma (PDAC) is characterized clinically by poor survival and mechanistically by KRAS- and autophagy-dependent growth. We and others previously demonstrated that i...
Abstract: Beginning with a brief historical overview of CRISPR, we will trace its evolution to the current state of gene engineering, highlighting its pivotal role in reshaping the landscape...
Gene therapy has revolutionized the field of medicine, and it has the potential to cure a wide variety of genetic disorders. Gene therapy involves the introduction of a functional gene into...
Learning Objevtives: 1. Demonstrate Knowledge with gene editing, microfluidics and non-viral delivery methods. 2. Review the uses of a low-volume high-throughput transfection platform for ge...
Follow
